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volume 36 issue 4 (august 2013) : 299-305

PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF CLUSTERBEAN [Cyamopsis tetragonoloba (L.) Taub] RHIZOBIA ISOLATED FROM DIFFERENT AREAS OF RAJASTHAN, india

B
Brijesh Kumar Mishra1*
V
Virendra Yadav
M
M K Vishal1
K
Krishna Kant1
1Department of Molecular Biology and Biotechnology, MPUAT, Udaipur- 313 001, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Mishra1* Kumar Brijesh, Yadav Virendra, Vishal1 K M, Kant1 Krishna (2025). PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF CLUSTERBEAN [Cyamopsis tetragonoloba (L.) Taub] RHIZOBIA ISOLATED FROM DIFFERENT AREAS OF RAJASTHAN, india. Legume Research. 36(4): 299-305. doi: .

ABSTRACT

Cluster bean [Cyamopsis tetragonoloba (L.) Taub, 2n=14] is an important self-pollinated legume crop. In Rajasthan it has become very popular crop in last decade. Cluster bean nodulating bacteria are highly specific for their host plant. As these bacteria do not show any serological cross- reaction with known rhizobia species, they are unique. The present investigation was carried out by isolating bacteria nodulating cluster bean from the areas of arid and semi-arid regions of Rajasthan to identify effective and competitive strains tolerant to various abiotic stresses such as temperature, pH and salinity. A total of 15 rhizobium isolates from cluster bean nodules were characterized on the basis of cultural analysis and screened for physiological traits. Most isolates were tolerant to pH between 6.0 to 8.0, NaCl concentration between 0.5 to 3% and temperature between 37 to 420C. These rhizobial isolates were characterized by random amplified polymorphic DNA to estimate their relationship on molecular basis and compared with their tolerance to various abiotic stresses. The rhizobial isolates from hot climatic area are having more tolerance to abiotic stress specially temperature, among these few rhizobial isolates which are genetically similar, also are less tolerance to abiotic stress and grouped in same cluster on the dendogram.

