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Research Article

volume 43 issue 5 (october 2020) : 617-622,   Doi: 10.18805/LR-4029

Diversity analysis by SSR markers and morphological markers among Stemphylium blight tolerance genotypes with high yielding cultivars of Lentil (Lens Culinaris Medik)

D
Debarati Mandal
P
Prabir Kumar Bhattacharyya
R
Raju Das
S
Somnath Bhattacharyya
1Department of Genetics and Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur-741252, Nadia, West Bangal.

  • Submitted18-04-2018|

  • Accepted05-06-2018|

  • First Online 20-08-2018|

  • doi 10.18805/LR-4029

Cite article:- Mandal Debarati, Bhattacharyya Kumar Prabir, Das Raju, Bhattacharyya Somnath (2018). Diversity analysis by SSR markers and morphological markers among Stemphylium blight tolerance genotypes with high yielding cultivars of Lentil (Lens Culinaris Medik). Legume Research. 43(5): 617-622. doi: 10.18805/LR-4029.

ABSTRACT

Twelve Simple Sequence Repeat (SSR) markers along with twelve morphological markers were used to assess the diversity among thirteen screened genotypes both moderate resistant and susceptible against stemphylium blight of indigenous microsperma germplasm (i.e. pilosae) type of Lentil (Lens culinaris Medik.) at BCKV, West Bengal. Polymorphic Information Content (PIC) of SSR primers ranged from 0.77 (SSR 317-2) to 0.15 (PBA LC 0233). The maximum Jaccard’s similarity was recorded between L-4769 and Ranjan (approximately 100%) whereas minimum was between K-75 and VL 126 (0.11). Genotypes K-75 also showed less similarity with DL 14-2 and VL 151 (0.17). Another popular variety WBL 77 showed minimum similarity with moderately resistant genotypes VL 151 and DL 14-2 (0.17). While using morphological characters as the basis of diversity analysis, Euclidean coefficient revealed that high yielding cultivar Subrata was maximum diverse with genotype VL 151(7.12).  Asha also showed diversity with VL 151(6.31) whereas VL 151 had dissimilarity coefficient of 6.21 with K-75. Precoz and VL 126 has dissimilarity coefficient of 6.19. On the basis of above findings it could be said that diverse parental pairs VL 151 & K 75 and VL 151 and Subrata / Asha could be used in hybridization programme or biotic stress breeding programme for developing Stemphylium resistant lentil cultivars in future.

REFERENCES

  1. Abe, J., Xu D. H., Suzuki Y., Kanazawa A. and Shimanmoto Y. (2003). Soybean germplasm pools in Asia revealed by nuclear SSRs. Theor Appl Genet. 106:445–453.
  2. Datta, S., Tiwari S., Kaashyap M., Gupta P. P., Choudhury P. R., Kumari J., Kumar S. (2011). Genetic Similarity Analysis in Lentil Using Cross-Genera Legume Sequence Tagged Microsatellite Site Markers. Crop Sci. 51(6): 2412-2422.
  3. Erskine W. (1997) Lessons for breeders from land races of lentil. Euphytica. 93:107–112.
  4. Erskine W., Chandra S., Chaudhry M., Malik I. A., Sarker A., Sharma B., Tufail M., Tyagi M. C. (1998). A bottleneck in lentil: widening its genetic base in South Asia. Euphytica. 101(2):207-211.
  5. Ferguson M. E., Robertson L. D., Ford-Lloyd B. V., Newbury H. J., Maxted N. (1998). Contrasting genetic variation amongst lentil landraces from different geographical origins. Euphytica. 102:265–273.
  6. Gupta P. K., Balyan H. S., Varshney R. K., Gill K. S. (2013). Development and use of molecular markers for crop improvement. Plant Breed. 132:431–432.
  7. Gupta M., Verma B., Kumar N., Chahota R. K., Rathour R., Sharma S. K., Bhatia S. , Sharma T. R. (2012). Construction of inter subspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers. J Genet. 91:279–287.
  8. Hamwieh, A., Udupa, S. M., Sarker, A., Jung, C. and Baum, M. (2009). Development of new microsatellite markers and their application in the analysis of genetic diversity in lentils. Breeding Sci. 59(1):77-86.
  9. Kumar J., Pratap A., Solanki R. K., Gupta D. S., Goyal A., Chaturvedi S. K. (2011). Advances in genomics resources for improving food legume crops. J. Agril. Sci. 150 289–318. 
  10. Kumar, S., Barpetel, S., Kumar, J., Gupta, P. and Sarker, A. (2013). Global Lentil Production:Constraints and Strategies. SATSA MUKHOPATRA- Annual Technical Issue, 17:1-13.
  11. Kumar S., Hamweih A., Manickavelu A., Kumar J., Sharma T.R., Baum M. (2014). “Advances in lentil genomics,” In Legumes in Omics Era [eds Gupta S., Nadarajan N., Gupta D. S., editors]. Springer Science+Business Media), New York: 111–130.
  12. Ladizinski D., Braun D., Muehlbauer F. J. (1984). The biological species of the genus Lens. Bot Gaz.145:235–261.
  13. Rahman M. M., Sarker A., Kumar S., Ali A., Yadav N. K., Rahman L. (2009). Breeding for short season environments. In: Erskine W, Muehlbauer F, Sarker A, Sharma B, editors. The Lentil: Botany, Production and Uses. Wallingford: CAB International; pp. 121–136.
  14. Reddy M. R. K., Rathour R., Kumar N., Kathoch P., Sharma T. R. (2009). Cross-genera legume SSR markers for analysis of genetic diversity in Lens species. Plant Breed. 129:514–518.
  15. Udupa S. M., Robertson L. D., Weigand F., Baum M., Kahl G. (1999) Allelic variation at (TAA)n microsatellite loci in a world collection of chickpea (Cicer arietinum L.) germplasm. Mol Gen Genet. 261:354–363
  16. Varshney R. K., Close T. J., Singh N. K., Hoisington D. A., Cook D. R. (2009) Orphan legume crops enter the genomics era! Curr Opin Plant Biol. 11:1–9.
  17. Verma P., Sharma T.R., Srivastava P.S., Abdin M.Z., Bhatia S. (2014). Exploring genetic variability within lentil (Lens culinaris Medik.) and across related legumes using a newly developed set of microsatellite markers. Mol. Biol. Rep. 41:5607–5625 
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    Research Article

