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Legume Research
Print ISSN: 0250-5371
Online ISSN: 0976-0571
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6.80
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Chief Editor:
J. S. Sandhu
Vice Chancellor, SKN Agriculture, University, Jobner, VC, NDUAT, Faizabad, Deputy Director General (Crop Science), Indian Council of Agricultural Research (ICAR), New Delhi
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Research Article

Legume Research, volume 43 issue 5 (october 2020) : 706-710

Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation

Snežana Andjelković, Jasmina Radović, Snežana Babić, Tanja Vasić, Simonida Djurić, Dragana Stamenov, Timea Hajnal Jafari
1Institute for forage crops Kruševac, 37251 Globoder, Republic of Serbia.

  • Submitted03-07-2019|

  • Accepted02-09-2020|

  • First Online 15-10-2020|

  • doi 10.18805/LR-514

Cite article:- Andjelković Snežana, Radović Jasmina, Babić Snežana, Vasić Tanja, Djurić Simonida, Stamenov Dragana, Jafari Hajnal Timea (2020). Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation. Legume Research. 43(5): 706-710. doi: 10.18805/LR-514.

ABSTRACT

Background: Various microorganisms that can have a positive or negative effect on plant development are present in the rhizospheric soil of alfalfa. The research aimed to investigate the impact of two nitrogen-fixing bacteria and two species of the phytopathogen fungus Colletotrichum on the abundance of aminoheterotrophs, nitrifying and denitrifying bacteria in the rhizosphere of three cultivars of alfalfa.
Methods: The experiment was carried in vegetation pots as three factorial, where the first factor was alfalfa cultivar (Affinity+Z, K-28 and Perry), the second was the isolate of phytopathogen fungus Colletotrichum: C. trifolii (isolate Coll 4) and C. destructivum (two isolates: Coll-11 and Coll 657); and the third was the variant of bacterial inoculation (Azotobacter chroococcum and Sinorhizobium meliloti). The number of microorganisms was determined by introducing a diluted soil suspension into proper media and counted per one gram of absolutely dry soil.
Result: According to the Fisher test applied inoculation microbial inoculation with nitrogen-fixing bacteria and phytopathogen fungus had different effects on the abundance of examined microorganisms in rizospheric soil of different alfalfa cultivars. 

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