REFERENCES

  1. Dadarwal, K.R. (1980). Host bacterium factors involved in legume symbiosis. Indian J. Microbiol. 20:245-252.
  2. El Sheikh, E.A.E. and Wood, M. (1989). Response of Cluster bean and soybean rhizobia to salt: osmotic and specific ion effects of salts. Soil Biol. Biochem. 21:889-985.
  3. El Sheikh, E.A.E. and Wood, M.(1990). Salt effects on survival and multiplication of Cluster bean and soybean rhizobia. Soil Biol. Biochem. 22:343-347.
  4. Graham, P.H. (1992). Stress tolerance in Rhizobium and Bradyrhizobium and nodulation under adverse soil conditions. Canadian J. Microbiol. 38:475-484.
  5. Graham, P.H., Sandowsky, M.J., Keyser, H.H., Barnet, Y.M., Bradley, R.S., Cooper, J.E., De LEy, J., Jarvis, B.D.W., Roslyeky, E.B., Stijdom, B.W. and Young, J.P.W. (1991). Proposed minimal standards for the description of new genera and species of root and stem nodulating bacteria. International J. Systematic Bacteriol. 41:582-587.
  6. Jordan, D.C. (1984). Family III. Rhizobiaceae. In: Krieg, N.R., Holt, J.G. (Eds). Bergey’s Manual of Systematic Bacteriology. Willams and Wilkins, Baltimore, pp.234-242.
  7. Kucuk, C. and Kivanc, M. (2008). Preliminary characterization of Rhizobium strains isolated from Cluster bean nodules. African J. Biotechnol. 7:772-775.
  8. Lauter, D.J., Munas, D.N. and Clarkin, K.L. (1981). Salt response of Cluster bean as influenced by N supply. Agron J. 73:167-169.
  9. Maatallah, J., Berraho, E.B., Munoz, S., Sanjun, J. and Lluch, C. (2002). Phenotypic and molecular characterization of Cluster bean rhizobia isolated from different areas of Morocco. J. Applied Microbiol. 93:531-540.
  10. Mpepereki, S., Makonese, F., Wallum, A.G.(1997). Physiological characterization of indigenous rhizobia nodulating Vigna unguculata in zimbabwean soil. Symbiosis 22:275-292.
  11. Rajasundari, K., Ilamurugu, K. and Logeshwaran, P. (2009). Genetic diversity in rhizobial isolates determined by RAPDs. African J. Biotechnol. 8:2677-2681.
  12. Rolf, F.J. (1997). NTSYS-PC numerical taxonomy and multivariate analysis system, version 2.02. Exeter software, Setauket, New York.
  13. Saghai-Maroof, M.A., Soliman, K., Jorgensen, R.A. and Allard, R.W. (1984). Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Nat. Academy Sci. 81:8014-8018.
  14. Sambrook, J., Fritisch, E.F. and Mariaties, T. (1989). Molecular cloning. A laboratory manual 2nd edition, CSH Laboratory Press, Cold Spring Harbowr, New York.
  15. Sharma, N., Sudarsan, Y., Sharma, R. and Singh, G. (2008). RAPD analysis of soil microbial diversity in western Rajasthan. Current Sci. 94:1058-1061.
  16. Vincent, J.M. (1970). A manual for practical study of root nodule bacteria. I.B.P. Hand Book No. 15, Blackwell Scientific Publications Ltd. Oxford, pp.1-13.
  17. Williams, K., Kubelik, A., Livak, K., Rafalski, J. and Tingey, V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535.
  18. Yang, J.X., Yuan, T.Y., Zhang, W.T., Zhou, J.C. and Li, Y.G. (2008). Polyphasic characterization of mungbean (Vigna radiata L.) rhizobia from different geographical regions of China. Soil Biol. Biochem. 40:1681-1688
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    volume 36 issue 4 (august 2013) : 299-305

    PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF CLUSTERBEAN [Cyamopsis tetragonoloba (L.) Taub] RHIZOBIA ISOLATED FROM DIFFERENT AREAS OF RAJASTHAN, india

    B
    Brijesh Kumar Mishra1*
    V
    Virendra Yadav
    M
    M K Vishal1
    K
    Krishna Kant1
    1Department of Molecular Biology and Biotechnology, MPUAT, Udaipur- 313 001, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Mishra1* Kumar Brijesh, Yadav Virendra, Vishal1 K M, Kant1 Krishna (2025). PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF CLUSTERBEAN [Cyamopsis tetragonoloba (L.) Taub] RHIZOBIA ISOLATED FROM DIFFERENT AREAS OF RAJASTHAN, india. Legume Research. 36(4): 299-305. doi: .

    ABSTRACT

    Cluster bean [Cyamopsis tetragonoloba (L.) Taub, 2n=14] is an important self-pollinated legume crop. In Rajasthan it has become very popular crop in last decade. Cluster bean nodulating bacteria are highly specific for their host plant. As these bacteria do not show any serological cross- reaction with known rhizobia species, they are unique. The present investigation was carried out by isolating bacteria nodulating cluster bean from the areas of arid and semi-arid regions of Rajasthan to identify effective and competitive strains tolerant to various abiotic stresses such as temperature, pH and salinity. A total of 15 rhizobium isolates from cluster bean nodules were characterized on the basis of cultural analysis and screened for physiological traits. Most isolates were tolerant to pH between 6.0 to 8.0, NaCl concentration between 0.5 to 3% and temperature between 37 to 420C. These rhizobial isolates were characterized by random amplified polymorphic DNA to estimate their relationship on molecular basis and compared with their tolerance to various abiotic stresses. The rhizobial isolates from hot climatic area are having more tolerance to abiotic stress specially temperature, among these few rhizobial isolates which are genetically similar, also are less tolerance to abiotic stress and grouped in same cluster on the dendogram.

