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Research Article

volume 44 issue 2 (february 2021) : 197-201,   Doi: 10.18805/LR-556

Influence of Peg-induced Drought Stress on Antioxidant Components of Callus Tissue of Sainfoin (Onobrychis viciifolia Scop.) Ecotypes

R
Ramazan Beyaz1,*
M
Mustafa Yıldız2
1Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Kýrþehir Ahi Evran University, 40100, Kýrsehir, Turkey.
2Department of Field Crops, Faculty of Agriculture, Ankara University, 60100, Ankara, Turkey.

  • Submitted09-03-2020|

  • Accepted24-04-2020|

  • First Online 10-09-2020|

  • doi 10.18805/LR-556

Cite article:- Beyaz Ramazan, Yıldız Mustafa (2020). Influence of Peg-induced Drought Stress on Antioxidant Components of Callus Tissue of Sainfoin (Onobrychis viciifolia Scop.) Ecotypes . Legume Research. 44(2): 197-201. doi: 10.18805/LR-556.

ABSTRACT

Background: Drought is one of the important abiotic stress factors that restrict plant development. Sainfoin is known to be relatively tolerant of drought. However, there are limited reports on the effects of drought stress on antioxidant components of Onobrychis species and/or ecotypes. 

Methods: To determine drought stress effects on antioxidant components of 4 sainfoin ecotypes (“Koçaþ”, “Malya”, “Altýnova” and “Ulaþ”), callus tissue was grown on MS medium enriched with 200 g l-1 PEG-6000. 

Result: Callus of the sainfoin ecotypes, cultured on a medium having 200 g l-1 PEG-6000, showed significant increases in antioxidant enzyme activities (SOD, CAT, GR (except in “Ulaþ” ecotype) and APX). However, the PEG induced increase in the accumulation of MDA and proline in callus tissue of all sainfoin ecotypes. The findings of the present study show that in terms of the increasing rate of antioxidant components under drought stress, the “Koçaþ” ecotype seemed to be the best.

REFERENCES


  1. Atkinson, N.J. and, Urwin, P.E. (2012). The interaction of plant biotic and abiotic stresses: from From genes to the field. Journal of Experimental Botany. 63: 3523-3544.

  2. Bates, L.S., Waldren, R.P., Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil. 39: 205-207.

  3. Beyaz, R. (2019). Biochemical responses of sainfoin shoot and root tissues to drought stress in in vitro culture. Legume Research. 42(2): 173-177.

  4. Beyaz, R., Sancak, C., Yýldýz, M. (2018). Morphological and biochemical responses of sainfoin (Onobrychis viciifolia Scop.) ecotypes to salinity. Legume Research. 41(2): 253-258.

  5. Bezirðanoðlu, I. (2017). Response of five triticale genotypes to salt stress in in vitro culture. Turkish Journal of Agriculture and Forestry. 41: 372-380.

  6. Carbonero, C.H., Mueller-Harvey, I., Brown, T.A., Smith, L. (2011). Sainfoin (Onobrychis viciifolia): a A beneficial forage legume. Plant Genetic Resources. 9(1): 70-85.

  7. Çakmak, I. and Marschner, H. (1992). Magnesium Defficiency and Higlight Ýntensity Enhance Activities of Superoxide Dismutase, Ascorbate Peroxidase and Glutathione Reductase in Bean Leaves. Plant Physiology. 98: 1222-1226.

  8. Çakmak, I., Atlý, M., Kaya, R., Evliya, H., Marschner, H. (1995). Association of High Light and Zinc Deficiency in Cold-Induced Leaf Chlorosis in Grapefruit and Mandarin Trees. The Journal of Plant Physiology. 146: 355-360.

  9. Dalton, R.G., Eddie, P.M., Cran, L., Stephen, W.B., Marye-Michelle, M. (1994). The effects of NaCI on antioxidant enzyme activities in callus tissue of salt-tolerant and salt-sensitive cotton cultivars (Gossypium hirsutum L.). Plant Cell Reports. 13: 498-503.

  10. Das, K. and, Roychoudhury, A. (2014). Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science. 2: 53.

  11. Ehsanpour, A.A. and Fatahian, N. (2003). Effects of salt and proline on Medicago sativa callus. Plant Cell, Tissue and Organ Culture. 73: 53-56.

