Integrated nutrient management in cowpea with the application of microbial inoculants

DOI: 10.18805/LR-4102    | Article Id: LR-4102 | Page : 243-251
Citation :- Integrated nutrient management in cowpea with the application of microbial inoculants.Legume Research.2021.(44):243-251
S. Ramya and Gulab Pandove gpandove@pau.edu
Address : Department of Microbiology, Punjab Agricultural University, Ludhiana-141 004, Punjab, India.
Submitted Date : 28-11-2018
Accepted Date : 20-02-2019


Indian livestock sector is one of the largest in the world and fodder is vital component of profitable animal production. Legumes are one of the predominant crops of mixed crop-livestock systems providing highly nutritious fodder as well as contributing soil fertility. Nevertheless, the fodder production and quality in the country is not adequate to meet out the prerequisite of growing livestock population. Optimum nutrition is thus required for getting the maximum forage yield and quality. Nutrient Management is propounded as a promising strategy for addressing such challenges. Microbial inoculants being an important component of integrated nutrient management are eco-friendly and economical sources of nutrient. The benefit of combining organic and inorganic sources of nutrients in integrated nutrient management has proved superior to the use of each component individually. Thus, the present review will feature the need of integrated nutrient management, plant growth promoting rhizobacteria as microbial inoculants, role of microbial inoculants in integrated nutrient management of various leguminous crops and emerging examples of integrated nutrient management in cowpea. The realization attained from literature assessed herein will further help to understand the role of microbial inoculants in integrated nutrient management.  


Cowpea Fodder Livestock Microbial inoculants Integrated nutrient management.


  1. Afzal A and Bano A. (2008). Rhizobiumand phosphate solubilizing bacteria improve the yieldand phosphorus uptake in wheat (Triticum aestivum). International Journal of Agriculture and biology, 10: 85-88.
  2. Ahmed M and Kibret M. (2014). Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University – Science, 26: 1-20.
  3. Ahmad A, Tomar GS, Taunk SK and Verma N. (2017). Evaluation of new cultivars of fodder cowpea in terms of growth attributes and seed yield as influenced by various doses and varieties Int.J.Curr.Microbiol.App.Sci, 6(10): 937-944.
  4. Akibode CS. (2011). Trends in the production, trade and consumption of food-legume crops in sub-Saharan Africa. Master of Science Thesis in Agricultural Food, and Resource Economics, Michigan State University. 
  5. Ali B, Sabri AN and Hasnain S. (2010). Rhizobacterial potential to alter auxin content and growth of Vigna radiata (L.). World Journal of Microbiology and Biotechnology, 26: 1379–84.
  6. Antil RS (2012). Integrated Plant Nutrient Supply for Sustainable Soil Health and Crop Productivity, A.Kumar (ed.) Vol. 3. Focus Global Reporter.
  7. Anonymous. (2011). IGFRI Vision 2030. Indian Grassland and Fodder Research Institute, Jhansi (U. P.), India.
  8. Anonymous (2018). Package of Practices for Crops of Punjab-Kharif. Punjab Agricultural University, Ludhiana.
  9. Ashwathy AJ, Jasim B, Jyothis M and Radhakrishnan EK. (2012). Identification of two strains of Paenibacillus sp. as indole 3 acetic acid-producing rhizome-associated endophytic bacteria from curcuma longa. Biotech, 3:219-224.
  10. Ayoola OT and Makinde EA. (2007). Fertilizer treatment effects on performance of cassava under two planting patterns in a cassava based cropping system in south west Nigeria. Research Journal of Agriculture and Biological Science, 3: 13-20. 
  11. Band AM, Mendhe SN, Kolte HS, Choudhary RL, Verma R and Sharma SK. (2007). Nutrient management studies in French bean (Phaseolus vulgaris L.). Journal of Soils and Crops 17: 367-372.
  12. Bashan Y, Salazar B, Puente ME. (2009). Responses of native legume desert trees used for reforestation in the Sonoran Desert to plant growth-promoting microorganisms in screen house. Biol Fertile Soils, 45:655–662.
  13. Borasate A. (2009). Optimization of growth and production of protease by Penicillium species using submerged fermentation.” International Journal of Microbiology Research .Pp14-18. 
  14. Bedmar EJ, Robles EF and Delgado MJ. (2005). The complete denitrification pathway of the symbiotic, nitrogen-fixing bacterium Bradyrhizobium japonicum. Biochem. Soc. Trans. 33: 141–144.
  15. Behera UK, Pradhan S and Sharma AR. (2007). Effect of integrated nutrient management practices on productivity of durum wheat (Triticum durum) in the Vertisols of central India. Annals of Plant and Soil Research, 9: 21-24.
