Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 37 issue 5 (october 2014) : 479-482,   Doi: 10.5958/0976-0571.2014.00663.8

SPECTRUM OF VARIABILITY IN SEED SIZE AND CONTENT OF PROTEIN AND ODAP IN GRASS PEA (LATHYRUS SATIVUS L.) GERMPLASM

M
M.M.A. Mondal1
A
A.B. Puteh*
1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia
Cite article:- Mondal1 M.M.A., Puteh* A.B. (2025). SPECTRUM OF VARIABILITY IN SEED SIZE AND CONTENT OF PROTEIN AND ODAP IN GRASS PEA (LATHYRUS SATIVUS L.) GERMPLASM. Legume Research. 37(5): 479-482. doi: 10.5958/0976-0571.2014.00663.8.

ABSTRACT

Seeds of grass pea contain an unusual amino acid, ß-N-oxalyl-L-2,3-diamino propionic acid (ODAP) also referred as B-(N)-Oxalyl-amino-L-alanine (BOAA) which is responsible for an incurable paralysis of the lower limbs of men, called lathyrism. This study included thirty bold and small seeded genotypes of grass pea to ascertain the variability and the relationship existing among seed size, content of protein and ODAP in seeds. Weight of 1000-seed ranged from 40.50 to 79.23 g. The protein and ODAP content ranged from 25.05 to 33.95% and 0.32 to 2.02%, respectively. A significant positive correlation was observed between seed size and protein content (r = 0.48**) but negative association was obtained between seed size and ODAP content (r = - 0.22*). There was also negative association between the content of protein and ODAP (r = - 0.16). Therefore, it may be feasible to develop low neurotoxin varieties by selecting bold seed size following hybridization of bold seeded and low neurotoxin types with those having small seeds and high neurotoxin.

REFERENCES

  1. Abd El Moneim, A., Van Dorrestein, B., Baum, M., Ryan, J. and Bejiga G. (2001). Role of ICARDA in improving the nutritional quality and yield potential of grasspea (Lathyrus sativus L.) for subsistence farmers in dry areas. Lathyrus Lathyrism Newsl., 2: 55-58.
  2. Basaran, U., Asci, O.O., Mut, H., Acar, Z. and Ayan, I. (2011). Some quality traits and neurotoxin b-N-oxalyl-L-a,b- diaminopropionic acid (â-ODAP) contents of Lathyrus sp. cultivated in Turkey. African J. Biotech., 10: 4072-4080.
  3. Campbell, C.G. (1997). The Grasspea. International Plant Genetic Resources Institute, Via delle Sette Chiese 142, 00145 Rome, Italy. pp. 34-37.
  4. Enneking, D. (2011). The nutritive value of grasspea (Lathyrus sativus) and allied species, their toxicity to animals and the role of malnutrition in neurolathyrism. Food Chem. Toxicol., 49: 694-709.
  5. Girma, A., Tefera, B. and Dadi, L. (2011). Grass pea and neurolathyrism: farmers’ perception on its consumption and protective measure in North Shewa, Ethiopia. Food Chem. Toxicol., 49: 668-672.
  6. Kaul, A.K., Islam, M.Q. and Hamid, A. (1986). Screening of Lathyrus germplasm of Bangladesh for BOAA content and some agronomic characters. In: Kaul AK & Combs D. (ed). Lathyrus and Lathyrism, Third World Medical Research Foundation, New York, pp. 161-168.
  7. Palmer, V.S., Kaul, A.K. and Spencer P.S. (1989). International Network for the Improvement of Lathyrus sativus and the Eradication of Lathyrism (INILSEL): A TWMRF initiative. In: The Grass Pea: Threat and Promise. Proc. of the International Network for the Improvement of Lathyrus sativus and the Eradication of Lathyrism (P. Spencer, ed.), Third World Medical Research Foundation, New York, pp. 219-223.
  8. Polignano, G.B., Uggenti, P., Olita, G., Bisignano, V., Alba, V. and Perrino P. (2005). Characterization of grass pea (Lathyrus sativus L.) entries by means of agronomically useful traits. Lathyrus lathyrism Newsl., 4: 10–14.
  9. Quader, M. (1985). Genetic analysis of neurotoxin content and some aspects of reproductive biology in Lathyrus sativus. PhD Thesis. Indian Agricultural Research Institute, New Delhi, India.
  10. Quader, M., Ramanujam, S. and Baral, G.K. (1986). Genetics of flower colour, BOAA content and their relationship in Lathyrus sativus L. In: Kaul AK & Combs D. (ed). Lathyrus and Lathyrism, Third World Medical Research Foundation, New York, pp. 161-168.
  11. Rao, S.L.N. (1978). A sensitive and specific colorimetric method for determination of á,â-diaminopropionic acid and Lathyrus sativus neurotoxin. Anal. Biochem., 86: 386–395.
  12. Sammour, R.H., Mustafa, A.E., Badr, S. and Tahr W. (2007). Genetic variability of some quality traits in Lathyrus spp. germplasm. Acta Agric. Slovenica, 90: 33-43.
  13. Sharma, R.N., Chitale, M.W., Ganvir, G.B., Geda, A.K. and Pandey R.L. (2001). Observations on the development of selection criterion for high yield and low neurotoxin in grass pea based on genetic resources. Lathyrus Lathyrism Newsl., 1: 15-16.
  14. Talukdar, D. and Biswas, A.K. (2005). Induced seed coat colour mutations and their inheritance in grass pea. Indian J. Genet., 65: 135-136.
  15. Talukdar, D., Biswas, S.C. and Biswas A.K. (2002). An induced flower colour mutant in grass pea. Indian J. Genet., 62: 162-167.
  16. Tamburino, R., Guida, V., Pacifico, S., Rocco, M., Zarelli, A., Parente, A. and Maro, A.D. (2012). Nutritional values and radical scavenging capacities of grass pea (Lathyrus sativus L.) seeds in Valle Agricola district, Italy. Aust. J. Crop Sci., 6: 149-156.
  17. Tiwari, K. R. and Campbell, C.G. (1986). Inheritance of neurotoxin (ODAP) content, flower and seed coat colour in grass pea. Euphytica, 91: 195-203.
  18. Yan, Z.Y., Spencer, P.S., Li, Z.X., Liang, Y.M., Wang, Y.F., Wang, C.Y. and Li, F.M. (2006). Lathyrus sativus (grass pea) and its neurotoxin ODAP. Phytochem., 67: 107-121.
Published In
Legume Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 37 issue 5 (october 2014) : 479-482,   Doi: 10.5958/0976-0571.2014.00663.8

