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Current IssueEditorial BoardOnline First ArticlesArchive

volume 37 issue 4 (august 2014) : 337-344,   Doi: 10.5958/0976-0571.2014.00641.9

INDUCED MUTAGENESIS IN RECOMBINANTS AND THEIR PARENTS IN SOYBEAN [GLYCINE MAX (L.) MERRIL L]

A
A. Anna Durai*
K
K. Mohanraj
B
B. Subbalakshmi
1Center for Plant Breeding and Genetics Tamil Nadu Agricultural University, Coimbatore- 641 003, India
Cite article:- Durai* Anna A., Mohanraj K., Subbalakshmi B. (2025). INDUCED MUTAGENESIS IN RECOMBINANTS AND THEIR PARENTS IN SOYBEAN [GLYCINE MAX (L.) MERRIL L]. Legume Research. 37(4): 337-344. doi: 10.5958/0976-0571.2014.00641.9.

ABSTRACT

Six soybean accessions viz., TNAU S 7, TNAU S 55, DS 9501, TS 82, Himso 1563 and CO 2 and their 15 F1 and five F2 populations were taken with the objective of studying their response to gamma radiation. Among the parents, TS 82 and Himso 1563 were highly sensitive while CO 2 was less sensitive to the mutagen. Analysis of variance showed significant differences among treatment combinations for all the traits except number of seeds per pod.  High frequency of chlorophyll mutants viz., 4.0 per cent at 3.0 grey for TNAU S 7 and 4.5 per cent for Himso 1563 at 2.5 grey occurred. Morphologically variable viable mutants  during early phase of the crop and aberrants with  modified stem structure, plant structure, dwarf, gigas, stunted, leaf morphology, small leaves, flower colour, pod colour, sterile, partially sterile, four seeded, small seeded, bold seeded, seed coat colour and high yielding mutants during the later stages were observed. High heritability coupled with high genetic advance was noted for plant height (TNAU S 7 / TS 82 at control and at 2.5 grey, and DS 9501 / TS 82 at control), number of clusters per plant (TNAU S 7 / TS 82 at 2.5 grey), total number of pods per plant (DS 9501 / TS 82 at 2.5 grey, TNAU S 7 / TS 82 at control and at 2.5 grey) and total dry matter accumulation (DS 9501 / TS 82 at 3.0 grey). Directional selection in these populations may improve the yield attributes which will in turn increase the grain yield in soybean

REFERENCES

  1. Ahloowalia,B.S., Malusyzynski, M. and Nichterlein, N. (2004). Global impact of mutation derived varieties. Euphytica, 135 (2): 187-204.
  2. Brian, C and Deoborah, C. (1998). Some evolutionary consequences of deleterious mutations. Genetica, 102-103: 3-19.
  3. Burton, G.W. (1952). Quantitative inheritance in grass. Proc.6th Internat. Grassland Cong., 1: 277-283.
  4. Cholakov, D and Petkova, V. (2002). Morphological and physiological characteristics of seedlings caused by laser and gamma irradiation of cucumber seeds Acta Hort. (ISHS), 579: 285-288.
  5. Cimen, A., Sema, A., Leyla A., and Yasemin, C. (2004). Induced plastid mutations in soybean plant (Glycine max (L) Merrill) with gamma radiation and determination with RAPD. Mutation Research/Fundamental and Molecular mechanism of mutagenesis, 556(1): 34-44.
  6. Geetha, K. and Vaidyanathan, P. (1998). Studies on induction of mutation in soybean through physical and chemical mutagens. Agrl. Sci. Digest, 18(1): 27-30.
  7. Hailu, T., Assefa, K., Hundera, F., Kefyalew, T and Teferra, T. (2003). Heritability and Genetic advance in recombinant inbred lines of tef (Eragrostic tef). Euphytica, 131: 91-96.
  8. Johnson, H.W., Robinson, H.F. and Comstock. R.E (1955). Estimates of genetic and environmental variability in Soybean. Agron J., 47: 314-318.
  9. Kundi, R.S., Gill, M.S. and Phul T.P. (1997). Radiation induced variability in quantitative traits in soybean. (Glycine max (L) Merrill). Crop Improve. 24 (12):231-234.
  10. Lush,J.L. (1949). Heritability of quantitative characters in farm animals. Hereditas, 35: 356-375.
  11. Mingue, G. (1986). Multileaflet mutants in soybean. Mutation Breeding Newsletter. Issue No.27.
  12. Sharma, J.R. (1998). Statistics and Biometrical Techniques in plant breeding. New Age Int.(P)Ltd. New Delhi. pp 311.
  13. Sharma, S.K. and Sharma, B. (1984). Pattern of induced macro and micromutations with gamma rays and NMU in Lentil. Environmental and experimental Botany, 24 (4): 343-351.
  14. Sophie, M.P. and Dan, I.A. (2004). Adaptation to the deleterious effects of antimicrobial drug resistance mutations by compensatory evolution. Research in microbiology, 155(5): 360-369.
  15. Xue, B.L., Meng, X.Y., Guo, Z. and Liu. B. (2000). Mutagenic effect of 60 CO gamma irradiation in soybean plants. Soybean Science, 19 (2): 105-110.
  16. Yijian, W., Y. Hongwei and Y. Shufan. (1986). A new soybean cultivar Liaodou No.3 Mutation Breeding Newsletter. Issue No. 27.
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    volume 37 issue 4 (august 2014) : 337-344,   Doi: 10.5958/0976-0571.2014.00641.9

