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Research Article

volume 43 issue 6 (december 2020) : 788-793,   Doi: 10.18805/LR-536

Influence of Boron Priming on the Antioxidant Ability of Alfalfa Seeds

F
F.S. Xia
F
F. Wang
Y
Y.C. Wang
C
C.C. Wang
R
R. Tian
J
J.Y. Ma
H
H.S. Zhu
K
K.H. Dong
1College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China.

  • Submitted22-11-2019|

  • Accepted15-03-2020|

  • First Online 15-07-2020|

  • doi 10.18805/LR-536

Cite article:- Xia F.S., Wang F., Wang Y.C., Wang C.C., Tian R., Ma J.Y., Zhu H.S., Dong K.H. (2020). Influence of Boron Priming on the Antioxidant Ability of Alfalfa Seeds. Legume Research. 43(6): 788-793. doi: 10.18805/LR-536.

ABSTRACT

This experiment was designed to determine the relationship between the antioxidant capacity and the vigour of alfalfa seeds during boron priming. Alfalfa seeds were primed with 1.8 % (W/V) concentration of borax solution for 0, 3, 6, 12 and 24 h at 20°C. The results showed that the vigour of alfalfa seeds declined with the extension of priming time, which was closely related to the accumulation of hydrogen peroxide and malonaldehyde contents and the decrease of superoxide dismutase, catalase and peroxidase. The radicle might be the most sensitive site to boron priming in alfalfa seeds, but the boron-induced damage might be alleviated during alfalfa seeds’ germination.

