Legume Research

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Legume Research, volume 45 issue 10 (october 2022) : 1241-1246

Alleviation of Calcium Toxicity in Arabidopsis thaliana by Overexpressing GmHsp90s from Soybean

Xu Jinyan, Guo Na, Zhao Jinming, Xing Han, Xue Chenchen
1nstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

  • Submitted07-03-2022|

  • Accepted20-06-2022|

  • First Online 22-08-2022|

  • doi 10.18805/LRF-686

Cite article:- Jinyan Xu, Na Guo, Jinming Zhao, Han Xing, Chenchen Xue (2022). Alleviation of Calcium Toxicity in Arabidopsis thaliana by Overexpressing GmHsp90s from Soybean. Legume Research. 45(10): 1241-1246. doi: 10.18805/LRF-686.

ABSTRACT

Background: In agriculture, supplemental calcium was applied to alleviate plant growth and development inhibition causing by various stresses. However, calcium overload is toxic to plants, which may prevent the germination of seeds and reduce plant growth rates. Hsp90 is an important molecular chaperone distributing in all living organisms and a series of studies have shown that Hsp90 and Ca2+ have closely relationship. To better understanding the relationship between GmHsp90s and calcium stress, we conducted a series of experiments and reported in this research article.
Methods: The study was performed by three techniques: 1) Quantitative RT-PCR with five GmHsp90 genes viz., GmHsp90A2, GmHsp90A4, GmHsp90B1, GmHsp90C1.1 and GmHsp90C2.1, 2) MDA, O2- and chlorophyll content assay of transgenic plants after calcium stress and 3) Phenotype analysis of transgenic plants in pod setting period after three days treatment of water or 80 mM CaCl2.
Result: Quantitative RT-PCR with the five genes showed that they were all CaCl2 inducible. MDA, O2- and chlorophyll content assay showed that GmHsp90A2 and GmHsp90A4 transgenic lines significantly relieved the damage caused by CaCl2 and oxidative stress. The secondary stress damage, including the effect on plant height and pod setting rate, was also reduced in transgenic lines, especially GmHsp90B1 and GmHsp90C1.1 transgenic lines. Collectively, this study reveals the response of GmHsp90s to calcium and their potential function in coping with calcium stress.

REFERENCES


  1. Alharby, H.F., Alsamadany, H., Hakeem, K.R., Alzahrani, Y.M. (2020). Impact of Drought and Calcium Sulfate on Antioxidants, S-Assimilation, Ecophysiology and Growth of Tomato (Lycopersicon esculentum). International Journal of Agriculture and Biology. 23(1): 215-226.

  2. An, P., Li, X., Zheng, Y., Eneji, A.E., Inanaga, S. (2014). Calcium effects on root cell wall composition and ion contents in two soybean cultivars under salinity stress. Canadian Journal of Plant Science. 94(4): 733-740.

  3. Biswas, C., Ostrovsky, O., Makarewich, C.A., Wanderling, S., Gidalevitz, T., Argon, Y. (2007). The peptide-binding activity of GRP94 is regulated by calcium. Biochemical Journal. 405: 233-241.

  4. Charpentier, M. (2018). Calcium signals in the plant nucleus: Origin and function. Journal of Experimental Botany. 69(17): 4165-4173.

  5. Chong, L.P., Wang, Y., Gad, N. anderson, N., Shah, B., Zhao, R. (2015). A highly charged region in the middle domain of plant endoplasmic reticulum (ER)-localized heat-shock protein 90 is required for resistance to tunicamycin or high calcium-induced ER stresses. Journal of Experimental Botany. 66(1): 113-124.

  6. Clement, M., Leonhardt, N., Droillard, M.J., Reiter, I., Montillet, J.L., Genty, B., Lauriere, C., Nussaume, L., Noel, L.D. (2011). The cytosolic/nuclear HSC70 and HSP90 molecular chaperones are important for stomatal closure and modulate abscise acid-dependent physiological responses in Arabidopsis. Plant Physiology. 156 (3): 1481-1492.

  7. Eletto, D., Dersh, D., Argon, Y. (2010). GRP94 in ER quality control and stress responses. Seminars in Cell and Developmental Biology. 21(5): 479-485.