  16. Bhardwaj SK, Kaushal R, Sharma Y and Chaudhary V. (2010). Effect of conjoint use of inorganic fertilizers and organics on soil fertility and growth parameters of tomato and French bean crops in mid hills of Himachal Pradesh. Progressive Horticulture, 42: 58-64.
  17. Cassman KG, Dobermann A, and Walters DT. (2002). Agroecosystems, nitrogen use efficiency, and nitrogen management. Ambio, 31: 132–140.
  18. Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA and Young CC. (2006). Phosphate solubilizing bacteria from subtropical soil and their tri calcium phosphate solubilizing abilities. Applied Soil Ecology, 34: 33-41. 
  19. Choudhary SK, Jat MK, Sharma SR and Singh P. (2011). Effect of INM on soil nutrient and yield in groundnut field of semi-arid area of Rajasthan. Legume Res., 34: 283-287. 
  20. Das B, Wagh AP, Dod VN, Nagre PK and Bawkar SO. (2011). Effect of integrated nutrient management on cowpea. The Asian Journal of Horticulture, 6: 402-405.
  21. Das AJ, Kumar M and Kumar R. (2013). Plant Growth Promoting Rhizobacteria (PGPR): An Alternative of Chemical Fertilizer for Sustainable, Environment Friendly Agriculture. Research Journal of Agriculture and Forestry Sciences, 1: 21-23. 
  22. Dhavappriya A, Sanjivkumar V and Kumaran ST. (2015). Studies on the impact and efficiency of integrated nutrient management on yield, major and secondary nutrient content of okra crop for sustainable agriculture. International Journal of agricultural Science, 11(1): 63-67.
  23. El-Shaikh, KAA, EI-Dakkak AAA and Obiadalla- Ali HA. (2010). Maximizing productivity of some garden pea cultivars and minimizing chemical phosphorus fertilizer via VA mycorrhizal inoculants. Journal of Horticultural Science & Ornamental Plants, 2: 114-122.
  24. Esilaba AO, Byalebeka JB, Delve RJ, Okalebo JR, Senyange D, Balule M and Sali H. (2004). On farm testing of integrated nutrient management strategies in eastern Uganda. Agricultural System, 86: 144–165.
  25. Fatokun C, Boukar O, Muranaka S and Chikoye D. (2009). Enhancing drought tolerance in cowpea. African Crop Science Conference Proceedings. 9: 531-536.
  26. Fraile PG, Menéndez E and Rivas R. (2015). Role of bacterial biofertilizers in agriculture and forestry. Bioeng, 2(3): 183-205.
  27. Giller KE. (2001). Nitrogen Fixation in Tropical Cropping Systems. Wallingford, CT: CAB International. 
  28. Gruhn P, Golleti F and Yudelman M. (2000). Integrated Nutrient Management, Soil Fertility and Sustainable Agriculture: Current Issues and Future Challenges. Washington D.C. International Food Policy Research Institute. Food, Agriculture and Environment    Discussion Paper 32.
  29. Hadad EI, ME, Mustafa MI, Selim SM, Mahgoob AEA and EI-Tayeb TS. (2010). In vitro evaluation of some bacterial isolates from Ethiopia and South Africa. Journal of Biological Control, 45: 72-84.
  30. Hari M and Perumal K (2010). Booklet on Bio-fertilizer(phosphabacteria). Shri Annm Murugapa Chettiar Research Centre Taramani Chennai, pp.1–6.
  31. Hayat R, Ali S, Amara U, Khalid R and Ahmed I. (2010). Soil beneficial bacteria and their role in plant Plant Growth Promotion: A Review. Annals of Microbiology, 60: 579-598. 
  32. https://ncof.dacnet.nic.in/OrganicInputproductionstatistics/BFandOFProductionstatistics2010-11to2011-12.pdf
  33. IGFRI Vision. 2050. Indian Grassland and Fodder Research Institute, Jhansi (UP) – 284003.
  34. Jeffries P, Gianinazzi S, Perotto S, Turnau K and Barea JM. (2003). The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biological Fertilizers Soils, 37: 1–16.
  35. Jha A, Sharma D and Saxena J. (2012). Effect of single and dual phosphate solubilizing bacteria strain inoculations on overall growth of mungbean plants. Archives of Agronomy and Soil Science, 58: 967-981.
  36. John RP, Tyagi RD, Brar SK, Prevost D. (2010). Development of emulsion from rhizobial fermented starch industry wastewater for application as Medicago sativa seed coat. Eng Life Sci, 10:248–25.
  37. Kahlon CS and Sharanappa RK. (2006). Nutrient uptake, quality and balance of nutrients as influenced by phosphorus, bio-inoculants, zinc and sulphur in cowpea [Vigna unguiculata (L.) Walp]. Environment and Ecology, 245: 220-233.
