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Research Article

volume 42 issue 2 (april 2019) : 277-281,   Doi: 10.18805/LR-3843

Pathogenic and morphological variability among Fusarium oxysporum f. sp. ciceri isolates causing wilt in chickpea

H
H.R. Chaithra
H
H. Manjunatha
M
M. Saifulla
P
Pagala Deepthi
1Department of Plant Pathology, Gandhi Krishi Vignan Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India.

  • Submitted30-01-2017|

  • Accepted06-04-2018|

  • First Online 20-08-2018|

  • doi 10.18805/LR-3843

Cite article:- Chaithra H.R., Manjunatha H., Saifulla M., Deepthi Pagala (2018). Pathogenic and morphological variability among Fusarium oxysporum f. sp. ciceri isolates causing wilt in chickpea. Legume Research. 42(2): 277-281. doi: 10.18805/LR-3843.

ABSTRACT

Twelve Fusarium oxysporum f. sp. ciceri isolates were isolated from chickpea infected samples collected across different states of India. Pathogenic variability of twelve isolates was observed on seven chickpea cultivars viz., JG 62, Annigeri-1, Chaffa, CPS-1, K 850, L 550 and DCP 92-3. Among the twelve isolates APFOC-1 showed lowest wilt incidence 71.43 per cent and the isolate MPFOC-9 showed 100 per cent wilt incidence in all cultivars. The variability in morphological characters like size, shape, color of conidia and size, shape, color of chlamydospore were observed in all the isolates.  The size of macroconidia varied from 9.23X2.53 ìm (WBFOC-12) to 19.3X5.52 ìm (MHFOC-8) with 2-4 septation. The size of microconidia varied from 4.4X2.93 ìm (MPFOC-9) to 8.26X4.2 ìm (MHFOC-8) with 0-1 septation. Macroconidia were sickle shaped and blunt ends with hyaline color. Microconidia were round to oval with hyaline color. Size of chlamydospore varied from 2.67 (UPFOC-11) to 8.24 (JKFOC-5). Chlamydospores did not show much variation with respect to shape and color. Based on the total number of spores observed per microscopic field, the twelve isolates categorized into four groups viz., poor sporulants, moderate sporulants, good sporulants and very good sporulants.

REFERENCES

  1. Booth, C. (1971). The genus Fusarium, Commonwealth Mycological Institute, Kew (Surry), England, pp.137.
  2. Butler, E. J. (1918). Fungi and Diseases in Plants. Thacker Spink and Co., Calcutta India, pp. 547.
  3. Chube, H. S. (1968). Combating diseases of Arhar and Gram. Indian Farmers Digest, 1: 26.
  4. Dubey, S.C. . Singh and Birendra. (2004). Reaction of chickpea genotypes against Fusarium oxysporum f. sp. ciceri causing vascular wilt. Indian Phytopath. 52(2): 233.
  5. Gupta, Om., Khare, M.N. and Kothathane, S.R.(1986). Variability among six isolates of Fusarium oxysporum f. sp. ciceri. causing vascular wilt of chickpea. Indian Phytopath. 39: 279- 281.
  6. Haware, M.P. and Nene Y.L. (1982). Races of Fusarium oxysporum f.sp. ciceri. Pl. Dis., 66: 809-810.
  7. Imran Khan, H. S. (2004). Studies on Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceri. M.Sc. Thesis.Univ. Agri. Sci., Bangalore. pp 1-100.
  8. Jalali, B. L. and Harichand.(1992). Chickpea wilt. In: Disease of Cereals Pulses,Vol.1.pp.56. 
  9. Jimenez-Diaz, R.M., Alcala-Jimenez A.R., Hervas A. and Trapero-Casas J.L. (1993). Pathogenic variability and host resistance in the Fusarium oxysporum f. sp. ciceris/Cicer arietinum pathosystem. Proc. 3rd Eur. Semin. Fusarium mycotoxins, taxonomy, pathogenicity and host resistance. Radzikov (Poland): Plant Breeding and Acclimatization Institute. pp. 87-94.
  10. Kadam, N. and Mane, S. S. (2012). Molecular characterization of different isolates of Fusarium oxysporum f. sp. ciceri causing chickpea wilt in Maharashtra using RAPD. J. Pl. Dis. Sci. 7 (2): 206- 209.
  11. Ladizinsky, G. (1975). A new Cicer from Turkey. The Royal Botanic Garden Edinburgh, 34: 201-202.
  12. Mandhare, V. K., Deshmukha, G. P., Patilb, J. V., Kalec, A. A. and Chavand, U. D. (2011). Morphological, pathogenic and molecular characterization of Fusarium oxysporum f. sp. ciceri isolates. Indonesian J. Agril. Sci., 12(2):47-56.
  13. Paul, Joginder, Gill, T.S. and Singh, R.S. (2001). Variability among the isolates of Fusarium oxysporum f. sp. ciceri from chickpea roots and rhizophore. Pl. Dis. Res., 16: 116-118.
  14. Singh, N. P. (2013). Chickpea Project co-ordinator report, AICRP on chickpea, Patancheru, Hyderabad, pp 27. 
  15. Srivastava, A., Singh, S. N. and Agrawal, S. C., (2004). Studies on prevalence and identification of new races of Fusarium oxysporum f. sp. ciceri, incitant of chickpea wilt from Madhya Pradesh. India. J. Pl. Pathol. 22 (1 & 2): 88-90.
  16. Van Der Maesen, L. J. G. (1972). A monograph of the genus with special reference to the chickpea (Cicer arietinum. L), its ecology and cultivation. Commun. Agric., pp. 208-216. 
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    Research Article

    volume 42 issue 2 (april 2019) : 277-281,   Doi: 10.18805/LR-3843

    Pathogenic and morphological variability among Fusarium oxysporum f. sp. ciceri isolates causing wilt in chickpea

    H
    H.R. Chaithra
    H
    H. Manjunatha
    M
    M. Saifulla
    P
    Pagala Deepthi
    1Department of Plant Pathology, Gandhi Krishi Vignan Kendra, University of Agricultural Sciences, Bengaluru-560 065, Karnataka, India.