    SPECTRUM OF VARIABILITY IN SEED SIZE AND CONTENT OF PROTEIN AND ODAP IN GRASS PEA (LATHYRUS SATIVUS L.) GERMPLASM

    M
    M.M.A. Mondal1
    A
    A.B. Puteh*
    1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia
    Cite article:- Mondal1 M.M.A., Puteh* A.B. (2025). SPECTRUM OF VARIABILITY IN SEED SIZE AND CONTENT OF PROTEIN AND ODAP IN GRASS PEA (LATHYRUS SATIVUS L.) GERMPLASM. Legume Research. 37(5): 479-482. doi: 10.5958/0976-0571.2014.00663.8.

    ABSTRACT

    Seeds of grass pea contain an unusual amino acid, ß-N-oxalyl-L-2,3-diamino propionic acid (ODAP) also referred as B-(N)-Oxalyl-amino-L-alanine (BOAA) which is responsible for an incurable paralysis of the lower limbs of men, called lathyrism. This study included thirty bold and small seeded genotypes of grass pea to ascertain the variability and the relationship existing among seed size, content of protein and ODAP in seeds. Weight of 1000-seed ranged from 40.50 to 79.23 g. The protein and ODAP content ranged from 25.05 to 33.95% and 0.32 to 2.02%, respectively. A significant positive correlation was observed between seed size and protein content (r = 0.48**) but negative association was obtained between seed size and ODAP content (r = - 0.22*). There was also negative association between the content of protein and ODAP (r = - 0.16). Therefore, it may be feasible to develop low neurotoxin varieties by selecting bold seed size following hybridization of bold seeded and low neurotoxin types with those having small seeds and high neurotoxin.

