<div>Background: Traumatic pericarditis is one of the major problems encountered in cattle resulting in greater economic loss to the farming community and causes mortality. Diagnosis of heart diseases in animals is a challenging job for Veterinarians, bovines ingest foreign bodies (nails, thorns, glass pieces, metal wires, etc.) along with the feed and fodder which intern entangled in reticulum leading to traumatic pericarditis. The affected cattle shows drop in milk yield, sometimes death of affected animals especially pregnant or milking. The main aim of present study enlightens on diagnosis and surgical management of fibrinous pericarditis. 
 
Methods: The clinical cases presented to Veterinary College Hospital, Hassan, suspected for pericarditis were selected for the present study, these animals were subjected for clinical, haemato-biochemical, ECG, radiological and ultrasonographic examination. In the total of 38 animals, six cattle were diagnosed as fibrinous pericarditis were subjected for pericardiotomy. Post operatively parenteral antibiotic and supportive therapy was given for 5-7 days.&nbsp; 
 
Result: 5th rib resection followed by pericardiotomy gave favourable result in animals suffering with fibrinous pericarditis. So early detection timely employment of suitable surgical technique can prolong the life of animal and can save the life of the young one.</div>

Fibrinous Pericarditis and its Surgical Management in Cattle

<div>Background: Traumatic injury caused by swallowed&nbsp; sharp foreign object is one of the common conditions in dairy animal resulting into development of traumatic pericarditis (TP) and traumatic reticuloperitonitis (TRP). Under field conditions both conditions mimic the same clinical signs making it difficult to differentiate as well as render to choose ideal therapeutic management. The present study was aimed to evaluate clinical, hematobiochemical and ultrasonographic changes in cattle to clinically differentiate between TP and TRP cases. 
 
Methods: From the period of January 2020 to December 2020, total twelve Holstein Friesian cattle were investigated for TP and TRP. In the present study, six animals each suffering from TP and TRP were included along with six normal healthy animals as control. Different clinical signs, haemato-biochemical parameters and ultrasonographical findings were recorded in each group and comparative analysis was done. 
 
Result: Brisket edema, bilateral jugular vein engorgement and arched back conditions were most reported clinical signs in both the groups. Significant changes were recorded in the values of red blood cells, lymphocyte, blood urea nitrogen, creatinine and SGOT between both the groups. Significant drop in hemoglobin level was observed in TP affected group. No significant changes were observed in white blood cells, packed cell volume, monocyte counts and eosinophil counts. Significant increase in fibrinogen concentration recorded in both the groups. In ultrasonography, accumulation of anechoic fluid around heart in TP and reticular wall thickening in TRP was most consistent findings.&nbsp;</div>

A Clinical Study on Traumatic Pericarditis and Traumatic Reticuloperitonitis in Twelve Holstein Friesian Cattle

<div style="text-align:justify">
Background: To evaluate use of homologous platelet rich plasma (PRP) drop and leucocyte-platelet rich fibrin (L-PRF) clot/membrane as regenerative therapy for healing of corneal ulcer in dogs and its subsequent comparison with conventional therapy. 
 
Methods: In this randomized study, 30 dogs were divided into three groups of 10 animals each. It was ensured each group had grade I, II and III ulcers. Group I received medicinal treatment with antimicrobial and non-steroidal anti-inflammatory eye drop. In Group II PRP drops and in Group III L-PRF membrane was used to evaluate corneal ulcer healing. Homologous platelet concentrates were prepared in laboratory and used for assessment of healing on various grades of corneal ulcer on basis on reduction in size and depth of ulcer, presence of stromal infiltration, scleral involvement, corneal opacity and vascularization. 
 
