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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 4 (april 2019) : 457-460,   Doi: 10.18805/ijar.B-915

Punicalagin is beneficial for spermatogenesis in male New Zealand White rabbits

O
Ozlem Yildiz-Gulay
M
Mehmet Sukru Gulay
1Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Physiology, Istiklal Yerleskesi, Burdur-15030, Turkey.
Cite article:- Yildiz-Gulay Ozlem, Gulay Sukru Mehmet (2018). Punicalagin is beneficial for spermatogenesis in male New Zealand White rabbits. Indian Journal of Animal Research. 53(4): 457-460. doi: 10.18805/ijar.B-915.

ABSTRACT

Taking into account that punicalagin (PUN) is a very powerful antioxidant; the current study evaluated the potential positive effects of oral PUN on spermatologic parameters of male New Zealand White rabbits. A total of 24 male bucks was housed individually and trained for semen collection. After the training for 2 wk, rabbits were assigned into 4 groups and received daily gavages of 0, 1, 2, and 10 mg/kg PUN in tap water for 9 wk. Semen was collected weekly from each rabbit and samples at d 1 and 63 of the experiment were analyzed separately. Initial values (ejaculate volume, ejaculate weight, ejaculate pH, sperm concentration, percent progressive motility, and seminal plasma protein levels) tested at d 1 were similar among the groups.  There were also no differences in ejaculate volume, ejaculate weight, ejaculate pH and seminal plasma protein concentrations at the end of the experiment. Libido and reproductive organ weights were not affected by the treatments.However, sperm concentrations (P<0.01) and percent progressive motility (P<0.04) were significantly improved for bucks in 2 and 10 mg/kg PUN groups. Thus, the current study suggested that as low as 2 mg/kg/day PUN can be beneficial for spermatogenesis and motility in male rabbits.

