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Current IssueEditorial BoardOnline First Articles
Legume Research
Print ISSN: 0250-5371
Online ISSN: 0976-0571
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Chief Editor:
J. S. Sandhu
Vice Chancellor, SKN Agriculture, University, Jobner, VC, NDUAT, Faizabad, Deputy Director General (Crop Science), Indian Council of Agricultural Research (ICAR), New Delhi
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Research Article

Legume Research, volume 44 issue 7 (july 2021) : 854-858

Molecular characterization of Candidatus Phytoplasma aurantifolia isolate infecting chickpea (Cicer arietinum) in Dharwad, Karnataka

Gurupada Balol, C. Channakeshava, M.S. Patil
1All India Co-ordinated Research Project on MULLARP, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad-580 005, Karnataka, India.

  • Submitted16-04-2019|

  • Accepted27-06-2019|

  • First Online 03-12-2019|

  • doi 10.18805/LR-4156

Cite article:- Balol Gurupada, Channakeshava C., Patil M.S. (2021). Molecular characterization of Candidatus Phytoplasma aurantifolia isolate infecting chickpea (Cicer arietinum) in Dharwad, Karnataka. Legume Research. 44(7): 854-858. doi: 10.18805/LR-4156.

ABSTRACT

Chickpea plants showing phytoplasma symptoms were observed in the research plots at University of Agricultural Sciences, Dharwad, Karnataka, India. The symptoms included phyllody, pale green leaves, bushy appearance and excessive axillary proliferation. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by nested PCR with primers P1/P7 and R16F2n/R16R2 and 1,800 bp and 1,200 bp size products were amplified in first round PCR and nested-PCR respectively. The PCR product was sequenced and compared with the reference phytoplasma sequences collected from the database (NCBI). 16S rDNA sequences of Dharwad chickpea phytoplasma shared the highest nucleotide identity of (>98%) with Periwinkle phyllody16SrII-E (EU096500). This study indicated the association of ‘Candidatus Phytoplasma aurantifolia’ the 16SrII-E group infecting chickpea from Northern Karnataka.

REFERENCES

  1. Akhtar, K.P., Shah, T.M., Atta, B.M., Dickison, M., Jamil, F.F., and Haq, M. A. (2008). Natural occurrence of phytoplasma associated with chickpea phyllody disease in Pakistan-a new record. Plant Pathology. 57: 771-773. 
  2. Al-Saady, N.A., Al-Subhi, A.M., Al-Nabhani, A., Khan, A.J. (2006).First report of a group 16SrII phytoplasma infecting chickpea in Oman. Plant Disease. 90: 734.
  3. Anonimous, (2018). Pulses Revolution - From Food to Nutritional Security. Min. of Agri. & FW (DAC&FW), GOI. pp. 105.
  4. Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bulletin. 19:11-15.
  5. Ghanekar, A.M., Manohar, S.K., Reddy, S.V. and Nene, Y.L. (1988). Association of a mycoplasma-like organism with chickpea phyllody. Indian Phytopathology. 41: 462-464.
  6. Harrison, N.A., Womack, M. and Carpio, M.L. (2002). Detection and characterization of a lethal yellowing (16SrIV) group phytoplasma in Canary Island date palms affected by lethal decline in Texas. Plant Disease. 86: 676-681.
  7. Lee, I.M. and Davis, R.E. (1992). Mycoplasmas which infectplants and insects. In: Mycoplasmas: Molecular biology and pathogenesis. [J. Manil off, R. N. McElhaney, L. R. Finch, & J. B. Baseman (Eds.)], Washington, DC: American Society for Microbiology. pp. 379-390.
  8. Lee, I.M., Gundersen-Rindal, D.E., Davis, R.E., Botter, K.D. and Seemuller, E. (2004). Candidatus phytoplasma asteries, a novel phytoplasma taxon associated with aster yellows and related diseases. International Journal of Systematic and Evolutionary Microbiology. 54: 1037-1048.
  9. Marcone, C., Lee, I.M., Davis, R.E., Ragozzino, A. and Seemuller, E. (2000). Classification of aster yellows-group phytoplasmas based on combined analysis of rRNA and tuf gene sequences. International Journal of Systematic and Environmental Microbiology. 50: 1703-1713.
  10. Montano, H.G., Davis, R.E., Daily, E.L., Hogenhout, S., Pimenlet, J.P. and Brioso, P.S.T. (2001). ‘Candidatus phytoplasma brasiliense’, a new phytoplasma taxon associated with hibiscus witches’ broom disease. International Journal of Systematic and Environmental Microbiology. 51: 1109-1118.
  11. Mourya, O. and Kumar, H., (2018), Growth of chickpea production in India, Journal of Pharmacognosy and Photochemistry. 7(5) 1175-1177.
  12. Pallavi, M.S., Ramappa, H.K., Shankarappa, K.S., Rangaswamy, K.T., Wickramaarachchi, W. A.R.T. and Maruthi, M.N. (2012). Detection and molecular characterization of phytoplasma associated with chickpea phyllody disease in south India. Phytoparasitica. 40:279-286.
  13. Ramappa, H.K., Chandrasekar, K., Patil, C.S.P. and Pallavi, M.S. (2008). Occurrence of Ascochyta blight and phyllody diseases of chickpea in southern Karnataka. In: National Seminar on Advances in plant Pathology for Sustainable Agriculture Coimbatore, India, pp. 35-36.
  14. Saady, N.A., Subhi, A.M., Nabhani, A. and Khan, A.J. (2006). First report of agroup 16SrII phytoplasma infecting chickpea in Oman. Plant Disease. 90:734.
  15. Samuitiene, M. and Navalinskiene, M. (2006). Molecular detection and characterization of phytoplasma infecting Celosia argentea plants in Lithuania. Agronomy Research. 4:345-348.
  16. Saqib, M., Bayliss, K.L. and Jones, M.G.K. (2006). Identificationof sweet potato little leaf phytoplasma associated with Vigna unguiculata var. sesquipedalis and Lycopersicon esculentum. Australasian Plant Pathology. 35: 293-296.
  17. Sertkaya, G., Martini, M., Ermacora, P., Musetti, R. and Osler, R., (2007). Detection and molecular characterization of phytoplasmas infecting sesame and solanaceous crops in Turkey. Bulletin of Insectology. 60(2): 141-142.
  18. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei and M., Kumar, S., (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony, Methods of Molecular Biolology and Evolution. 28: 2731-2739.
  19. Vijay Kumar Naik, D.B.V., Bhaskara Reddy, J., Sailaja Rani,o]/ R., Sarada Jayalakshmi Devi and Hari Prasad, K.V. (2018). Natural occurrence of phytoplasma associated with chickpea phyllody in Andhra Pradesh, India. International Journal of Current Microbiology and Applied Sciences. 7(07): 171-176. 
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