Result: Post treatment, the size of corneal ulcer started to reduce gradually from day 7 onwards. Significant (p&le;0.05) reduction was observed from day 10 in group I after instillation of drops Moxifloxacin and Flurbiprofen and in group II after instillation of PRP. It was measured as 5.35 &plusmn; 0.63 and 5.20 &plusmn; 0.98 mm at day 0, which reduced significantly (p&le;0.05) to 3.90 &plusmn; 0.50 and 3.10 &plusmn; 0.98 mm at day 10 in group I and group II respectively. In group III there was significant reduction in size of ulcer from day 0 to day 7 measuring 7.80 &plusmn; 1.05 and 0.20 &plusmn; 0.11mm respectively. Thereafter, from day 10 onwards complete sealing of ulcer was observed in all the cases. Post treatment there was no significant reduction in corneal opacity from day 0 to day 30 in group I dogs and corneal opacity persisted specially in grade II and grade III ulcers. There was significant reduction (p&le;0.05) in opacity from 2.90 &plusmn; 0.35 to 2.10 &plusmn; 0.38 and 3.00 &plusmn; 0.37 to 2.40 &plusmn; 0.34 in group II and group III dogs from day 0 to day 10 respectively. In group I, II and III there was significant (p&le;0.05) reduction in vascularization from 1.50&plusmn;0.30 to 1.20&plusmn;0.13, 1.30&plusmn;0.37 to 0.60&plusmn;0.22 and 1.40&plusmn;0.31 to 0.40&plusmn;0.31 from day 0 to day 15 respectively. There was a significant reduction in vascularization on day 15 in group II and group III compared to group I animals.
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​Evaluation of Homologous Platelet Rich Plasma (PRP) Drop and Leucocyte Platelet Rich Fibrin (L-PRF) Membrane for Corneal Ulcer Healing in Dogs

<div style="text-align:justify">Infectious laryngotracheitis (ILT), caused by Gallid herpesvirus1, is one among the respiratory diseases of poultry gradually spreading worldwide, including Indian subcontinent. Present study was carried out to identify the pathogen from the suspected cases of the disease. The tracheal tissue samples (pooled) were collected from the birds suspected to have died of ILT from 26 commercial poultry farms located in and around Namakkal district of Tamil Nadu state of India. On post-mortem examination, haemorrhagic caseous exudate and fibrinous pseudo-membrane adhered to tracheal mucosa were noticed. A total of 22 farms were found positive through PCRs targeted against ICP4 gene and the thymidine kinase (TK) gene followed by confirmation through sequencing. Histopathology examination revealed decilliation, hyperplasia, degeneration and/or loss of tracheal epithelium with severe submucosal edema. There was infiltration with numerous lymphocytes, macrophages and plasma cells in milder infections, whereas presence of fibrinous exudates admixed with numerous erythrocytes and inflammatory cells like heterophils and lymphocytes were seen indicating the severe acute form of the disease. A fibrinous pseudomembrane was seen firmly attached to the inflamed and necrotic mucosa in subacute cases. Further, virus was isolated from randomly selected 5 PCR positive tracheal tissue samples in embryonated chicken eggs through chorioallantoic membrane (CAM) route. The typical pock lesions were observed on CAM along with engorged blood vessels and thickening of the membrane. Present study has reported the disease ILT among poultry flocks in the Namakkal district of Tamil Nadu and raises the concern for thorough investigation of the nature of prevailing pathogen in the region.</div>

<div style="text-align: justify;">Infectious Laryngotracheitis in Layer Birds from Tamil Nadu, India</div>

Background: Mycoplasmal pneumonia causes severe economic loss in goat farms due to loss of production performance and mortality. Mycoplasma ovipneumoniae is an important pathogen causing mycoplasmal pneumonia in small ruminants. The present research work was carried out to study the pathomorphological alterations associated with M. ovipneumoniae infection in an organized goat farm. 
 
Methods: The research work was carried out in an organized goat farm having history of respiratory illness with mortality and suspected for mycoplasmal pneumonia. Samples from ailing animals and dead animals were collected for investigation. Tissue samples from lungs, trachea, mediastinal lymph nodes and swabs from lungs, trachea and pleural fluid were collected during postmortem examination and subjected to laboratory examination. 
 
Result: The samples were confirmed to be positive for M. ovipneumoniae infection by PCR using specific primers. The clinical signs of M. ovipneumoniae infection observed were anorexia, mucopurulent nasal discharge, coughing, increased body temperature, respiratory distress and death in severely affected animals. In the present study, 10 animals exhibited clinical signs and 3 animals died out of 10 animals. Necropsy lesions observed in lungs were fibrinous adhesions, severe consolidation of left lung and blackish discoloration of right lung. Histopathologically, lesions in lungs were characterized by widespread congestion, presence of inflammatory cells, widened interlobular septa with fibrin and inflammatory cells. Congestion of bronchioles and presence of pinkish fibrinous exudate within the bronchiolar lumen were observed. From the present study it is concluded that M. ovipneumoniae infection causes severe pathology of respiratory organs and mortality in goats under stressful conditions like long transport.

