Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Indian Journal of Animal Research, volume 54 issue 1 (january 2020) : 24-30

Effects of Glycosaminoglycan from Urechis Unicinctus on the P2Y12 Receptor Signaling Pathway in Rat Platelets

Chunying Yuan, Fei Miao, Jinghong Li, Qingman Cui
1Tianjin Marine Environmental Protection and Restoration Technology Engineering Center; Tianjin Key Laboratory of Marine Resource and Chemistry; College of Marine & Environment, Tianjin University of Science and Technology, Tianjin, China.
Cite article:- Yuan Chunying, Miao Fei, Li Jinghong, Cui Qingman (2019). Effects of Glycosaminoglycan from Urechis Unicinctus on the P2Y12 Receptor Signaling Pathway in Rat Platelets. Indian Journal of Animal Research. 54(1): 24-30. doi: 10.18805/ijar.B-1149.
This study aims to investigate the effects of glycosaminoglycan (GAG) from Urechis unicinctus on the P2Y12 receptor signaling pathway in rat platelets. The concentrations of cyclic adenosine monophosphate (cAMP), thromboxane B2 (TXB2) and glycoprotein IIb/IIIa (GPIIb/IIIa) in rat platelets were determined using enzyme-linked immunosorbent assay (ELISA) kits. The phosphorylation levels of protein kinase A (PKA) and vasodilator-stimulated phosphoprotein (VASP) in rat platelets were detected through Western blot. The expression of P2Y12 gene in rat platelets was analyzed via real-time fluorescence quantitative PCR and reverse-transcription PCR. It was observed that GAG significantly increased the cAMP content (p < 0.05, p < 0.01) and decreased the TXB2 and GPIIb/IIIa concentrations (p < 0.05, p < 0.01) in rat platelets. GAG significantly enhanced the phosphorylation levels of PKA and VASP in rat platelets (p < 0.01) and had a synergistic effect with the P2Y12 receptor blocker. GAG significantly reduced the expression level of P2Y12 gene in rat platelets (p < 0.01). We speculated that GAG from U. unicinctus inhibited platelet aggregation in rats through the P2Y12 receptor signaling pathway.
  1. Campo, G.M., Ascola, A.D., Avenoso, A., Campo, S., Ferlazzo, A.M., Micali, C., Zanghi, L., Calatroni, A. (2004). Glycosaminoglycans reduce oxidative damage induced by copper (Cu2+), iron (Fe2+) and hydrogen peroxide (H2O) in human fibrob last cultures. Glycoconj J. 20: 133-141.
  2. Collaboration, A.T. (2002). Collaborative meta-analysis of randomized trials of antiplatelet therapy for prevention of death, myocardial infarction and stroke in high risk patients. Br Med J. 324: 71-86.
  3. Cui, Q.M., Liu, P., Wang, Y., Yuan, C.Y. (2015). Study on anticoagulant mechanism of Glycosaminoglycan from Urechis unicinctus. Sci Tech Food Ind. 36: 337-340.
  4. Gachet, C. (2006). Regulation of platelet unctions by P2 receptors. Annu Rev Pharmacol Toxicol. 46: 277-300.
  5. Guidetti, G.F., Lova, P., Bernardi, B., Campus, F., Baldanzi, G. (2008). The Gi-coupled P2Y12 receptor regulates diacylglycerol-mediated signaling in human platelets. J Biol Chem. 283: 287-295. 
  6. Han, C.F. (2016). Development of antiplatelet agents for thrombotic diseases. Chin J New Drug. 25: 1363-1369.
  7. Jin, H.X. (2006). Molecular modelling study on structure and function of protein kinase A and its inhibitor. Beijing: Zhejiang Uni. 2-8.
  8. Li, G.J., Cui, Q.M., Yuan, C.Y. (2012). Study on chemical composition and biological activities of glycosaminoglycan from Patinopecten yessoensis waste. Sci Tech Food Ind. 33: 132-137.
  9. Li, M. (2013). Advance in the mechanism of anti-platelet aggregationdrugs. Cap Med, 9:15-18.
  10. Mangin, P., Yuan, Y., Goncalves, I., Eckly, A., Freund, M., Cazenave, J.P., Gachet, C., Jackson, S.P., Lanza, F. (2003). Signaling role for phospholipase Cã2 in platekt glycoprotein Ibácalcium flux and cytoskeletal reorganization. Involvement of a pathway distinct from FcR gamma chain and Fc gamma RIIA. J Biol Chem. 278: 32880-32891.
  11. Miao, F., Zhao, Z.Y., Cui, Q.M., Yuan, C.Y. (2018). Anti-platelet aggregation effect of glycosaminoglycan from Urechis unicinctus in rats. Nat Prod Res Dev. 30: 2187-2192.
  12. Shrestha, S.S., Parmar, M., Kennedy, C., Bushell, T.J. (2010). Two-pore potassium ion channels are inhibited by both Gq/11 and Gi-    coupled P2Y receptors. Mol Cell Neurosci. 43: 363-369.
  13. Yuan, C.Y., Cui, Q.M., Xu, J.L., Li, G.J. (2013). Biological activities and spectral analysis of clycosaminoglycan from Bullacta exarata. Guangdong Agr Sci. 40:173-175.
  14. Yuan, C.Y., Liu, P., Han, X., Cui, Q.M. (2014). The study on composition structure and functional activity of glycosaminoglycan from Urechis unicinctus. Food Ind. 35: 181-183.
  15. Zhai, T., Hua, S.Y. (2017). Research progress of platelet ADP receptor and its antagonist. Tianjin J Tradit Chin Med. 34: 280-284.
  16. Zhang, Y.Z., Chen, B.L. (2013). Research progress on the P2Y12 receptor antagonists. Chin J Clin Pharmacol Ther. 18: 824-830.
  17. Zhuang, X.Q., Huo, J.L., Yang, T.Y., Kong, Y. (2010). Research progress of platelet ADP receptor and its antagonist. Pharm Biotechno. 17: 278-282.

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