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Current IssueEditorial BoardOnline First ArticlesArchive

volume 36 issue 4 (august 2013) : 289-298

ASSESSMENT OF GENETIC DIVERSITY AMONG INDIAN FENUGREEK (TRIGOI ELLA FOENUM-GRAECUM L.) VARIETIES USING MORPHOLOGICAL AND RAPD MARKERS

S
Sharda Choudhary*
R
R.S. Meena
R
Ravindra Singh
M
M.K. Vishal
V
Vibha Choudhary
A
Alka Panwar
1National Research Centre on Seed Spices, Tabiji, Ajmer - 305 206, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Choudhary* Sharda, Meena R.S., Singh Ravindra, Vishal M.K., Choudhary Vibha, Panwar Alka (2025). ASSESSMENT OF GENETIC DIVERSITY AMONG INDIAN FENUGREEK (TRIGOI ELLA FOENUM-GRAECUM L.) VARIETIES USING MORPHOLOGICAL AND RAPD MARKERS. Legume Research. 36(4): 289-298. doi: .

ABSTRACT

The study was conducted to reveal genetic variability among the released varieties of fenugreek from different regions of India using morphological and molecular markers. Seventeen varieties were evaluated for important characteristics like plant height, test weight, total weight, yield per plant, pod length, pod per plant, pod per axis, petiole length and 50% flowering. In this study, Random Amplified Polymorphic DNA (RAPD) markers were used to assess genetic diversity wherein fifteen polymorphic primers showed a 57.66% polymorphism. All the varieties were classified into two major clusters viz., cluster-I and cluster-II, cluster-I is further divided into five sub clusters containing twelve varieties namely Hisar Suvarna, Hisar Sonali, Rajendra Kranti, Hisar Mukta, Hisar Madhavi, AM-2, GM-2, AM-1, Azad Methi, CO-2, RMT-143 and RMT-351 showing genetic similarity irrespective of their geographical distribution. Cluster-II contains five varieties viz., Lam Selection, RMT-1, RMT-305, RMT-303 and Pant Ragini. In morphological analysis few varieties did not follow the pattern of RAPD analysis indicating their phenotypic characters to be influenced by the environmental factors. Morphological dendrogram also showed two major clusters and Pant Ragini was found to branch out distinctly confirming its uniqueness from all other varieties.

REFERENCES

  1. Dangi, RS., Lagu, M.D., Choudhary, L.B., Ranjekar, P.K., Gupta, VS. (2004) Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caerulea using ISSR and RAPD markers. BMC Plant Biol. 4, 1–11.
  2. Eujayl, I., Baum, M., Erskine, W., Pehu, E. (1998). Agenetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines. Theor. Appl. Genet. 97, 83-89
  3. Fracaro, F., Jucimar, Z., Sergio, E. (2005). RAPD based genetic relationships between populations of three chemotypes of Cunila galioides Benth. Bochem. Syst. Ecol. 33, 409-417.
  4. Kakani, R.K., Singh, S.K., Pancholy, A., Meena, R.S., Pathak, R., Raturi, A. (2011). Assessment of Genetic Diversity in Trigonella foenum-graecum Based on Nuclear Ribosomal DNA, Internal Transcribed Spacer and RAPD Analysis. Plant Mol Biol Rep. 29, 315–323.
  5. Khan, S.A., Awan, F.S., Ahmad, A., Fu, Y.B., Iqbal, A. (2005). Genetic diversity of Pakistan wheat germplasm as revealed by RAPD markers. Genetic Resources and Crop Evolution. 52, 239-244.
  6. Meerow, A.W. (2005). Molecular genetic characterization of new floricultural germplasm. Acta Horticulturae. 683, 43-62.
  7. Mir, A. N., Nabulsi, I. (2003). Genetic diversity of almond (Prunus dulcis) using RAPD technique. Hort. Sci. 98, 461- 471.
  8. Rohlf, F.J. (1998). NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, Version 2.00. Exeter Software. Setauket, New York.
  9. Shiran, B., Amirbakhtiar, N., Kiani, S., Mohammadi, S., Sayed-Tabatabaei, B.E., Moradi, H. (2007). Molecular characterization and genetic relationship among almond cultivars assessed by RAPD and SSR markers. Sci. Hort. 111, 280-290.
  10. Staub, J.E., Box, J., Meglic, V., Horejsi, T., Mc Creight, J.D. (1997). Utility of random amplified polymorphic DNA’s for determining in transpacific relationship in Cucumis. Genet. Res. Crop Evol. USDA/ARS, Univ. Wisconsin, Hort. Deptt. Madison, USA.
  11. Welsh, J., Mclelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acid Res. 18, 7213-7218.
  12. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. , Tingey, S.V. (1990). DNA Polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535.
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    volume 36 issue 4 (august 2013) : 289-298

