SCREENING OF BLACKGRAM GENOTYPES UNDER ARTIFICIALLY INOCULATED CONDITIONS AND BASED ON MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE

Article Id: ARCC1269 | Page : 17 - 22
Citation :- SCREENING OF BLACKGRAM GENOTYPES UNDER ARTIFICIALLY INOCULATED CONDITIONS AND BASED ON MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE.Legume Research-An International Journal.2010.(33):17 - 22
L. Prasanthi, B.V. Bhaskara Reddy, K. Rekha Rani, T. Rajeswari, Y. Sivaprasad, and K. Raja Reddy.
Address : Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati-517502, India.

Abstract

Powdery mildew is an important disease in blackgram which causes severe yield loses. Screening
of 15 lines along with LBG 17 resistant check for powdery mildew under artificial inoculation identified
3 lines having 0-2% plant disease incidence and grouped under 0-1 disease score. PCR reactions
using different primers with genomic DNA of these lines resulted in identification of three resistant
sources with specific amplification for resistance to powdery mildew at 900bp with OPT16 primer.
Considering the powdery mildew reaction and resistance linked RAPD marker, it was possible to
identify the new resistance sources in a short time and they could be utilized in breeding programme
or for direct release.

Keywords

Blackgram Powdery mildew Molecular marker Scar marker

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