    • Submitted30-01-2017|

    • Accepted06-04-2018|

    • First Online 20-08-2018|

    • doi 10.18805/LR-3843

    Cite article:- Chaithra H.R., Manjunatha H., Saifulla M., Deepthi Pagala (2018). Pathogenic and morphological variability among Fusarium oxysporum f. sp. ciceri isolates causing wilt in chickpea. Legume Research. 42(2): 277-281. doi: 10.18805/LR-3843.

    ABSTRACT

    Twelve Fusarium oxysporum f. sp. ciceri isolates were isolated from chickpea infected samples collected across different states of India. Pathogenic variability of twelve isolates was observed on seven chickpea cultivars viz., JG 62, Annigeri-1, Chaffa, CPS-1, K 850, L 550 and DCP 92-3. Among the twelve isolates APFOC-1 showed lowest wilt incidence 71.43 per cent and the isolate MPFOC-9 showed 100 per cent wilt incidence in all cultivars. The variability in morphological characters like size, shape, color of conidia and size, shape, color of chlamydospore were observed in all the isolates.  The size of macroconidia varied from 9.23X2.53 ìm (WBFOC-12) to 19.3X5.52 ìm (MHFOC-8) with 2-4 septation. The size of microconidia varied from 4.4X2.93 ìm (MPFOC-9) to 8.26X4.2 ìm (MHFOC-8) with 0-1 septation. Macroconidia were sickle shaped and blunt ends with hyaline color. Microconidia were round to oval with hyaline color. Size of chlamydospore varied from 2.67 (UPFOC-11) to 8.24 (JKFOC-5). Chlamydospores did not show much variation with respect to shape and color. Based on the total number of spores observed per microscopic field, the twelve isolates categorized into four groups viz., poor sporulants, moderate sporulants, good sporulants and very good sporulants.

    REFERENCES

    1. Booth, C. (1971). The genus Fusarium, Commonwealth Mycological Institute, Kew (Surry), England, pp.137.
    2. Butler, E. J. (1918). Fungi and Diseases in Plants. Thacker Spink and Co., Calcutta India, pp. 547.
    3. Chube, H. S. (1968). Combating diseases of Arhar and Gram. Indian Farmers Digest, 1: 26.
    4. Dubey, S.C. . Singh and Birendra. (2004). Reaction of chickpea genotypes against Fusarium oxysporum f. sp. ciceri causing vascular wilt. Indian Phytopath. 52(2): 233.
    5. Gupta, Om., Khare, M.N. and Kothathane, S.R.(1986). Variability among six isolates of Fusarium oxysporum f. sp. ciceri. causing vascular wilt of chickpea. Indian Phytopath. 39: 279- 281.
    6. Haware, M.P. and Nene Y.L. (1982). Races of Fusarium oxysporum f.sp. ciceri. Pl. Dis., 66: 809-810.
    7. Imran Khan, H. S. (2004). Studies on Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceri. M.Sc. Thesis.Univ. Agri. Sci., Bangalore. pp 1-100.
    8. Jalali, B. L. and Harichand.(1992). Chickpea wilt. In: Disease of Cereals Pulses,Vol.1.pp.56. 
    9. Jimenez-Diaz, R.M., Alcala-Jimenez A.R., Hervas A. and Trapero-Casas J.L. (1993). Pathogenic variability and host resistance in the Fusarium oxysporum f. sp. ciceris/Cicer arietinum pathosystem. Proc. 3rd Eur. Semin. Fusarium mycotoxins, taxonomy, pathogenicity and host resistance. Radzikov (Poland): Plant Breeding and Acclimatization Institute. pp. 87-94.
    10. Kadam, N. and Mane, S. S. (2012). Molecular characterization of different isolates of Fusarium oxysporum f. sp. ciceri causing chickpea wilt in Maharashtra using RAPD. J. Pl. Dis. Sci. 7 (2): 206- 209.
    11. Ladizinsky, G. (1975). A new Cicer from Turkey. The Royal Botanic Garden Edinburgh, 34: 201-202.
    12. Mandhare, V. K., Deshmukha, G. P., Patilb, J. V., Kalec, A. A. and Chavand, U. D. (2011). Morphological, pathogenic and molecular characterization of Fusarium oxysporum f. sp. ciceri isolates. Indonesian J. Agril. Sci., 12(2):47-56.
    13. Paul, Joginder, Gill, T.S. and Singh, R.S. (2001). Variability among the isolates of Fusarium oxysporum f. sp. ciceri from chickpea roots and rhizophore. Pl. Dis. Res., 16: 116-118.
    14. Singh, N. P. (2013). Chickpea Project co-ordinator report, AICRP on chickpea, Patancheru, Hyderabad, pp 27. 
    15. Srivastava, A., Singh, S. N. and Agrawal, S. C., (2004). Studies on prevalence and identification of new races of Fusarium oxysporum f. sp. ciceri, incitant of chickpea wilt from Madhya Pradesh. India. J. Pl. Pathol. 22 (1 & 2): 88-90.
    16. Van Der Maesen, L. J. G. (1972). A monograph of the genus with special reference to the chickpea (Cicer arietinum. L), its ecology and cultivation. Commun. Agric., pp. 208-216. 
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