<div>Background: The basic plant material consisted of 9 mungbean and 5 urdbean genotypes. 42 different crosses were made comprising 13 Vigna radiata x V. radiata, 4 V. radiata x V. mungo and 25 V. radiata x V. mungo crosses. Varying degree of success has been achieved in obtaining intraspecific and interspecific crosses having desired variability for yield and yield contributing traits along with MYMV resistance. 
 
Methods: The present investigation was done at Agricultural Research Farm, Banaras Hindu University, Varanasi, during Kharif, 2019 and Summer, 2020. 42 intra-specific and inter-specific cross combinations were initially examined for their crossability. Out of these, six promising crosses with higher crossability percent were further analyzed for hybrid pollen fertility, hybrid lethality and, evaluation of F1 and parents for different yield traits 
. 
Result: Crossability per cent found highest in intraspecific cross HUM 2 x IPM 02-3 (41.86%) in mungbean while, in urdbean cross NDU 1 x R3/12 (41.38%). In interspecific crosses, the maximum crossability per cent was found in SKAU M 365 x R3/28 (34.92%). Two mungbean genotypes (HUM 2 and HUM 26) and two urdbean genotypes (R3/12 and R3/28) can be further utilized for genetic improvement through inter-specific hybridization as there cross combinations are better performing on the basis of yield attributes along with MYMV resistance.</div>

​Comparative Study of Crossability Behavior in Intra-specific and Inter-specific Crosses of Vigna radiata and Vigna mungo

<div>Background: Information on gene action governing yield and yield component traits need to be studied for adoption of effective breeding procedure to wards development and isolation of high yielding promising lines from the cross with resistant to bacterial leaf blight, blast and tolerance to low soil phosphorous. The present investigation was taken up in this context to estimate the gene effects for yield and yield components of the cross, YH3 &times; AKDRMS 21-54, using five parameter model of generation mean analysis.&nbsp; 
 
Methods: The experimental material, P1, P2, F1, F2 and F3 generations, for the investigation was generated from Kharif 2019 onwards and evaluated at Agricultural College, Bapatla during Rabi 2021-22. Gene effects for grain yield and yield attributing traits were studied.&nbsp; 
 
Result: Mean effects of all traits studied were significant indicating the presence of sufficient variation for the traits. Significance of scaling tests revealed additive-dominant model as inadequate, indicating the presence of epistasis. Digenic non-allelic interaction model with five parameters, namely, m, d, h, i and l revealed that the epistatic interaction model was adequate to explain the gene action for the traits studied in the present investigation. Dominance x dominance gene interaction was pre-dominant for grain yield per plant and majority of yield component traits studied coupled with duplicate epistasis, indicating the importance of population improvement approaches, such as bi-parental mating and recurrent selection, followed by isolation of purelines in later generations of the cross for effective improvement of yield and majority of the yield component traits.&nbsp;</div>

Generation Mean Analysis for Yield and Yield Component Traits in Inter-specific Cross of Rice (Oryza sativa L.)

<div>Background: Pedigree method of breeding in predominantly self-pollinated crop like dolichos bean enable exploiting only fixable genetic effects as a result of rapid increase in homozygosis. One or a few cycles of inter-mating in F2 generation help create populations with high frequency of desired recombinants which otherwise cannot be realized in later generations. The objective of the present investigation was to assess the impact of inter se mating in F2 population on nine quantitative traits&rsquo; (QTs) mean and variability parameters. 
 
Methods: Randomly selected 20 pairs of a single cross-derived F2 plants with flowering synchrony were inter se mated. Progenies derived from 20 paired crosses (designated as BIP F3 progenies) and those (F3) derived from their 40 F2 parents were evaluated for 9 QTs. Statistics such as mean, absolute range (AR) and standardized range (SR), variance (&sigma;2), additive genetic variance (&sigma;2A) and narrow-sense heritability (NS-h2) were estimated. BIP F3 progenies were compared with those of F3 progenies for QTs mean, AR/SR, &sigma;2, &sigma;2A and NS-h2. Significance of differences between BIP F3 progenies and F3 progenies for mean and &sigma;2 were examined using two-sample t test and Levene&rsquo;s tests, respectively.&nbsp; 
 
Result: The random mating in F2 population was effective in increasing the means, variances, &sigma;2A and NS-h2 of racemes plant-1, fresh pods node-1, fresh pods plant-1 and fresh pod yield plant-1 in dolichos bean. Considering that random mating followed by selfing is a method of genetic improvement for future breeding rather than for immediate use, our results suggest long-term genetic gain and better prospects of deriving superior pure-lines with desired traits/combination of traits from inter-mated population in dolichos bean.</div>

<div>Effect of F2 Inter se Mating on Quantitative Traits Mean, Range, Variance and Heritability in Dolichos Bean (Lablab purpureus L. Sweet var. Lignosus)</div>

<div>Background: Combining ability is a measure that enables breeders to select better parents and outstanding hybrids. General combining ability and specific combining ability is used to determine best performing parents and vigorous hybrids respectively and provides an opportunity to understand the nature of gene action involved in inheritance of each character. 
 
