Published In
Legume Research
Article Metrics

0
Views
0
Citations
Reviewed By
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
Follow us
Research Article
volume 44 issue 7 (july 2021) : 854-858, Doi: 10.18805/LR-4156
Molecular characterization of Candidatus Phytoplasma aurantifolia isolate infecting chickpea (Cicer arietinum) in Dharwad, Karnataka
1All India Co-ordinated Research Project on MULLARP, Main Agricultural Research Station, University of Agricultural Sciences, Dharwad-580 005, Karnataka, India.
Submitted16-04-2019|
Accepted27-06-2019|
First Online 03-12-2019|
doi 10.18805/LR-4156
Cite article:- Balol Gurupada, Channakeshava C., Patil M.S. (2021). Molecular characterization of Candidatus Phytoplasma aurantifolia isolate infecting chickpea (Cicer arietinum) in Dharwad, Karnataka. Legume Research. 44(7): 854-858. doi: 10.18805/LR-4156.
ABSTRACT
Chickpea plants showing phytoplasma symptoms were observed in the research plots at University of Agricultural Sciences, Dharwad, Karnataka, India. The symptoms included phyllody, pale green leaves, bushy appearance and excessive axillary proliferation. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by nested PCR with primers P1/P7 and R16F2n/R16R2 and 1,800 bp and 1,200 bp size products were amplified in first round PCR and nested-PCR respectively. The PCR product was sequenced and compared with the reference phytoplasma sequences collected from the database (NCBI). 16S rDNA sequences of Dharwad chickpea phytoplasma shared the highest nucleotide identity of (>98%) with Periwinkle phyllody16SrII-E (EU096500). This study indicated the association of ‘Candidatus Phytoplasma aurantifolia’ the 16SrII-E group infecting chickpea from Northern Karnataka.
REFERENCES
- Akhtar, K.P., Shah, T.M., Atta, B.M., Dickison, M., Jamil, F.F., and Haq, M. A. (2008). Natural occurrence of phytoplasma associated with chickpea phyllody disease in Pakistan-a new record. Plant Pathology. 57: 771-773.
- Al-Saady, N.A., Al-Subhi, A.M., Al-Nabhani, A., Khan, A.J. (2006).First report of a group 16SrII phytoplasma infecting chickpea in Oman. Plant Disease. 90: 734.
- Anonimous, (2018). Pulses Revolution - From Food to Nutritional Security. Min. of Agri. & FW (DAC&FW), GOI. pp. 105.
- Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bulletin. 19:11-15.
- Ghanekar, A.M., Manohar, S.K., Reddy, S.V. and Nene, Y.L. (1988). Association of a mycoplasma-like organism with chickpea phyllody. Indian Phytopathology. 41: 462-464.
- Harrison, N.A., Womack, M. and Carpio, M.L. (2002). Detection and characterization of a lethal yellowing (16SrIV) group phytoplasma in Canary Island date palms affected by lethal decline in Texas. Plant Disease. 86: 676-681.
- Lee, I.M. and Davis, R.E. (1992). Mycoplasmas which infectplants and insects. In: Mycoplasmas: Molecular biology and pathogenesis. [J. Manil off, R. N. McElhaney, L. R. Finch, & J. B. Baseman (Eds.)], Washington, DC: American Society for Microbiology. pp. 379-390.
- Lee, I.M., Gundersen-Rindal, D.E., Davis, R.E., Botter, K.D. and Seemuller, E. (2004). Candidatus phytoplasma asteries, a novel phytoplasma taxon associated with aster yellows and related diseases. International Journal of Systematic and Evolutionary Microbiology. 54: 1037-1048.
- Marcone, C., Lee, I.M., Davis, R.E., Ragozzino, A. and Seemuller, E. (2000). Classification of aster yellows-group phytoplasmas based on combined analysis of rRNA and tuf gene sequences. International Journal of Systematic and Environmental Microbiology. 50: 1703-1713.
- Montano, H.G., Davis, R.E., Daily, E.L., Hogenhout, S., Pimenlet, J.P. and Brioso, P.S.T. (2001). ‘Candidatus phytoplasma brasiliense’, a new phytoplasma taxon associated with hibiscus witches’ broom disease. International Journal of Systematic and Environmental Microbiology. 51: 1109-1118.
- Mourya, O. and Kumar, H., (2018), Growth of chickpea production in India, Journal of Pharmacognosy and Photochemistry. 7(5) 1175-1177.
- Pallavi, M.S., Ramappa, H.K., Shankarappa, K.S., Rangaswamy, K.T., Wickramaarachchi, W. A.R.T. and Maruthi, M.N. (2012). Detection and molecular characterization of phytoplasma associated with chickpea phyllody disease in south India. Phytoparasitica. 40:279-286.
- Ramappa, H.K., Chandrasekar, K., Patil, C.S.P. and Pallavi, M.S. (2008). Occurrence of Ascochyta blight and phyllody diseases of chickpea in southern Karnataka. In: National Seminar on Advances in plant Pathology for Sustainable Agriculture Coimbatore, India, pp. 35-36.
- Saady, N.A., Subhi, A.M., Nabhani, A. and Khan, A.J. (2006). First report of agroup 16SrII phytoplasma infecting chickpea in Oman. Plant Disease. 90:734.
- Samuitiene, M. and Navalinskiene, M. (2006). Molecular detection and characterization of phytoplasma infecting Celosia argentea plants in Lithuania. Agronomy Research. 4:345-348.
- Saqib, M., Bayliss, K.L. and Jones, M.G.K. (2006). Identificationof sweet potato little leaf phytoplasma associated with Vigna unguiculata var. sesquipedalis and Lycopersicon esculentum. Australasian Plant Pathology. 35: 293-296.
- Sertkaya, G., Martini, M., Ermacora, P., Musetti, R. and Osler, R., (2007). Detection and molecular characterization of phytoplasmas infecting sesame and solanaceous crops in Turkey. Bulletin of Insectology. 60(2): 141-142.
- Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei and M., Kumar, S., (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony, Methods of Molecular Biolology and Evolution. 28: 2731-2739.
- Vijay Kumar Naik, D.B.V., Bhaskara Reddy, J., Sailaja Rani,o]/ R., Sarada Jayalakshmi Devi and Hari Prasad, K.V. (2018). Natural occurrence of phytoplasma associated with chickpea phyllody in Andhra Pradesh, India. International Journal of Current Microbiology and Applied Sciences. 7(07): 171-176.
Disclaimer :
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Copyright :
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
Follow us
Published In
Legume Research