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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 3 (march 2019) : 388-393,   Doi: 10.18805/ijar.B-3519

Molecular approaches in species identification of Sarcocysts in Indian buffalo meat 

C
C. Ramakrishna
S
S. Vaithiyanathan
M
M. Muthukumar
L
L.R. Chatlod
P
P. Lavanya
V
V.V. Kulkarni
1ICAR – National Research Centre on Meat, Hyderabad – 500 092, Telangana, India.
Cite article:- Ramakrishna C., Vaithiyanathan S., Muthukumar M., Chatlod L.R., Lavanya P., Kulkarni V.V. (2018). Molecular approaches in species identification of Sarcocysts in Indian buffalo meat. Indian Journal of Animal Research. 53(3): 388-393. doi: 10.18805/ijar.B-3519.

ABSTRACT

DNA was extracted from sarcocysts (visible on naked eye examination) collected from 168 buffaloes belonging to Mumbai (60), Hyderabad (54) and Kolkata (54) cities of India.  They were subjected to PCR assay using 18S rRNA gene primer. All the PCR amplicons of about 900 bp were subjected to restriction enzyme digestion with four different restriction enzymes (BslI, DraI, FokI and RsaI). PCR amplicons showed two different patterns (Pattern A and Pattern B) on RFLP.  Twenty one PCR products from Pattern A and one PCR product from Pattern B were subjected to DNA sequencing. S. fusiformis and S. taeniata were identified from Pattern A and S. buffalonis was identified from Pattern B on sequencing. 

REFERENCES

  1. Dissanaike A, S. and Kan, S. P. (1978) Studies on Sarcocystis in Malasia. 1. Sarcocystis levinei n. sp. From the water buffalo Bubalus bubalis. Zeits Parasit. 55: 127 – 128.
  2. Dubey, J. P., Speer, C. A. and Fayer, R. (1989) Sarcocystosis of animals and man. C. A. C. Press Inc. Boca, Raton, FL. Pp 105-145. 
  3. El-Dakhly, K. M., El-Nesr, K. A., El – Nahass, E., Hirata, A., Sakai, H. and Yanai, T. (2011) Prevalence and distribution patterns of Sarcocystis species in buffaloes in Beni – Suef, Egypt. Tropical Animal Health and Production, Epub ahead of print.
  4. El-Morsy, A. M. (2010) Studies on Sarcocystis species infecting water buffaloes in Egypt. Unpublished M.V.Sc. Thesis, Fac. Vet. Med. Kafrelsheikh Univ.
  5. El-Seify M., El-Morsey,A., Hilali, M., Zayed, A. El-Dakhly K., Haridy, M., Sakai, H. and Yanai, T. (2014) Molecular characterization of Sarcocystis fusiformis and Sarcocystis buffalonis infecting water buffaloes (Bubalus bubalis) from Egypt. American Journal of Animal and Veterinary Sciences. 9: 95-104. 
  6. Fayer, R. (2004) Sarcocystis spp. In human infections. Clinical Microbiology Reviews. 17: 894 – 902.
  7. Ghaffar, F., Hilali, M and Scholtyseck, E. (1978) Ultrastructural studies of Sarcocystis fusiformis (Railliet, 1987) infecting Indian water buffalo (Bubalus bubalis) of Egypt. Tropenmed Parasitol. 29: 283 294.
  8. Gjerde, Bjorn (2014) Morphological and molecular characteristics of four Sarcocystis spp. In Canadian moose (Alces alces), including Sarcocystis taeniata n. sp. Parasitology Research. 113: 1591.
  9. Huong, L.T.T. (1999) Prevalence of Sarcocystis spp. In water buffaloes in Vietnam. Vet. Parasitol. 86: 33 – 39.
  10. Huong, L. T. T., Dubey, J. P. and Uggla, A. (1995). Prevalence and identity of Sarcocystis in water buffaloes and cattle slaughtered at some abattoirs in Ho Chi Minh City. Proc. 1st Conf. Vet. Med. Ani. Husb., University of Agriculture and Forestry, Ho Chi Minh City, Vietnam, I: 88 – 97. 
  11. Huong, L.T.T., Dubey, J. P, Nikkila, T. and Uggla, A. (1997). Sarcocystis buffalonis n. sp. (Protozoa: Sarcocystidae) from the water buffalo (Bubalus bubalis) in Vietnam. J. Parasitol. 83: 471 – 474.
  12. Juyal, P. D. and Bhatia, B. B. (1989) Sarcocystosis: an emerging zoonosis. Indian Veterinary Medical Journal. 13: 66-9.
  13. Oryan, A., Sharifiyazdi, H, Khordadmehr, M and Larki, S. (2011). Characterization of Sarcocystis fusiformis based on sequencing and PCR – RFLP in water buffalo (Bubalus bubalis) in Iran. Parasitol. Res. 109: 1563 – 1570.
  14. Prakas, P. Butkauskas, D, Rudaityt, E. and Kutkien, L (2016) Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. From the Sika deer (Cervus Nippon) in Lithuania. Parasitol Res. 115: 3021-32.
  15. Retzlaff, N. and Weise, E. (1969) Sarkosporidien bein wasser buffel (Bubalus bubalis) in der turkey. Ber. Munch. Tierarztl. Wschr. 82: 283 – 286.
  16. Salah, A., Abu – Elwafa, Ibrahim, E. A. Anbas and Moustafa A. Al-Araby (2015) Ultrastructure of Sarcocystis buffalonis (Huong et al., 1997) infecting water buffaloes (Bubalus bubalis) from Egypt. International Journal of Advanced Research. 3: 452 – 457.
  17. Xiao, B. N., Zhang, Y. S. and Yong, Z. F. (1988): A survey of Sarcosporidiosis in domestic animals. Chinese J. Vet. Med. 14: 12 – 14. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 3 (march 2019) : 388-393,   Doi: 10.18805/ijar.B-3519