Methods: A combining ability analysis was carried out with six mungbean varieties and their 15 F2 crosses derived from half diallel fashion. Data was recorded for 10 characters and were subjected to statistical analysis using method-II and model-1 of Griffing&rsquo;s approach. 
 
Result: There was importance of both additive and non-additive gene action in inheritance of all the characters as apprehended from significant values in mean sum of square due to GCA and SCA. Studies on components of variance due to GCA and SCA indicated there was preponderance of additive gene action for days to 50% flowering, days to maturity and pod length whereas non-additive gene action was expressed for other characters. Four parents viz., Kamdev, Pant Mung-8, MGG-385 and OBGG-52 were considered as best general combiners on the basis of significant GCA effects for a number of yield and its related traits. Based on SCA effects and per se performance, the crosses IPM-02-14/OBGG-58, OBGG-52/OBGG-58 and MGG-385/OBGG-52 displayed significant SCA in desirable direction along with high mean for many of the yield attributing characters. Highest significant SCA for seed yield was observed by the cross Kamdev/IPM-02-14. These selected parents and crosses can be used for further breeding programmes for crop improvement in mungbean.</div>

Combining Ability Studies for Seed Yield and its Quantitative Traits in F2 Crosses of Mungbean [Vigna radiata (Wilczek)]

<div>Background: Among several reasons for low productivity in mungbean, various biotic and abiotic factors play a major role. Although intensive research work has been done on genetic architecture of yield and yield attributes of mungbean but limited work was done on yield attributes along with water use efficiency (WUE) and heat stress tolerance related traits. The present study is aimed to identify the genetic potential of mungbean genotypes with high yield and drought and heat stress tolerance. 
 
Methods: The F2 seed was harvested from selfed F1 population during 2017-18 from three superior crosses viz., ML 267&times;LGG 528, MGG 390&times;LM 95 and LM 95&times;EC 362096. These crosses were selected based on their per se performance of yield, yield attributes and WUE related traits. Genetic analysis was carried out in F2 populations of three crosses for fourteen quantitative characters to identify the number of transgressive segregants in desirable direction. 
 
Result: A good number of transgressive segregants in desirable direction were observed for plant height, number of pods per cluster, harvest index, SLA, SLW and relative injury in the cross ML 267&times;LGG 528: for plant height, number of branches per plant, SCMR, SLA, SLW and relative injury in the cross MGG 390&times;LM 95 and for plant height, number of pods per cluster, number of pods per plant SCMR, SLA, SLW, relative injury and seed yield in the cross LM 95&times;EC 362096.</div>

Transgressive Segregants in F2 Populations of Three Superior Crosses viz., ML 267&times;LGG 528, MGG 390&times;LM 95, LM 95&times;EC 362096 in Mungbean

<div>Bruchid (Callosobruchus maculatus F.) is the main constraint in cowpea production. It can be controlled by chemical application but it is hazardous and not eco-friendly, hence attempt has been made to derive bruchid resistant lines by screening 100 stabilized lines from interspecific cross between cowpea and rice bean. In this experiment, screening of 100 F7 segregants of cross DC615 (susceptible) &times; Dharwad local (resistant) along with checks (DC15, DCS47-1, RBHP-38 and IC18563) was carried out against bruchid through artificial infestation at the Department of Genetics and plant Breeding, University of Agricultural Sciences, Dharwad. Out of 100 segregants screened, 7 were highly resistant, 52 were moderately resistant, 21 were least susceptible, 12 were moderately susceptible and 8 were highly susceptible. The segregant F7:78-1-1 registered least values for bruchid infestation, seed damage at 30, 45 and 75 days, loss of vigour and seed viability loss.</div>

<div>Bruchid Resistant Studies using Stabilized Derivatives (F7 Families) of Interspecific Cross Cowpea &times; Rice Bean</div>