    REFERENCES

    1. Abd El Moneim, A., Van Dorrestein, B., Baum, M., Ryan, J. and Bejiga G. (2001). Role of ICARDA in improving the nutritional quality and yield potential of grasspea (Lathyrus sativus L.) for subsistence farmers in dry areas. Lathyrus Lathyrism Newsl., 2: 55-58.
    2. Basaran, U., Asci, O.O., Mut, H., Acar, Z. and Ayan, I. (2011). Some quality traits and neurotoxin b-N-oxalyl-L-a,b- diaminopropionic acid (â-ODAP) contents of Lathyrus sp. cultivated in Turkey. African J. Biotech., 10: 4072-4080.
    3. Campbell, C.G. (1997). The Grasspea. International Plant Genetic Resources Institute, Via delle Sette Chiese 142, 00145 Rome, Italy. pp. 34-37.
    4. Enneking, D. (2011). The nutritive value of grasspea (Lathyrus sativus) and allied species, their toxicity to animals and the role of malnutrition in neurolathyrism. Food Chem. Toxicol., 49: 694-709.
    5. Girma, A., Tefera, B. and Dadi, L. (2011). Grass pea and neurolathyrism: farmers’ perception on its consumption and protective measure in North Shewa, Ethiopia. Food Chem. Toxicol., 49: 668-672.
    6. Kaul, A.K., Islam, M.Q. and Hamid, A. (1986). Screening of Lathyrus germplasm of Bangladesh for BOAA content and some agronomic characters. In: Kaul AK & Combs D. (ed). Lathyrus and Lathyrism, Third World Medical Research Foundation, New York, pp. 161-168.
    7. Palmer, V.S., Kaul, A.K. and Spencer P.S. (1989). International Network for the Improvement of Lathyrus sativus and the Eradication of Lathyrism (INILSEL): A TWMRF initiative. In: The Grass Pea: Threat and Promise. Proc. of the International Network for the Improvement of Lathyrus sativus and the Eradication of Lathyrism (P. Spencer, ed.), Third World Medical Research Foundation, New York, pp. 219-223.
    8. Polignano, G.B., Uggenti, P., Olita, G., Bisignano, V., Alba, V. and Perrino P. (2005). Characterization of grass pea (Lathyrus sativus L.) entries by means of agronomically useful traits. Lathyrus lathyrism Newsl., 4: 10–14.
    9. Quader, M. (1985). Genetic analysis of neurotoxin content and some aspects of reproductive biology in Lathyrus sativus. PhD Thesis. Indian Agricultural Research Institute, New Delhi, India.
    10. Quader, M., Ramanujam, S. and Baral, G.K. (1986). Genetics of flower colour, BOAA content and their relationship in Lathyrus sativus L. In: Kaul AK & Combs D. (ed). Lathyrus and Lathyrism, Third World Medical Research Foundation, New York, pp. 161-168.
    11. Rao, S.L.N. (1978). A sensitive and specific colorimetric method for determination of á,â-diaminopropionic acid and Lathyrus sativus neurotoxin. Anal. Biochem., 86: 386–395.
    12. Sammour, R.H., Mustafa, A.E., Badr, S. and Tahr W. (2007). Genetic variability of some quality traits in Lathyrus spp. germplasm. Acta Agric. Slovenica, 90: 33-43.
    13. Sharma, R.N., Chitale, M.W., Ganvir, G.B., Geda, A.K. and Pandey R.L. (2001). Observations on the development of selection criterion for high yield and low neurotoxin in grass pea based on genetic resources. Lathyrus Lathyrism Newsl., 1: 15-16.
    14. Talukdar, D. and Biswas, A.K. (2005). Induced seed coat colour mutations and their inheritance in grass pea. Indian J. Genet., 65: 135-136.
    15. Talukdar, D., Biswas, S.C. and Biswas A.K. (2002). An induced flower colour mutant in grass pea. Indian J. Genet., 62: 162-167.
    16. Tamburino, R., Guida, V., Pacifico, S., Rocco, M., Zarelli, A., Parente, A. and Maro, A.D. (2012). Nutritional values and radical scavenging capacities of grass pea (Lathyrus sativus L.) seeds in Valle Agricola district, Italy. Aust. J. Crop Sci., 6: 149-156.
    17. Tiwari, K. R. and Campbell, C.G. (1986). Inheritance of neurotoxin (ODAP) content, flower and seed coat colour in grass pea. Euphytica, 91: 195-203.
    18. Yan, Z.Y., Spencer, P.S., Li, Z.X., Liang, Y.M., Wang, Y.F., Wang, C.Y. and Li, F.M. (2006). Lathyrus sativus (grass pea) and its neurotoxin ODAP. Phytochem., 67: 107-121.
    In this Article
      Contact us
      Follow us
      Published In
      Legume Research

      Editorial Board

      View all (0)