Incorporation of Microencapsulated Probiotic Lactic Acid Bacteria in Yoghurt

¹Department Food Processing Technology, College of Food and Dairy Technology, Tamil Nadu Veterinary and Animal Sciences University, Koduveli, Chennai-600 052, Tamil Nadu, India.

ABSTRACT

Background: Probiotics are live microorganisms known for the many health benefits they confer on their host. Lactobacillus acidophilus is a lactose fermenting bacterium that is found naturally in the human body and is also found to exhibit probiotic properties. The gastrointestinal tract of humans is however, a harsh environment for microorganisms to survive and render the intended health benefits. Microencapsulation is a technique by which a substance is enveloped by another, forming a capsule that may range up to several hundred microns in size. In this study the probiotic organism L. acidophilus was microencapsulated in a hydrocolloid bead (alginate) and then incorporated in yoghurt.
Methods: Probiotic properties of Lactobacillus acidophilus was evaluated. It was then micro encapuslated in 50 per cent carrot juice and then incorporated in yoghurt. Nutrient composition of this value added yoghurt was studied and its sensory parameters compared with control yoghurt.
Result: In the present experiment the viability of L. acidophilus at 90 mins was 10.750± 0.553log10cfu/ml and the tolerance of L. acidophilus to 0.4 per cent of bile was significant. Microencapuslation of L. acidophilus was attempted by mixing carrot juice at 50 per cent to the alginate beads, thus imparting an appealing orange colour to the beads. The yoghurt thus prepared using microencapuslated L. acidophilus had an acidity of 0.7 ± 0.and total solid of 21.35 per cent on dry matter basis. Sensory analysis revealed maximum overall acceptability for carrot juice admixed microencapsulated probiotic-enriched yoghurt.

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REFERENCES

AOAC, (2000). Method 942.15.Acidity (titratable) of fruit products. Official Methods of Analyses of the Associate of Official Analytical Chemists (17ed.) AOAC,Washington D.C., USA.
Association of Official Analytical Chemists, (2006). Official methods of analysis, 18th Edn.2005, Current revision 1, (2006). Published by AOAC International, Suite 500, 481 North Frederick avenue, Gaithersburg, Maryland 20877-2417, USA.
Bansode, V., Pawar, D., Satpute, P.T., Zakiruddin, M., Jadhao, A.S. and Machewad, G.M. (2012). Studies on Effect of InitialpH, Storage Temperature and Preservatives on Shelf Life of Yoghurt. Indian Journal of Dairy Science. 65 (6).
De, Sukumar. (1980). Outlines of Dairy Technology. Oxford University Press, India.
FAO/WHO Working group Report. (2002). Guidelines for the evaluation of probiotics in food. London, Ontario, Canada
Garriga, M., Pascual, M., Montford, J.M and Hugas, M.(1998). Selection of Lactobacilli for chicken probiotic adjuncts. Journal of Applied. Microbiology. 84: 125-132.
Gibson, G.R. and Roberfroid, M.B. (1998). Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. Journal of Nutrition.125:1410-1412.
Gilliland, S.E. (1979). Beneficial interrelationships between certain microorganisms and humans: candidate microorganisms for use as dietary adjuncts. Journal of Food Protection.42: 164-167.
Goldin, B. R. and. Gorbach, S. L. (1992). Probiotics for humans. In: Probiotics. [R. Fuller, (ed)], Champmanand Hall, London. Pages 355-376
Indian Council of Medical Research–Department Biotechnology. (2011). Guidelines for Evaluation of Probiotics in Food, Published by Indian Council of Medical Research, New Delhi.
Jaya Prasad, Harsharanjit Gill, John Smart and Gopal, P.K. (1999). Selection and characterization of Lactobacillus and Bifidobacterium strains for use as probiotics.International Dairy Journal.8: 993-1002.
Jothi sri, Surya prabha, Seethadevi and Muthuprasanna. (2012). Microencapsulation: A review International Journal of Pharmacology and Bio Science.
Joseph P. Salji and Anwar, A. (1983). Effect of Initial Acidity of Plain Yogurt on Acidity Changes during Refrigerated Storage Journal of Food Science Volume. 48(1): 258-259.
Krasaekoopt, W.,Bhandari, B and Deeth H. (2003).Evaluation of encapsulation techniques of probiotics for yoghurt. International Dairy Journal. 13(1): 3-13.
McLauchalan, G., Fullarton, G.M., Crean, G.P.and McColl, K.E. (1998). Comparison of gastric body and antral pH: a 24 hour ambulatory study in healthy volunteers. Gut. 30: 573-578.
Olugbuyiro and Joy E. Oseh (2011). Physico-chemical and Sensory Evaluation of Market Yoghurt in Nigeria. Pakistan Journal of Nutrition 10 (10): 914-918, ISSN 1680-5194.
Samona, A. and Robinson, R.K. (1994). Eûect of yogurt cultures on the survival of bifidobacteria in fermented milks. Journal of the Society of Dairy Technology. 47: 58-60.
Snedecor, G.W. and Cochran, W.G. (1980). Statistical analytical methods. Oxford and IBM Publishers Co., New Delhi, India.
Zinedine, A. and Faid, M. (2007). Isolation and characterization of strains of Bifidobacteria with probiotic properties. World J. Dairy and Food Science. 2(1): 28-34.

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Incorporation of Microencapsulated Probiotic Lactic Acid Bacteria in Yoghurt

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