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Research Article

volume 43 issue 4 (august 2020) : 512-517,   Doi: 10.18805/LR-491

Raffinose family oligosaccharides in seeds of common vetch (Vicia sativa L. ssp. sativa)

L
L.B. Lahuta
W
W. Rybiñski
J
J. Bocianowski
K
K. Nowosad
A
A. Börner
1Department of Plant Physiology, University of Warmia and Mazury in Olsztyn, Genetics and Biotechnology, Oczapowskiego 1A/103, 10-719 Olsztyn, Poland.

  • Submitted10-04-2019|

  • Accepted10-08-2019|

  • First Online 04-10-2019|

  • doi 10.18805/LR-491

Cite article:- Lahuta L.B., Rybiñski W., Bocianowski J., Nowosad K., Börner A. (2019). Raffinose family oligosaccharides in seeds of common vetch (Vicia sativa L. ssp. sativa). Legume Research. 43(4): 512-517. doi: 10.18805/LR-491.

ABSTRACT

The composition and content of low-molecular weight soluble carbohydrates in seeds of 66 accessions of common vetch (Vicia sativa L. ssp. sativa) stored in the Polish and German gene banks, originating from Europe, Asia, Middle East and North Africa, were analyzed using a gas chromatography method. Genotypes showed differences in the composition of carbohydrates depending on the origin of seeds. Common features of accessions were: i) the predominance of raffinose family oligosaccharides (RFOs) in soluble carbohydrates, and ii) verbascose as the main oligosaccharide in RFOs. Several accessions with lowered RFOs content were identified. They may be suitable for breeding focused on diminishing RFOs, as antinutritional compounds in seeds.

