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Comparison of Biochemical Responses of Common Vetch (Vicia sativa L.) Seedling Organs to Salinity

DOI: 10.18805/LR-595    | Article Id: LR-595 | Page : 641-645
Citation :- Comparison of Biochemical Responses of Common Vetch (Vicia sativa L.) Seedling Organs to Salinity.Legume Research.2021.(44):641-645
Ramazan Beyaz ramazanbeyaz@gmail.com
Address : Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Kırşehir Ahi Evran University, 40100-Kırşehir, Turkey.
Submitted Date : 30-10-2020
Accepted Date : 25-01-2021

Abstract

Background: Shoots and roots are autotrophic and heterotrophic organs of plants with different physiological and biochemical functions under stress conditions. The metabolites involved in tolerance enhancement differed between roots and shoots. In this study, the biochemical changes occurring in shoot and root organs under salt stress and the level of these changes were investigated. However, these changes in shoot and root organs were compared.
Methods: Seeds of common vetch were sown and subjected to 14 days of salt stress in basal MS medium containing 100 mM NaCl. In shoot and root tissue, biochemical parameters such as antioxidant enzymes activities (GR, APX, SOD and CAT), malondialdehyde (MDA) content and proline accumulation were determined.
Result: Results of the study indicated that the activities of antioxidant enzymes (SOD, CAT (except in shoot), GR and APX), MDA and proline accumulation enhanced by salt stress in both organs. On the other hand, morphological parameters decreased in both tissues. It seemed that antioxidant enzyme activities more active in root tissues. However, proline accumulation was found higher in shoot tissues than root tissue, while MDA content was higher in root tissue than shoot tissue. The present investigation provides essential information for the antioxidant components of the shoot and root organs of vetch seedlings under salt stress.

Keywords

Antioxidant enzymes In vitro culture Malondialdehyde (MDA) Proline Salt (NaCl) stress

