Legume Research

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Research Article

Legume Research, volume 45 issue 10 (october 2022) : 1209-1215

Cross-species Amplification and Molecular Diversity Analysis in Greengram [Vigna radiata (L.) Wilczek] and Ricebean [Vigna umbellata Thunb.]

D. Susmitha, P. Jayamani
1Department of Pulses, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.

  • Submitted06-09-2019|

  • Accepted29-09-2020|

  • First Online 16-12-2020|

  • doi 10.18805/LR-4230

Cite article:- Susmitha D., Jayamani P. (2022). Cross-species Amplification and Molecular Diversity Analysis in Greengram [Vigna radiata (L.) Wilczek] and Ricebean [Vigna umbellata Thunb.]. Legume Research. 45(10): 1209-1215. doi: 10.18805/LR-4230.

ABSTRACT

Background: Greengram is the fourth dominant pulse crop grown in India and is highly susceptible to bruchids and yellow mosaic virus. Ricebean belonging to the tertiary genepool of greengram shows resistance to this pest and disease. Ricebean being a minor pulse crop has limited SSR markers. Hence, the present study was conducted to test the cross-species amplification of SSR markers derived from related species which could be helpful in studying the molecular diversity available in each crop and for molecular confirmation of the inter- and intra-specific hybrid developed from the crosses.
Methods: Molecular diversity analysis was carried out using 30 genotypes belonging to two species of Vigna (V. radiata and V. umbellata) based on 20 SSR primers derived from adzukibean, commonbean and greengram. 
Result: Cross species amplification was observed for all the SSR primers pairs used. The number of alleles detected varied from one (VJ 31122A) to four (CEDAAG 001 and MB 91) with an average of 2.55 alleles per primer. Allele size varied from 100 to 292 bp. The PIC value ranged from zero to 0.611 with an average value of 0.377. Dendrograms constructed based on UPGMA and Neighbour-Joining tree method, grouped the genotypes into five clusters and five groups, respectively. The V. umbellata genotypes were grouped in separate cluster from the V. radiata genotypes in both the methods. The obtained DNA polymorphism at intra- and inter-specific level will facilitate the application of molecular breeding approaches for greengram improvement. 