REFERENCES

  1. Abdul-Baki, A.A. and Anderson, J.D. (1973). Vigour determination in soybean seed multiple criteria. Crop Science. 13: 630-633.
  2. Aebi, H.E. (1986). Catalase. In methods of enzymatic analysis. Verlag Chemie, Weinheim. pp. 273-286.
  3. Al-Kahtani, S.N., Taha, A.E., Al-Abdulsalam, M. (2017). Alfalfa (Medicago sativa L.) seed yield in relation to phosphorus fertilization and honeybee pollination. Saudi Journal of Biological Sciences. 24: 1051-1055.
  4. Anon (2017) International Rules for Seed Testing: The germination test. International Seed Testing Association, Zurich. pp. 25.
  5. Atique-ur-Rehman, Farooq, M., Nawaz, A., Ahmad, R. (2014). Influence of boron nutrition on the rice productivity, kernel quality and biofortification in different production systems. Field Crops Research. 169: 123-131.
  6. Bailly, C., Benamar, A., Corbineau, F., Côme, D. (1996). Changes in malondialdehyde content and superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiologia Plantarum. 97: 104-110.
  7. Basra, S.M., Farooq, M., Tabassam, R., Ahmad, N. (2005). Physiological and biochemical aspects of pre-sowing seed treatments in fine rice (Oryza sativa L.). Seed Science and Technology. 33: 623-628.
  8. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytic Biochemistry. 72: 248-254.
  9. Chen, K. and Arora, R. (2013). Priming memory invokes seed stress-tolerance. Environment and Experimental Botany. 94: 33-45.
  10. Du, W.H., Tian, X.H., Cao, Z.Z., Humphries, A. (2009). Effects of micronutrients on seed yield and yield components of alfalfa. Journal of Plant Nutrition. 32: 809-820.
  11. Eggert, K. and von Wirén, N. (2016). The role of boron nutrition in seed vigour of oil seed rape (Brassica napus L.). Plant Soil. 402: 63-76.
  12. Ellis, R.H., Osei-Bonsu, K., Roberts, E.H. (1982). The influence of genotype, temperature and moisture on seed longevity in chickpea, cowpea and soyabean. Annals of Botany. 50: 69-82. 
  13. Farooq, M., Rehman, A., Aziz, T., Habib, M. (2011). Boron nutripriming improves the germination and early seedling growth of rice (Oryza sativa L.). Journal of Plant Nutrition. 34: 1507-1515.
  14. Farooq, M., Wahid, A., Siddiqu, K.H.M. (2012). Micronutrient application through seed treatments-a review. Journal of Soil Science and Plant Nutrition. 12: 125-142.
  15. Herrera-Rodriguez, M.B., Gonzalez-Fontes, A., Rexach, J., Camacho -Cristobal, J.J., Maldonado, J.M., Navarro-Gochicao, M.T. (2010). Role of boron in vascular plants and response mechanism to boron stresses. Plant Stress. 4: 115-122.
  16. Huang, Z., Liu, Y., Cui, Z., Fang, Y., He, H., Liu, B., Wu, G. (2018). Soil water storage deficit of alfalfa (Medicago sativa) grasslands along ages in arid area (China). Field Crops Research. 221: 1-6.
  17. Iqbal, S., Farooq, M., Cheema, S.A., Afzal, I. (2017). Boron seed priming improves the seedling emergence, growth, grain yield and grain biofortification of bread wheat. International Journal of Agriculture and Biology. 19: 177-182.
  18. Jisha, K.C. and Puthur, J.T. (2016). Seed priming with beta-amino butyric acid improves abiotic stress tolerance in rice seedlings. Rice Science. 23: 242-254.
  19. Jr Mattos, D., Hippler, F.W.R., Boaretto, R.M., Stuchi, E.S., Quaggio, J.A. (2017). Soil boron fertilization: The role of nutrient sources and rootstocks in citrus production. Journal of Integrative Agriculture. 16: 1609-1616.
  20. Kalpana, R. and Rao, K.V. (1995). On the aging mechanism in pigeonpea (Cajanus cajan L.) seed. Seed Science and Technology. 23: 1-9.
  21. Karabal, E., Yücel, M., Öktem, H.A. (2003). Antioxidant responses of tolerant and sensitive barley cultivars to boron toxixity. Plant Science. 164: 925-933.
