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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 41 issue 2 (april 2018) : 196-201,   Doi: 10.18805/LR-3709

Diversity analysis of pea genotypes using RAPD markers

B
Bharti Thakur
S
Susheel Sharma
I
Isha Sharma
P
Prachi Sharma
D
Deepika
S
Sajad Majeed Zargar
1Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu-180 009, Jammu and Kashmir, India.

  • Submitted23-03-2016|

  • Accepted11-03-2017|

  • First Online 20-01-2018|

  • doi 10.18805/LR-3709

Cite article:- Thakur Bharti, Sharma Susheel, Sharma Isha, Sharma Prachi, Deepika, Zargar Majeed Sajad (2018). Diversity analysis of pea genotypes using RAPD markers. Legume Research. 41(2): 196-201. doi: 10.18805/LR-3709.

ABSTRACT

Genetic diversity among 54 genotypes of field and garden pea of India were studied using 30 RAPD primers. A total of 168 amplicons were scored, of which 154 were polymorphic revealing 89.3% of polymophism. Out of 30 RAPD primers tested, 22 primers produced clear and reproducible bands. The number of products generated by these primers ranged from 04 to 14 with primer OPU-17 giving maximum (14) and primer OPC-08 giving minimum number of amplicons (04). Genetic similarity estimates based on the binomial data using Jaccard’s coefficient ranged from 0.34 (Kaza-2/IC-218991) to 0.89 (IC-209118/IC-209123) with an average similarity index of 0.54 exhibiting considerable diversity among the pea genotypes studied. The dendrogram showed clear pattern of clustering according to the source of germplasm. This study indicated that Himalayan region germplasm exhibited higher diversity. 

REFERENCES

  1. Baranger A., Aubert G., Arnau G., Laine A. L., Deniot G. and Potier J. (2004). Genetic diversity within Pisum sativum using protein and PCR-based markers. Theoretical and Applied Genetics, 108:1309-1321.
  2. Choudhury R.P., Tanveer H. and Dixit G.P. (2007). Identification and detection of genetic relatedness among important varieties of pea grown in India. Genetica, 130: 183-191.
  3. Gaafar, R. M. and Saker, M. M. (2006). Monitoring of cultivars Identity and Genetic stability in strawberry varieties grown in Egypt. World Journal of Agricultural Sciences, 2(1): 29-36.
  4. Heath M. C. and Hebblethwaite P. D. (1985). Agronomic problems associated with the pea crop. The pea Crop: a basis for improvement. Butterworths, London. pp 19-29.
  5. Kapila K., Naryal S. and Dhiman K.C. (2011). Analysis of Genetic diversity among garden and field pea genotypes of Higher Indian Himalayas. Journal of Plant Biochemistry and Biotechnology, 21(2):286–291.
  6. Kumar, R., Kumar, M., Dogra, R.K. and Bharat, N.K. (2015). Variability and character association studies in garden pea (Pisum sativum var. hortense L.) during winter season at mid hills of Himachal Pradesh. Legume Research, 38 (2): 164-168.
  7. Nicese, F. P., Hormaza, J. I. and McGranhan, G. H. (1998). Molecular characterization and genetic relatedness among walnut genotypes based on RAPD markers. Euphytica. 101: 199-206.
  8. Prevost A. and Wilkinson M. J. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98: 107-112.
  9. Rohlf F.J. (1998). NTSYSpc: numerical taxonomy and multivariate analysis system version 2.02g. Exeter Software, Setauket, NY.
  10. Roldan-Ruiz I., Dendauw J. and Depicker A. (2000). AFLP reveals high polymorphic rates in ryegrasses. Molecular Breeding, 6: 125-134.
  11. Santalla M., Amurrio J. M. and De Ron A. M. (2001). Food and feed potencial breeding value of green, dry and vegetal pea germplasm. Canadian Journal of Plant Science, 81: 601–610.
  12. Singh, J., Dhall, R.K. and Aulakh, P.S. (2011). Molecular characterization of biodiversity on vegetables- A review. Agricultural Reviews, 32(3):193-201.
  13. Simioniuc D., Uptmoor R., Friedt W. and Ordon F. (2002). Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs. Plant Breeding, 121: 429–435.
  14. Smýkal P., Hýbl M., Corander J., Jarkovský J., Flavell A. J. and Griga M. (2008). Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis. Theoretical and Applied Genetics, 117: 413–424.
  15. Tar’an B., Zhang C., Warkentin T. and Tullu A. (2005). Genetic diversity among varieties and wild species accessions of pea (Pisum sativum L.) based on molecular markers, and morphological and physiological characters. Genome, 48: 257-272.
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    Research Article

    volume 41 issue 2 (april 2018) : 196-201,   Doi: 10.18805/LR-3709

    Diversity analysis of pea genotypes using RAPD markers

    B
    Bharti Thakur
    S
    Susheel Sharma
    I
    Isha Sharma
    P
    Prachi Sharma
    D
    Deepika
    S
    Sajad Majeed Zargar
    1Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu-180 009, Jammu and Kashmir, India.