REFERENCES

  1. Acharya, U.R., Rathore, R.M., Mishra, M. (2003). Role of vitamin C on lead acetate induced spermatogenesis in swiss mice. Environmental Toxicology Pharmacology, 13: 9-14.
  2. Al-Olayan, E.M., El-Khadragy, M.F., Metwally, D.M., Abdel Moneim, A.E. (2014). Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats. BMC Complementary and Alternative Medicine, 14:164-172.
  3. Aloqbi, A., Omar, U., Yousr, M., Grace, M., Lila, M.A., Howell, N. (2016). Antioxidant activity of pomegranate juice and punicalagin. Natural Science, 8:235-246.
  4. Ata, A., Hatipoglu, F.S., Yildiz-Gulay, O., Gulay, M.S. (2007). Protective role of ascorbic acid on subacute sperm toxicity in male New Zealand white rabbits treated with endosulfan. Drug and Chemical Toxicology, 30: 181-195.
  5. Campos, A.C.N., Gadelha, C.R.F., Guerreiro, M.E.F., Pereira, E.S., Lima, I.C.S., Linard, M.A.B., Meneses, H.M., Castelo-Branco, K.F., Estevam, F.N.L. (2014). Male rabbit reproductive Physiology. Standard Research Journal of Agricultural Sciences, 8:120-128.
  6. Cerda, B.,Ceron, J.J., Tomás-Barberán, F.A., Espín, J.C. (2003). Repeated oral administration of high doses of the pomegranate ellagitannin punicalagin to rats for 37 days is not toxic. Journal of Agricultural and Food Chemistry, 51: 3493-3501.
  7. Chandra, G., Aggarwal, A., Singh, A., Singh, A.K., Kumar, M., Kushwaha, R., Singh, Y. K. (2012). Oxidative stress in sperm biology- A review. Agricultural Reviews, 33 (1): 54 - 61
  8. Coballase-Urrutia, E., Pedraza-Chaverri, J., Cardenas-Rodriguez, N., Huerta-Gertrudis, B et al.,(2011). Hepatoprotective effect of tonic and methanolic extracts of Heterotheca inuloides against CCl(4)-induced toxicity in rats. Exp Toxicol Pathol, 63:363–370.
  9. Fedder, M.D., Jakobsen, H.B., Giversen, I., Christensen, L.P., Parner, E.T., Fedder, J. (2014). An extract of pomegranate fruit and galangal rhizome increases the numbers of motile sperm: A prospective, randomized, controlled, double-blinded trial, PLOS ONE, 9: 1-9.
  10. Foote, R.H. (2002). Effects of metronidazole, ipronidazole, and dibromochloropropane on rabbit and human sperm motility and fertility. Reproductive Toxicology, 16: 749-755.
  11. Gil, M.I., Tomás-Barberán, F.A., Hess-Pierce, B., Holcroft, D.M., Kader, A.A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48: 4581-4589.
  12. Jean-Gilles, D., Li, L., Vaidyanathan, V.G., King, R., Cho, B., Worthen, D.R., Chichester, C.O., Seeram N.P. (2013). Inhibitory effects of polyphenol punicalagin on type-II collagen degradation in vitro and inflammation in vivo. Chemico-Biological Interactions, 205: 90-99.
  13. Leiva, K.P., Rubio, J., Peralta, F., Gonzales, G.F. (2011). Effect of Punicagranatum (pomegranate) on sperm production in male rats treated with lead acetate. Toxicology Mechanisms and Methods, 21: 495-502.
  14. Lin, C.C., Hsu, Y.F., Lin, T.C. (1999). Effects of punicalagin and punicalin on carrageenan-induced inflammation in rats. The American Journal of Chinese Medicine, 27: 371-376. 
  15. Machado, T.D., Leal, I.C.R., Amaral, A.C.F., dosSantos, K.R.N., da Silva, M.G., Kuster, R.M. (2002). Antimicrobial ellagitannin of Punica granatum fruits. Journal of the Brazilian Chemical Society, 13: 606-610.
  16. Mishra, M. and Acharya, U.R. (2004). Protective action of vitamins on the spermatogenesis in lead-treated Swiss mice. Journal of Trace Elements in Medicine and Biology, 18: 173–178.
  17. Nagappa, A.N., Thakurdesai, P.A., Venkat Rao, N., Singh, J. (2003). Antidiabetic activity of Terminalia catappa Linn fruits. Journal of Ethnopharmacology, 88: 45-50.
  18. Perumal, P. and Rajkhowa, C. (2015). Effect of addition of pomegranate (Punica granatum) juice on the liquid storage (5oc) of mithun (Bos frontalis) semen. Indian Journal of Animal Research, 49 (4): 470-473.
  19. Silva, L.P., de Angelis, C.D., Bonamin, F., Kushima, H., José Mininel, F., Campaner Dos Santos, L., et al (2015). Terminalia catappa L.: a medicinal plant from the Caribbean pharmacopeia with anti-Helicobacter pylori and antiulcer action in experimental rodent models. Journal of Ethnopharmacology, 159: 285-295.
  20. Rao, V.M. and Sharma, N.S.P. (2001). Protective effect of vitamin E against mercuric chloride reproductive toxicity in male mice. Reproductive Toxicology, 15: 705–712.
  21. Ramachandran, N. and Singh, N.P. (2017). Effect of management systems and seasons on sperm abnormalities in Jamunapari bucks semen. Indian Journal of Animal Research, 51 (6): 1138-1143.
  22. Sato, M. and Ishikawa, A. (2004). Room temperature storage of mouse epididymal spermatozoa: exploration of factors affecting sperm survival. Theriogenology, 61: 1455–1469.
  23. Türk, G., Sönmez, M., Aydin, M., Yüce, A., Gür, S., Yüksel, M., Aksu, and E.H., Aksoy, H. (2008). Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clinical Nutrition, 27: 289-296.
  24. Yaidikar, L., Byna, B., Thakur, S.R. (2014). Neuroprotective effect of Punicalagin against Cerebral Ischemia Reperfusion-induced Oxidative Brain Injury in rats. Journal of Stroke and Cerebrovascular Diseases, 23: 2869-2878.
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 4 (april 2019) : 457-460,   Doi: 10.18805/ijar.B-915

