<div style="text-align: justify;"><strong>Background:</strong> The fertilization success and seed quality of Labeo rohita from different locations show wide variation. The important reason for these variations is sperm quality. Spermatological parameters are considered to be the deciding factors of sperm quality. The present study envisages delineating the strain-specific variations in spermatological parameters and seminal plasma composition of L. rohita from three different locations (Manimuthar, Thanjavur and Mettur) of Tamil Nadu.&nbsp;<br /><strong>Methods:</strong> In the present study conducted during 2019, milt samples were collected from three different strains of Labeo rohita. The samples were analyzed for the spermatological parameters such as pH, volume, sperm density, spermatozoa motility and seminal plasma composition (Na+, K+, Ca2+, Mg2+, Cl-) following standard protocols and the statistical comparisons were made.<br /><strong>Result: </strong>Among the strains, the spermatozoa of Thanjavur strain exhibited best performance with highest motility duration (82.33&plusmn;6.03 s) (p&lt;0.025) and sperm density (2.526&plusmn;0.25 &times; 109 cells/ml). With regard to seminal plasma composition, the concentration of Na+ (113.64&plusmn;4.97 mmol/L) and K+ (149.91&plusmn;1.67 mmol/L) was the highest in Thanjavur strain. Mettur strain exhibited highest Ca2+ (1.24&plusmn;0.06 mmol/L) content (p&lt;0.01) besides its lowest Na+ concentration (51.28&plusmn;3.52 mmol/L). The results revealed a significant correlation between ionic composition of seminal plasma and sperm motility. The increased motility duration in Thanjavur strain was due to increased K+ concentration. A synergistic effect of ions in controlling sperm motility was also observed as the higher Ca2+ concentration could sustain lowered Na+ concentration in Mettur strain.</div>

<div style="text-align: justify;">Comparative Study on Spermatological Parameters and Seminal Plasma Composition of <em>Labeo rohita</em> Strains from Tamil Nadu, India</div>

<div style="text-align: justify;">Taking into account that punicalagin (PUN) is a very powerful antioxidant; the current study evaluated the potential positive effects of oral PUN on spermatologic parameters of male New Zealand White rabbits. A total of 24 male bucks was housed individually and trained for semen collection. After the training for 2 wk, rabbits were assigned into 4 groups and received daily gavages of 0, 1, 2, and 10 mg/kg PUN in tap water for 9 wk. Semen was collected weekly from each rabbit and samples at d 1 and 63 of the experiment were analyzed separately. Initial values (ejaculate volume, ejaculate weight, ejaculate pH, sperm concentration, percent progressive motility, and seminal plasma protein levels) tested at d 1 were similar among the groups. &nbsp;There were also no differences in ejaculate volume, ejaculate weight, ejaculate pH and seminal plasma protein concentrations at the end of the experiment. Libido and reproductive organ weights were not affected by the treatments.However, sperm concentrations (P&lt;0.01) and percent progressive motility (P&lt;0.04) were significantly improved for bucks in 2 and 10 mg/kg PUN groups. Thus, the current study suggested that as low as 2 mg/kg/day PUN can be beneficial for spermatogenesis and motility in male rabbits.</div>

Comparative Study on Spermatological Parameters and Seminal Plasma Composition of Labeo rohita Strains from Tamil Nadu, India

Punicalagin is beneficial for spermatogenesis in male New Zealand White rabbits