Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Legume Research, volume 44 issue 8 (august 2021) : 977-985

Cultural, Morphological and Molecular Characterization of Fusarium Oxysporum f.sp. Ciceris Inciting Wilt of Chickpea

K. Venkataramanamma, B.V. Bhaskara Reddy, R. Sarada Jayalakshmi, V. Jayalakshmi, K.V. Hari Prasad, G. Mohan Naidu
1Department of Plant Pathology, Shri Venkateswara Agricultural College, Tirupati-517502, Andhra Pradesh, India.

  • Submitted13-06-2019|

  • Accepted04-09-2019|

  • First Online 09-11-2019|

  • doi 10.18805/LR-4178

Cite article:- Venkataramanamma K., Reddy Bhaskara B.V., Jayalakshmi Sarada R., Jayalakshmi V., Prasad Hari K.V., Naidu Mohan G. (2021). Cultural, Morphological and Molecular Characterization of Fusarium Oxysporum f.sp. Ciceris Inciting Wilt of Chickpea. Legume Research. 44(8): 977-985. doi: 10.18805/LR-4178.

ABSTRACT

Thirty two isolates of Fusarium oxysporum f.sp. ciceris (Foc) were collected from six districts of Andhra Pradesh (A.P) and three districts of  Telangana. Based on wilting % in pathogenicity test, the isolates were grouped into three categories. All the isolates differed in cultural characters like mycelium colour, margin, texture, pigmentation and morphological characters like macro conidia, micro conidia length, width and chlamydospores diameter. There was no correlation between cultural and morphological characters of Foc isolates except three isolates (Foc-6, Foc-12 and Foc-17). These three isolates have white mycelium, cream colour pigmentation, slow radial growth and highly pathogenic (100% wilt incidence) in nature. Sporulation ability and radial growth of all isolates were also measured.  All the isolates exhibited genetic variation in RAPD banding pattern and three major clusters (I, II and III) were found in the dendrogram. Maximum genetic similarity of 80.3% was observed between Foc-27 and Foc-28 isolates.

REFERENCES

  1. Anonymous, (2016-17). AICRP on chickpea project coordinators report, ICAR-CIAE, Bhopal, August 28th to 30th, 2017.
  2. Arvayo-Ortiz, R.M., Esqueda, M., Acedo-Felix, E., Sanchez, A. and Gutierrez, A. (2011). Morphological variability and races of Fusarium oxysporum f.sp. cicero associated with chickpea (Cicer arietinum L) crops. American Journal of Agricultual and Biological Sciences. 6(1): 114-121.
  3. Barhate, B.G., Dake, G.N., Game, B.C and Padule, D.N. (2006). Variability for virulence in Fusarium oxysporum f.sp. ciceri causing wilt of chickpea. Legume Research. 29(4): 308-310.
  4. Booth, C. (1971). The genus Fusarium Kew. Common wealth Mycological Institute, Kew (Surrey), England. 137.
  5. Burgess, L.W., Nelson, P.E. and Summerell, B.A. (1989). Variability and stability for morphological characters of Fusarium oxysporum isolated from soils in Australia. Mycologia. (81):818–822.
  6. Datta, J and Lal, N. (2012). Application of molecular markers for genetic discrimination of Fusarium wilt pathogen races affecting chickpea and pigeonpea in major regions of India. Cellular and Molecular Biology. 58 (1): 55-65.
  7. Dubey, S.C., Singh S. R. and Singh, B. (2010). Morphological and pathogenic variability of Indian isolates of Fusarium oxysporum f. sp.ciceris causing chickpea wilt. Archieves of Phytopathology and Plant Protection. 43 (2): 174-190.
  8. F.A.O (Food and Agricultural Organization) Reports. (2018). At www.faostat.org.
  9. Gordon, T. R. and Martyn, R.D. (1997). The evolutionary biology of Fusarium oxysporum. Annu Rev. Phytopathol. (35): 111–128. 
  10. Jalali, B.L and Hari Chand. (1992). In: Diseases of International Importance, Diseases of Cereals and Pulses. Vol. 1. (U.S. Singh, H.S. Chaube, J. Kumar and A.N. Mukhopadhyay, Eds.), Prentice Hall, Englewood Cliff, New Jersey, pp. 429-444.
  11. Kumar, A., Lal, H.C and Akhtar, J. (2012). Morphological and pathogenic Characteri-zation of Fusarium oxysporum f.sp. ciceri causing wilt of chickpea. Indian Phytopathology. 65(1): 64-66.
  12. Mandhare, V.K., Deshmukh, G.P., Patil, J.V., Kale, A.A and Havan, U.D. (2011). Morphological, pathological and molecular characterization of Fusarium oxysporum f. Sp. ciceri isolates from Maharashtra, India, Indonesia Journal of Agricultural Science. 12: 47-56.
  13. Molnar, A., Sulyok, L and Hornok, L. (1990). Parasexual recombination between vegetatively incompatible strains in Fusarium oxysporum. Mycol. Res. (94): 264. 393–398.
  14. Nath, N., Ahmed, A.U and Aminuzzaman, F.M. (2017). Morphological and physiological variation of Fusarium oxysporum f.sp. ciceri isolates causing wilt disease in chickpea. International Journal of Environment, Agriculture and Biotechnology. 2(1): 202-212.
  15. Nene, Y.L., Sheila, V.K and Sharma, S.B. (1984). A world list of chickpea (Cicer arietinum L.) and Pigeonpea (Cajanus cajan (L) Millsp.) pathogens. ICRISAT Pulse Pathology Progress Report. 32: pp.19.
  16. Nene, Y.L., Sheila, V.K. and Sharma, S.B. (1989). A world list of chickpea and pigeon pea Pathogens. In ICRISAT Legume Pathology Program Report, Patancheru, India, p. 7. 
  17. Powell, W., Morganate, M. andre, C., Hanafey, M., Vogel, J., Tingey and Rafalski, A. (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breed. (2): 225-238.
  18. Raeda and Broda (1985). DNA isolation procedure. Letters in Applied Microbiology. 30 (1): 53-57.
  19. Rangaswamy, G and Mahadevan, A. (1999). Diseases of Crop Plants in India (4th edition) Prentice Hall of India Pvt. Ltd., New Delhi, 607.
  20. Reddy, N. H and Dubey, S. C. (2006). Pathogenic and molecular characterization of Indian isolates of Fusarium oxysporum f. sp. ciceri causing chickpea wilt. Current science. 91(5): 661-666.
  21. Reddy, N.H and Dubey, S.C. (2007). Morphological characterization of Indian isolates of Fusarium oxysporum f. sp. ciceri causing chickpea wilt. Indian Phytopathology. 60 (3): 373-376.
  22. Rodrigues, A.A.C. and Menezes, M. (2005). Identification and pathogenic characterization of endophytic Fusarium species from cowpea seeds. Mycopathologia. 159: 79-85. 
  23. Silva, A.D.S., Oliviera E.J.D., Haddad, F., Jesus, O.N.D., Oliveira S.A.S.D and Costa, M.A. P.D.C. (2013). Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers. Sci. Agric. 70 (2): 108-115.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)