    Punicalagin is beneficial for spermatogenesis in male New Zealand White rabbits

    O
    Ozlem Yildiz-Gulay
    M
    Mehmet Sukru Gulay
    1Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Physiology, Istiklal Yerleskesi, Burdur-15030, Turkey.
    Cite article:- Yildiz-Gulay Ozlem, Gulay Sukru Mehmet (2018). Punicalagin is beneficial for spermatogenesis in male New Zealand White rabbits. Indian Journal of Animal Research. 53(4): 457-460. doi: 10.18805/ijar.B-915.

    ABSTRACT

    Taking into account that punicalagin (PUN) is a very powerful antioxidant; the current study evaluated the potential positive effects of oral PUN on spermatologic parameters of male New Zealand White rabbits. A total of 24 male bucks was housed individually and trained for semen collection. After the training for 2 wk, rabbits were assigned into 4 groups and received daily gavages of 0, 1, 2, and 10 mg/kg PUN in tap water for 9 wk. Semen was collected weekly from each rabbit and samples at d 1 and 63 of the experiment were analyzed separately. Initial values (ejaculate volume, ejaculate weight, ejaculate pH, sperm concentration, percent progressive motility, and seminal plasma protein levels) tested at d 1 were similar among the groups.  There were also no differences in ejaculate volume, ejaculate weight, ejaculate pH and seminal plasma protein concentrations at the end of the experiment. Libido and reproductive organ weights were not affected by the treatments.However, sperm concentrations (P<0.01) and percent progressive motility (P<0.04) were significantly improved for bucks in 2 and 10 mg/kg PUN groups. Thus, the current study suggested that as low as 2 mg/kg/day PUN can be beneficial for spermatogenesis and motility in male rabbits.

