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Research Article

volume 43 issue 5 (october 2020) : 706-710,   Doi: 10.18805/LR-514

Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation

S
Snežana Andjelković
J
Jasmina Radović
S
Snežana Babić
T
Tanja Vasić
S
Simonida Djurić
D
Dragana Stamenov
T
Timea Hajnal Jafari
1Institute for forage crops Kruševac, 37251 Globoder, Republic of Serbia.

  • Submitted03-07-2019|

  • Accepted02-09-2020|

  • First Online 15-10-2020|

  • doi 10.18805/LR-514

Cite article:- Andjelković Snežana, Radović Jasmina, Babić Snežana, Vasić Tanja, Djurić Simonida, Stamenov Dragana, Jafari Hajnal Timea (2020). Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation. Legume Research. 43(5): 706-710. doi: 10.18805/LR-514.

ABSTRACT

Background: Various microorganisms that can have a positive or negative effect on plant development are present in the rhizospheric soil of alfalfa. The research aimed to investigate the impact of two nitrogen-fixing bacteria and two species of the phytopathogen fungus Colletotrichum on the abundance of aminoheterotrophs, nitrifying and denitrifying bacteria in the rhizosphere of three cultivars of alfalfa.
Methods: The experiment was carried in vegetation pots as three factorial, where the first factor was alfalfa cultivar (Affinity+Z, K-28 and Perry), the second was the isolate of phytopathogen fungus Colletotrichum: C. trifolii (isolate Coll 4) and C. destructivum (two isolates: Coll-11 and Coll 657); and the third was the variant of bacterial inoculation (Azotobacter chroococcum and Sinorhizobium meliloti). The number of microorganisms was determined by introducing a diluted soil suspension into proper media and counted per one gram of absolutely dry soil.
Result: According to the Fisher test applied inoculation microbial inoculation with nitrogen-fixing bacteria and phytopathogen fungus had different effects on the abundance of examined microorganisms in rizospheric soil of different alfalfa cultivars. 