  8. Elstner, E.F. and Heupel, A. (1976). Inhibition of nitrite formation from hydroxylammoniumchloride: A simple assay for superoxide dismutase. Analytical Biochemistry. 70: 616-620.

  9. Erbil, E. (2021). The effects of gibberellic acid (GA3) applications on the physiological features of groundnut (Arachis hypogaea L.) under different salt (NaCI) stress conditions. Legume Research. 44(10): 1159-1163.

  10. Hodges, D.M., DeLong, J.M., Forney, C.F., Prange, R.K. (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta. 207: 604-611.

  11. Kudze, T., Mendez-Dorantes, C., Jalloh, C.S., McClellan, A.J. (2018). Evidence for interaction between Hsp90 and the ER membrane complex. Cell Stress Chaperones. 23(5): 1101-1115.

  12. Mittler, R. and Blumwald, E. (2015). The roles of ROS and ABA in systemic acquired acclimation. The Plant Cell. 27 (1): 64-70.

  13. Leng, X. H., Xue, L., Wang, J., Li, S., Yang, Z.L., Ren, H.D., Yao, X.H., Wu, Z.Y., Li, J.Y. (2020). Physiological Responses of Handeliodendron bodinieri (Levl.) Rehd. to Exogenous Calcium Supply under Drought Stress. Forests. 11: 69.

  14. Nomura, H. and Shiina, T. (2014). Calcium signaling in plant endosymbiotic organelles: Mechanism and role in physiology. Molecular Plant. 7(7): 1094-1104.

  15. Park, H.K., Lee, J.E., Lim, J., Kang, B.H. (2014). Mitochondrial Hsp90s suppress calcium-mediated stress signals propagating from mitochondria to the ER in cancer cells. Molecular Cancer. 13: 148.

  16. Peng, T.I. and Jou, M.J. (2010). Oxidative stress caused by mitochondrial calcium overload. Annals of the New York Academy of Sciences. 1201(1): 183-188.

  17. Porra, R., Thompson, W., Kriedemann, P. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (BBA)-Bioenergetics. 975: 384-394.

  18. Ritu, J. (2006). Role of calcium in the physiology of plants - A review. Agricultural Reviews. 27(4): 235-246.

  19. Saito, S. and Uozumi, N. (2020). Calcium-Regulated Phosphorylation Systems Controlling Uptake and Balance of Plant Nutrients. Frontiers in Plant Science. 11: 44.

  20. Sanjeev, K., Nikki, B., Saravaiya, S.N. (2018). Vegetable grafting: A surgical approach to combat biotic and abiotic stress - A review. Agricultural Reviews. 39(1): 1-11.

  21. Schmedes, A. and Hølmer, G. (1989). A new thiobarbituric acid (TBA) method for determining free malondialdehyde (MDA) and hydroperoxides selectively as a measure of lipid peroxidation. Journal of the American Oil Chemists’ Society. 66: 813-817.

  22. Thor, K. (2019). Calcium-Nutrient and Messenger. Frontiers in Plant Science. 10: 440.

  23. White, P.J. and Broadley, M.R. (2003). Calcium in Plants. Annals of Botany. 92: 487-511.

  24. Xu, Z.S., Li, Z.Y., Chen, Y., Chen, M., Li, L.C., Ma, Y.Z. (2012). Heat shock protein 90 in plants: molecular mechanisms and roles in stress responses. International Journal of Molecular Sciences. 13(12): 15706-15723.

  25. Xu, J., Xue, C., Xue, D., Zhao, J., Gai, J., Guo, N., Xing, H. (2013). Overexpression of GmHsp90s, a heat shock protein 90 (Hsp90) gene family cloning from soybean, decrease damage of abiotic stresses in Arabidopsis thaliana. PLoS One. 8(7): e69810.

  26. Zhang, H., Li, Z.H., Zhang, M.Q., Katz, M.S., Zhang, B.X. (2008). Heat shock protein 90â1 is essential for polyunsaturated fatty acid-induced mitochondrial Ca2+ efflux. The Journal of Biological Chemistry. 283 (12): 7580-7589.
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