    REFERENCES

    1. Dadarwal, K.R. (1980). Host bacterium factors involved in legume symbiosis. Indian J. Microbiol. 20:245-252.
    2. El Sheikh, E.A.E. and Wood, M. (1989). Response of Cluster bean and soybean rhizobia to salt: osmotic and specific ion effects of salts. Soil Biol. Biochem. 21:889-985.
    3. El Sheikh, E.A.E. and Wood, M.(1990). Salt effects on survival and multiplication of Cluster bean and soybean rhizobia. Soil Biol. Biochem. 22:343-347.
    4. Graham, P.H. (1992). Stress tolerance in Rhizobium and Bradyrhizobium and nodulation under adverse soil conditions. Canadian J. Microbiol. 38:475-484.
    5. Graham, P.H., Sandowsky, M.J., Keyser, H.H., Barnet, Y.M., Bradley, R.S., Cooper, J.E., De LEy, J., Jarvis, B.D.W., Roslyeky, E.B., Stijdom, B.W. and Young, J.P.W. (1991). Proposed minimal standards for the description of new genera and species of root and stem nodulating bacteria. International J. Systematic Bacteriol. 41:582-587.
    6. Jordan, D.C. (1984). Family III. Rhizobiaceae. In: Krieg, N.R., Holt, J.G. (Eds). Bergey’s Manual of Systematic Bacteriology. Willams and Wilkins, Baltimore, pp.234-242.
    7. Kucuk, C. and Kivanc, M. (2008). Preliminary characterization of Rhizobium strains isolated from Cluster bean nodules. African J. Biotechnol. 7:772-775.
    8. Lauter, D.J., Munas, D.N. and Clarkin, K.L. (1981). Salt response of Cluster bean as influenced by N supply. Agron J. 73:167-169.
    9. Maatallah, J., Berraho, E.B., Munoz, S., Sanjun, J. and Lluch, C. (2002). Phenotypic and molecular characterization of Cluster bean rhizobia isolated from different areas of Morocco. J. Applied Microbiol. 93:531-540.
    10. Mpepereki, S., Makonese, F., Wallum, A.G.(1997). Physiological characterization of indigenous rhizobia nodulating Vigna unguculata in zimbabwean soil. Symbiosis 22:275-292.
    11. Rajasundari, K., Ilamurugu, K. and Logeshwaran, P. (2009). Genetic diversity in rhizobial isolates determined by RAPDs. African J. Biotechnol. 8:2677-2681.
    12. Rolf, F.J. (1997). NTSYS-PC numerical taxonomy and multivariate analysis system, version 2.02. Exeter software, Setauket, New York.
    13. Saghai-Maroof, M.A., Soliman, K., Jorgensen, R.A. and Allard, R.W. (1984). Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Nat. Academy Sci. 81:8014-8018.
    14. Sambrook, J., Fritisch, E.F. and Mariaties, T. (1989). Molecular cloning. A laboratory manual 2nd edition, CSH Laboratory Press, Cold Spring Harbowr, New York.
    15. Sharma, N., Sudarsan, Y., Sharma, R. and Singh, G. (2008). RAPD analysis of soil microbial diversity in western Rajasthan. Current Sci. 94:1058-1061.
    16. Vincent, J.M. (1970). A manual for practical study of root nodule bacteria. I.B.P. Hand Book No. 15, Blackwell Scientific Publications Ltd. Oxford, pp.1-13.
    17. Williams, K., Kubelik, A., Livak, K., Rafalski, J. and Tingey, V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535.
    18. Yang, J.X., Yuan, T.Y., Zhang, W.T., Zhou, J.C. and Li, Y.G. (2008). Polyphasic characterization of mungbean (Vigna radiata L.) rhizobia from different geographical regions of China. Soil Biol. Biochem. 40:1681-1688
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