REFERENCES

  1. Achouak, W., Abrouk, D., Guyonnet, J., Barakat, M., Ortet, P., Simon, l., Lerondelle, C., Heulin, T. and Haichar, F.Z. (2019). Plant hosts control microbial denitrification activity. FEMS Microbiology Ecology. 95(3): fiz021.
  2. Berg, G, (2009). Plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Applied Microbiology and Biotechnology. 84 (1): 11-8. 
  3. Doolotkeldieva, T., Bobusheva, S. and Konurbaeva, M (2015). Effects of Streptomyces biofertilizer to soil fertility and rhizosphere’s functional biodiversity of agricultural plants. Advances in Microbiology. 5: 555-571.
  4. Dey, R., Sarkar, K., Dutta, S., Murmu, S. and Mandal, N. (2017). Role of Azotobacter sp. isolates as a plant growth promoting agent and their antagonistic potentiality against soil borne pathogen (Rhizoctonia solani) under in vitro condition. Int. J. Curr. Microbiol. App. Sci. 6 (11): 2830-2836.
  5. Girija, D., Panchami, P.S., Jose, P.E. Saeed T. and Nair, S.S. (2020). Isolation and characterization of native cowpea rhizobia from Wayanad India. Legume Research.(43): 126-133.
  6. Gul, A., Salam, A., Afridi, M.S., Bangash, N.k., Ali, F., Ali, M.Y., Khan, S. and Mubeeen, R. (2019). Effect of urea, bio-fertilizers and their interaction on the growth, yield and yield attributes of Cyamopsis Tetragonoloba. Indian Journal of Agricultural Research. 53(4): 423-428 .
  7. Hayat, R., Ali. S., Amara, U., Khalid, R. and Ahmed, I. (2010). Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol., 60: 579-598. 
  8. Hrynkiewicz, K. and Baum, C. (2011). The potential of rhizosphere microorganisms to promote the plant growth in disturbed soils. Malik A & Grohmann E (eds.), Environmental Protection Strategies for Sustainable Development, Strategies for Sustainability. Springer: 35-64.
  9. Huang, S., Chen, C., Yang, X., Wu, Q. and Zhang, R. (2011). Distribution of typical denitrifying functional genes and diversity of the nirS-encoding bacterial community related to environmental characteristics of river sediments. Biogeosciences. 8: 3041-3051. 
  10. Jarak, M. and Djuriæ S. (2006). Practicum of Microbiology. Faculty of Agriculture, Novi Sad.
  11. Karayilanli, E. and Ayhan, V. (2016). Investigation of feed value of alfalfa (Medicago sativa L.) harvested at different maturity stages. Legume Research. 39 (2): 237-247.
  12. Koleško, O.J. (1981). Ekoligija mikrooraganizmov poèvi. Višaja škola, Minks.
  13. Kumar, H., Dubey, R.C. and Maheshwari, D.K. (2011). Effect of plant growth promoting rhizobia on seed germination, growth promotion and suppression of Fusarium wilt of fenugreek (Trigonella foenum-graecum L.). Crop Protection 30 (11): 1396-1403.
  14. Liu, L., Li Z., Shi, W., Li, H., Huang, F., Li, J. and Wang M. (2016). Study on the taxonomy status of alfalfa with cream flowers. Legume Research. 39 (6): 860-867.
  15. Majumder, S.P., Chaitanya, A.K., Datta, A., Padhan, D., Badole, S. and Mandai, B. (2018) Dynamics of carbon and nitrogen in agricultural soils: role of organic and inorganic sources. In: María Ángeles Muñoz and Raúl Zornoza eds. Soil Management and Climate Change. Elsevier Academic Press: 151-169.