    ASSESSMENT OF GENETIC DIVERSITY AMONG INDIAN FENUGREEK (TRIGOI ELLA FOENUM-GRAECUM L.) VARIETIES USING MORPHOLOGICAL AND RAPD MARKERS

    S
    Sharda Choudhary*
    R
    R.S. Meena
    R
    Ravindra Singh
    M
    M.K. Vishal
    V
    Vibha Choudhary
    A
    Alka Panwar
    1National Research Centre on Seed Spices, Tabiji, Ajmer - 305 206, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Choudhary* Sharda, Meena R.S., Singh Ravindra, Vishal M.K., Choudhary Vibha, Panwar Alka (2025). ASSESSMENT OF GENETIC DIVERSITY AMONG INDIAN FENUGREEK (TRIGOI ELLA FOENUM-GRAECUM L.) VARIETIES USING MORPHOLOGICAL AND RAPD MARKERS. Legume Research. 36(4): 289-298. doi: .

    ABSTRACT

    The study was conducted to reveal genetic variability among the released varieties of fenugreek from different regions of India using morphological and molecular markers. Seventeen varieties were evaluated for important characteristics like plant height, test weight, total weight, yield per plant, pod length, pod per plant, pod per axis, petiole length and 50% flowering. In this study, Random Amplified Polymorphic DNA (RAPD) markers were used to assess genetic diversity wherein fifteen polymorphic primers showed a 57.66% polymorphism. All the varieties were classified into two major clusters viz., cluster-I and cluster-II, cluster-I is further divided into five sub clusters containing twelve varieties namely Hisar Suvarna, Hisar Sonali, Rajendra Kranti, Hisar Mukta, Hisar Madhavi, AM-2, GM-2, AM-1, Azad Methi, CO-2, RMT-143 and RMT-351 showing genetic similarity irrespective of their geographical distribution. Cluster-II contains five varieties viz., Lam Selection, RMT-1, RMT-305, RMT-303 and Pant Ragini. In morphological analysis few varieties did not follow the pattern of RAPD analysis indicating their phenotypic characters to be influenced by the environmental factors. Morphological dendrogram also showed two major clusters and Pant Ragini was found to branch out distinctly confirming its uniqueness from all other varieties.

    REFERENCES

    1. Dangi, RS., Lagu, M.D., Choudhary, L.B., Ranjekar, P.K., Gupta, VS. (2004) Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caerulea using ISSR and RAPD markers. BMC Plant Biol. 4, 1–11.
    2. Eujayl, I., Baum, M., Erskine, W., Pehu, E. (1998). Agenetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines. Theor. Appl. Genet. 97, 83-89
    3. Fracaro, F., Jucimar, Z., Sergio, E. (2005). RAPD based genetic relationships between populations of three chemotypes of Cunila galioides Benth. Bochem. Syst. Ecol. 33, 409-417.
    4. Kakani, R.K., Singh, S.K., Pancholy, A., Meena, R.S., Pathak, R., Raturi, A. (2011). Assessment of Genetic Diversity in Trigonella foenum-graecum Based on Nuclear Ribosomal DNA, Internal Transcribed Spacer and RAPD Analysis. Plant Mol Biol Rep. 29, 315–323.
    5. Khan, S.A., Awan, F.S., Ahmad, A., Fu, Y.B., Iqbal, A. (2005). Genetic diversity of Pakistan wheat germplasm as revealed by RAPD markers. Genetic Resources and Crop Evolution. 52, 239-244.
    6. Meerow, A.W. (2005). Molecular genetic characterization of new floricultural germplasm. Acta Horticulturae. 683, 43-62.
    7. Mir, A. N., Nabulsi, I. (2003). Genetic diversity of almond (Prunus dulcis) using RAPD technique. Hort. Sci. 98, 461- 471.
    8. Rohlf, F.J. (1998). NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, Version 2.00. Exeter Software. Setauket, New York.
    9. Shiran, B., Amirbakhtiar, N., Kiani, S., Mohammadi, S., Sayed-Tabatabaei, B.E., Moradi, H. (2007). Molecular characterization and genetic relationship among almond cultivars assessed by RAPD and SSR markers. Sci. Hort. 111, 280-290.
    10. Staub, J.E., Box, J., Meglic, V., Horejsi, T., Mc Creight, J.D. (1997). Utility of random amplified polymorphic DNA’s for determining in transpacific relationship in Cucumis. Genet. Res. Crop Evol. USDA/ARS, Univ. Wisconsin, Hort. Deptt. Madison, USA.
    11. Welsh, J., Mclelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acid Res. 18, 7213-7218.
    12. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. , Tingey, S.V. (1990). DNA Polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535.
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