Pathomorphology of Mycoplasmal Pneumonia Associated with Mycoplasma ovipneumoniae Infection in an Organized Goat Farm

<div>Background: Pathomicrobial and molecular investigations of respiratory system diseases was undertaken on twelve adult buffalo carcasses received for necropsy examination at post mortem facility of Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana during the period from August, 2018 to February, 2019.&nbsp; 
 
Methods: After collection of the samples, laboratory work was undertaken in the laboratories of Department of Veterinary Pathology, Department of Animal Biotechnology and Central Laboratory of the College, LUVAS, Hisar, Haryana (India) in the year 2018-2019 regarding the examination of the clinical history, pathological, microbiological and molecular investigations. 
 
Result: The gross pathological changes observed in lungs were variable degree of vascular changes, mild to severe consolidation, fibrin deposition along with adhesions to the thoracic wall. Histopathological findings revealed abnormalities of inflation such as pulmonary emphysema, atelectasis, pulmonary congestion and haemorrhages which was associated with different types of pneumonia viz. fibrinous bronchopneumonia, suppurative giant cell pneumonia, interstitial pneumonia and serous pneumonia. Lung, heart blood and tracheal swab samples collected from the buffalo carcasses revealed eighteen bacterial isolates which were identified by Vitek 2 system. These include E. coli (11 isolates), Salmonella enteric enterica (2 isolates), Acinetobacter ursingii (1 isolate), Staphylococcus haemolyticus (1 isolate), Staphylococcus sciuri (1 isolate), Staphylococcus warneri (1 isolate) and Staphylococcus hominis (1 isolate) mostly belonging to opportunistic pathogen category. E. coli serotypes confirmed from these cases were O83, O149 and O8. The results of in vitro drug sensitivity testing revealed that most of bacterial strains were found sensitive to cefoperazone/sulbactum and co-trimoxazole. Molecular studies confirmed Bovine Herpes Virus-1 (BHV-1) infection in one case with Fibrinous bronchopneumonia through real-time quantitative PCR indicating the prevalence of the infection in the state.</div>

Pathomicrobial and Molecular Investigations of Respiratory System Diseases Affecting Buffaloes

<div style="text-align: justify;">Ultrasonography helps in early diagnosis of large animal digestive disorders especially in cattle with empty rectum and also to initiate early therapeutic and surgical management. The study was undertaken to elicit GI disturbances in cattle at TVCC, VCRI, Orathanadu during 2015-16. Animals with history of absence of feces and or empty rectum were selected for this study. In total twenty nine cattle were found to be affected with various GI disorders. The incidence of GI disturbances was more (65.52 %) in cross bred Jersey cow. The common clinical signs were absence of feces (65.52 %), anorexia (58.62 %), scant/tarry/mucous coated foul smelling feces (34.48 %), arched back (24.14 %) and abdominal distention (24.14 %). All selected 29 animals were subjected to abdominal ultrasonography which revealed peritonitis (31.03 %), paralytic ileus with peritonitis and pericarditis in each 10.34 %, uroabdomen, intussusceptions, caecal dilatation, abomasal dilatation and peritonitis with intussusceptions in each 6.90 % and diaphragmatic hernia, reticular abscess, omasal impaction and peritonitis with pericarditis in each 3.45 %. Based on this study, clinical and ultrasonographic examinations were found to be a useful tool for the diagnosis of GI disorders of cattle with empty rectum.&nbsp;</div>

Clinical and Ultrasonographic examinations in cattle with empty rectum

<div>Background: Respiratory diseases are very common in goats and cause severe economic loss to the rural poor farmers. Proper identification of the actual pathogen(s) responsible for the respiratory infections is critical for timely and proper management of those diseases. The present communication describes the spontaneous occurrence of concurrent Peste des petits ruminants (PPR) and contagious caprine pleuro-pneumonia (CCPP) in a goat flock. 
 
Methods: An outbreak of respiratory disease in a goat flock consisting of 45 Tellicherry cross bred animals in varying age groups was investigated during the month of February 2019 in Namakkal district of Tamil Nadu. Information of disease outbreak and flock details were collected. Samples from live (nasal, ocular and rectal swabs) and dead (lung, spleen and mesenteric lymph nodes) were collected for detection of Peste des petits ruminants virus (PPRV) and Mycoplasma capricolum subsp.capripneumoniae (MCCP) antigen by polymerase chain reaction. Tissues from heart, lung, liver, kidney, intestine, spleen and lymph node were collected in 10% neutral buffered formalin for histopathological examination. 
 