    • Submitted23-03-2016|

    • Accepted11-03-2017|

    • First Online 20-01-2018|

    • doi 10.18805/LR-3709

    Cite article:- Thakur Bharti, Sharma Susheel, Sharma Isha, Sharma Prachi, Deepika, Zargar Majeed Sajad (2018). Diversity analysis of pea genotypes using RAPD markers. Legume Research. 41(2): 196-201. doi: 10.18805/LR-3709.

    ABSTRACT

    Genetic diversity among 54 genotypes of field and garden pea of India were studied using 30 RAPD primers. A total of 168 amplicons were scored, of which 154 were polymorphic revealing 89.3% of polymophism. Out of 30 RAPD primers tested, 22 primers produced clear and reproducible bands. The number of products generated by these primers ranged from 04 to 14 with primer OPU-17 giving maximum (14) and primer OPC-08 giving minimum number of amplicons (04). Genetic similarity estimates based on the binomial data using Jaccard’s coefficient ranged from 0.34 (Kaza-2/IC-218991) to 0.89 (IC-209118/IC-209123) with an average similarity index of 0.54 exhibiting considerable diversity among the pea genotypes studied. The dendrogram showed clear pattern of clustering according to the source of germplasm. This study indicated that Himalayan region germplasm exhibited higher diversity. 

    REFERENCES

    1. Baranger A., Aubert G., Arnau G., Laine A. L., Deniot G. and Potier J. (2004). Genetic diversity within Pisum sativum using protein and PCR-based markers. Theoretical and Applied Genetics, 108:1309-1321.
    2. Choudhury R.P., Tanveer H. and Dixit G.P. (2007). Identification and detection of genetic relatedness among important varieties of pea grown in India. Genetica, 130: 183-191.
    3. Gaafar, R. M. and Saker, M. M. (2006). Monitoring of cultivars Identity and Genetic stability in strawberry varieties grown in Egypt. World Journal of Agricultural Sciences, 2(1): 29-36.
    4. Heath M. C. and Hebblethwaite P. D. (1985). Agronomic problems associated with the pea crop. The pea Crop: a basis for improvement. Butterworths, London. pp 19-29.
    5. Kapila K., Naryal S. and Dhiman K.C. (2011). Analysis of Genetic diversity among garden and field pea genotypes of Higher Indian Himalayas. Journal of Plant Biochemistry and Biotechnology, 21(2):286–291.
    6. Kumar, R., Kumar, M., Dogra, R.K. and Bharat, N.K. (2015). Variability and character association studies in garden pea (Pisum sativum var. hortense L.) during winter season at mid hills of Himachal Pradesh. Legume Research, 38 (2): 164-168.
    7. Nicese, F. P., Hormaza, J. I. and McGranhan, G. H. (1998). Molecular characterization and genetic relatedness among walnut genotypes based on RAPD markers. Euphytica. 101: 199-206.
    8. Prevost A. and Wilkinson M. J. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98: 107-112.
    9. Rohlf F.J. (1998). NTSYSpc: numerical taxonomy and multivariate analysis system version 2.02g. Exeter Software, Setauket, NY.
    10. Roldan-Ruiz I., Dendauw J. and Depicker A. (2000). AFLP reveals high polymorphic rates in ryegrasses. Molecular Breeding, 6: 125-134.
    11. Santalla M., Amurrio J. M. and De Ron A. M. (2001). Food and feed potencial breeding value of green, dry and vegetal pea germplasm. Canadian Journal of Plant Science, 81: 601–610.
    12. Singh, J., Dhall, R.K. and Aulakh, P.S. (2011). Molecular characterization of biodiversity on vegetables- A review. Agricultural Reviews, 32(3):193-201.
    13. Simioniuc D., Uptmoor R., Friedt W. and Ordon F. (2002). Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs. Plant Breeding, 121: 429–435.
    14. Smýkal P., Hýbl M., Corander J., Jarkovský J., Flavell A. J. and Griga M. (2008). Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis. Theoretical and Applied Genetics, 117: 413–424.
    15. Tar’an B., Zhang C., Warkentin T. and Tullu A. (2005). Genetic diversity among varieties and wild species accessions of pea (Pisum sativum L.) based on molecular markers, and morphological and physiological characters. Genome, 48: 257-272.
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