    Molecular approaches in species identification of Sarcocysts in Indian buffalo meat 

    C
    C. Ramakrishna
    S
    S. Vaithiyanathan
    M
    M. Muthukumar
    L
    L.R. Chatlod
    P
    P. Lavanya
    V
    V.V. Kulkarni
    1ICAR – National Research Centre on Meat, Hyderabad – 500 092, Telangana, India.
    Cite article:- Ramakrishna C., Vaithiyanathan S., Muthukumar M., Chatlod L.R., Lavanya P., Kulkarni V.V. (2018). Molecular approaches in species identification of Sarcocysts in Indian buffalo meat. Indian Journal of Animal Research. 53(3): 388-393. doi: 10.18805/ijar.B-3519.

    ABSTRACT

    DNA was extracted from sarcocysts (visible on naked eye examination) collected from 168 buffaloes belonging to Mumbai (60), Hyderabad (54) and Kolkata (54) cities of India.  They were subjected to PCR assay using 18S rRNA gene primer. All the PCR amplicons of about 900 bp were subjected to restriction enzyme digestion with four different restriction enzymes (BslI, DraI, FokI and RsaI). PCR amplicons showed two different patterns (Pattern A and Pattern B) on RFLP.  Twenty one PCR products from Pattern A and one PCR product from Pattern B were subjected to DNA sequencing. S. fusiformis and S. taeniata were identified from Pattern A and S. buffalonis was identified from Pattern B on sequencing. 