Background: The study aimed to measure canine thyroid-stimulating hormone (cTSH) levels and their relationship with total triiodothyronine (TT3), total thyroxine (TT4) and free thyroxine (fT4) in healthy and hypothyroid dogs. 
&nbsp; 
Methods: The study involved 77 dogs, with 67 healthy and 10 hypothyroid. The serum concentration of cTSH was measured using a canine-specific TSH-ELISA kit, while TT3, TT4&nbsp;and fT4&nbsp;were measured using RIA kits. 
&nbsp; 
Result: The Mean serum concentration of cTSH, TT3, TT4&nbsp;and fT4&nbsp;in healthy dogs was 2.03&plusmn;0.01 &micro;IU/ml, 0.85&plusmn;0.04 nmol/l, 20.81&plusmn;0.79 nmol/l and 12.59&plusmn;0.35 pmol/l, respectively. Mean cTSH, TT3, TT4&nbsp;and fT4, concentration of hypothyroid dogs was found to be 2.16&plusmn;0.03 &micro;IU/ml, 0.93&plusmn;0.07 nmol/l, 9.01&plusmn;0.69 nmol/l and 5.87&plusmn;0.82 pmol/l, respectively. Interquartile range (i.e. 25th&nbsp;to 75th&nbsp;percentile) for thyroid hormone (cTSH, TT3, TT4&nbsp;and fT4) in healthy dogs (n = 67) were 1.96-2.11 &micro;IU/ml, 0.64-0.96 nmol/l, 16.73-25.00 nmol/l and 11.00-14.40 pmol/l, respectively and for hypothyroid dogs (n = 10) were 2.09-2.22 &micro;IU/ml, 0.73-1.12 nmol/l, 7.50-10.29 nmol/l and 4.35-7.45 pmol/l, respectively.&nbsp; A significant difference (P&lt;0.05) was recorded in healthy and hypothyroid dogs in cTSH, TT4&nbsp;and fT4. 
&nbsp;

Studies on Canine Thyroid-stimulating Hormone (cTSH), Total Triiodothyronine (TT3), Total Thyroxine (TT4) and Free Thyroxine (fT4) in Healthy and Hypothyroid Dogs

<div>This study aims to investigate the effects of glycosaminoglycan (GAG) from Urechis unicinctus on the P2Y12 receptor signaling pathway in rat platelets. The concentrations of cyclic adenosine monophosphate (cAMP), thromboxane B2 (TXB2) and glycoprotein IIb/IIIa (GPIIb/IIIa) in rat platelets were determined using enzyme-linked immunosorbent assay (ELISA) kits. The phosphorylation levels of protein kinase A (PKA) and vasodilator-stimulated phosphoprotein (VASP) in rat platelets were detected through Western blot. The expression of P2Y12 gene in rat platelets was analyzed via real-time fluorescence quantitative PCR and reverse-transcription PCR. It was observed that GAG significantly increased the cAMP content (p &lt; 0.05, p &lt; 0.01) and decreased the TXB2 and GPIIb/IIIa concentrations (p &lt; 0.05, p &lt; 0.01) in rat platelets. GAG significantly enhanced the phosphorylation levels of PKA and VASP in rat platelets (p &lt; 0.01) and had a synergistic effect with the P2Y12 receptor blocker. GAG significantly reduced the expression level of P2Y12 gene in rat platelets (p &lt; 0.01). We speculated that GAG from U. unicinctus inhibited platelet aggregation in rats through the P2Y12 receptor signaling pathway.</div>

<div>Effects of Glycosaminoglycan from Urechis Unicinctus on the P2Y12 Receptor Signaling Pathway in Rat Platelets</div>

<div>Background: Among enterobacteria E. coli and Klebsiella spp. are of great concern in health care settings, as these bacteria sometimes may contaminate the milk due to unhygienic practices and poor udder condition which have been associated with various illnesses. Therefore, this study aimed to detect the carbapenem resistant E. coli and Klebsiella spp. of bovine milk origin with regard to the risk of human transfer via the food chain in community. 
 
Methods: Total 240 samples were collected from Ayodhya and Sultanpur districts of Eastern Plain Zone of Uttar Pradesh (India). Confirmation of E. coli and Klebsiella spp. isolates was done by using species specific uidA and 16S rRNA gene, respectively. Then, carbapenemase positive E. coli and Klebsiella spp. were confirmend by DDST, MBL E-strip test and PCR analysis by targeting (bla-NDM, bla-OXA-48 and bla-KPC). Antibiogram of all carbapenemase positive isolates was performed against 20 antibiotics of 12 different classes. 
 