REFERENCES

  1. Bocianowski, J., Szulc, P. and Nowosad, K. (2015). Parallel coordinate plots of maize traits under different magnesium applications. Journal of Integrative Agriculture. 14(3):593-597.
  2. Bock, C., Ray, H. and Georges, F. (2009). Downregulation of galactinol synthase in oilseed Brassica napus leads to significant reduction of antinutritional oligosaccharides. Botany. 87:597-603.
  3. Campos-Vega, R., Loarca-Pina, G. and Oomah, B.D. (2010). Minor components of pulses and their potential impact on human health. Food Research International. 43:461-482.
  4. Dierking, E.C. and Bilyeu, K.D. (2009). Raffinose and stachyose metabolism are not required for efficient soybean seed germination. Journal of Plant Physiology. 166:1329-1335.
  5. Duc, G., Marget, P., Esnault, R., Le Guen, J. and Bastianelli, D. (1999). Genetic variability for feeding value of faba bean seeds (Vicia faba): comparative chemical composition of isogenics involving zero-tannin and zero-vicine genes. Journal of Agricultural Science-Cambridge. 133:185-196.
  6. Ford, R., Maddeppungeng, A.M. and Taylor, P.W.J. (2008). Vetch. In: Kole C, Hall TC (eds.) Compendium of Transgenic Crop Plants: Transgenic Legume Seeds and Forages, Vol 9 Blackwell Publishing, Oxford, pp. 163-170.
  7. Frias, J., Bakhash, A., Jones, D.A., Arthur, A.E., Vidal-Valverde, C., Rhodes, M.J.C. and Hedley, C.L. (1999). Genetic analysis of the raffinose oligosaccharide pathway in lentil seeds. Journal of Experimental Botany. 50:469-476.
  8. Gangola, M.P., Khedikar, Y.P., Gaur, P.M., Båga, M. and Chibbar, R.N. (2013). Genotype and growing environment interaction shows a positive correlation between substrates of raffinose family oligosaccharides (RFO) biosynthesis and their accumulation in chickpea (Cicer arietinum L.) seeds. Journal of Agricultural and Food Chemistry. 61:4943-4952.
  9. Gaw³owska, M., Œwiêcicki, W., Lahuta, L. and Kaczmarek, Z. (2017). Raffinose family oligosaccharides in seeds of Pisum wild taxa, type lines for seed genes, domesticated and advanced breeding materials. Genetic Resources and Crop Evolution. 64:569-578.
  10. Hejdysz, M., Kaczmarek, S.A. and Rutkowski, A. (2016). Extrusion cooking improves the metabolisable energy of faba beans and the amino acid digestibility in broilers. Animal Feed Science and Technology. 212:100-111.
  11. Hou, A., Chen, P., Alloatti, J., Li, D., Mozzoni, L., Zhang, B. and Shi, A. (2009). Genetic variability of seed sugar content in worldwide soybean germplasm collections. Crop Science. 49:903-912.
  12. Kalyan, R.K. and Ameta, O.P. (2017). Impact of abiotic factors on seasonal incidence of insect pests of soybean. Legume Research. 40(4):762-767.
  13. Lahuta, L.B. and Goszczyñska, J. (2009). Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch (Vicia villosa Roth.) seeds. Acta Societatis Botanicorum Poloniae. 78:203-208.
  14. Lahuta, L.B., £ogin, A., Rejowski, A., Socha, A. and Zalewski, K. (2000). Influence of water deficit on the accumulation of sugars in developing field bean (Vicia faba var. minor) seeds. Seed Science and Technology. 28:93-100.
  15. Lahuta, L.B., Goszczyñska, J., Horbowicz, M., Ho³dyñski, C. and Górecki, R.J. (2010a). Cyclitols affect accumulation of a-d-galactosides in developing Vicia seeds. Acta Physiologiae Plantarum. 32:933-942.
  16. Lahuta, L.B., Goszczyñska, J. and Horbowicz, M. (2010b). Seed a-D-galactosides of selected Vicia species and enzymes involved in their biosynthesis. Acta Biologica Cracoviensia Series Botanica. 52(1):27-35.
  17. Lahuta, L.B., Ciak, M., Rybiñski, W., Bocianowski, J. and Börner, A. (2018). Diversity of the composition and content of soluble carbohydrates in seeds of the genus Vicia (Leguminosae). Genetic Resources and Crop Evolution. 65(2):541-554.
  18. Lekshmanan, D.K. and Vahab, M.A. (2018). Correlation and path coefficient analysis of yield and its component characters among different accessions of cluster bean [Cyamopsis tetragonoloba (L.) Taub.]. Legume Research. 41:53-56.
  19. Mahalanobis, P.C. (1936). On the generalized distance in statistics. Proceedings of National Institute of Sciences of India. 12:49-55.
  20. Martínez-Villaluenga, C., Frias, J. and Vidal-Valverde, C. (2008). Alpha-galactosides: Antinutritional factors or functional ingredients? Critical Reviews in Food Science and Nutrition. 48(4):301-316.
  21. Mozzoni, L., Shi, A. and Chen, P. (2013). Genetic analysis of high sucrose, low raffinose and low stachyose content in V99-5089 soybean seeds. Journal of Crop Improvement. 27(5):606-616.
  22. Polowick, P.L., Baliski, D.S., Bock, C., Ray, H. and Georges, F. (2009). Over-expression of a-galactosidase in pea seeds to reduce raffinose oligosaccharide content. Botany. 87:526-532.
  23. Seidler-£o¿ykowska, K. and Bocianowski, J. (2012). Evaluation of variability of morphological traits of selected caraway (Carum carvi L.) genotypes. Industrial Crops and Products. 35:140-145.
  24. Sharma, P. and Gaur, N. (2019). Detrimental effect and GC-MS analysis of some plant oils against Polyphagous pests Spodoptera litura and Spilarctia obliqua. Legume Research. 42:392-398.
  25. Szczeciñski, P., Gryff-Keller, A., Horbowicz, M. and Lahuta, L.B. (2000). Galactosylpinitols isolated from vetch (Vicia villosa Roth.) seeds. Journal of Agricultural and Food Chemistry. 48:2717-2720.
  26. Tahir, M., Båga, M., Vandenberg, A. and Chibbar, R.N. (2012). An assessment of raffinose family oligosaccharides and sucrose concentration in genus Lens. Crop Science. 52:1713-1720.
  27. Tahir, M., Vandenberg, A. and Chibbar, R.N. (2011). Influence of environment on seed soluble carbohydrates in selected lentil cultivars. Journal of Food Composition and Analysis. 24:596-602.
  28. Van den Ende, W. (2013). Multifunctional fructans and raffinose family oligosaccharides. Frontiers in Plant Science. 4:247. 
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    Research Article

    volume 43 issue 4 (august 2020) : 512-517,   Doi: 10.18805/LR-491

    Raffinose family oligosaccharides in seeds of common vetch (Vicia sativa L. ssp. sativa)

    L
    L.B. Lahuta
    W
    W. Rybiñski
    J
    J. Bocianowski
    K
    K. Nowosad
    A
    A. Börner
    1Department of Plant Physiology, University of Warmia and Mazury in Olsztyn, Genetics and Biotechnology, Oczapowskiego 1A/103, 10-719 Olsztyn, Poland.