  22. Kaya, C. and Ashraf, M. (2015). Exogenous application of nitric oxide promotes growth and oxidative defense system in highly boron stressed tomato plants bearing fruit. Scientia Horticulturae. 185: 43-47.
  23. Kibinza, S., Vinel, D., Côme, D., Bailly, C., Corbineau, F. (2006). Sunflower seed deterioration as related to moisture         content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum. 128: 496-506.
  24. Molassiotis, A., Sotiropoulos, T., Tanou, G., Diamantidis, G., Therios, I. (2006). Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM 9 (Malus domestica Borkh). Environmental and Experimental Botany. 56: 54-62.
  25. Moulick, D., Santra, S.C., Ghosh, D. (2018). Seed priming with Se mitigates As-induced phytotoxicity in rice seedlings by enhancing essential micronutrient uptake and translocation and reducing as translocation. Environmental Science and         Pollution Research. 25: 26978-26991.
  26. Nadeem, F., Farooq, M., Nawaz, A., Ahmad, R. (2019). Boron improves productivity and profitability of bread wheat under zero and plough tillage on alkaline calcareous soil.Field Crops Research. 239: 1-9.
  27. Panse, V.G. and Sukhatme, P.V. (1967). Statistical Methods for Agricultural Workers, 2nd Edition, ICAR publication, New Delhi. pp. 152-157. 
  28. Parry, F.A., Chattoo, M.A., Ganie, S.A., Razvi, S.M. (2016). Economics of seed production in garden pea (Pisum sativum L.) as influenced by different levels of sulphur and boron. Legume Research. 39: 802-805.
  29. Patterson, B.D., Macrae, E.A., Ferguson, I.B. (1984). Estimation of hydrogen peroxide in plants extracts using titanium (IV). Analytical Biochemistry. 139: 487-492.
  30. Paul, S. and Roychoudhury. A. (2017). Seed priming with spermine and spermidine regulates the expression of diverse groups of abiotic stress-responsive genes during salinity stress in the seedlings of indica rice varieties. Plant Gene. 11: 124-132.
  31. Rao, K.V.M. and Sresty, T.V.S. (2000). Antioxidant parameters in the seedlings of pigeon pea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Science. 157: 113-128.
  32. Xia, F.S., Chen, L.L., Ya, H.F., Sun, Y., Li, M.L., Mao, P.S. (2016). Antioxidant and ultrastructural responses to priming with PEG in aged, ultra-dry oat seed. Seed Science and Technology. 44: 1-13.
  33. Xia, F.S., Wang, M.Y., Chen, L.L., Cheng, H., Sun, Y., Li, M.L., Dong. K.H., Zhao, X., Mao, P.S. (2017). Responses of mitochondrial ultrastructure and physiological variations to PEG-priming on ultra-dried oat (Avena sativa L.) seeds after ageing. Seed Science and Technology. 45: 622-637.
  34. Xia, F.S., Wang, Y.C., Zhu, H.S., Ma, J.Y., Yang, Y.Y., Tian, R., Dong, K.H. (2019). Influence of priming with exogenous boron on the seed vigour of alfalfa (Medicago sativa L.). Legume Research. 42: 795-799.
  35. Zhang, W., Liang, L., Zhang, X., Chen, J., Wang, H., Mao, P.  (2019). Influence of alfalfa seed belts on yield component and seed yield in mainland China - A review. Legume Research. 42: 723-728.
  36. Zhang, W., Xia, F., Li, Y., Wang, M., Mao, P. (2016). Influence of year and row spacing on yield component and seed yield in alfalfa (Medicago sativa L.). Legume Research. 292: 1-6. 
  37. Zhao, Y., Hu, M., Gao, Z., Chen, X., Huang, D. (2018). Biological mechanisms of a novel hydro-electro hybrid priming         recovers potential vigor of onion seeds. Environmental and Experimental Botany. 150: 260-271.
  38. Zhu, H. and Bañuelos, G. (2016). Influence of salinity and boron on germination, seedling growth andtransplanting mortality of guayule: A combined growth chamber andgreenhouse study. Industrial Crops and Products. 92: 236-243.
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    Research Article