  12. Fikret, Y., Manar, T., Þebnem, E., Þebnem, K., Özlem, U. (2013). SOD, CAT, GR and APX Enzyme Activities in Callus Tissues of Susceptible and Tolerant Eggplant Varieties under Salt Stress. Research Journal of Biotechnology. 8(11): 45-50. 

  13. Garshasbi, H., Omidi, M., Torabý, S., Davodi, D. (2012). The study of phytohormones and explants on callus induction and regeneration of sainfoin (Onobrychis sativa). Pakistan Journal of Agricultural Sciences. 49: 319-322.

  14. Gill, S.S. and, Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry. 48: 909-930.

  15. Irani, S., Majidi, M.M., Mirlohi, A., Zargar, M., Karami, M. (2015). Assessment of Drought Tolerance in Sainfoin: Physiological and Drought Tolerance Indices. Agronomy Journal. 107(5): 1171-1781.

  16. Kusvuran, S., Ellialtýoðlu, S., Polat, Z. (2013). Antioxidative Enzyme Activity, Lipid Peroxidation and Proline Accumulation in the Callus Tissues of Salt and Drought Tolerant and Sensitive Pumpkin Genotypes under Chilling Stress. Horticulture Environment and Biotechnology. 54(4): 319-325.

  17. Kuþvuran, Þ., Ellialtýoglu Þ.Þ., Talhouni, M., Sonmez, K., Kýran, S. (2016). Effects of salt and drought stresses on physiological and biochemical changes in callus tissues of melon cultivars. Acta Horticulturae. 1142: 236-246.

  18. Liang, X., Zhang, L., Natarajan, S.K., Becker, D.F. (2013). Proline Mechanisms of Stress Survival. Antioxidants and Redox Signaling. 19(9): 998-1011.

  19. Libik, M., Konieczny, R., Pater, B, S´Lesak, I., Miszalski, Z. (2005). Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant. Plant Cell Reports. 23: 834-841.

  20. Lutts, S., Kine, J.M., Bouharmont, J. (1996). NaCl-Induced Senesence in Leaves of Rice (Oryza sativa L.) Cultivars Differing in Salinity Resistance. Annals of Botany. 78: 389-398.

  21. Mittler, R. (2017). ROS are good. Trends in Plant Science. 22: 11-19.

  22. Mittler, R., Vanderauwera, S., Gollery, M., Van Breusegem, F. (2004). Reactive oxygen gene network of plants. Trends in Plant Science. 9: 490-498.

  23. Munir, N. and, Aftab, F. (2013). Changes in Activities of Antioxidant Enzymes in Response to NaCl Stress in Callus Cultures and Regenerated Plants of Sugarcane. Journal of Animal and Plant Sciences. 23(1): 203-209.

  24. Murashige, T. and, Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum.15(3):473-497.

  25. Namjooyan, H., Khavari-Nejad, R., Bernard, F., Namdjoyan, S., Piri H. (2012). The effect of cadmium on growth and antioxidant responses in the saffl ower (Carthamus tinctorius L.) callus. Turkish Journal of Agriculture and Forestry. 36: 145-152.

  26. Niknam, V., Meratan, A.A., Ghaffari, S.M. (2011). The effect of salt stress on lipid peroxidation and antioxidative enzymes in callus of two Acanthophyllum species Th effect of cadmium on growth and antioxidant responses in the saffl ower (Carthamus tinctorius L.) callus. In Vitro Cellular and Developmental Biology. 47: 297-308.

  27. Noctor, G., Mhamdi, A., Foyer, C.H. (2014). The roles of reactive oxygen metabolism in drought: not Not so cut and dried. Plant Physiology. 164: 1636-1648.

  28. Özcan, S., Gürel, E., Babaoðlu, M. (2004). Plant Biotechnology (Genetic Engineering and Applications). Selçuk University Press (In Turkey).

  29. Revathi, S. and Pillai, M. A. (2015). Novel approach in screening rice genotype for tolerance to salt stress under hydroponic culture. Indian Journal of Agricultural Research. 35(4): 308-310.

  30. Safarnejad, A. (2004). Characterization of Somaclones of Medicago sativa L. for Drought Tolerance. Journal of Agricultural Science and Technology. 6: 121-127.