    REFERENCES

    1. Acharya, U.R., Rathore, R.M., Mishra, M. (2003). Role of vitamin C on lead acetate induced spermatogenesis in swiss mice. Environmental Toxicology Pharmacology, 13: 9-14.
    2. Al-Olayan, E.M., El-Khadragy, M.F., Metwally, D.M., Abdel Moneim, A.E. (2014). Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats. BMC Complementary and Alternative Medicine, 14:164-172.
    3. Aloqbi, A., Omar, U., Yousr, M., Grace, M., Lila, M.A., Howell, N. (2016). Antioxidant activity of pomegranate juice and punicalagin. Natural Science, 8:235-246.
    4. Ata, A., Hatipoglu, F.S., Yildiz-Gulay, O., Gulay, M.S. (2007). Protective role of ascorbic acid on subacute sperm toxicity in male New Zealand white rabbits treated with endosulfan. Drug and Chemical Toxicology, 30: 181-195.
    5. Campos, A.C.N., Gadelha, C.R.F., Guerreiro, M.E.F., Pereira, E.S., Lima, I.C.S., Linard, M.A.B., Meneses, H.M., Castelo-Branco, K.F., Estevam, F.N.L. (2014). Male rabbit reproductive Physiology. Standard Research Journal of Agricultural Sciences, 8:120-128.
    6. Cerda, B.,Ceron, J.J., Tomás-Barberán, F.A., Espín, J.C. (2003). Repeated oral administration of high doses of the pomegranate ellagitannin punicalagin to rats for 37 days is not toxic. Journal of Agricultural and Food Chemistry, 51: 3493-3501.
    7. Chandra, G., Aggarwal, A., Singh, A., Singh, A.K., Kumar, M., Kushwaha, R., Singh, Y. K. (2012). Oxidative stress in sperm biology- A review. Agricultural Reviews, 33 (1): 54 - 61
    8. Coballase-Urrutia, E., Pedraza-Chaverri, J., Cardenas-Rodriguez, N., Huerta-Gertrudis, B et al.,(2011). Hepatoprotective effect of tonic and methanolic extracts of Heterotheca inuloides against CCl(4)-induced toxicity in rats. Exp Toxicol Pathol, 63:363–370.
    9. Fedder, M.D., Jakobsen, H.B., Giversen, I., Christensen, L.P., Parner, E.T., Fedder, J. (2014). An extract of pomegranate fruit and galangal rhizome increases the numbers of motile sperm: A prospective, randomized, controlled, double-blinded trial, PLOS ONE, 9: 1-9.
    10. Foote, R.H. (2002). Effects of metronidazole, ipronidazole, and dibromochloropropane on rabbit and human sperm motility and fertility. Reproductive Toxicology, 16: 749-755.
    11. Gil, M.I., Tomás-Barberán, F.A., Hess-Pierce, B., Holcroft, D.M., Kader, A.A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48: 4581-4589.
    12. Jean-Gilles, D., Li, L., Vaidyanathan, V.G., King, R., Cho, B., Worthen, D.R., Chichester, C.O., Seeram N.P. (2013). Inhibitory effects of polyphenol punicalagin on type-II collagen degradation in vitro and inflammation in vivo. Chemico-Biological Interactions, 205: 90-99.
    13. Leiva, K.P., Rubio, J., Peralta, F., Gonzales, G.F. (2011). Effect of Punicagranatum (pomegranate) on sperm production in male rats treated with lead acetate. Toxicology Mechanisms and Methods, 21: 495-502.
    14. Lin, C.C., Hsu, Y.F., Lin, T.C. (1999). Effects of punicalagin and punicalin on carrageenan-induced inflammation in rats. The American Journal of Chinese Medicine, 27: 371-376. 
    15. Machado, T.D., Leal, I.C.R., Amaral, A.C.F., dosSantos, K.R.N., da Silva, M.G., Kuster, R.M. (2002). Antimicrobial ellagitannin of Punica granatum fruits. Journal of the Brazilian Chemical Society, 13: 606-610.
    16. Mishra, M. and Acharya, U.R. (2004). Protective action of vitamins on the spermatogenesis in lead-treated Swiss mice. Journal of Trace Elements in Medicine and Biology, 18: 173–178.
    17. Nagappa, A.N., Thakurdesai, P.A., Venkat Rao, N., Singh, J. (2003). Antidiabetic activity of Terminalia catappa Linn fruits. Journal of Ethnopharmacology, 88: 45-50.
    18. Perumal, P. and Rajkhowa, C. (2015). Effect of addition of pomegranate (Punica granatum) juice on the liquid storage (5oc) of mithun (Bos frontalis) semen. Indian Journal of Animal Research, 49 (4): 470-473.
    19. Silva, L.P., de Angelis, C.D., Bonamin, F., Kushima, H., José Mininel, F., Campaner Dos Santos, L., et al (2015). Terminalia catappa L.: a medicinal plant from the Caribbean pharmacopeia with anti-Helicobacter pylori and antiulcer action in experimental rodent models. Journal of Ethnopharmacology, 159: 285-295.
    20. Rao, V.M. and Sharma, N.S.P. (2001). Protective effect of vitamin E against mercuric chloride reproductive toxicity in male mice. Reproductive Toxicology, 15: 705–712.
    21. Ramachandran, N. and Singh, N.P. (2017). Effect of management systems and seasons on sperm abnormalities in Jamunapari bucks semen. Indian Journal of Animal Research, 51 (6): 1138-1143.
    22. Sato, M. and Ishikawa, A. (2004). Room temperature storage of mouse epididymal spermatozoa: exploration of factors affecting sperm survival. Theriogenology, 61: 1455–1469.
    23. Türk, G., Sönmez, M., Aydin, M., Yüce, A., Gür, S., Yüksel, M., Aksu, and E.H., Aksoy, H. (2008). Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clinical Nutrition, 27: 289-296.
    24. Yaidikar, L., Byna, B., Thakur, S.R. (2014). Neuroprotective effect of Punicalagin against Cerebral Ischemia Reperfusion-induced Oxidative Brain Injury in rats. Journal of Stroke and Cerebrovascular Diseases, 23: 2869-2878.
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