    volume 43 issue 6 (december 2020) : 788-793,   Doi: 10.18805/LR-536

    Influence of Boron Priming on the Antioxidant Ability of Alfalfa Seeds

    F
    F.S. Xia
    F
    F. Wang
    Y
    Y.C. Wang
    C
    C.C. Wang
    R
    R. Tian
    J
    J.Y. Ma
    H
    H.S. Zhu
    K
    K.H. Dong
    1College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China.

    • Submitted22-11-2019|

    • Accepted15-03-2020|

    • First Online 15-07-2020|

    • doi 10.18805/LR-536

    Cite article:- Xia F.S., Wang F., Wang Y.C., Wang C.C., Tian R., Ma J.Y., Zhu H.S., Dong K.H. (2020). Influence of Boron Priming on the Antioxidant Ability of Alfalfa Seeds. Legume Research. 43(6): 788-793. doi: 10.18805/LR-536.

    ABSTRACT

    This experiment was designed to determine the relationship between the antioxidant capacity and the vigour of alfalfa seeds during boron priming. Alfalfa seeds were primed with 1.8 % (W/V) concentration of borax solution for 0, 3, 6, 12 and 24 h at 20°C. The results showed that the vigour of alfalfa seeds declined with the extension of priming time, which was closely related to the accumulation of hydrogen peroxide and malonaldehyde contents and the decrease of superoxide dismutase, catalase and peroxidase. The radicle might be the most sensitive site to boron priming in alfalfa seeds, but the boron-induced damage might be alleviated during alfalfa seeds’ germination.