  31. Schneider, J.R., Caverzan, A., Chavarria, G. (2019). Water deficit stress, ROS involvement and plant performance. Archives of Agronomy and Soil Science. 65(8):1160-1181.
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    Research Article

    volume 44 issue 2 (february 2021) : 197-201,   Doi: 10.18805/LR-556

    Influence of Peg-induced Drought Stress on Antioxidant Components of Callus Tissue of Sainfoin (Onobrychis viciifolia Scop.) Ecotypes

    R
    Ramazan Beyaz1,*
    M
    Mustafa Yıldız2
    1Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Kýrþehir Ahi Evran University, 40100, Kýrsehir, Turkey.
    2Department of Field Crops, Faculty of Agriculture, Ankara University, 60100, Ankara, Turkey.

    • Submitted09-03-2020|

    • Accepted24-04-2020|

    • First Online 10-09-2020|

    • doi 10.18805/LR-556

    Cite article:- Beyaz Ramazan, Yıldız Mustafa (2020). Influence of Peg-induced Drought Stress on Antioxidant Components of Callus Tissue of Sainfoin (Onobrychis viciifolia Scop.) Ecotypes . Legume Research. 44(2): 197-201. doi: 10.18805/LR-556.

    ABSTRACT

    Background: Drought is one of the important abiotic stress factors that restrict plant development. Sainfoin is known to be relatively tolerant of drought. However, there are limited reports on the effects of drought stress on antioxidant components of Onobrychis species and/or ecotypes. 

    Methods: To determine drought stress effects on antioxidant components of 4 sainfoin ecotypes (“Koçaþ”, “Malya”, “Altýnova” and “Ulaþ”), callus tissue was grown on MS medium enriched with 200 g l-1 PEG-6000. 

    Result: Callus of the sainfoin ecotypes, cultured on a medium having 200 g l-1 PEG-6000, showed significant increases in antioxidant enzyme activities (SOD, CAT, GR (except in “Ulaþ” ecotype) and APX). However, the PEG induced increase in the accumulation of MDA and proline in callus tissue of all sainfoin ecotypes. The findings of the present study show that in terms of the increasing rate of antioxidant components under drought stress, the “Koçaþ” ecotype seemed to be the best.

    REFERENCES


    1. Atkinson, N.J. and, Urwin, P.E. (2012). The interaction of plant biotic and abiotic stresses: from From genes to the field. Journal of Experimental Botany. 63: 3523-3544.

    2. Bates, L.S., Waldren, R.P., Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil. 39: 205-207.

    3. Beyaz, R. (2019). Biochemical responses of sainfoin shoot and root tissues to drought stress in in vitro culture. Legume Research. 42(2): 173-177.

    4. Beyaz, R., Sancak, C., Yýldýz, M. (2018). Morphological and biochemical responses of sainfoin (Onobrychis viciifolia Scop.) ecotypes to salinity. Legume Research. 41(2): 253-258.

    5. Bezirðanoðlu, I. (2017). Response of five triticale genotypes to salt stress in in vitro culture. Turkish Journal of Agriculture and Forestry. 41: 372-380.

    6. Carbonero, C.H., Mueller-Harvey, I., Brown, T.A., Smith, L. (2011). Sainfoin (Onobrychis viciifolia): a A beneficial forage legume. Plant Genetic Resources. 9(1): 70-85.

    7. Çakmak, I. and Marschner, H. (1992). Magnesium Defficiency and Higlight Ýntensity Enhance Activities of Superoxide Dismutase, Ascorbate Peroxidase and Glutathione Reductase in Bean Leaves. Plant Physiology. 98: 1222-1226.

    8. Çakmak, I., Atlý, M., Kaya, R., Evliya, H., Marschner, H. (1995). Association of High Light and Zinc Deficiency in Cold-Induced Leaf Chlorosis in Grapefruit and Mandarin Trees. The Journal of Plant Physiology. 146: 355-360.

    9. Dalton, R.G., Eddie, P.M., Cran, L., Stephen, W.B., Marye-Michelle, M. (1994). The effects of NaCI on antioxidant enzyme activities in callus tissue of salt-tolerant and salt-sensitive cotton cultivars (Gossypium hirsutum L.). Plant Cell Reports. 13: 498-503.

    10. Das, K. and, Roychoudhury, A. (2014). Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science. 2: 53.

    11. Ehsanpour, A.A. and Fatahian, N. (2003). Effects of salt and proline on Medicago sativa callus. Plant Cell, Tissue and Organ Culture. 73: 53-56.