References

  1. Abdelgawad, H., Zinta, G., Hegab, M.M., Pandey, R., Asard, H., Abuelsoud, W. (2016). High salinity induces different oxidative stress and antioxidant responses in maize seedlings organs. Front Plant Sci. 7: 276.
  2. Abreu, I.A., Farinha, A.P., Negrão, S., Gonçalves, N., Fonseca, C., Rodrigues, M. (2013). Coping with abiotic stress: proteome changes for crop improvement. J. Proteom. 93: 145-168. 
  3. Ambede, J.G., Netondo, G.W., Mwai, G.N., Musyimi, D.M. (2012). NaCl salinity affects germination, growth, physiology and biochemistry of bambara groundnut. Br. J. Plant Physiol. 24: 151-160.
  4. Azevedo Neto, A.D., Prisco, J.T., En´Eas-Filho, J., Braga De Abreu, C.E., Gomes-Filho, E. (2006). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environ. Exp. Bot. 56: 87-94.
  5. Bandeoðlu, E., Eyidoggan, F., Yucel, M., Oktem, H.A. (2004). Antioxidant responses of shoots and roots of lentil to NaCl-salinity stress. Plant Growth Regul. 42: 69-77.
  6. Bates, L.S., Waldren, R.P., Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil. 39: 205-207.
  7. Beyaz, R., Yıldız, M. (2020). Influence of peg-induced drought stress on antioxidant components of callus tissue of sainfoin (Onobrychis viciifolia Scop.) ecotypes. Legume Res. online first article. DOI: 10.18805/LR-556
  8. Beyaz, R. (2019). Biochemical responses of shoot and root tissues of sainfoin (Onobrychis viciifolia Scop.) to NaCl-salt stress under in vitro conditions. Turkish Journal of Agriculture - Food Science and Technology. 7(1): 110-114.
  9. Beyaz, R. (2019). Biochemical responses of sainfoin shoot and root tissues to drought stress in in vitro culture. Legum. Res. 42(2) 2019: 173-177.
  10. Bilgili, U., Budakli Çarpici, E., Asik, B.B., Çelik, N. (2011). Root and shoot response of common vetch (Vicia Sativa L.), forage pea (Pisum Sativum L.) and canola (Brassica Napus L.) to salt Stress during early seedling growth stages. Turk. J. Fıeld Crops. 16(1): 33-38.
  11. Çakmak, I., Atli, M., Kaya, R., Evliya, H., Marschner, H. (1995). Association of high light and zinc deficiency in Cold-induced leaf chlorosis in grapefruit and mandarin trees. J. Plant Physiol. 146: 355-360.
  12. Cakmak, I., Marschner, H. (1992). Magnesium defficiency and higlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol. 98: 1222-1226.
  13. Desokya, E.S.M., Merwad, A.R.M., Rady, M.M. (2018). Natural biostimulants improve saline soil characteristics and salt stressed-sorghum performance. Commun Soil Sci. Plan. 49(8): 967-983.
  14. Dugasa, M.T., Cao, F., Ibrahim, W., Wu, F. (2019). Differences in physiological and biochemical characteristics in response to single and combined drought and salinity stresses between wheat senotypes differing in salt tolerance. Physiol. Plant. 165: 134-143.
  15. Ercan, O. (2008). Effect of drought and salt stresses on antioxidant defense system and physiology of lentil (Lens Culinaris M.) seedlings, Middle East Technical University (Turkey), Master Thesis (126 Pp.)
  16. Ertekin, I., Yilmaz, Ş., Atak, M., Can, E. (2018). Effects of different salt concentrations on the germination properties of hungarian vetch (Vicia Pannonica Crantz.) cultivars. Turkish Journal of Agricultural And Natural Sciences (Turkjans). 5(2): 175-179.
  17. Ertekin, I., Yilmaz, Ş., Atak, M., Can, E., Çeliktaş, N. (2017). Tuz Stresinin Bazı Yaygın Fiğ (Vicia Sativa L.) Çeşitlerinin Çimlenmesi Üzerine Etkileri, Journal of Agricultural Faculty of Mustafa Kemal University. 22(2):10-18.
  18. Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M.A. (2009). Plant drought stress: effects, mechanisms and management. In: Lichtfouse, E., Navarrete, M., Debaeke, P., Ve ´Ronique, S., Alberola, C. (Eds) Sustainable Agriculture. Pringer Netherlands. Pp: 185-212.
  19. Feierabend, J., Dehne, S. (1996). Fate of the porphyrin cofactors during the light-dependent turnover of catalase and of the photosystem Ii reaction-center protein D1 in mature rye leaves. Planta. 198: 413-422.
  20. Flowers, T., Gaur, P.M., Gowda, C.L.L., Krishnamurthy, L., Samineni, S., Siddique, K.H.M., Turner, N.C., Vadez, V., Varshney, R.K., Colmer, T.D. (2010). Salt sensitivity in chickpea. Plant Cell Environ. 33: 490-509.
  21. Kavas, M., Baloğlu, M.C., Akça, O., Köse, F.S., Gökçay, D. (2013). Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turk. J. Biol. 37: 491-498.
  22. Khan, M.H., Singha, K.L.B., Panda, S.K. (2002). Changes in antioxidant levels in (Oryza Sativa L.) roots subjected to NaCl salinity stress. Acta Physiol. Plant. 24: 145-148.
  23. Kusvuran, A. (2015). The effects of salt stress on the germination and antioxidative enzyme activity of hungarian vetch (Vicia Pannonica Crantz.) varieties. Legume Res. 38(1): 51-59.
  24. Lazof, D.B., Bernstein, N. (1999). The NaCl induced inhibition of shoot growth: the case for disturbed nutrition with special consideration of calcium. Adv. Bot. Res. 29: 113-189.
  25. Lutts, S., Kine, J.M., Bouharmont, J. (1996). NaCl-induced senesence in leaves of rice (Oryza Sativa L.) cultivars differing in salinity resistance. Annual Botany 78: 389-398.
  26. Munns, R., Tester, M. (2008). Mechanisms of salinity tolerance. Annu. Rev. Plant Biol. 59: 651-681.
  27. Murashıge, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
  28. Orak, A., Ateş, E. (2005). Resistance to salinity stress and available water levels at the seedling stage of the common vetch (Vicia sativa L.). Plant Soil Environ. 51(2): 51-56.
  29. Piwowarczyk, B., Tokarz, K., Kaminska, I. (2016). Responses of grass pea seedlings to salinity stress in in vitro culture conditions. Plant Cell Tiss Organ Cult. 124: 227-240.
  30. Polle, A., Eiblmeier, M., Sheppard, L., Murray, M. (1997). Responses of antioxidative enzymes to elevated Co2 in leaves of beech (Fagus Sylvatica L.) seedlings grown under a range of nutrient regimes, Plant Cell Environ. 20: 1317-1321.
  31. Talukdar, D. (2011). Flower and pod production, abortion, leaf injury, yield and seed neurotoxin levels in stable dwarf mutant lines of grass pea (Lathyrus Sativus L.) differing in salt stress responses. Int J. Curr Res. 2(1): 046-054.
  32. Tsegay, B.A., Gebreslassie, B. (2014). The effect of salinity (NaCl) on germination and early seedling growth of Lathyrus sativus and Pisum sativum Var. Abyssinicum. Afr. J. Plant Sci. 8(5): 225-23. 

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