Background: Energy is the important part of any life, the potential energy present in the tissue in the form of chemical energy. Presence of glycogen as glucose is source of energy for cell division, differentiation, delamination and maturation process as essential process in histogenesis of foetal organ. Glycosaminoglycans, also known as mucopolysaccharides, are long chains of sugar occurs in different proportion in different tissue and their distribution in compartment of ruminant stomach varied in foetus of different CRL. Copmartment of ruminant stomach abomasum , omasum, reticulum and rumen was contributed grossly in varied proportion from initial stage to full term of prenatal stage. In initial stage rumen is largest compartment with advancement of development omasum is largest compartment&nbsp; followed by reticulum and abomasum at full term.&nbsp;As per morphology and histology of varied compartment there could be probability of varied intensity of histochemical reaction in different layer of tissue as per their ebryogenesis.&nbsp;Kosali cattle is registered 36th breed of India. Kosali the native breed of Chhattisgarh, known for its compact body, disease tolerance and well maintained in agricultural waste. More than total 60% Kosali cattle are concentrated in the central plains of the C.G. state. There is paucity of Cited literature, literally no scientific documentation regarding presence and distribution of glycogen and mucopolysaccharides in rumen, reticulum, omasum and abomasum of prenatal Kosali cattle. In view importance of Kosali cattle concern with livelihood of farmers in Chhattisgarh, varied degree of development of compartment of stomach and paucity of literature regarding intensity of histochemical reaction&nbsp; from initial to final term of prenatal stage present research was undertaken. 
 
Methods: In light of above background in twenty four Kosali foeti were collected and on the basis of CR length categorizedin to four groups with six foeti in each group i.e. Group I (below 20 cm CRL), Group II (Above 20 up to 40 cm CRL), Group III (Above 40 up to 60 cm CRL) and group IV (Above 60 cm CRL) , tissue samples ofrumen, reticulum, omasum and abomasum were collected and processed&nbsp; for study of glycogen and mucopolysaccharide by PAS and&nbsp; AB-PAS method respectively. 
 
Result: Apparently in group I PAS&nbsp; activity was either not observed&nbsp; as in reticulum or very mild in rumen, omasum and abomasum and was greater in abomasum. In epithelium, propria submucosa and serosa PAS activity increased slowly mostly from mild to moderate while in glands reaction was intense. However in tunica muscularis of rumen reaction decreased from group III to IV , in omasal tunica muscularis and omasal laminae from group II to IV. In group I PAS activity was higher in pyloric region than cardiac stomach. AB-PAS activity for neutral mucopolysaccharide was&nbsp; observed in epithelium and tunica muscularis of rumen, reticulum, omasum and abomasum in all groups, mixed mild activity for both neutral and acid mucopolysaccharide in propria submucosa in group I and II. In fundic region in group III and IV in glands intense acid mucopolysaccharide reaction was seen in patches in forerunner parietal cells.

Prenatal Histochemical Study of Stomach in Kosali Cattle

Background: Kosali cattle are a native breed from Chhattisgarh, India, primarily raised in rural areas on agricultural residues. However, there is limited information about the prenatal development of their thymus. Therefore, current study was conducted to understand the prenatal morphohistogenesis of thymus in Kosali breed of cattle. 
 
Methods: The present investigation involves 24 Kosali foetuses for the prenatal gross morphometric development of the thymus and categorized on the basis of crown-rump length (CRL) into four groups: Group I (CRL &lt;20 cm), Group II (20-40 cm), Group III&nbsp; (40-60 cm) and Group IV (&gt;60 cm). A midventral cervical incision from the larynx to the thoracic inlet was made to expose the thymus for in situ examination of its topography and morphology. Thymus samples were collected and processed for Hematoxylin and Eosin, Van Gieson&rsquo;s, Verhoeff&rsquo;s and Gomori&rsquo;s staining for histological changes. 
 
Result: In Group I, the thymus had paired cervical parts and one unpaired thoracic part, with the right limb extending to the middle thyroid cartilage and the left to the cricoid cartilage. By Groups II to IV, the left limb shifted laterally. The thymus color changed from cream to pink and while lobes became more distinct, their number decreased and size increased. The capsule thickened and became more vascular. The stroma contained interlobar and interlobular septa that thinned over groups and the number of lobules per field decreased significantly, indicating involution of the thymus and functional adaptations with age. These observations suggest that the thymus undergoes progressive involution with age. The structural and vascular changes seem to reflect the functional adaptations that occur during development. 
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Prenatal Histochemical Study of Stomach in Kosali Cattle

Prenatal Morphohistogenesis of Thymus in Kosali Breed of Cattle: A Developmental Study of Histological Changes during Embryonic Development