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volume 33 issue 3 (september 2010) : 184 - 189

EVALUATION OF COUNTRY BEAN (DOLICHOS LABLAB) LAND RACES OF NORTH EAST INDIA FOR NUTRITIVE VALUES AND CHARACTERIZATION THROUGH SEED PROTEIN PROFILING.

B
Barnali Sarma
A
Aniruddha Sarma*
G
Gautam K. Handique1
A
A.K. Handique
1Department of Biotechnology, Gauhati University, Guwahati – 781 014, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Sarma Barnali, Sarma* Aniruddha, Handique1 K. Gautam, Handique A.K. (2025). EVALUATION OF COUNTRY BEAN (DOLICHOS LABLAB) LAND RACES OF NORTH EAST INDIA FOR NUTRITIVE VALUES AND CHARACTERIZATION THROUGH SEED PROTEIN PROFILING.. Legume Research. 33(3): 184 - 189. doi: .

ABSTRACT

Nine land races of country bean from North east India were evaluated for nutritive
values and seed protein profile was analyzed for molecular characterization. The tender
pods, which are used as vegetable, had crude protein in the range of 16.44 to 21.47%. Total
carbohydrate varied from 14.53 to 19.61%. Lipid occurred in the lowest proportion; in the
range of 0.43 to 0.96%. The range of variation for crude fiber was 13.53 to 21.47% while for
ash content the range was 6.23 to 9.17%. On the other hand, protein and carbohydrate
content of mature seeds were much higher than the corresponding values for tender pods.
Protein content of mature seeds varied from 23.99 to 35.51% while the range for carbohydrate
was 28.18 to 48.41%. Lipid content varied from 0.76 to 1.93%. Crude fiber contents were
lower than the corresponding values for tender pods and varied from 10.52 to 16.77%.
Range of variation for ash content was 5.13 to 9.19%, which is nearly same as that of tender
pods. SDS-PAGE analysis of seed protein revealed the presence of a total 30 bands and
dendrogram analysis indicated the presence of 2 clusters. 6 protein bands, viz. 31.5, 32.2,
33.6, 40.0, 54.0 and 61.3 Kd were found in all the land races and could be considered as
protein molecular marker for the species.

REFERENCES

  1. Belitz, H.D. and Grosch W. (1999) Food Chemistry, 2nd Edition. Springer Verlag, Berlin, p.694-696.
  2. Chandran K.; Rajagopal K. and Radhakrishnan T. (2002) Electrophoretic study on seed storage proteins of ground nut
  3. (Arachis hypogea L.) cultivars of India. Indian J. Pl Genetic Resources. 15 (2) : 108-111.
  4. Clegg, K.M. (1956) The application of anthrone reagent to the estimation of starch in cereals. J. Food Sci.
  5. Agri., 7 : 40-44
  6. Cook, R. J. (1989) The characterization and identification of crop cultivars by electrophoresis. Electrophoresis. 5 : 65-72
  7. Gomathinayagm, P. and Ramaswamy N.M. (1994) Seed protein pattern of Cowpea (Vigna unguiculata L) and its
  8. distant species. J. Plant Biochem Biotechnol, 3 (2); 149-151.
  9. Gopalan, G. Rama Sastry, B.V. and balasubramaniam, S.C., (1989) Nutritive values of Indian Foods. National Institute
  10. of Nutrition, Hyderabad, India. P. 3-4, 26, 53-54.
  11. Gurumoorthi, P and Janardhanan, K. (2008) Physico-chemical properties and cooking quality of velvet bean (Mucuna
  12. pruriens var utilis) Germplasm. Indian J. Agric. Biochem. 21 (1&2): 31- 34.
  13. Ladizinsky, G. and Hymowitz, J. (1979) Seed protein electrophoresis in taxonomy and evolutionary studies. Theor.
  14. Appl. Genetics., 54: 145-151
  15. Laemmli, U.K., (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
  16. Nature, 227: 680-685.
  17. Latha, M.; Mani, S.; Markose, baby Lissy; Abraham, Z; Ashokan Nair; R. Lal; Hanuman and Misra, S. K. (2009)
  18. Collection and characterization of field bean (Lablba purpureus L Sweet var purpureus). Indian J Plant Genet
  19. Resour. 22(2):93-97.
  20. Vol. 33, No. 3, 2010 189
  21. Mao, A.A. Hynniewta T.M., (2000) Floristic diversity of North East India. J Assam Sci Soc. 41 (4):
  22. 255-266.
  23. Nei, M and Li, W. (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc.
  24. Nalt. Acad. Sci. USA. 74: 5269-5273.
  25. Purseglove, J.W. (1984) Tropical crops: Dicotyledons Vol.1 & 2. The ELBS and Longman, Englandf. 273-283 p.
  26. Sadasivam, S. and Manikam A., (1996) Biochemical Methods for Agriculture Sciences. Wiley Eastern Ltd.
  27. New Delhi. P.20.
  28. Sherman, H.C., (1952) Chemistry of Food and Nutrition. The Macmillan Company, New York. P. 721.
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    volume 33 issue 3 (september 2010) : 184 - 189

    EVALUATION OF COUNTRY BEAN (DOLICHOS LABLAB) LAND RACES OF NORTH EAST INDIA FOR NUTRITIVE VALUES AND CHARACTERIZATION THROUGH SEED PROTEIN PROFILING.