Result: Out of 45 goats at risk, 23 showed clinical signs and 17 died of disease conditions with morbidity, mortality and case fatality rate of 51.11%, 37.77% and 73.91% respectively. PPR was confirmed by RT-PCR with M gene specific primers (191bp) and CCPP was confirmed by PCR with 16S rRNA gene fragment (316 bp). Affected animals showed clinical signs of pyrexia, anorexia, marked depression, mild erosions in the oral mucosa, nasal and ocular discharge, coughing and diarrhoea. Necropsy examination revealed congested oral mucosa, extensive pleuritis with large fibrin clots on lung surface and greyish pink consolidation of cranial lobes and anterior parts of diaphragmatic lobes. On section, consolidated areas were granular in appearance. Histopathology of lung revealed thickening of pleura, interlobular septa and alveolar wall by fibrin deposition and infiltration of inflammatory cells. &nbsp;Pulmonary congestion, infiltration of neutrophils and mononuclear cells, syncytial cell formation with eosinophilic intracytoplasmic and intranuclear inclusions were also noticed. Visceral organs showed degenerative and necrotic changes.</div>

<div>Pathomorphology of Concurrent Peste des Petits Ruminants (PPR) and Contagious Caprine Pleuropneumonia (CCPP) in a Cross Breed Goat Flock</div>

Background:&nbsp;Mannheimia is a Gram-negative, facultative anaerobe, non-spore forming and non-motile bacteria belongs to the family Pasteurellaceae. Caprine bacterial pneumonia is most often caused by Mannheimia haemolytica (previously known as Pasteurella haemolytica) and Pasteurella multocida which are more frequently associated with the outbreak of acute pneumonia and death of goats, particularly young once. Mannhemia is an opportunistic pathogen that particularly inhabits the nasopharynx and tonsils of goat and sheep. The present study was aimed to study Mannheimiosis in Guwahati, Assam in order to diagnose and control the disease among goats. 
 
Methods: The present investigation was conducted during the year 2021-2022 in Guwahati, Assam. A total of 51 pneumonic lung samples were collected from nearby slaughter houses and during necropsy at Department of Pathology, C.V.Sc., A.A.U., Khanapara, Guwahati-22. All the samples were subjected to primary isolation in blood agar and MacConkey&#39;s agar and further confirmation was made using PCR. 
 
Result: Out of total 51 pneumonic lung samples examined seven (13.72%) cases were found positive for Mannheimia haemolytica infection where 5 cases were found to be associated with other respiratory pathogens.&nbsp;Visible gross lesions observed during necropsy were broncho-interstitial pneumonia, suppurative pneumonia and fibrinous pneumonia with hemorrhagic tracheitis. Histopathological examination revealed presence of characteristic oat cells, intravascular thrombosis, fibrin balls, oedema, necrosis and polymorphonuclear cell infiltration in the lungs. Bacteriological culture for isolation and identification of Mannheimia haemolytica showed &beta; haemolytic zone on MLA plates. Confirmatory diagnosis was made using PCR targeting 16s rRNA gene with amplicon size 1500 bp and Lkt gene with amplicon size 206 bp. The phylogenetic analysis of 16s rRNA gene from positive sample showed percent identity of above 97% with other strains of Mannheimia haemolytica present in NCBI website. The variability in the lesions observed in the present study it can be concluded that a mixed infection with reduced resistance of the animals contributed to the development of pneumonia rather than Mannheimia haemolytica alone and PCR can be used as reliable technique for confirmatory diagnosis of respiratory mannheimiosis.

Pathomorphological and Molecular Studies of Respiratory Mannheimiosis in Goats

Contagious bovine pleuropneumonia (CBPP) is a bacterial disease, the causative agent of which is Mycoplasma mycoides subsp. Mycoides. The CBPP disease is one of important disease to be given attention because it is causing heavy mortality in bovines all over the world with African continent being hardly hit due to vagaries of bacteria. Air droplets is primary source of infection. Colonization in lower respiratory tract, Mmm invades arteries cause vasculitis. Many pathways of Mmm organism to cause destruction in host animal, presence of Gts ABC is one such mechanism. In CBPP we see pulmonary sequestra in chronic cases, severe fibrinous bronchopneumonia and pleural effusion during the acute to subacute phases. For confirmatory diagnosis, laboratory investigation and use of molecular techniques is recommended. Isolation of causative agent is also optimal method for diagnosis. The use of danofloxacin, tetracycline, flufenicol etc have been documented with varying degree of efficiency. Control over movement of bovine species, adequate space (where droplet spread is prevented) for each animal and vaccination are recommendatory steps. Though India has been declared CBPP free in 2007 we still need cautious approach and continue surveillance because north east of India seems very vulnerable to CBPP. Also, the fact that animal husbandry and agriculture drive economy of some of our most populous states it is all the more important that we give due consideration and focus on CBPP.

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