  38. Kannan RL, Dhivya M, Abinaya D, Krishna RL and Krishna kumar S. (2013). Effect of Integrated Nutrient Management on Soil Fertility and Productivity in Maize. Bulletin of Environment, Pharmacology and Life Sciences, 2: 61-67.
  39. Kannan V and Sureendar R. (2009). Synergistic effect of beneficial rhizosphere microflora in biocontrol and plant growth promotion. Journal of Basic Microbiology, 49: 158-164.
  40. Kormata P, Tamo M, Fatokum C, Taraali C and Singh B. (2000). Challenges and opportunities for enhancing sustainable cowpea production. Proceedings of the World Cowpea Conference III held in International Institute of Tropical Agriculture (IITA).    Ibadan,Nigeria.
  41. Khosro M and Yousef S. (2012). Bacterial bio-fertilizers for sustainable crop production: A review APRN Journal of Agricultural and Biological Science, 7: 237-308.
  42. Kloepper JW, Leong J, Teintze M and Schroth MN. (1980). Enhancing plant growth by siderophores produced by plant growth-    promoting rhizobacteria. Nature, 286: 885–886.
  43. Kumar MK, Patel IC and Ali S. (2012). Integrated nutrient management in clusterbean (Cyamopsis tetragonoloba L.). Legume Research, 35: 350-353.
  44. Kumar A and Pandita VK (2016). Effect of integrated nutrient management on seed yield and quality in cowpea. Legume Research, 39: 448-452.
  45. Kyei-Boahen S, Canon EN, Chikoye D and Abaidoo R (2017). Growth and Yield Responses of Cowpea to Inoculation and Phosphorus Fertilization in Different Environment. Front. Plant Sci. 8:646.
  46. Lingaraju NN, Hunshal CS and Salakinkop SR. (2016). Effect of biofertilizers and foliar application of organic acids on yield, nutrient uptake and soil microbial activity in soybean. Legume Research, 39(2): 256-261. 
  47. Makoi JHJR, Bambara S and Ndakidemi PA. (2013). “Rhizobium Inoculation and the Supply of Molybdenum and Lime Affect the Uptake of Macroelements in Common Bean (P. vulgaris L.) Plants.” Aust. J. Crop Sci. 7: 784-794.
  48. Mandal MK, Pati R, Mukhopadhyaya, D and Majumdar K. (2009). Maximizing yield of cowpea through soil test based nutrient application in Tarai alluvial soils. Better Crop India 28-30. 
  49. Mathew MM and Hameed SMS. (2002). Influence of microbial inoculants and phosphorus levels on the root characters, growth and yield of vegetable cowpea [Vigna unguiculata subsp. sesquipedalis (L.) Verdcourt]. Journal of Tropical Agriculture, 40: 71-73.
  50. Marimuthu S, Surendranb U and Subbianc P. (2014). Productivity, nutrient uptake and post-harvest soil fertility as influenced by cotton-based cropping system with integrated nutrient management practices in semi-arid tropics. Archives of Agronomy and Soil Science, 60(1): 87-101.
  51. Martinez-Romero and Wang ET. (2000). Sesbania herbacea-Rhizobium huautlense nodulation in flooded soils and comparative characterization of S. herbacea-nodulating rhizobia in different environments. Microbiology Ecology, 40: 25–32.
  52. Meena LR and Mann JS. (2006). Strategic nutrient supplementation in berseem for higher biomass productivity and economic return under semiarid conditions. Range Management and Agroforestry, 27: 40-43.
  53. Ministry of agriculture (MOA) (2012). Economic review and planning paper.
  54. Nath DJ, Ozah B, Baruah R, Barooah RC and Borah DK. (2011). Effect of integrated nutrient management on soil enzymes, microbial biomass carbon and bacterial populations under rice (Oryza sativa)–wheat (Triticum aestivum) sequence. Indian Journal of Agricultural Science, 81: 1143–1148.
  55. Negi S, Singh RV and Dwivedi DK. (2006). Effect of biofertilizers, nutrient sources and lime on growth and yield of garden pea. Legume Research, 29: 282-285.
  56. Palaniappan SP and Annadurai K. (2007). Organic farming: theory and practices, Scientific Publishers, Jodhpur, Rajasthan (India). 
  57. Patel D, Arvadia MK and Patel AJ (2007), Effect of integrated nutrient management on growth, yield and nutrient uptake by chickpea on vertisol of south Gujarat. Journal of Food Legume, 20: 113-114.
  58. Patel PS, Patel IS, Panickar B and Ravindrababu Y. (2012). Management of sucking pests of cowpea through seed treatment. Trends in Biosciences, 5: 138-139.
