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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 41 issue 5 (october 2018) : 778-783,   Doi: 10.18805/LR-408

Antibacterial activity of the seeds, roots and shoots of Lotus populations
 

G
Gurkan Demirkol
1Ordu University, Faculty of Agriculture, Department of Field Crops, Ordu, Turkey.

  • Submitted20-01-2018|

  • Accepted26-04-2018|

  • First Online 16-07-2018|

  • doi 10.18805/LR-408

Cite article:- Demirkol Gurkan (2018). Antibacterial activity of the seeds, roots and shoots of Lotus populations. Legume Research. 41(5): 778-783. doi: 10.18805/LR-408.

ABSTRACT

In this study the antibacterial activity of the ethanol, ethyl acetate and chloroform extracts from the seeds, roots and shoots of Lotus aegaeus, Lotus angustissimus, Lotus corniculatus, Lotus gebelia, Lotus palustris populations grown naturally in Turkey were investigated by using the disc diffusion and agar dilution method, against main plant pathogenic bacteria (Clavibacter michiganensis, Agrobacterium tumefaciens, Erwinia caratovora, Pseudomonas phaseolicola). According to results, the shoot ethyl acetate extract of L. aegaeus and shoot extracts of L. corniculatus against C. michiganensis and shoot extracts of all solvents of L. angustissimus against P. phaseolicola showed high antibacterial activity. This is the first report of antibacterial activity of the Lotus species against plant pathogens. In the study these stated effective extracts showed higher antibacterial effects by comparison with used chemical preservatives against sensitive bacteria. This study offers that active compounds present in Lotus species could play a big role in naturally plant preservation against plant diseases.

REFERENCES

  1. Armand, N., Amiri, H., and Ismaili, A. (2016). The effects of spraying methanol solution and drought stress on level of antioxidant enzyme activity, growth, biomass and yield of beans (Phaseolus vulgaris L. cv. COS16). Legume Research: An International Journal, 39(3): 385-395.
  2. Badawy, M.E. (2010). Structure and antimicrobial activity relationship of quaternary N alkyl chitosan derivatives against some plant pathogens. Journal of Applied Polymer Science, 117(2): 960-969.
  3. Badawy, M.E. and Rabea, E.I. (2011). A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection. International Journal of Carbohydrate Chemistry. 1-29.
  4. Boulaaba, M., Snoussi, M., Saada, M., Mkadmini, K., Smaoui, A., Abdelly, C., and Ksouri, R. (2015). Antimicrobial activities and phytochemical analysis of Tamarix gallica extracts. Industrial Crops and Products, 76: 1114-1122.
  5. Dalmarco, J.B., Dalmarco, E.M., Koelzer, J., Pizzolatti, M.G., and Fröde, T.S. (2010). Isolation and identification of bioactive compounds responsible for the anti-bacterial efficacy of Lotus corniculatus var. São Gabriel. International Journal of Green Pharmacy, 4(2): 108-114.
  6. Davis, P.H. (1966-1988). Flora of Turkey and the East Aegean Islands, Vols. 1-10. Edinburgh University Press, Edinburgh.
  7. Frame, J. (2005). Forage legumes for temperate grasslands. Rome: FAO, Enfield, NH: Science Publishers Inc. pp 84-90.
  8. Girardi, F.A., Tonial, F., Chini, S.O., Sobottka, A.M., Scheffer-Basso, S.M., and Bertol, C.D. (2014). Phytochemical profile and antimicrobial properties of Lotus spp.(Fabaceae). Anais da Academia Brasileira de Ciências, 86(3), 1295-1302.
  9. Hajlaoui, H., Snoussi, M., Ben Jannet, H., Mighri, Z., and Bakhrouf, A. (2008). Comparison of chemical composition and antimicrobial activities of Mentha longifolia L. ssp. longifolia essential oil from two Tunisian localities (Gabes and Sidi Bouzid). Annals of Microbiology, 58: 513-520.
  10. Haritha, P. and Maheswari, K.U. (2007). Effect of processing on the antinutritional and antimicrobial activity of sicklesenna seeds (Cassia tora L.). Legume Research-An International Journal, 30(2): 108-112.
  11. Hemphill, L. and Cobiac, L. (2006). Health benefits of herbs and spices: the past, the present, the future. The Medical Journal of Australia, 185: 5-6.
  12. Merkouropoulos, G., Hilioti, Z., Abraham, E.M., Lazaridou, M. (2017). Evaluation of Lotus corniculatus L. accessions from different locations at different altitudes reveals phenotypic and genetic diversity. Grass and Forage Science, 72(4): 851-856.
  13. Mulaudzi, R.B., Ndhlala, A.R., Kulkarni, M.G., Finnie, J.F., and Van Staden, J. (2011). Antimicrobial properties and phenolic contents of medicinal plants used by the Venda people for conditions related to venereal diseases. Journal of Ethnopharmacology, 135: 330-337.
  14. Muzzarelli, R.A. (1983). Chitin and its derivatives: new trends of applied research. Carbohydrate Polymers, 3(1): 53-75.
  15. Negi, P.S. (2012). Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. International Journal of Food Microbiology, 156(1): 7-17.
  16. Patil, S.L., Mishra, P.K., Loganandhan, N., Math, S.K.N., Manikatti, S.M., Seshadri, B.N. (2015). Suitable chickpea cultivars for rainfed situations in black soils of South India. Legume Research-An International Journal, 38(2): 229-234.
  17. Reynaud, J. and Lussignol, M. (2005). The flavonoids of Lotus corniculatus. Lotus Newsletter, 35(1): 75-82.
  18. Robbins, M.P., Paolocci, F., Hughes, J.W., Turchetti, V., Allison, G., Arcioni, S., Morris, P., Damiani, F. (2003). Sn, a maize bHLH gene, modulates anthocyanin and condensed tannin pathways in Lotus corniculatus. Journal of Experimental Botany, 54(381): 239-248.
  19. Rochon, J.J., Doyle, C.J., Greef, J.M., Hopkins, A., Molle, G., Sitzia, M., Scholefield, D., Smith, C.J. (2004). Grazing legumes in Europe: a review of their status, management, benefits, research needs and future prospects. Grass and Forage Science, 59(3): 197-214.
  20. Vander Berghe, D.A. and Vietinck, A.J. (1991). Screening methods for antibacterial and antiviral agents from higher plants. In Methods in Plant Biochemistry (P.M. Dey and J.B. Harborne, eds.) Academic Press, London, 47-67.
  21. Villa-Ruano, N., Pacheco-Hernández, Y., Cruz-Durán, R., and Lozoya-Gloria, E. (2015). Volatiles and seasonal variation of the essential oil composition from the leaves of Clinopodium macrostemum var. laevigatum and its biological activities. Industrial Crops and Products, 77: 741-747.
  22. Wang, Y., Douglas, G.B., Waghorn, G.C., Barry, T.N., Foote, A.G. (1996). Effect of condensed tannins in Lotus corniculatus upon lactation performance in ewes. The Journal of Agricultural Science, 126(3): 353-362. 
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    Research Article