  16. Meena, S.S., Mehta, R.S, Bairwa, M. and Meena, R.D. (2014). productivity and profitability of fenugreek (Trigonella foenum-graecum l.) As influenced by bio-fertilizers and plant growth regulators. Legume Research. 37 (6): 646-650.
  17. Mrkovaèki, N., Bjeliæ, D., Maksimoviæ, L., Æurèiæ, Ž., Æiriæ, M., Živanov, M. (2016). The effect of inoculation with Azotobacter chroococcum on microorganisms in rhizosphere and sugar beet yield in organic farming. Matica Srpska Journal for Natural Sciences. 130: 45-52.
  18. Nath, D.J., Gogoi, D., Gayan, A. and Chelleng, A. (2015). Influence of chemical and biological properties of field grown pea and lentil rhizospheres on nodulation status and resident rhizobial population in acidic soils of Assam, India. Legume Research. 38 (6): 844-850.
  19. Poshon, J. and Tardieux, P.(1962): Tehniques d’analise en microbiologie du sol, Paris.
  20. Reinhold-Hurek, B. and Hurek T. (2011). Living inside plants: bacterial endophytes. Curr. Opin. Plant Biol. 14: 435-443.
  21. Sahrawat, K.L, (2008). Factors affecting nitrification in soils. Communications in Soil Science and Plant Analysis. 39 (9-10): 1436-1446. 
  22. Skiba, W.M., George, T.S., Baggs, E.M. and Daniell, T.J. (2011). Plant influence on nitrification. Biochem Soc Trans. 39 (1): 275-278. 
  23. Stamenov, D., Djuriæ. S., Hajnal-Jafari. T. and Andjelkoviæ, S. (2017). Influence of Pseudomonas and Bacillus strains isolated from Lolium perenne rhizospheric soil in Vojvodina (Serbia) on planth growth and soil microbial communities. Polish Journal of Microbiology. 66 (2): 269-272.
  24. Strbanoviæ, R., Simic, A., Postic, D., Zivanovic, T., Vuckovic, S., Pfaf-Dolovac, E. and Stanisavljevic, R. (2015). Yield and morphological traits in alfalfa varieties of different origin. Legume Research. 38 (4): 434-441.
  25. Stres, B., Danevèeviæ, T., Pal, L., Mrkonjic-Fuka, M., Resman, L., Leskovec, S., Hacin, J., Stopar, D., Mahne, I. and Mandic-Mulec, I. (2008). Influence of temperature and soil water content on bacterial, archaeal and denitrifying microbial communities in drained fen grassland soil microcosms. FEMS Microbiology Ecology. 66: 110-122. 
  26. Subbarao, G.V., Tomohiro, B., Masahiro, K., Osamu, I., Samejima, H., Wang, H.Y., Pearse, S.J., Gopalakrishnan, S., Nakahara, K., Zakir Hossain, A.K M., Tsujimoto, H. and Berry, W.L. (2007). Can biological nitrification inhibition (BNI) genes from perennial Leymus racemosus (Triticeae) combat nitrification in wheat farming? Plant Soil. 299: 55-64.
  27. Vasiæ, T., Anðelkoviæ, S. , Radoviæ, J., Lugiæ, Z., Hajnal-Jafari, T., Djuræ, S. and Živkoviæ, S. (2014). Alfalfa inoculation: the effect on root growth and number of rhizospheric microorganisms. Romanian Biotechnological Letters. 19(4): 9457-9464.
  28. Vasiæ, T., Radoviæ, J., Lugiæ, Z., Markoviæ, J., Jevtiæ, G. and Gajiæ, S. (2009). Occurrence of C. trifolii (Bain et Essary), the inducer of alfalfa anthracnose in Serbia. In: Sustainable use of genetic diversity in forage and turf breeding, [I C Huyghe (ed)]. Springer: 369-374. 
  29. Yadav, B.K. (2015).Variation in soil biological properties with legume crops in arid region of Rajasthan, India. Legume Research. 38 (6): 772-780.
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    Research Article

