Background: The global rise of antimicrobial resistance (AMR) poses a serious threat to public health, emphasizing the urgent need for accurate, rapid and efficient detection methods. Conventional approaches for detecting AMR genes typically involve bacterial isolation followed by DNA extraction-procedures that are time-consuming, involve multiple steps and are not well-suited for routine screening. This study aimed to optimize a direct PCR method for the detection of AMR genes and to apply the standardized protocol for screening these genes in various sample types. 
 
Methods: A total of 150 samples (50 samples from each category) were collected from Chennai district, Tamil Nadu, India. The bacteria was isolated following standard protocols, DNA was extracted and PCR amplication was done to detect the presence of AMR genes. In the Direct PCR Master, PCR amplification was done directly from samples without the need for prior DNA extraction.&nbsp; 
 
Result: Escherichia coli was most prevalent in cloacal samples, followed by chicken meat and water, whereas Staphylococcus aureus was predominantly found in chicken meat, followed by water and cloacal samples. Regardless of the source, the genes tetA, sul1 and blaOXA-1 were consistently detected in E. coli, while tetA, sul1 and qnrA were most prevalent in S. aureus isolates. When comparing conventional culture dependent method with the direct PCR method, the latter showed a high concordance rate (&gt;76%) with the culture-dependent method, demonstrating strong sensitivity and specificity for most target genes. Additionally, the direct PCR approach offers several advantages, including reduced risk of contamination, a simplified workflow and shorter turnaround times. These benefits make it a promising alternative for rapid detection of antimicrobial resistance (AMR) genes, especially in resource-limited settings or during outbreak situations.

Rapid Detection of Antimicrobial Resistance (AMR) Genes by Direct PCR Method: A Comparative Study with Culture-dependent Method

<div style="text-align: justify;">Background: The genetic variability in a population is the raw material for selection, because the estimation of genetic parameters depends on the variability present within the population. The pedigree analysis is a method to assess population genetic variability. An increase in the level of inbreeding disturbs the production performance of the animals. Hence, it is essential to assess the effect of inbreeding on production performance of the animals at regular intervals. The present study was conducted on data of Bharat Merino (BM) sheep with twin objectives of evaluating the population structure by pedigree analysis and possible effect of inbreeding on lamb growth and heritability estimates.&nbsp; Methods: The study was conducted on data consisting of a total of 9688 pedigree records of BM sheep born from 1975 to 2018 (43 years), out of which 9050 formed population reference (with both the parents known). ENDOG ver 4.8 program was used to generate different measures of genetic diversity. General Linear Model of SPSS 25.0 was used to ascertain the effect of inbreeding (Fi) or change in inbreeding (&Auml;Fi) on the lamb live weights. Using animal model with software WOMBAT, single trait linear mixed model analyses were performed. The heritability estimates and breeding values were obtained by including or excluding the inbreeding coefficient in the model to observe how the estimates of heritability varied with inclusion or exclusion of the inbreeding coefficient.&nbsp; Result: Effective number of founders (fe) was 56, constituting 11.39% founders in the population reference, while the effective number of ancestors (fa) was 43.The genetic contribution of the 15 most influent ancestors explained 50% of the genetic variability in the dataset. The ratio fe/fa, representing the effect of population bottleneck, was 1.302. The average inbreeding coefficients for the whole pedigree was 2.36%, while it was 3.84% for inbred animals. It was found that the inbreeding coefficient (Fi) increased with the addition of each generation to the pedigree. The average relatedness coefficient was 4.53% between members of the population.The effect of individual inbreeding (Fi) or the change in inbreeding (DFi) was not significant on the lamb live weights, except the effect of individual inbreeding (Fi) on three-month body weight and average daily gain (0-3month) and of change in inbreeding (DFi) on three-month body weight. From the analysis of the pedigree data of Bharat Merino sheep, it was found that the most of the measures of genetic diversity were within acceptable limits and the pedigree data was reasonably well maintained. When inbreeding was accounted for in the model, there were reductions in h2 estimates as well as the estimates of breeding values for both 3WT and ADG1 and consequently there were changes in ranking of animals for both 3WT and ADG1.&nbsp;</div>

<div style="text-align: justify;">Genetic Variability of Bharat Merino Sheep Derived from Pedigree Information</div>

<div>Background: Loss of genetic diversity is allied with adverse incidents such as inbreeding depression in fitness related traits, addiction of favorable alleles and enhances fluctuation on selection response. Therefore, the study aims to monitor population structure and avoid unfavorable effects of inbreeding for sustainable improvement in breeding programme. 
 
Methods: Pedigree information on 3449 animals born from 1955-2018 was analyzed. The effect on inbreeding on Age at first calving (AFC), Service period (SP), Daughter pregnancy rate (DPR), Calving interval (CI) and pregnancy rate was seen by regression analysis. 
 