REFERENCES

  1. Azevedo, A.L.S., Costa, P.P., Machado, J.C., Machado, M.A., Vander Pereira, A. and da Silva Lédo, F.J. (2012). Cross species amplification of Pennisetum glaucum microsatellite markers in Pennisetum purpureum and genetic diversity of napier grass accessions. Crop Science. 52(4): 1776-1785.
  2. Chen, H., Qiao, L., Wang, L., Wang, S., Blair, M.W. and Cheng, X. (2015). Assessment of genetic diversity and population structure of mung bean (Vigna radiata) germplasm using EST-based and genomic SSR markers. Gene. 566(2): 175-183. 
  3. Gwag, G, J., Chung, J.W., Chung, H.K., Lee, J.H., Ma, K.H., et al. (2006). Characterization of new microsatellite markers in mung bean,Vigna radiata (L.). Molecular Ecology Resources. 6(4): 1132-1134. 
  4. Gwag, J.G., Dixit, A., Park, Y.J., Ma, K.H., Kwon, S.J., Cho, G.T. and Lee, S.H. (2010). Assessment of genetic diversity and population structure in mungbean. Genes and Genomics. 32(4): 299-308. 
  5. Harouna, D.V., Venkataramana, P.B., Matemu, A.O. and Ndakidemi, P.A. (2019). Wild Vigna legumes: farmers’ perceptions, preferences and prospective uses for human exploitation. Agronomy. 9(6): 284.
  6. https://www.indiastat.com/table/agriculture-data/2/green-gram/19571/1229723/data. aspx.
  7. Jarne, P. and Lagoda, P.J. (1996). Microsatellites, from molecules to populations and back. Trends in Ecology and Evolution. 11(10): 424-429. 
  8. Jayamani, P. and Sathya, M. (2012). Genetic diversity as assessed by morphological and microsatellite markers in greengram (Vigna radiata L.). African Journal of Biotechnology. 11 (84): 15091-15097. 
  9. Jena, B., Panigrahi, K., Baisakh, B. and Das, T. (2015). Genetic Diversity as Assessed by Agronomical Traits and Molecular Markers in Local Land Races of Green gram (Vigna radiata L.) Under Rice Fallow Condition. Molecular Plant Breeding. 6(8): 1-7. 
  10. Kumar, S., Tan, S., Quah, S. and Yusoff, K. (2002). Isolation and characterization of seven tetranucleotide microsatellite loci in mungbean, Vigna radiata. Molecular Ecology Resources. 2(3): 293-295. 
  11. Li-Xia, W., Cheng, X.Z., Su-Hua, W., Chang-You, L. and Liang, H. (2009). Transferability of SSR markers from adzuki bean into mungbean. Acta Agronomica Sinica. 35(5): 816-820. 
  12. Mignouna, H., Ikea, J., Thottapilly, G. and Ng, N. (1998). Genetic diversity in cowpea as revealed by random amplified polymorphic DNA [RAPD-Vigna unguiculata (L.) Walp]. Journal of Genetics and Breeding (Italy). 52: 151-159. 
  13. Perrier, X. and Jaqeuemond-Collet, J.P. (2005). Darwin- 5.0 software; Dissimilarity analysis and representation for windows. France: Equipe Mathematique et Informatique. 
  14. Sathya, M. and Jayamani, P. (2013). Cross Species Amplification of Adzukibean Derived Microsatellite Loci and Diversity Analysis in Greengram and Related Vigna Species. Molecular Plant Breeding. 4(11): 89-95. 
  15. Shivakumar, M.S. and Ramesh, S. (2015). Transferability of Cross Legume Species/Genera SSR Markers to Dolichos Bean (Lablab purpureus L. Sweet) var. Lignosus. Ecialise S. 49(2): 263-265.
  16. Shrivastava, D., Verma, P. and Bhatia, S. (2014). Expanding the repertoire of microsatellite markers for polymorphism studies in Indian accessions of mung bean [Vigna radiata (L.) Wilczek]. Molecular biology reports. 41(9): 5669-5680. 
  17. Singh, I., Sandhu, J.S., Gupta, S.K. and Singh, S. (2013). Introgression of productivity and other desirable traits from ricebean (Vigna umbellata) into black gram (Vigna mungo). Plant Breeding. 132(4): 401-406. 
  18. Smith, J., Chin, E., Shu, H., Smith, O., Wall, S., Senior, M. and Ziegle, J. (1997). An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theoretical and Applied Genetics. 95(2): 163-173. 
  19. Somta, P., Musch, W., Kongsamai, B., Chanprame, S., Nakasathien, S., Toojinda, T. and Srinives, P. (2008). New microsatellite markers isolated from mungbean [Vigna radiata (L.) Wilczek]. Molecular Ecology Resources. 8(5): 1155-1157. 
  20. Srimathy, M. and Jayamani, P. (2010). Cross species amplification of Adzuki Bean derived microsatellite markers in Asian Vigna species. Electronic Journal of Plant Breeding. 1(4): 1171-1179. 
  21. Srimathy, M., Sathya, M. and Jayamani, P. (2013). Cross species amplification of adzuki bean derived microsatellite markers and diversity analysis in blackgram [Vigna mungo (L.) Hepper]. Journal of Food Legumes. 26 (1): 15-19. 
  22. Tanksley, S., Young, N., Paterson, A. and Bonierbale, M. (1989). RFLP mapping in plant breeding: new tools for an old science. Nature Biotechnology. 7(3): 257. 
  23. Tian, J., Isemura, T., Kaga, A., Vaughan, D. and Tomooka, N. (2013). Genetic diversity of the rice bean (Vigna umbellata) genepool as assessed by SSR markers. Genome. 56(12): 717-727. 
  24. Tomooka, N., Naito, K., Kaga, A., Sakai, H., Isemura, T., Ogiso- Tanaka, E. and Takahashi, Y. (2014). Evolution, domestication and neo-domestication of the genus Vigna. Plant Genetic Resources. 12(S1): S168.
  25. Zhang, Y., Zhao, X. and Cheng, X. (2002). Screening and identification of bruchid resistant materials of mungbean. Proc. on Technology and Utilization of Mungbean in China. China Agriculture Press, Beijing, 116-118. 
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