    REFERENCES

    1. Abdul-Baki, A.A. and Anderson, J.D. (1973). Vigour determination in soybean seed multiple criteria. Crop Science. 13: 630-633.
    2. Aebi, H.E. (1986). Catalase. In methods of enzymatic analysis. Verlag Chemie, Weinheim. pp. 273-286.
    3. Al-Kahtani, S.N., Taha, A.E., Al-Abdulsalam, M. (2017). Alfalfa (Medicago sativa L.) seed yield in relation to phosphorus fertilization and honeybee pollination. Saudi Journal of Biological Sciences. 24: 1051-1055.
    4. Anon (2017) International Rules for Seed Testing: The germination test. International Seed Testing Association, Zurich. pp. 25.
    5. Atique-ur-Rehman, Farooq, M., Nawaz, A., Ahmad, R. (2014). Influence of boron nutrition on the rice productivity, kernel quality and biofortification in different production systems. Field Crops Research. 169: 123-131.
    6. Bailly, C., Benamar, A., Corbineau, F., Côme, D. (1996). Changes in malondialdehyde content and superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiologia Plantarum. 97: 104-110.
    7. Basra, S.M., Farooq, M., Tabassam, R., Ahmad, N. (2005). Physiological and biochemical aspects of pre-sowing seed treatments in fine rice (Oryza sativa L.). Seed Science and Technology. 33: 623-628.
    8. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytic Biochemistry. 72: 248-254.
    9. Chen, K. and Arora, R. (2013). Priming memory invokes seed stress-tolerance. Environment and Experimental Botany. 94: 33-45.
    10. Du, W.H., Tian, X.H., Cao, Z.Z., Humphries, A. (2009). Effects of micronutrients on seed yield and yield components of alfalfa. Journal of Plant Nutrition. 32: 809-820.
    11. Eggert, K. and von Wirén, N. (2016). The role of boron nutrition in seed vigour of oil seed rape (Brassica napus L.). Plant Soil. 402: 63-76.
    12. Ellis, R.H., Osei-Bonsu, K., Roberts, E.H. (1982). The influence of genotype, temperature and moisture on seed longevity in chickpea, cowpea and soyabean. Annals of Botany. 50: 69-82. 
    13. Farooq, M., Rehman, A., Aziz, T., Habib, M. (2011). Boron nutripriming improves the germination and early seedling growth of rice (Oryza sativa L.). Journal of Plant Nutrition. 34: 1507-1515.
    14. Farooq, M., Wahid, A., Siddiqu, K.H.M. (2012). Micronutrient application through seed treatments-a review. Journal of Soil Science and Plant Nutrition. 12: 125-142.
    15. Herrera-Rodriguez, M.B., Gonzalez-Fontes, A., Rexach, J., Camacho -Cristobal, J.J., Maldonado, J.M., Navarro-Gochicao, M.T. (2010). Role of boron in vascular plants and response mechanism to boron stresses. Plant Stress. 4: 115-122.
    16. Huang, Z., Liu, Y., Cui, Z., Fang, Y., He, H., Liu, B., Wu, G. (2018). Soil water storage deficit of alfalfa (Medicago sativa) grasslands along ages in arid area (China). Field Crops Research. 221: 1-6.
    17. Iqbal, S., Farooq, M., Cheema, S.A., Afzal, I. (2017). Boron seed priming improves the seedling emergence, growth, grain yield and grain biofortification of bread wheat. International Journal of Agriculture and Biology. 19: 177-182.
    18. Jisha, K.C. and Puthur, J.T. (2016). Seed priming with beta-amino butyric acid improves abiotic stress tolerance in rice seedlings. Rice Science. 23: 242-254.
    19. Jr Mattos, D., Hippler, F.W.R., Boaretto, R.M., Stuchi, E.S., Quaggio, J.A. (2017). Soil boron fertilization: The role of nutrient sources and rootstocks in citrus production. Journal of Integrative Agriculture. 16: 1609-1616.
    20. Kalpana, R. and Rao, K.V. (1995). On the aging mechanism in pigeonpea (Cajanus cajan L.) seed. Seed Science and Technology. 23: 1-9.
    21. Karabal, E., Yücel, M., Öktem, H.A. (2003). Antioxidant responses of tolerant and sensitive barley cultivars to boron toxixity. Plant Science. 164: 925-933.
    22. Kaya, C. and Ashraf, M. (2015). Exogenous application of nitric oxide promotes growth and oxidative defense system in highly boron stressed tomato plants bearing fruit. Scientia Horticulturae. 185: 43-47.
    23. Kibinza, S., Vinel, D., Côme, D., Bailly, C., Corbineau, F. (2006). Sunflower seed deterioration as related to moisture         content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum. 128: 496-506.
    24. Molassiotis, A., Sotiropoulos, T., Tanou, G., Diamantidis, G., Therios, I. (2006). Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM 9 (Malus domestica Borkh). Environmental and Experimental Botany. 56: 54-62.
    25. Moulick, D., Santra, S.C., Ghosh, D. (2018). Seed priming with Se mitigates As-induced phytotoxicity in rice seedlings by enhancing essential micronutrient uptake and translocation and reducing as translocation. Environmental Science and         Pollution Research. 25: 26978-26991.
    26. Nadeem, F., Farooq, M., Nawaz, A., Ahmad, R. (2019). Boron improves productivity and profitability of bread wheat under zero and plough tillage on alkaline calcareous soil.Field Crops Research. 239: 1-9.
    27. Panse, V.G. and Sukhatme, P.V. (1967). Statistical Methods for Agricultural Workers, 2nd Edition, ICAR publication, New Delhi. pp. 152-157. 
    28. Parry, F.A., Chattoo, M.A., Ganie, S.A., Razvi, S.M. (2016). Economics of seed production in garden pea (Pisum sativum L.) as influenced by different levels of sulphur and boron. Legume Research. 39: 802-805.
    29. Patterson, B.D., Macrae, E.A., Ferguson, I.B. (1984). Estimation of hydrogen peroxide in plants extracts using titanium (IV). Analytical Biochemistry. 139: 487-492.
    30. Paul, S. and Roychoudhury. A. (2017). Seed priming with spermine and spermidine regulates the expression of diverse groups of abiotic stress-responsive genes during salinity stress in the seedlings of indica rice varieties. Plant Gene. 11: 124-132.
    31. Rao, K.V.M. and Sresty, T.V.S. (2000). Antioxidant parameters in the seedlings of pigeon pea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Science. 157: 113-128.
    32. Xia, F.S., Chen, L.L., Ya, H.F., Sun, Y., Li, M.L., Mao, P.S. (2016). Antioxidant and ultrastructural responses to priming with PEG in aged, ultra-dry oat seed. Seed Science and Technology. 44: 1-13.
    33. Xia, F.S., Wang, M.Y., Chen, L.L., Cheng, H., Sun, Y., Li, M.L., Dong. K.H., Zhao, X., Mao, P.S. (2017). Responses of mitochondrial ultrastructure and physiological variations to PEG-priming on ultra-dried oat (Avena sativa L.) seeds after ageing. Seed Science and Technology. 45: 622-637.
    34. Xia, F.S., Wang, Y.C., Zhu, H.S., Ma, J.Y., Yang, Y.Y., Tian, R., Dong, K.H. (2019). Influence of priming with exogenous boron on the seed vigour of alfalfa (Medicago sativa L.). Legume Research. 42: 795-799.
    35. Zhang, W., Liang, L., Zhang, X., Chen, J., Wang, H., Mao, P.  (2019). Influence of alfalfa seed belts on yield component and seed yield in mainland China - A review. Legume Research. 42: 723-728.
    36. Zhang, W., Xia, F., Li, Y., Wang, M., Mao, P. (2016). Influence of year and row spacing on yield component and seed yield in alfalfa (Medicago sativa L.). Legume Research. 292: 1-6. 
    37. Zhao, Y., Hu, M., Gao, Z., Chen, X., Huang, D. (2018). Biological mechanisms of a novel hydro-electro hybrid priming         recovers potential vigor of onion seeds. Environmental and Experimental Botany. 150: 260-271.
    38. Zhu, H. and Bañuelos, G. (2016). Influence of salinity and boron on germination, seedling growth andtransplanting mortality of guayule: A combined growth chamber andgreenhouse study. Industrial Crops and Products. 92: 236-243.
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