Result: The pedigree completeness index (PCI) were found to be (%) 92.32, 80.26, 65.22, 49.43% and 32.54 respectively from first to fifth generations. The estimated average inbreeding rate was 2.30% and average genetic diversity loss was 2.64%. Study also revealed significant effect of inbreeding on (AFC, CI and SP) with slightly increasing trends. Hence, it is suggested that more precise pedigree recording and planned mating strategies should be adopted to avoid negative effect of inbreeding in future generation.&nbsp;</div>

<div>In-depth Pedigree Analysis and Inbreeding Effect on Reproduction Traits in Sahiwal Nucleus Herd&nbsp;</div>

<div style="text-align: justify;">An experiment was conducted during 2014-2015 to standardize the seed testing method in Kabuli chickpea MNK-1 by number of seeds and number of germination papers. Among the different testing methods 25 seeds per replication recorded highest seed germination percentage (91.58%), less number of fresh un-germinated seeds (1.91%), abnormal seedlings (4.00%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2215 and 6863), among the number of germination papers used 2+1 &nbsp;as in between paper method(Bottom 2 and one paper above) recorded significantly higher germination percentage (92.12%), less number of fresh un-germinated seeds (1.41%), abnormal seedlings (3.66%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2463 and 6900), when compared to other. Irrespective of number of germination paper and seeds used however, interaction of 25 seeds per replication and 2+1 between paper method of seed germination testing showed significantly germination percentage (96.50%), less number of fresh un-germinated seeds (0.00%), abnormal seedlings (1.00%), diseased seeds (1.00%) and significantly higher seedling vigour index-I and II (2844 and 7792) followed by 50 seeds per replication.&nbsp;</div>

An improved method of seed germination testing in Kabuli chickpea

<div style="text-align: justify;">Black Soybean is highly nutritious legume of Uttarakhand but its hard seed coat reduces the nutrient availability to consumer. Attempt was made in this experiment to study effect of processing method on proximate composition of black soybean. Four Kilograms of black soybean was taken and subjected to three different processing methods like germination, roasting and cow urine treatment while one group was taken as control. Results indicated highly significant (Pd&rdquo;0.01) differences in dry matter, crude protein, ether extract, ash and crude fibre content of black soybean among different groups compared to control. All processing methods were effective in improving nutritional value of black soybean thereby increasing its utilization in foods and food formulations.</div>

Effect of processing method on nutritional quality of black soybean

Background: Direct wet seeding offers the advantage of faster and easier sowing, reduced labour and less drudgery, 7-10 days earlier crop maturity, more efficient water use and higher tolerance to water deficit, less methane emission, and often higher profit in areas with assured water supply. There is a possibility of intercropping green manures during early growth stage of rice with less interference on the crop growth, which also smothering the weed growth to enhance the productivity of wet direct seeded rice. 
 
Methods: A field experiment was conducted during Kharif, 2019 and 2020 at the Department of Rice, Tamil Nadu Agricultural University, Coimbatore, to study the time of sowing and method of sowing under wet direct seeded rice. The experimental trial was laid out in split plot design with three replications. Treatments comprising of time of sowing in main plot viz., normal sowing time in first fortnight of July and delayed sowing time in second fortnight of July. In sub-plot, method of sowing viz., broadcasting method, line sowing method, paddy drum seeder method, paddy + dhaincha drum seeder method and direct planting method.&nbsp; 
 
Result: The results revealed that, sowing of wet direct seeded rice during first fortnight of July month by using Paddy + Dhaincha drum seeder method recorded higher grain yield of 5707 kg/ha which resulted 33 per cent higher yield than that of broadcasting method.

Effect of Time and Method of Sowing in Wet Direct Seeded Rice

<div>Harvesting methods adopted for paddy crop are largely dependent on the land size of the farmers. Mechanized methods are completely implemented by the farmers of medium and large categories. On the other hand, the marginal and small farmers are struggling between manual and mechanized methods because of unavailability of implements that can suit to their land size an economic level. Cutting and threshing operations are performed separately which are responsible for more input cost and grain loss. Various methods of harvesting were studied and the loss of grain associated with the methods is emphasized. In manual method, before threshing, the cutting, collecting, bundling and transportation operation requires which consumes time, energy and cost and increase the grain loss significantly. In mechanized methods, the time, energy and input cost can be minimized. The manual crop cutting requires about 8 to 12 and 25 to 45 times, respectively, more man-hours per hectare compared to rotary blade cutter and vertical conveyor reaper. The field capacity of manual harvesting methods is 4 to 10 times less than the mechanized methods.</div>

Harvesting and Threshing Methods for Paddy-I: A Review

<div>Background: The present investigation is an attempt to study the effect of different harvesting and threshing methods on germination and seedling vigour of dhaincha. The use of a combine harvester to harvest dhaincha will be an effective alternative method that has not been widely tried. However, work on different methods of harvesting and threshing of dhaincha is limited. Hence, a study was conducted to evaluate the impact of various harvesting and threshing methods on germination and seedling vigour of dhaincha. 
 