    volume 41 issue 5 (october 2018) : 778-783,   Doi: 10.18805/LR-408

    Antibacterial activity of the seeds, roots and shoots of Lotus populations
     

    G
    Gurkan Demirkol
    1Ordu University, Faculty of Agriculture, Department of Field Crops, Ordu, Turkey.

    • Submitted20-01-2018|

    • Accepted26-04-2018|

    • First Online 16-07-2018|

    • doi 10.18805/LR-408

    Cite article:- Demirkol Gurkan (2018). Antibacterial activity of the seeds, roots and shoots of Lotus populations. Legume Research. 41(5): 778-783. doi: 10.18805/LR-408.

    ABSTRACT

    In this study the antibacterial activity of the ethanol, ethyl acetate and chloroform extracts from the seeds, roots and shoots of Lotus aegaeus, Lotus angustissimus, Lotus corniculatus, Lotus gebelia, Lotus palustris populations grown naturally in Turkey were investigated by using the disc diffusion and agar dilution method, against main plant pathogenic bacteria (Clavibacter michiganensis, Agrobacterium tumefaciens, Erwinia caratovora, Pseudomonas phaseolicola). According to results, the shoot ethyl acetate extract of L. aegaeus and shoot extracts of L. corniculatus against C. michiganensis and shoot extracts of all solvents of L. angustissimus against P. phaseolicola showed high antibacterial activity. This is the first report of antibacterial activity of the Lotus species against plant pathogens. In the study these stated effective extracts showed higher antibacterial effects by comparison with used chemical preservatives against sensitive bacteria. This study offers that active compounds present in Lotus species could play a big role in naturally plant preservation against plant diseases.