    volume 43 issue 5 (october 2020) : 706-710,   Doi: 10.18805/LR-514

    Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation

    S
    Snežana Andjelković
    J
    Jasmina Radović
    S
    Snežana Babić
    T
    Tanja Vasić
    S
    Simonida Djurić
    D
    Dragana Stamenov
    T
    Timea Hajnal Jafari
    1Institute for forage crops Kruševac, 37251 Globoder, Republic of Serbia.

    • Submitted03-07-2019|

    • Accepted02-09-2020|

    • First Online 15-10-2020|

    • doi 10.18805/LR-514

    Cite article:- Andjelković Snežana, Radović Jasmina, Babić Snežana, Vasić Tanja, Djurić Simonida, Stamenov Dragana, Jafari Hajnal Timea (2020). Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation. Legume Research. 43(5): 706-710. doi: 10.18805/LR-514.

    ABSTRACT

    Background: Various microorganisms that can have a positive or negative effect on plant development are present in the rhizospheric soil of alfalfa. The research aimed to investigate the impact of two nitrogen-fixing bacteria and two species of the phytopathogen fungus Colletotrichum on the abundance of aminoheterotrophs, nitrifying and denitrifying bacteria in the rhizosphere of three cultivars of alfalfa.
    Methods: The experiment was carried in vegetation pots as three factorial, where the first factor was alfalfa cultivar (Affinity+Z, K-28 and Perry), the second was the isolate of phytopathogen fungus Colletotrichum: C. trifolii (isolate Coll 4) and C. destructivum (two isolates: Coll-11 and Coll 657); and the third was the variant of bacterial inoculation (Azotobacter chroococcum and Sinorhizobium meliloti). The number of microorganisms was determined by introducing a diluted soil suspension into proper media and counted per one gram of absolutely dry soil.
    Result: According to the Fisher test applied inoculation microbial inoculation with nitrogen-fixing bacteria and phytopathogen fungus had different effects on the abundance of examined microorganisms in rizospheric soil of different alfalfa cultivars. 