Methods: This study was conducted at Anbil Dharmalingam Agricultural College and Research Institute, TNAU, Tiruchirappalli, Tamil Nadu. The dhaincha crop was harvested and threshed using four different methods viz., manual harvesting and manual threshing, manual harvesting and threshing by tractor treading, manual harvesting and mechanical threshing&nbsp;and harvesting and threshing by combine harvester. The resultant seeds were tested for mechanical damage and germination potential. The experiment was laid in completely randomized block design. Germination was tested by roll towel method using 100 seeds in four replications. Germination percentage, root and shoot length were measured in seven days after sowing from ten randomly selected seedlings in each replication. For the estimation of dry matter production, ten seedlings were selected at random and kept in a hot air oven maintained at 85oC for 24 hours after measuring their root and shoot length and vigour index was calculated. Mechanical damage to seeds was observed by ferric chloride test. Seed recovery per cent was calculated based on the 100 kgs of dhaincha seeds were cleaned and graded treatment wise using cleaner cum grader and the seeds retained on the bottom sieve were weighed and expressed as per cent of total quantity of seed. 
 
Result: The results revealed that the significant difference was found among the different harvesting and threshing methods. The seeds harvested and threshed by manual method recorded 85 per cent germination followed by seeds harvested manually and threshed by mechanical threshing (84 per cent) and seeds harvested manually and threshed by tractor treading (80.5 per cent). The lowest germination of 80.0 per cent was recorded by combine harvesting. From this study, it could be inferred that combine harvester is a modern method for harvesting of dhaincha that saves time and labour when compared to all other methods.</div>

Harvesting and Threshing Methods on Seed Quality of&nbsp;Dhaincha (Sesbania aculeata)

<div style="text-align:justify">
This review deals with the various traditional and recent methods of sire evaluation. A sire evaluation is a method of prediction of sire&rsquo;s next-generation produced by breeding with specified females and creating their records in a specific environment. Breeding worth of progeny tested sires are obtained through sire index method that assigns ranking to each sire based on their genetic merit. Numerous sire indices are broadly classified in two type&rsquo;s viz., indices which are purely meant for ranking purposes and those which, besides, provide an estimate of the breeding worth of each sire. Sires can be evaluated in single or multiple herds. The statistical equations represent sire evaluation as linear or non-linear methods. Numerous methods can be incorporated as the advances are made in sire evaluation based on data structures, breeding approaches and selection methods. There are various approaches of sire evaluation such as Least Squares Method (LSM), Simple Regressed Least Squares (SRLS), Best Linear Unbiased Prediction (BLUP) and Derivative-Free Restricted Maximum Likelihood (DFREML) for single as well as multiple trait models which can be used to derive genetic worth of an individual. An efficient method of sire evaluation shows minimum within sire variance or error variance.&nbsp;
</div>

Recent Advances in Sire Evaluation Methods: A Review

Background: Bael is an important indigenous fruit, which is rich in nutritional and health promoting factors. Development of value-added products from this fruit poses a problem as the fruit has a hard rind and is difficult to be removed by hand.&nbsp; 
 
Methods: The objective of this investigation was to evaluate the effect of different conditioning methods as normal water dipping (25-27&deg;C), chilling (10-11&deg;C), freezing (4-5&deg;C) and hot water dipping (90-95&deg;C) on firmness and other mechanical properties of raw and matured bael fruit, with a goal of devising some methods for easy removal of the rind. The fruits after conditioning were subjected to puncture test by universal testing machine. Distinct peaks were observed on the force-displacement traces which indicated the rupture force and firmness of the fruits. The changes observed in rupture force, deformation and firmness in conditioned samples were compared. 
 
Result: Rupture force measured for control sample (kept under ambient conditions) was 344.4&plusmn;27.13 N and was found to be lower than that of the conditioned samples. The minimum rupture force of 192.6&plusmn;14.41 N was observed in frozen sample. No significant difference in rupture force could be observed between the normal water dipped sample and chilled samples. Hot water dipped sample required a rupture force of 215.3&plusmn;29.2 N, which was not significantly (p&gt;0.05) less than those of other treatments but, the green color of the fruits degraded to brown. The change in other mechanical properties also remained similar. The results would be useful for preparing the raw bael fruit for further processing and value addition.

Rapid Detection of Antimicrobial Resistance (AMR) Genes by Direct PCR Method: A Comparative Study with Culture-dependent Method

Genetic Variability of Bharat Merino Sheep Derived from Pedigree Information

In-depth Pedigree Analysis and Inbreeding Effect on Reproduction Traits in Sahiwal Nucleus Herd

An improved method of seed germination testing in Kabuli chickpea

Effect of processing method on nutritional quality of black soybean

Effect of Time and Method of Sowing in Wet Direct Seeded Rice

Harvesting and Threshing Methods for Paddy-I: A Review

Harvesting and Threshing Methods on Seed Quality of Dhaincha (Sesbania aculeata)

Recent Advances in Sire Evaluation Methods: A Review

Effect of Conditioning Methods on Mechanical Properties of Raw Bael Fruit