Background: Phytotherapeutic approaches are becoming increasingly important for the treatment of various diseases and disorders. Therefore, the&nbsp;identification of plants with the desired bioactivity properties is important. The primary purpose of this study is to determine the phenolic composition of Lathyrus annuus, L. hierosolymitanus and L. hirsutus and reveal the bioactivities of extracts obtained using aerial parts of these species. 
 
Methods: Twelve phenolic compounds were investigated and their amounts were determined by HPLC analysis. For the detection of the bioactivities of species, antioxidant ability tests (DPPH, ABTS, RP) and enzyme inhibition assays were carried out. Pancreatic lipase and amylase were selected as target enzymes for the inhibition assays. 
 
Result: Eight of the&nbsp;investigated twelve phenolic compounds were detected in all species. The gallic acid and p-hydroxybenzoic acid contents of L. hirsutus were found to be in conspicuous amounts. The ethanol extracts of L. hirsutus and L. annuus and the water extract of L. hierosolymitanus showed the highest antioxidant activity in the DPPH, ABTS and RP assays, respectively. The lowest antioxidant property was shown by the acetone extract of L. annuus among all extracts. The acetone extract of L. hierosolymitanus and ethanol extract of L. annuus had the strongest inhibitory effects on amylase and lipase enzyme activities, respectively.

Antioxidant Properties and Enzyme Inhibitory Effects of Extracts of Three Lathyrus Species (Fabaceae) from Türkiye