Field-based Screening and Haplotyping of J Locus for Long Juvenile Trait in Tropical Soybean Genotypes

DOI: 10.18805/LR-603    | Article Id: LR-603 | Page : 515-521
Citation :- Field-based Screening and Haplotyping of J Locus for Long Juvenile Trait in Tropical Soybean Genotypes.Legume Research.2021.(44):515-521
Enoch Sapey, Yudong Fang, Dalia Mohamedkheir Khojely, Wenwen Song, Bingjun Jiang, Shan Yuan, Shi Sun, Tianfu Han hantianfu@caas.cn
Address : MARA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Submitted Date : 8-12-2020
Accepted Date : 2-03-2021


Background: The long juvenile trait influences flowering time of soybean under tropical conditions. The trait ensures sufficient vegetative growth prior to flowering. The present study aimed at identifying tropical soybean genotypes with the long juvenile trait and harboring the loss-of-function alleles for the J gene and verifying the effect of loss-of-function alleles on the long juvenile trait. 
Methods: A total of 159 soybean genotypes were evaluated on the field for two years in tropical city of Sanya, Hainan Islands, China for days to beginning bloom (VE-R1), days to physiological maturity (VE-R7), nodes/plant, pods/plant and plant height at maturity. The full length of the J gene was cloned in the 159 soybean genotypes. The sequence data was subjected to haplotype analysis to determine the genotypes with the functional and loss of function alleles. 
Result: Significant differences (p<0.05) were observed among genotypes for all phenotypic traits. 53 genotypes were identified with delayed flowering based on the phenotypic data. Sequence comparison of the 159 genotypes identified 10 polymorphisms comprising 7 SNPs and three deletions in the coding sequences, the three deletions resulted in the loss of function of the J gene. Six genotypes with delayed flowering had the loss-of-function alleles of the J gene.


Delayed flowering Haplotypes Long juvenility Soybean


  1. Bonato, E.R. and Vello, N.A.J.G. (1999). E6, a dominant gene conditioning early flowering and maturity in soybeans. Journal of Genetics and Molecular Biology. 22: 229-232.
  2. Carpentieri-Pipolo, V., de Almeida, L.A., Kiihl, R.A.D. and Rosolem, C.A. (2000). Inheritance of long juvenile period under short day conditions for the BR80-6778 soybean [Glycine max (L.) Merrill] line. Euphytica. 112: 203-209.
  3. Cober, E.R. and Morrison, M.J. (2010). Regulation of seed yield and agronomic characters by photoperiod sensitivity and growth habit genes in soybean. Theoretical and Applied Genetics. 120: 1005-1012.
  4. Destro, D., Carpentieri-Pípolo, V., Kiihl, R.d.S. and de Almeida, L.A. (2001). Photoperiodism and genetic control of the long juvenile period in soybean: A review. Crop Breeding and Applied Biotechnology. 1: 72-92.
  5. Fang, X., Han, Y., Liu, M., Jiang, J., Li, X., Lian, Q., Xie, X., Huang, Y., Ma, Q., Nian, H., et al. (2020). Modulation of evening complex activity enables north-to-south adaptation of soybean. Sci China Life Sci. 64: 179-195.
  6. Fehr, W., Caviness, C., Burmood, D. and Pennington, J. (1971). Stage of development descriptions for soybeans, Glycine max (L.) Merrill. Crop Science. 11: 929-931.
  7. Hartwig, E.E. and Kiihl, R.A. (1979). Identification and utilization of a delayed flowering character in soybeans for short-day conditions. Field Crops Research. 2: 145-151.
  8. Hymowitz, T. (1970). On the domestication of the soybean. Economic Botany. 24: 408-421.
  9. Lu, S.J., Zhao, X.H., Hu, Y.L., Liu, S.L., Nan, H.Y. et al. (2017). Natural variation at the soybean J locus improves adaptation to the tropics and enhances yield. Nature Genetics. 49: 773-779.
  10. Miranda, C., Scaboo, A., Cober, E., Denwar, N., Bilyeu, K. (2020). The effects and interaction of soybean maturity gene alleles controlling flowering time, maturity and adaptation in tropical environments. BMC Plant Biology. 20: 65.
  11. Neumaier, N. and James, A. (1993). Exploiting the long-juvenile trait to improve adaptation of soybeans to the tropics. Food Legume Newsletters. 8.
  12. Ray, J.D., Hinson, K., Mankono, J.E.B. and Malo, M.F.J.C.S. (1995). Genetic control of a long juvenile trait in soybean. Crop Science. 35: 1001-1006.
  13. Yang, W., Wu, T., Zhang, X., Song, W., Xu, C., Sun, S., et al (2019). Critical Photoperiod Measurement of Soybean Genotypes in Different Maturity Groups. Crop Science. 59: 2055-2061.
  14. Yue, Y.L., Liu, N.X., Jiang, B.J., Li, M., et al. (2017). A single nucleotide deletion in J encoding GmELF3 confers long juvenility and is associated with adaption of tropic soybean. Molecular Plant. 10: 656-658.

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