  59. Prasad M, Dawson J, and Yadav RS. (2012). Effect of different nitrogen sources and phosphate solubilizing bacteria on growth and yield of grain cowpea [Vigna unguiculata (L.) Walp.]. Crop Research, 44: 59-62.
  60. Pramanik K and Bera AK. (2012). Response of biofertilizer and phytohormone on growth and yield of chick pea (Cicer arietinum L.). Journal of Crop and Weed, 8: 45-49.
  61. Ramana V, Ramakrishna M, Purushotham K and Reddy KB (2010). Effect of bio-fertilizers on growth, yield attributes and yield of french bean (Phaseolus vulgaris L.). Legume Research, 33: 178–183.
  62. Reichman SM. (2007). The Potential Use of the Legume-Rhizobium Symbiosis for the Remediation of Arsenic Contaminated Sites. Soil Biology & Biochemistry, 39:2587-2593. 
  63. Sayyed R and Chincholkar S. (2009). Siderophore-Producing Alcaligenes feacalis Exhibited More Biocontrol Potential Vis-à-Vis Chemical Fungicide. Current Microbiology, 58: 47-51. 
  64. Senthilkumar PK and Sivagurunathan P. (2012). Comparative effect on bacterial biofertilizers on growth and yield of green gram (Phaseolus radiata L.) and cow pea (Vigna siensis Edhl.). International. Journey of Current Microbiology Applied Science, 1: 34-39.
  65. Singh DK, Chand L, Singh KN and Singh JK. (2006). Effect of different biofertilizers in combination with chemical fertili zers on pea (Pisum sativum L.) under temperate Kashmir conditions. Environment and Ecology, 24: 684-686.
  66. Singh AK, Tripathi PN and Singh R. (2007). Effect of rhizobium Inoculation, nitrogen and phosphorous levels on growth, yield and quality of kharif cowpea. (Vigna unguiculata) (L.) Walp. Crop Research, 33: 71-73.
  67. Singh SR, Bhat MI, Wani JA and Najar GR. (2009). Role of Rhizobium and VAM fungi for improvement i n fertility and yield of greengram under temperate conditions. Journal of the Indian Society of Soil Science, 57: 45-52. 
  68. Stancheva I, Geneva M, Zehirov G, Tsvetkova G, Hristozkova M, Georgiev G. (2006). Effects of combined inoculation of pea plants with arbuscular mycorrhizal fungi and rhizobium on nodule formation and nitrogen fixing activity. Gen. Appl. Plant Physiology 61-66.
  69. Swaroop K and Rathore SVS. (2002). Economics, nutrient content and pod yield of vegetable cowpea in relation to application of P K and Rhizobium biofertilizer in Andaman. Indian Agriculturist, 46: 153-160.
  70. Tank N and Saraf M. (2009). Enhancement of Plant Growth and Decontamination of Nickel-Spiked Soil Using PGPR. Journal of Basic Microbiology, 49: 195-204.
  71. Thamer S, Schadler M, Bonte D and Ballhorn DJ. (2011). Dual benefit from a belowground symbiosis: Nitrogen fixing rhizobia promote growth and defense against a specialist herbivore in a cyanogenic plant. Plant and Soil, 341:209–219.
  72. Trimurtulu N and Rao DLN. (2014). Liquid Microbial Inoculants and their Efficacy on Field Crops, ANGRAU, Agricultural Research Station, Amaravathi, pp 54.
  73. Vedram Mishra, SK and Upadhyay RM. (2002). Effect of Sulphur,Zinc and biofertilizer on quality character in Mung bean. Indian Journal Pulse Research, 15: 139-141.
  74. Vessey JK. (2003). Plant growth promoting rhizobacteria as biofertilizers. Journal of Plant and Soil, 255: 571-586.
  75. Wani PA, Khan S and Zaidi A. (2008). Effect of Metal-Tolerant Plant Growth-Promoting Rhizobium on the Performance of Pea Grown in Metal-Amended Soil. Archives of Environmental Contamination and Toxicology, 55: 33-42.
  76. Zabihi H, Savaghebi G, Khavazi K, Ganjali A and Miransari M. (2011).Pseudomonas bacteria and phosphorous fertilization, affecting wheat (Triticum aestivum L.) yield and P uptake under greenhouse and field conditions. Acta Physiologiae Plantarum, 33: 145–52.
  77. Zahir ZA, Arshad M and Frankenberger WT. (2004). Plant growth promoting rhizobacteria: Applications and perspectives in agriculture. Advances in Agronomy, 81: 97-168.
  78. Zhang F, Cui Z, Chen X, Ju X, Shen J, Chen Q, Liu X, Zhang W, Mi G, Fan M and Jiang R. (2012). Integrated nutrient management for food security and environmental quality in China. Adv Agronomy, 116: 1–40. 

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