    REFERENCES

    1. Achouak, W., Abrouk, D., Guyonnet, J., Barakat, M., Ortet, P., Simon, l., Lerondelle, C., Heulin, T. and Haichar, F.Z. (2019). Plant hosts control microbial denitrification activity. FEMS Microbiology Ecology. 95(3): fiz021.
    2. Berg, G, (2009). Plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Applied Microbiology and Biotechnology. 84 (1): 11-8. 
    3. Doolotkeldieva, T., Bobusheva, S. and Konurbaeva, M (2015). Effects of Streptomyces biofertilizer to soil fertility and rhizosphere’s functional biodiversity of agricultural plants. Advances in Microbiology. 5: 555-571.
    4. Dey, R., Sarkar, K., Dutta, S., Murmu, S. and Mandal, N. (2017). Role of Azotobacter sp. isolates as a plant growth promoting agent and their antagonistic potentiality against soil borne pathogen (Rhizoctonia solani) under in vitro condition. Int. J. Curr. Microbiol. App. Sci. 6 (11): 2830-2836.
    5. Girija, D., Panchami, P.S., Jose, P.E. Saeed T. and Nair, S.S. (2020). Isolation and characterization of native cowpea rhizobia from Wayanad India. Legume Research.(43): 126-133.
    6. Gul, A., Salam, A., Afridi, M.S., Bangash, N.k., Ali, F., Ali, M.Y., Khan, S. and Mubeeen, R. (2019). Effect of urea, bio-fertilizers and their interaction on the growth, yield and yield attributes of Cyamopsis Tetragonoloba. Indian Journal of Agricultural Research. 53(4): 423-428 .
    7. Hayat, R., Ali. S., Amara, U., Khalid, R. and Ahmed, I. (2010). Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol., 60: 579-598. 
    8. Hrynkiewicz, K. and Baum, C. (2011). The potential of rhizosphere microorganisms to promote the plant growth in disturbed soils. Malik A & Grohmann E (eds.), Environmental Protection Strategies for Sustainable Development, Strategies for Sustainability. Springer: 35-64.
    9. Huang, S., Chen, C., Yang, X., Wu, Q. and Zhang, R. (2011). Distribution of typical denitrifying functional genes and diversity of the nirS-encoding bacterial community related to environmental characteristics of river sediments. Biogeosciences. 8: 3041-3051. 
    10. Jarak, M. and Djuriæ S. (2006). Practicum of Microbiology. Faculty of Agriculture, Novi Sad.
    11. Karayilanli, E. and Ayhan, V. (2016). Investigation of feed value of alfalfa (Medicago sativa L.) harvested at different maturity stages. Legume Research. 39 (2): 237-247.
    12. Koleško, O.J. (1981). Ekoligija mikrooraganizmov poèvi. Višaja škola, Minks.
    13. Kumar, H., Dubey, R.C. and Maheshwari, D.K. (2011). Effect of plant growth promoting rhizobia on seed germination, growth promotion and suppression of Fusarium wilt of fenugreek (Trigonella foenum-graecum L.). Crop Protection 30 (11): 1396-1403.
    14. Liu, L., Li Z., Shi, W., Li, H., Huang, F., Li, J. and Wang M. (2016). Study on the taxonomy status of alfalfa with cream flowers. Legume Research. 39 (6): 860-867.
    15. Majumder, S.P., Chaitanya, A.K., Datta, A., Padhan, D., Badole, S. and Mandai, B. (2018) Dynamics of carbon and nitrogen in agricultural soils: role of organic and inorganic sources. In: María Ángeles Muñoz and Raúl Zornoza eds. Soil Management and Climate Change. Elsevier Academic Press: 151-169.
    16. Meena, S.S., Mehta, R.S, Bairwa, M. and Meena, R.D. (2014). productivity and profitability of fenugreek (Trigonella foenum-graecum l.) As influenced by bio-fertilizers and plant growth regulators. Legume Research. 37 (6): 646-650.
    17. Mrkovaèki, N., Bjeliæ, D., Maksimoviæ, L., Æurèiæ, Ž., Æiriæ, M., Živanov, M. (2016). The effect of inoculation with Azotobacter chroococcum on microorganisms in rhizosphere and sugar beet yield in organic farming. Matica Srpska Journal for Natural Sciences. 130: 45-52.
    18. Nath, D.J., Gogoi, D., Gayan, A. and Chelleng, A. (2015). Influence of chemical and biological properties of field grown pea and lentil rhizospheres on nodulation status and resident rhizobial population in acidic soils of Assam, India. Legume Research. 38 (6): 844-850.
    19. Poshon, J. and Tardieux, P.(1962): Tehniques d’analise en microbiologie du sol, Paris.
    20. Reinhold-Hurek, B. and Hurek T. (2011). Living inside plants: bacterial endophytes. Curr. Opin. Plant Biol. 14: 435-443.
    21. Sahrawat, K.L, (2008). Factors affecting nitrification in soils. Communications in Soil Science and Plant Analysis. 39 (9-10): 1436-1446. 
    22. Skiba, W.M., George, T.S., Baggs, E.M. and Daniell, T.J. (2011). Plant influence on nitrification. Biochem Soc Trans. 39 (1): 275-278. 
    23. Stamenov, D., Djuriæ. S., Hajnal-Jafari. T. and Andjelkoviæ, S. (2017). Influence of Pseudomonas and Bacillus strains isolated from Lolium perenne rhizospheric soil in Vojvodina (Serbia) on planth growth and soil microbial communities. Polish Journal of Microbiology. 66 (2): 269-272.
    24. Strbanoviæ, R., Simic, A., Postic, D., Zivanovic, T., Vuckovic, S., Pfaf-Dolovac, E. and Stanisavljevic, R. (2015). Yield and morphological traits in alfalfa varieties of different origin. Legume Research. 38 (4): 434-441.
    25. Stres, B., Danevèeviæ, T., Pal, L., Mrkonjic-Fuka, M., Resman, L., Leskovec, S., Hacin, J., Stopar, D., Mahne, I. and Mandic-Mulec, I. (2008). Influence of temperature and soil water content on bacterial, archaeal and denitrifying microbial communities in drained fen grassland soil microcosms. FEMS Microbiology Ecology. 66: 110-122. 
    26. Subbarao, G.V., Tomohiro, B., Masahiro, K., Osamu, I., Samejima, H., Wang, H.Y., Pearse, S.J., Gopalakrishnan, S., Nakahara, K., Zakir Hossain, A.K M., Tsujimoto, H. and Berry, W.L. (2007). Can biological nitrification inhibition (BNI) genes from perennial Leymus racemosus (Triticeae) combat nitrification in wheat farming? Plant Soil. 299: 55-64.
    27. Vasiæ, T., Anðelkoviæ, S. , Radoviæ, J., Lugiæ, Z., Hajnal-Jafari, T., Djuræ, S. and Živkoviæ, S. (2014). Alfalfa inoculation: the effect on root growth and number of rhizospheric microorganisms. Romanian Biotechnological Letters. 19(4): 9457-9464.
    28. Vasiæ, T., Radoviæ, J., Lugiæ, Z., Markoviæ, J., Jevtiæ, G. and Gajiæ, S. (2009). Occurrence of C. trifolii (Bain et Essary), the inducer of alfalfa anthracnose in Serbia. In: Sustainable use of genetic diversity in forage and turf breeding, [I C Huyghe (ed)]. Springer: 369-374. 
    29. Yadav, B.K. (2015).Variation in soil biological properties with legume crops in arid region of Rajasthan, India. Legume Research. 38 (6): 772-780.
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