    • Submitted10-04-2019|

    • Accepted10-08-2019|

    • First Online 04-10-2019|

    • doi 10.18805/LR-491

    Cite article:- Lahuta L.B., Rybiñski W., Bocianowski J., Nowosad K., Börner A. (2019). Raffinose family oligosaccharides in seeds of common vetch (Vicia sativa L. ssp. sativa). Legume Research. 43(4): 512-517. doi: 10.18805/LR-491.

    ABSTRACT

    The composition and content of low-molecular weight soluble carbohydrates in seeds of 66 accessions of common vetch (Vicia sativa L. ssp. sativa) stored in the Polish and German gene banks, originating from Europe, Asia, Middle East and North Africa, were analyzed using a gas chromatography method. Genotypes showed differences in the composition of carbohydrates depending on the origin of seeds. Common features of accessions were: i) the predominance of raffinose family oligosaccharides (RFOs) in soluble carbohydrates, and ii) verbascose as the main oligosaccharide in RFOs. Several accessions with lowered RFOs content were identified. They may be suitable for breeding focused on diminishing RFOs, as antinutritional compounds in seeds.

    REFERENCES

    1. Bocianowski, J., Szulc, P. and Nowosad, K. (2015). Parallel coordinate plots of maize traits under different magnesium applications. Journal of Integrative Agriculture. 14(3):593-597.
    2. Bock, C., Ray, H. and Georges, F. (2009). Downregulation of galactinol synthase in oilseed Brassica napus leads to significant reduction of antinutritional oligosaccharides. Botany. 87:597-603.
    3. Campos-Vega, R., Loarca-Pina, G. and Oomah, B.D. (2010). Minor components of pulses and their potential impact on human health. Food Research International. 43:461-482.
    4. Dierking, E.C. and Bilyeu, K.D. (2009). Raffinose and stachyose metabolism are not required for efficient soybean seed germination. Journal of Plant Physiology. 166:1329-1335.
    5. Duc, G., Marget, P., Esnault, R., Le Guen, J. and Bastianelli, D. (1999). Genetic variability for feeding value of faba bean seeds (Vicia faba): comparative chemical composition of isogenics involving zero-tannin and zero-vicine genes. Journal of Agricultural Science-Cambridge. 133:185-196.
    6. Ford, R., Maddeppungeng, A.M. and Taylor, P.W.J. (2008). Vetch. In: Kole C, Hall TC (eds.) Compendium of Transgenic Crop Plants: Transgenic Legume Seeds and Forages, Vol 9 Blackwell Publishing, Oxford, pp. 163-170.
    7. Frias, J., Bakhash, A., Jones, D.A., Arthur, A.E., Vidal-Valverde, C., Rhodes, M.J.C. and Hedley, C.L. (1999). Genetic analysis of the raffinose oligosaccharide pathway in lentil seeds. Journal of Experimental Botany. 50:469-476.
    8. Gangola, M.P., Khedikar, Y.P., Gaur, P.M., Båga, M. and Chibbar, R.N. (2013). Genotype and growing environment interaction shows a positive correlation between substrates of raffinose family oligosaccharides (RFO) biosynthesis and their accumulation in chickpea (Cicer arietinum L.) seeds. Journal of Agricultural and Food Chemistry. 61:4943-4952.
    9. Gaw³owska, M., Œwiêcicki, W., Lahuta, L. and Kaczmarek, Z. (2017). Raffinose family oligosaccharides in seeds of Pisum wild taxa, type lines for seed genes, domesticated and advanced breeding materials. Genetic Resources and Crop Evolution. 64:569-578.
    10. Hejdysz, M., Kaczmarek, S.A. and Rutkowski, A. (2016). Extrusion cooking improves the metabolisable energy of faba beans and the amino acid digestibility in broilers. Animal Feed Science and Technology. 212:100-111.
