Priming seed mitigates the effects of saline stress in soybean seedlings

DOI: 10.18805/LR-469    | Article Id: LR-469 | Page : 263-267
Citation :- Priming seed mitigates the effects of saline stress in soybean seedlings.Legume Research.2020.(43):263-267
Z. Miladinov, I. Maksimovic, S. Baleseviæ-Tubic, V. Djukic, P. Canak, J. Miladinovic and V. Djordjevic zlatica.miladinov@ifvcns.ns.ac.rs
Address : Department for Soybean, Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia.
Submitted Date : 6-12-2018
Accepted Date : 17-04-2019


Seed germination and seedling growth are the most sensitive plant stages towards salinity. Various techniques can improve emergence and stand formation under salt conditions. Seeds priming is one of the most frequently used technique. Seed priming could develop different defence mechanisms of seeds against salinity stress. This experiment aims to examine the seed priming into potassium nitrate - KNO3 (1%), ascorbic acid – ASA (100mgl-1), and potassium chloride - KCl (1%) solutions, that may reduce harmful effects of salinity stress. Salinity was imposed by treatment of seeds with different concentrations of NaCl (0 (control), 50, 100, 200m MNaCl). All data were analysed statistically by three-way ANOVA. Individual testing of probability was carried out using Tukey’s method p £ 0.05 and p £ 0.01. Correlation dependence was determined using the Pearson correlation coefficient. Test results demonstrated positive effect of seed priming. This was showed by improved soybean seed quality and increased seed germination rate. The intensity of lipid peroxidation, free proline concentration and Na+ was decreased, while K+ in seedlings increased due to priming treatment. Priming of seeds into ASA solution resulted in increased vitamin C concentration, while priming into KNO3 and ÊCl solutions reduced the content of vitamin Ñ in soybean seedlings. 


Germination Priming Salinity Soybean.


  1. Arun M. N., Shankara Hebbar S., Bhanuprakas K., Senthivel T. (2017). Seed priming improves irrigation water use efficiency, yield and yield components of summer cowpea under limited water conditions. Legume Research, 40(5): 864–871.
  2. Bates L. S., Waklren R. P., Teare I. D. (1973). Rapid determination of free proline water stress studies. Plant and Soil, 39: 205–207.
  3. Benderitter M., Maupoli V., Vergely C., Dalloz F., Briot F., Rochette L. (1998). Studies by electron paramagnetic resonance of the importance of iron in the hydroxyl scavenging properties of ascorbic acid in plasma: Effects of iron chelators. Fundamental and Clinical Pharmacology, 12 (9): 510–516.
  4. ISTA (International seed testing association) (2008). International Rules for Seed Testing. ISTA, Zurich. https://www.seedtest.org/en/    home.html
  5. Li R., Min D., Chen L., Chen C., Hu X. (2017). Hydropriming accelerates seed germination of Medicago sativa under stressful conditions: A thermal and hydrotime model approach. Legume Research, 40(3): 401–408.
  6. Miladinov Z., Baleševiæ-Tubiæ S., Ðorðeviæ V., Ðukiæ V., Iliæ A., Èobanoviæ L. (2015). Optimal time of soybean seed priming and primer effect under salt stress conditions. Journal of Agricultural Sciences, 60(2): 109–117. 
  7. Noctor G., Foyer C. H. (1998). Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 49: 249–279.
  8. Placer Z. A., Cushman L. L., Johnson B. C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry, 16(2): 359–364.
  9. Sadeghipour O. (2017). Amelioration of salinity tolerance in cowpea plants by seed treatment with methyl jasmonate. Legume Research, 40(6): 1100–1106.
  10. Viradiya M. B., Golakiya B. A., Kikani V. L. (2008). Effect of potassium and salinity levels on nutritient concentration of ground nut (Arachis hypogea L.). Legume Research, 31(3):196–198. 

Global Footprints