    12. Fikret, Y., Manar, T., Þebnem, E., Þebnem, K., Özlem, U. (2013). SOD, CAT, GR and APX Enzyme Activities in Callus Tissues of Susceptible and Tolerant Eggplant Varieties under Salt Stress. Research Journal of Biotechnology. 8(11): 45-50. 

    13. Garshasbi, H., Omidi, M., Torabý, S., Davodi, D. (2012). The study of phytohormones and explants on callus induction and regeneration of sainfoin (Onobrychis sativa). Pakistan Journal of Agricultural Sciences. 49: 319-322.

    14. Gill, S.S. and, Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry. 48: 909-930.

    15. Irani, S., Majidi, M.M., Mirlohi, A., Zargar, M., Karami, M. (2015). Assessment of Drought Tolerance in Sainfoin: Physiological and Drought Tolerance Indices. Agronomy Journal. 107(5): 1171-1781.

    16. Kusvuran, S., Ellialtýoðlu, S., Polat, Z. (2013). Antioxidative Enzyme Activity, Lipid Peroxidation and Proline Accumulation in the Callus Tissues of Salt and Drought Tolerant and Sensitive Pumpkin Genotypes under Chilling Stress. Horticulture Environment and Biotechnology. 54(4): 319-325.

    17. Kuþvuran, Þ., Ellialtýoglu Þ.Þ., Talhouni, M., Sonmez, K., Kýran, S. (2016). Effects of salt and drought stresses on physiological and biochemical changes in callus tissues of melon cultivars. Acta Horticulturae. 1142: 236-246.

    18. Liang, X., Zhang, L., Natarajan, S.K., Becker, D.F. (2013). Proline Mechanisms of Stress Survival. Antioxidants and Redox Signaling. 19(9): 998-1011.

    19. Libik, M., Konieczny, R., Pater, B, S´Lesak, I., Miszalski, Z. (2005). Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant. Plant Cell Reports. 23: 834-841.

    20. Lutts, S., Kine, J.M., Bouharmont, J. (1996). NaCl-Induced Senesence in Leaves of Rice (Oryza sativa L.) Cultivars Differing in Salinity Resistance. Annals of Botany. 78: 389-398.

    21. Mittler, R. (2017). ROS are good. Trends in Plant Science. 22: 11-19.

    22. Mittler, R., Vanderauwera, S., Gollery, M., Van Breusegem, F. (2004). Reactive oxygen gene network of plants. Trends in Plant Science. 9: 490-498.

    23. Munir, N. and, Aftab, F. (2013). Changes in Activities of Antioxidant Enzymes in Response to NaCl Stress in Callus Cultures and Regenerated Plants of Sugarcane. Journal of Animal and Plant Sciences. 23(1): 203-209.

    24. Murashige, T. and, Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum.15(3):473-497.

    25. Namjooyan, H., Khavari-Nejad, R., Bernard, F., Namdjoyan, S., Piri H. (2012). The effect of cadmium on growth and antioxidant responses in the saffl ower (Carthamus tinctorius L.) callus. Turkish Journal of Agriculture and Forestry. 36: 145-152.

    26. Niknam, V., Meratan, A.A., Ghaffari, S.M. (2011). The effect of salt stress on lipid peroxidation and antioxidative enzymes in callus of two Acanthophyllum species Th effect of cadmium on growth and antioxidant responses in the saffl ower (Carthamus tinctorius L.) callus. In Vitro Cellular and Developmental Biology. 47: 297-308.

    27. Noctor, G., Mhamdi, A., Foyer, C.H. (2014). The roles of reactive oxygen metabolism in drought: not Not so cut and dried. Plant Physiology. 164: 1636-1648.

    28. Özcan, S., Gürel, E., Babaoðlu, M. (2004). Plant Biotechnology (Genetic Engineering and Applications). Selçuk University Press (In Turkey).

    29. Revathi, S. and Pillai, M. A. (2015). Novel approach in screening rice genotype for tolerance to salt stress under hydroponic culture. Indian Journal of Agricultural Research. 35(4): 308-310.

    30. Safarnejad, A. (2004). Characterization of Somaclones of Medicago sativa L. for Drought Tolerance. Journal of Agricultural Science and Technology. 6: 121-127.

    31. Schneider, J.R., Caverzan, A., Chavarria, G. (2019). Water deficit stress, ROS involvement and plant performance. Archives of Agronomy and Soil Science. 65(8):1160-1181.
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