    11. Hou, A., Chen, P., Alloatti, J., Li, D., Mozzoni, L., Zhang, B. and Shi, A. (2009). Genetic variability of seed sugar content in worldwide soybean germplasm collections. Crop Science. 49:903-912.
    12. Kalyan, R.K. and Ameta, O.P. (2017). Impact of abiotic factors on seasonal incidence of insect pests of soybean. Legume Research. 40(4):762-767.
    13. Lahuta, L.B. and Goszczyñska, J. (2009). Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch (Vicia villosa Roth.) seeds. Acta Societatis Botanicorum Poloniae. 78:203-208.
    14. Lahuta, L.B., £ogin, A., Rejowski, A., Socha, A. and Zalewski, K. (2000). Influence of water deficit on the accumulation of sugars in developing field bean (Vicia faba var. minor) seeds. Seed Science and Technology. 28:93-100.
    15. Lahuta, L.B., Goszczyñska, J., Horbowicz, M., Ho³dyñski, C. and Górecki, R.J. (2010a). Cyclitols affect accumulation of a-d-galactosides in developing Vicia seeds. Acta Physiologiae Plantarum. 32:933-942.
    16. Lahuta, L.B., Goszczyñska, J. and Horbowicz, M. (2010b). Seed a-D-galactosides of selected Vicia species and enzymes involved in their biosynthesis. Acta Biologica Cracoviensia Series Botanica. 52(1):27-35.
    17. Lahuta, L.B., Ciak, M., Rybiñski, W., Bocianowski, J. and Börner, A. (2018). Diversity of the composition and content of soluble carbohydrates in seeds of the genus Vicia (Leguminosae). Genetic Resources and Crop Evolution. 65(2):541-554.
    18. Lekshmanan, D.K. and Vahab, M.A. (2018). Correlation and path coefficient analysis of yield and its component characters among different accessions of cluster bean [Cyamopsis tetragonoloba (L.) Taub.]. Legume Research. 41:53-56.
    19. Mahalanobis, P.C. (1936). On the generalized distance in statistics. Proceedings of National Institute of Sciences of India. 12:49-55.
    20. Martínez-Villaluenga, C., Frias, J. and Vidal-Valverde, C. (2008). Alpha-galactosides: Antinutritional factors or functional ingredients? Critical Reviews in Food Science and Nutrition. 48(4):301-316.
    21. Mozzoni, L., Shi, A. and Chen, P. (2013). Genetic analysis of high sucrose, low raffinose and low stachyose content in V99-5089 soybean seeds. Journal of Crop Improvement. 27(5):606-616.
    22. Polowick, P.L., Baliski, D.S., Bock, C., Ray, H. and Georges, F. (2009). Over-expression of a-galactosidase in pea seeds to reduce raffinose oligosaccharide content. Botany. 87:526-532.
    23. Seidler-£o¿ykowska, K. and Bocianowski, J. (2012). Evaluation of variability of morphological traits of selected caraway (Carum carvi L.) genotypes. Industrial Crops and Products. 35:140-145.
    24. Sharma, P. and Gaur, N. (2019). Detrimental effect and GC-MS analysis of some plant oils against Polyphagous pests Spodoptera litura and Spilarctia obliqua. Legume Research. 42:392-398.
    25. Szczeciñski, P., Gryff-Keller, A., Horbowicz, M. and Lahuta, L.B. (2000). Galactosylpinitols isolated from vetch (Vicia villosa Roth.) seeds. Journal of Agricultural and Food Chemistry. 48:2717-2720.
    26. Tahir, M., Båga, M., Vandenberg, A. and Chibbar, R.N. (2012). An assessment of raffinose family oligosaccharides and sucrose concentration in genus Lens. Crop Science. 52:1713-1720.
    27. Tahir, M., Vandenberg, A. and Chibbar, R.N. (2011). Influence of environment on seed soluble carbohydrates in selected lentil cultivars. Journal of Food Composition and Analysis. 24:596-602.
    28. Van den Ende, W. (2013). Multifunctional fructans and raffinose family oligosaccharides. Frontiers in Plant Science. 4:247. 
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