    REFERENCES

    1. Armand, N., Amiri, H., and Ismaili, A. (2016). The effects of spraying methanol solution and drought stress on level of antioxidant enzyme activity, growth, biomass and yield of beans (Phaseolus vulgaris L. cv. COS16). Legume Research: An International Journal, 39(3): 385-395.
    2. Badawy, M.E. (2010). Structure and antimicrobial activity relationship of quaternary N alkyl chitosan derivatives against some plant pathogens. Journal of Applied Polymer Science, 117(2): 960-969.
    3. Badawy, M.E. and Rabea, E.I. (2011). A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection. International Journal of Carbohydrate Chemistry. 1-29.
    4. Boulaaba, M., Snoussi, M., Saada, M., Mkadmini, K., Smaoui, A., Abdelly, C., and Ksouri, R. (2015). Antimicrobial activities and phytochemical analysis of Tamarix gallica extracts. Industrial Crops and Products, 76: 1114-1122.
    5. Dalmarco, J.B., Dalmarco, E.M., Koelzer, J., Pizzolatti, M.G., and Fröde, T.S. (2010). Isolation and identification of bioactive compounds responsible for the anti-bacterial efficacy of Lotus corniculatus var. São Gabriel. International Journal of Green Pharmacy, 4(2): 108-114.
    6. Davis, P.H. (1966-1988). Flora of Turkey and the East Aegean Islands, Vols. 1-10. Edinburgh University Press, Edinburgh.
    7. Frame, J. (2005). Forage legumes for temperate grasslands. Rome: FAO, Enfield, NH: Science Publishers Inc. pp 84-90.
    8. Girardi, F.A., Tonial, F., Chini, S.O., Sobottka, A.M., Scheffer-Basso, S.M., and Bertol, C.D. (2014). Phytochemical profile and antimicrobial properties of Lotus spp.(Fabaceae). Anais da Academia Brasileira de Ciências, 86(3), 1295-1302.
    9. Hajlaoui, H., Snoussi, M., Ben Jannet, H., Mighri, Z., and Bakhrouf, A. (2008). Comparison of chemical composition and antimicrobial activities of Mentha longifolia L. ssp. longifolia essential oil from two Tunisian localities (Gabes and Sidi Bouzid). Annals of Microbiology, 58: 513-520.
    10. Haritha, P. and Maheswari, K.U. (2007). Effect of processing on the antinutritional and antimicrobial activity of sicklesenna seeds (Cassia tora L.). Legume Research-An International Journal, 30(2): 108-112.
    11. Hemphill, L. and Cobiac, L. (2006). Health benefits of herbs and spices: the past, the present, the future. The Medical Journal of Australia, 185: 5-6.
    12. Merkouropoulos, G., Hilioti, Z., Abraham, E.M., Lazaridou, M. (2017). Evaluation of Lotus corniculatus L. accessions from different locations at different altitudes reveals phenotypic and genetic diversity. Grass and Forage Science, 72(4): 851-856.
    13. Mulaudzi, R.B., Ndhlala, A.R., Kulkarni, M.G., Finnie, J.F., and Van Staden, J. (2011). Antimicrobial properties and phenolic contents of medicinal plants used by the Venda people for conditions related to venereal diseases. Journal of Ethnopharmacology, 135: 330-337.
    14. Muzzarelli, R.A. (1983). Chitin and its derivatives: new trends of applied research. Carbohydrate Polymers, 3(1): 53-75.
    15. Negi, P.S. (2012). Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. International Journal of Food Microbiology, 156(1): 7-17.
    16. Patil, S.L., Mishra, P.K., Loganandhan, N., Math, S.K.N., Manikatti, S.M., Seshadri, B.N. (2015). Suitable chickpea cultivars for rainfed situations in black soils of South India. Legume Research-An International Journal, 38(2): 229-234.
    17. Reynaud, J. and Lussignol, M. (2005). The flavonoids of Lotus corniculatus. Lotus Newsletter, 35(1): 75-82.
    18. Robbins, M.P., Paolocci, F., Hughes, J.W., Turchetti, V., Allison, G., Arcioni, S., Morris, P., Damiani, F. (2003). Sn, a maize bHLH gene, modulates anthocyanin and condensed tannin pathways in Lotus corniculatus. Journal of Experimental Botany, 54(381): 239-248.
    19. Rochon, J.J., Doyle, C.J., Greef, J.M., Hopkins, A., Molle, G., Sitzia, M., Scholefield, D., Smith, C.J. (2004). Grazing legumes in Europe: a review of their status, management, benefits, research needs and future prospects. Grass and Forage Science, 59(3): 197-214.
    20. Vander Berghe, D.A. and Vietinck, A.J. (1991). Screening methods for antibacterial and antiviral agents from higher plants. In Methods in Plant Biochemistry (P.M. Dey and J.B. Harborne, eds.) Academic Press, London, 47-67.
    21. Villa-Ruano, N., Pacheco-Hernández, Y., Cruz-Durán, R., and Lozoya-Gloria, E. (2015). Volatiles and seasonal variation of the essential oil composition from the leaves of Clinopodium macrostemum var. laevigatum and its biological activities. Industrial Crops and Products, 77: 741-747.
    22. Wang, Y., Douglas, G.B., Waghorn, G.C., Barry, T.N., Foote, A.G. (1996). Effect of condensed tannins in Lotus corniculatus upon lactation performance in ewes. The Journal of Agricultural Science, 126(3): 353-362. 
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