    B
    Barnali Sarma
    A
    Aniruddha Sarma*
    G
    Gautam K. Handique1
    A
    A.K. Handique
    1Department of Biotechnology, Gauhati University, Guwahati – 781 014, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Sarma Barnali, Sarma* Aniruddha, Handique1 K. Gautam, Handique A.K. (2025). EVALUATION OF COUNTRY BEAN (DOLICHOS LABLAB) LAND RACES OF NORTH EAST INDIA FOR NUTRITIVE VALUES AND CHARACTERIZATION THROUGH SEED PROTEIN PROFILING.. Legume Research. 33(3): 184 - 189. doi: .

    ABSTRACT

    Nine land races of country bean from North east India were evaluated for nutritive
    values and seed protein profile was analyzed for molecular characterization. The tender
    pods, which are used as vegetable, had crude protein in the range of 16.44 to 21.47%. Total
    carbohydrate varied from 14.53 to 19.61%. Lipid occurred in the lowest proportion; in the
    range of 0.43 to 0.96%. The range of variation for crude fiber was 13.53 to 21.47% while for
    ash content the range was 6.23 to 9.17%. On the other hand, protein and carbohydrate
    content of mature seeds were much higher than the corresponding values for tender pods.
    Protein content of mature seeds varied from 23.99 to 35.51% while the range for carbohydrate
    was 28.18 to 48.41%. Lipid content varied from 0.76 to 1.93%. Crude fiber contents were
    lower than the corresponding values for tender pods and varied from 10.52 to 16.77%.
    Range of variation for ash content was 5.13 to 9.19%, which is nearly same as that of tender
    pods. SDS-PAGE analysis of seed protein revealed the presence of a total 30 bands and
    dendrogram analysis indicated the presence of 2 clusters. 6 protein bands, viz. 31.5, 32.2,
    33.6, 40.0, 54.0 and 61.3 Kd were found in all the land races and could be considered as
    protein molecular marker for the species.

    REFERENCES

    1. Belitz, H.D. and Grosch W. (1999) Food Chemistry, 2nd Edition. Springer Verlag, Berlin, p.694-696.
    2. Chandran K.; Rajagopal K. and Radhakrishnan T. (2002) Electrophoretic study on seed storage proteins of ground nut
    3. (Arachis hypogea L.) cultivars of India. Indian J. Pl Genetic Resources. 15 (2) : 108-111.
    4. Clegg, K.M. (1956) The application of anthrone reagent to the estimation of starch in cereals. J. Food Sci.
    5. Agri., 7 : 40-44
    6. Cook, R. J. (1989) The characterization and identification of crop cultivars by electrophoresis. Electrophoresis. 5 : 65-72
    7. Gomathinayagm, P. and Ramaswamy N.M. (1994) Seed protein pattern of Cowpea (Vigna unguiculata L) and its
    8. distant species. J. Plant Biochem Biotechnol, 3 (2); 149-151.
    9. Gopalan, G. Rama Sastry, B.V. and balasubramaniam, S.C., (1989) Nutritive values of Indian Foods. National Institute
    10. of Nutrition, Hyderabad, India. P. 3-4, 26, 53-54.
    11. Gurumoorthi, P and Janardhanan, K. (2008) Physico-chemical properties and cooking quality of velvet bean (Mucuna
    12. pruriens var utilis) Germplasm. Indian J. Agric. Biochem. 21 (1&2): 31- 34.
    13. Ladizinsky, G. and Hymowitz, J. (1979) Seed protein electrophoresis in taxonomy and evolutionary studies. Theor.
    14. Appl. Genetics., 54: 145-151
    15. Laemmli, U.K., (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    16. Nature, 227: 680-685.
    17. Latha, M.; Mani, S.; Markose, baby Lissy; Abraham, Z; Ashokan Nair; R. Lal; Hanuman and Misra, S. K. (2009)
    18. Collection and characterization of field bean (Lablba purpureus L Sweet var purpureus). Indian J Plant Genet
    19. Resour. 22(2):93-97.
    20. Vol. 33, No. 3, 2010 189
    21. Mao, A.A. Hynniewta T.M., (2000) Floristic diversity of North East India. J Assam Sci Soc. 41 (4):
    22. 255-266.
    23. Nei, M and Li, W. (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc.
    24. Nalt. Acad. Sci. USA. 74: 5269-5273.
    25. Purseglove, J.W. (1984) Tropical crops: Dicotyledons Vol.1 & 2. The ELBS and Longman, Englandf. 273-283 p.
    26. Sadasivam, S. and Manikam A., (1996) Biochemical Methods for Agriculture Sciences. Wiley Eastern Ltd.
    27. New Delhi. P.20.
    28. Sherman, H.C., (1952) Chemistry of Food and Nutrition. The Macmillan Company, New York. P. 721.
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