Loading...

Incidence of bacterial blight pathogen Pseudomonas syringae pv. pisi in pea seeds grown in Rajasthan, India

DOI: 10.18805/lr.v0iOF.8606    | Article Id: LR-3486 | Page : 1034-1037
Citation :- Incidence of bacterial blight pathogen Pseudomonas syringae pv. pisiin pea seeds grown in Rajasthan, India .Legume Research.2016.(39):1034-1037

Ashwani Kumar Verma1, Pooja Arora2 and Kailash Agrawal

ashwani14286@gmail.com
Address :

Department of Botany, University of  Rajasthan, Jaipur-302 001, India

Submitted Date : 21-04-2015
Accepted Date : 4-07-2015

Abstract

Seeds of pea (Pisum sativum L.) originated from various districts of Rajasthan, India were collected and tested from 2010 to 2014 for the presence of seed borne bacterial pathogen namely Pseudomonas syringae pv. pisi causing bacterial blight of pea. The pathogen was identified using morphological, cultural, biochemical, pathogenicity and host tests followed by 16S rRNA based molecular characterization. The results showed that these strains belonged to Pseudomonas syringae pv. pisi. The partial 16S rRNA sequences obtained were deposited in the database of Genebank, NCBI (Acc. No. KF251036). The incidence of the pathogen was recorded 3.5 to 91.5% in pea seed samples collected from 25 districts of Rajasthan. The results showed that highest (91.5%) incidence was recorded in Tonk, followed by Jaipur (87%) and Dausa (85.5%) while lowest incidence recorded in pea seed samples from Ajmer (3.5%).

Keywords

Incidence Molecular characterization Pea seeds Pseudomonas syringae pv. pisi.

