<div style="text-align: justify;">DNA was extracted from sarcocysts (visible on naked eye examination) collected from 168 buffaloes belonging to Mumbai (60), Hyderabad (54) and Kolkata (54) cities of India. &nbsp;They were subjected to PCR assay using 18S rRNA gene primer. All the PCR amplicons of about 900 bp were subjected to restriction enzyme digestion with four different restriction enzymes (BslI, DraI, FokI and RsaI). PCR amplicons showed two different patterns (Pattern A and Pattern B) on RFLP. &nbsp;Twenty one PCR products from Pattern A and one PCR product from Pattern B were subjected to DNA sequencing. S. fusiformis and S. taeniata were identified from Pattern A and S. buffalonis was identified from Pattern B on sequencing.&nbsp;</div>

Molecular approaches in species identification of Sarcocysts in Indian buffalo meat&nbsp;

<div><strong>Background:</strong> A slaughter house study helps in the assessment of the disease status of herds and contain valuable informations about the incidence and epidemiology of animal diseases. Abattoir study also plays a significant role to know about the extent of exposure of the public to certain zoonotic diseases and helps in estimation of the financial losses due to condemnation of affected organs.&nbsp;Even in India, there is a lack of data from a proper slaughterhouse study of cows in Mizoram to determine the prevalence of pathological lesions affecting the organs and corpses.<br />
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<strong>Methods: </strong>Total of 90 samples of each organ i.e. liver, lungs, kidneys, heart, spleen and 35 intestine samples were collected during the period from March 2019 to March 2020 for the present study from the Govt. slaughterhouse, Aizawl, Mizoram. Representative tissue samples were collected in 10% neutral buffered formalin (NBF) for further histopathological analysis. Fixed tissue samples processed through paraffin embedding technique and sections were cut at a 4-5 m thickness, stained with H and E and special staining like Masson&rsquo;s trichrome, Luna-Ishak, Von Kossa and Perl&rsquo;s.&nbsp;<br />
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<strong>Result: </strong>Among all the organs studied, liver (65.55%) showed the highest prevalence of pathological lesions followed by intestine (42.86%), lungs (38.88), kidneys (35.55%), heart (31.11%) and spleen (20%). Circulatory disturbances were the most common pathological lesion encountered in all the organs. The major pathological condition encountered in liver was hepatitis and fibrosis. Interstitial pneumonia was more prevalent in the lungs than bronchopneumonia. Glomerulonephritis was more common in the kidney than tubulo-interstitial nephritis. In the heart, Sarcocyst was more prevalent than myocarditis.</div>


Molecular approaches in species identification of Sarcocysts in Indian buffalo meat

Pathological Studies on Specimens Collected from Slaughtered Bovines in Aizawl District of Mizoram