    REFERENCES

    1. Dissanaike A, S. and Kan, S. P. (1978) Studies on Sarcocystis in Malasia. 1. Sarcocystis levinei n. sp. From the water buffalo Bubalus bubalis. Zeits Parasit. 55: 127 – 128.
    2. Dubey, J. P., Speer, C. A. and Fayer, R. (1989) Sarcocystosis of animals and man. C. A. C. Press Inc. Boca, Raton, FL. Pp 105-145. 
    3. El-Dakhly, K. M., El-Nesr, K. A., El – Nahass, E., Hirata, A., Sakai, H. and Yanai, T. (2011) Prevalence and distribution patterns of Sarcocystis species in buffaloes in Beni – Suef, Egypt. Tropical Animal Health and Production, Epub ahead of print.
    4. El-Morsy, A. M. (2010) Studies on Sarcocystis species infecting water buffaloes in Egypt. Unpublished M.V.Sc. Thesis, Fac. Vet. Med. Kafrelsheikh Univ.
    5. El-Seify M., El-Morsey,A., Hilali, M., Zayed, A. El-Dakhly K., Haridy, M., Sakai, H. and Yanai, T. (2014) Molecular characterization of Sarcocystis fusiformis and Sarcocystis buffalonis infecting water buffaloes (Bubalus bubalis) from Egypt. American Journal of Animal and Veterinary Sciences. 9: 95-104. 
    6. Fayer, R. (2004) Sarcocystis spp. In human infections. Clinical Microbiology Reviews. 17: 894 – 902.
    7. Ghaffar, F., Hilali, M and Scholtyseck, E. (1978) Ultrastructural studies of Sarcocystis fusiformis (Railliet, 1987) infecting Indian water buffalo (Bubalus bubalis) of Egypt. Tropenmed Parasitol. 29: 283 294.
    8. Gjerde, Bjorn (2014) Morphological and molecular characteristics of four Sarcocystis spp. In Canadian moose (Alces alces), including Sarcocystis taeniata n. sp. Parasitology Research. 113: 1591.
    9. Huong, L.T.T. (1999) Prevalence of Sarcocystis spp. In water buffaloes in Vietnam. Vet. Parasitol. 86: 33 – 39.
    10. Huong, L. T. T., Dubey, J. P. and Uggla, A. (1995). Prevalence and identity of Sarcocystis in water buffaloes and cattle slaughtered at some abattoirs in Ho Chi Minh City. Proc. 1st Conf. Vet. Med. Ani. Husb., University of Agriculture and Forestry, Ho Chi Minh City, Vietnam, I: 88 – 97. 
    11. Huong, L.T.T., Dubey, J. P, Nikkila, T. and Uggla, A. (1997). Sarcocystis buffalonis n. sp. (Protozoa: Sarcocystidae) from the water buffalo (Bubalus bubalis) in Vietnam. J. Parasitol. 83: 471 – 474.
    12. Juyal, P. D. and Bhatia, B. B. (1989) Sarcocystosis: an emerging zoonosis. Indian Veterinary Medical Journal. 13: 66-9.
    13. Oryan, A., Sharifiyazdi, H, Khordadmehr, M and Larki, S. (2011). Characterization of Sarcocystis fusiformis based on sequencing and PCR – RFLP in water buffalo (Bubalus bubalis) in Iran. Parasitol. Res. 109: 1563 – 1570.
    14. Prakas, P. Butkauskas, D, Rudaityt, E. and Kutkien, L (2016) Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. From the Sika deer (Cervus Nippon) in Lithuania. Parasitol Res. 115: 3021-32.
    15. Retzlaff, N. and Weise, E. (1969) Sarkosporidien bein wasser buffel (Bubalus bubalis) in der turkey. Ber. Munch. Tierarztl. Wschr. 82: 283 – 286.
    16. Salah, A., Abu – Elwafa, Ibrahim, E. A. Anbas and Moustafa A. Al-Araby (2015) Ultrastructure of Sarcocystis buffalonis (Huong et al., 1997) infecting water buffaloes (Bubalus bubalis) from Egypt. International Journal of Advanced Research. 3: 452 – 457.
    17. Xiao, B. N., Zhang, Y. S. and Yong, Z. F. (1988): A survey of Sarcosporidiosis in domestic animals. Chinese J. Vet. Med. 14: 12 – 14. 
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