Result: In the present study, total 74(30.83%) isolates were identified including 55(22.92%) E. coli and 19(7.92%) Klebsiella spp. by PCR, out of which 12(16.21%) isolates were confirmed as carbapenemase producers comprising 7(12.72%) E. coli and 5(26.31%) Klebsiella spp by DDST and E-strip. All carbapenemase positive E. coli were found 100% sensitive to polymyxin-B and chloramphenicol, while all Klebsiella spp. were 100% sensitive to amikacin and polymyxin-B. Resistance against imipenem, meropenem, cefotaxime, cefpodoxime, ceftazidime, ceftriazone, aztreonam and ampicillin ranged between 80.0%-100%. All carbapenemase positive isolates were found multidrug resistant. Carbapenemase genes bla-NDM and bla-KPC were detected in E. coli while bla-OXA-48 and bla-KPC were detected in Klebsiella spp.</div>

​Detection of Carbapenemase Genes (bla-NDM, bla-KPC, bla-OXA-48) in Escherichia coli and Klebsiella species, Isolated from Milk Samples of Bovine in Eastern Plain Zone of Uttar Pradesh

<div>The present study was carried out to investigate the role of acute phase proteins [Serum amyloid A (SAA) and milk amyloid A (MAA)] for diagnosis of sub-clinical mastitis in cross-bred cows. The study was conducted on 40 apparently healthy cross-bred cows, in the mid-lactation period and were equally divided into four groups [Group-I (Bacteriologically negative milk samples, California mastitis test (CMT) negative), Group-II (Bacteriologically negative with SCC &pound;200,000 cells/ml milk, CMT positive), Group-III (Bacteriologically negative with SCC &sup3;200,000 &pound;500,000 cells/ml milk, CMT positive) and Group-IV (Bacteriologically positive with SCC &sup3;200,000 &pound;500,000 cells/ml milk, CMT positive)]. On bacteriological examination of 57 milk samples, 27(47.36%) were found positive and 30 (52.63%) were found negative. A highly significant (P&lt;0.01) differences in the mean values of SCC were observed between groups except group I and II and group III and IV. Highly significant (P&lt;0.01) differences in mean values of SAA were reported between all groups except group I and II. Highly significant (P&lt;0.01) differences in mean values of MAA were observed between all groups except group III and IV. Results of the correlation matrix revealed strong positive correlations (P&lt;0.01) of SCC with SAA and MAA; and between SAA and MAA. The proposed cutoff points for SCC, SAA and MAA were &gt;200,000 cells/ml, 74 &micro;g/ml and 10 &micro;g/ml, respectively for the diagnosis of sub-clinical mastitis with high sensitivity (90% to 100%). The area under curve of the SAA was larger than those of SCC and MAA, which suggests that the SAA test was more accurate than SCC and MAA for the diagnosis of sub-clinical mastitis.</div>

Comparative Study of Crossability Behavior in Intra-specific and Inter-specific Crosses of Vigna radiata and Vigna mungo

Generation Mean Analysis for Yield and Yield Component Traits in Inter-specific Cross of Rice (Oryza sativa L.)

Effect of F₂ Inter se Mating on Quantitative Traits Mean, Range, Variance and Heritability in Dolichos Bean (Lablab purpureus L. Sweet var. Lignosus)

Combining Ability Studies for Seed Yield and its Quantitative Traits in F₂ Crosses of Mungbean [Vigna radiata (Wilczek)]

Transgressive Segregants in F₂ Populations of Three Superior Crosses viz., ML 267×LGG 528, MGG 390×LM 95, LM 95×EC 362096 in Mungbean

Bruchid Resistant Studies using Stabilized Derivatives (F₇ Families) of Interspecific Cross Cowpea × Rice Bean

Studies on Canine Thyroid-stimulating Hormone (cTSH), Total Triiodothyronine (TT₃), Total Thyroxine (TT₄) and Free Thyroxine (fT₄) in Healthy and Hypothyroid Dogs

Effects of Glycosaminoglycan from Urechis Unicinctus on the P₂Y₁₂ Receptor Signaling Pathway in Rat Platelets

Detection of Carbapenemase Genes (bla_-NDM, bla_-KPC, bla_-OXA-48) in Escherichia coli and Klebsiella species, Isolated from Milk Samples of Bovine in Eastern Plain Zone of Uttar Pradesh

Acute Phase Protein as Biomarker for Diagnosis of Sub-Clinical Mastitis in Cross-Bred Cows