References

  1. Agrawal K. and Jain, R. (2011). Seed borne bacterial diseases of cluster bean and their management. J. Indian Bot. Soc. suppl. vol.:301-308.
  2. Anonymous, (1985). International rules for seed testing association (ISTA). Seed Sci. Technol., 4: 3-49.
  3. Anonymous, (2014). Agricultural Statistics. http:// www.krishi.rajasthan.gov.in /Departments/Agriculture/    mainhindi.asp?t=statics_top.htm&p=statics_index_new.htm.
  4. Bradbury, J.F. (1986). Guide to Plant Pathogenic Bacteria. CAB International Mycological Institute, New York, USA, pp: 332.
  5. Chakravarthy, C.N., Krishnappa, M. and Thippeswamy, B. (2004). Investigation on bacterila blight (Xanthomonas axonopodis pv. cymopsidis) of cluster bean [Cymopsis tetragonoloba (L.) Taub.] and in vitro control. Indian J. Plant Pathol., 22: 68-74.
  6. Cirvilleri, G., Scuderi, G., Catara, V. and Scortichini, M. (2007). Typing of Pseudomonas syringae pv. pisi strains by fluorescent AFLP fingerprinting. J. Plant Pathol., 89: 421-425.
  7. Fahy, P.C. and Persley, G.J. (1983). Plant Bacterial Diseases: A Diagnostic Guide. Academic Press, Sydney, London, pp: 393.
  8. Garcia-Martinez, J., Bescos, I., Rodriguez-Sala, J.J. and Rodriguez-Valera, F. (2001). RISSC: A novel database for ribosomal 16S-23S RNA genes spacer regions. Nucleic Acids Res., 29: 178-180.
  9. Gitaitis, R. and Walcott, R. (2007). The epidemiology and management of seedborne bacterial diseases. Ann. Rev. Phytopathol., 45: 371-397.
  10. Han, H. and Baik, B.K. (2008). Antioxidant activity and phenolic content of lentils (Lens culinaris), chickpeas (Cicer arietinum L.), peas (Pisum sativum L.) and soybeans (Glycine max) and their quantitative changes during processing. Int. J. Food Sci. Technol., 43: 1971-1978.
  11. Hildebrand, D.C. and Schroth, M.N. (1972). Identification of fluorescent pseudomonas. Proceedings of the 3rd International Conference on Plant Pathogenic Bacteria, April 14-21, 1971, Wageningen, The Netherlands, pp. 281-287.
  12. Hollaway, G.J., Bretag, T.W. and Price, T.V. (2007). The epidemiology and management of bacterial blight (Pseudomonas syringae pv. pisi) of field pea (Pisum sativum) in Australia: A review. Aust. J. Agric. Res., 58: 1086-1099.
  13. Jain, R. and Agrawal, K. (2011). Bio-efficacy of plant extracts against Pseudomonas syringae pv. syringae causing leaf spot of Cluster bean. Ann. Plant Prot. Sci., 19: 106-112.
  14. Karavina, C., Chihiya J. and Tigere, T.A. (2007). Detection and characterization of Xanthomonas phaseoli (E. F. SM) in common bean (Phaseolus vulgaris) seeds collected in Zimbabwe. J. Sustainable Dev. Afr., 10: 105-119.
  15. Khare, D. and Bhale, M.S. (2014). Seed Technology. Scientific Publishers, India, pp. 944.
  16. Kotlarz, A., Sujak, A., Strobel W. and Grzesiak, W. (2011). Chemical composition and nutritive value of protein of the pea seeds-effect of harvesting year and variety. Veg. Crops Res. Bull., 75: 57-69.
  17. Kovacs, N., (1956). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature, 178: 703-703. 
  18. Lawyer, A.S. and Chun, W. (2001). Foliar Disease Caused by Bacteria. In: Compendium of Pea Diseases and Pests, [Kraft, J.M. and F.L. Pfleger (Eds.)]. 2nd Edn., APS Press, St. Paul, pp. 22-24.
  19. Lelliot, R.A. and Stead, D.E. (1987). Methods for the Diagnosis of Bacterial Diseases of Plants. In: Methods in Plant Pathology, Preece, T.F. (Ed.). Blackwell Science, Oxford, pp. 2-216.
  20. Martin-Sanz, A., Palomo, J.L., Vega, M.P.D.L. and Caminero, C. (2011). Identification of pathovars and races of Pseudomonas syringae, the main causal agent of bacterial disease in pea in North-Central Spain, and the search for disease resistance. Eur. J. Plant Pathol., 129: 57-69.
  21. Martin-Sanz, A., Vega, M.P. de la, Murillo, J. and Caminero, C. (2013). Strains of Pseudomonas syringae pv. syringae from pea are phylogenetically and pathogenically diverse. Phytopath., 103: 673-681.
  22. Mohan, S.K. and Schaad, N.W. (1987). An improved agar plating assay for detecting Pseudomonas syringae pv. syringae and P. s. pv. phaseolicola in contaminated bean seed. Phytopath., 77: 1390-1395.
  23. Mortensen, C.N. (1994a). Seed Health Testing for Bacterial Pathogens. Danish Government Institute of Seed Pathology for Developing Countries, Copenhagen, Denmark, pp. 68.
  24. Mortensen, C.N. (1994b). Seed Bacteriology Laboratory Guide. Danish Government Institute of Seed Pathology for Developing Countries, Copenhagen, Denmark, pp. 102.
  25. Rathod, L.R. and Pawar, P.V. (2012). Antimicrobial activity of medicinal plant to control seed borne pathogen of soybean. Curr. Bot., 3: 10-12.
  26. Sacchi, C.T., Whitney, A.M., Mayer, L.W., Morey, R. and Steigerwalt, A. (2002). Sequencing of 16S rRNA gene: A rapid tool for identification of Bacillus anthracis. Emerg. Infect. Dis., 8: 1117-1123.
  27. Sacket, W.G. (1916). A bacterial stem blight of field garden peas. Bull. of the Colorado Agri. Experimental Station. 217: 3-43.
  28. Santos, J., Oliveira, M.B.P.P., Ibanez E. and Herrero, M. (2014). Phenolic profile evolution of different ready-to-eat baby-    leaf vegetables during storage. J. Chromatogr. A., 1327: 118-131.
  29. Taylor, J.D. (1972) Races of Pseudomonas pisi and sources of resistance in field and garden peas. N. Z. J. Agric. Res., 15: 441-447.
  30. Thornley, M.J. (1960). The differentiation of Pseudomonas from other gram negative bacteria on the basis of arginine metabolism. J. Applied Bacteriol.,23:37-52.
     

Global Footprints