    INDUCED MUTAGENESIS IN RECOMBINANTS AND THEIR PARENTS IN SOYBEAN [GLYCINE MAX (L.) MERRIL L]

    A
    A. Anna Durai*
    K
    K. Mohanraj
    B
    B. Subbalakshmi
    1Center for Plant Breeding and Genetics Tamil Nadu Agricultural University, Coimbatore- 641 003, India
    Cite article:- Durai* Anna A., Mohanraj K., Subbalakshmi B. (2025). INDUCED MUTAGENESIS IN RECOMBINANTS AND THEIR PARENTS IN SOYBEAN [GLYCINE MAX (L.) MERRIL L]. Legume Research. 37(4): 337-344. doi: 10.5958/0976-0571.2014.00641.9.

    ABSTRACT

    Six soybean accessions viz., TNAU S 7, TNAU S 55, DS 9501, TS 82, Himso 1563 and CO 2 and their 15 F1 and five F2 populations were taken with the objective of studying their response to gamma radiation. Among the parents, TS 82 and Himso 1563 were highly sensitive while CO 2 was less sensitive to the mutagen. Analysis of variance showed significant differences among treatment combinations for all the traits except number of seeds per pod.  High frequency of chlorophyll mutants viz., 4.0 per cent at 3.0 grey for TNAU S 7 and 4.5 per cent for Himso 1563 at 2.5 grey occurred. Morphologically variable viable mutants  during early phase of the crop and aberrants with  modified stem structure, plant structure, dwarf, gigas, stunted, leaf morphology, small leaves, flower colour, pod colour, sterile, partially sterile, four seeded, small seeded, bold seeded, seed coat colour and high yielding mutants during the later stages were observed. High heritability coupled with high genetic advance was noted for plant height (TNAU S 7 / TS 82 at control and at 2.5 grey, and DS 9501 / TS 82 at control), number of clusters per plant (TNAU S 7 / TS 82 at 2.5 grey), total number of pods per plant (DS 9501 / TS 82 at 2.5 grey, TNAU S 7 / TS 82 at control and at 2.5 grey) and total dry matter accumulation (DS 9501 / TS 82 at 3.0 grey). Directional selection in these populations may improve the yield attributes which will in turn increase the grain yield in soybean

    REFERENCES

    1. Ahloowalia,B.S., Malusyzynski, M. and Nichterlein, N. (2004). Global impact of mutation derived varieties. Euphytica, 135 (2): 187-204.
    2. Brian, C and Deoborah, C. (1998). Some evolutionary consequences of deleterious mutations. Genetica, 102-103: 3-19.
    3. Burton, G.W. (1952). Quantitative inheritance in grass. Proc.6th Internat. Grassland Cong., 1: 277-283.
    4. Cholakov, D and Petkova, V. (2002). Morphological and physiological characteristics of seedlings caused by laser and gamma irradiation of cucumber seeds Acta Hort. (ISHS), 579: 285-288.
    5. Cimen, A., Sema, A., Leyla A., and Yasemin, C. (2004). Induced plastid mutations in soybean plant (Glycine max (L) Merrill) with gamma radiation and determination with RAPD. Mutation Research/Fundamental and Molecular mechanism of mutagenesis, 556(1): 34-44.
    6. Geetha, K. and Vaidyanathan, P. (1998). Studies on induction of mutation in soybean through physical and chemical mutagens. Agrl. Sci. Digest, 18(1): 27-30.
    7. Hailu, T., Assefa, K., Hundera, F., Kefyalew, T and Teferra, T. (2003). Heritability and Genetic advance in recombinant inbred lines of tef (Eragrostic tef). Euphytica, 131: 91-96.
    8. Johnson, H.W., Robinson, H.F. and Comstock. R.E (1955). Estimates of genetic and environmental variability in Soybean. Agron J., 47: 314-318.
    9. Kundi, R.S., Gill, M.S. and Phul T.P. (1997). Radiation induced variability in quantitative traits in soybean. (Glycine max (L) Merrill). Crop Improve. 24 (12):231-234.
    10. Lush,J.L. (1949). Heritability of quantitative characters in farm animals. Hereditas, 35: 356-375.
    11. Mingue, G. (1986). Multileaflet mutants in soybean. Mutation Breeding Newsletter. Issue No.27.
    12. Sharma, J.R. (1998). Statistics and Biometrical Techniques in plant breeding. New Age Int.(P)Ltd. New Delhi. pp 311.
    13. Sharma, S.K. and Sharma, B. (1984). Pattern of induced macro and micromutations with gamma rays and NMU in Lentil. Environmental and experimental Botany, 24 (4): 343-351.
    14. Sophie, M.P. and Dan, I.A. (2004). Adaptation to the deleterious effects of antimicrobial drug resistance mutations by compensatory evolution. Research in microbiology, 155(5): 360-369.
    15. Xue, B.L., Meng, X.Y., Guo, Z. and Liu. B. (2000). Mutagenic effect of 60 CO gamma irradiation in soybean plants. Soybean Science, 19 (2): 105-110.
    16. Yijian, W., Y. Hongwei and Y. Shufan. (1986). A new soybean cultivar Liaodou No.3 Mutation Breeding Newsletter. Issue No. 27.
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