    volume 43 issue 5 (october 2020) : 617-622,   Doi: 10.18805/LR-4029

    Diversity analysis by SSR markers and morphological markers among Stemphylium blight tolerance genotypes with high yielding cultivars of Lentil (Lens Culinaris Medik)

    D
    Debarati Mandal
    P
    Prabir Kumar Bhattacharyya
    R
    Raju Das
    S
    Somnath Bhattacharyya
    1Department of Genetics and Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur-741252, Nadia, West Bangal.

    • Submitted18-04-2018|

    • Accepted05-06-2018|

    • First Online 20-08-2018|

    • doi 10.18805/LR-4029

    Cite article:- Mandal Debarati, Bhattacharyya Kumar Prabir, Das Raju, Bhattacharyya Somnath (2018). Diversity analysis by SSR markers and morphological markers among Stemphylium blight tolerance genotypes with high yielding cultivars of Lentil (Lens Culinaris Medik). Legume Research. 43(5): 617-622. doi: 10.18805/LR-4029.

    ABSTRACT

    Twelve Simple Sequence Repeat (SSR) markers along with twelve morphological markers were used to assess the diversity among thirteen screened genotypes both moderate resistant and susceptible against stemphylium blight of indigenous microsperma germplasm (i.e. pilosae) type of Lentil (Lens culinaris Medik.) at BCKV, West Bengal. Polymorphic Information Content (PIC) of SSR primers ranged from 0.77 (SSR 317-2) to 0.15 (PBA LC 0233). The maximum Jaccard’s similarity was recorded between L-4769 and Ranjan (approximately 100%) whereas minimum was between K-75 and VL 126 (0.11). Genotypes K-75 also showed less similarity with DL 14-2 and VL 151 (0.17). Another popular variety WBL 77 showed minimum similarity with moderately resistant genotypes VL 151 and DL 14-2 (0.17). While using morphological characters as the basis of diversity analysis, Euclidean coefficient revealed that high yielding cultivar Subrata was maximum diverse with genotype VL 151(7.12).  Asha also showed diversity with VL 151(6.31) whereas VL 151 had dissimilarity coefficient of 6.21 with K-75. Precoz and VL 126 has dissimilarity coefficient of 6.19. On the basis of above findings it could be said that diverse parental pairs VL 151 & K 75 and VL 151 and Subrata / Asha could be used in hybridization programme or biotic stress breeding programme for developing Stemphylium resistant lentil cultivars in future.

    REFERENCES

    1. Abe, J., Xu D. H., Suzuki Y., Kanazawa A. and Shimanmoto Y. (2003). Soybean germplasm pools in Asia revealed by nuclear SSRs. Theor Appl Genet. 106:445–453.
    2. Datta, S., Tiwari S., Kaashyap M., Gupta P. P., Choudhury P. R., Kumari J., Kumar S. (2011). Genetic Similarity Analysis in Lentil Using Cross-Genera Legume Sequence Tagged Microsatellite Site Markers. Crop Sci. 51(6): 2412-2422.
    3. Erskine W. (1997) Lessons for breeders from land races of lentil. Euphytica. 93:107–112.
    4. Erskine W., Chandra S., Chaudhry M., Malik I. A., Sarker A., Sharma B., Tufail M., Tyagi M. C. (1998). A bottleneck in lentil: widening its genetic base in South Asia. Euphytica. 101(2):207-211.
    5. Ferguson M. E., Robertson L. D., Ford-Lloyd B. V., Newbury H. J., Maxted N. (1998). Contrasting genetic variation amongst lentil landraces from different geographical origins. Euphytica. 102:265–273.
    6. Gupta P. K., Balyan H. S., Varshney R. K., Gill K. S. (2013). Development and use of molecular markers for crop improvement. Plant Breed. 132:431–432.
    7. Gupta M., Verma B., Kumar N., Chahota R. K., Rathour R., Sharma S. K., Bhatia S. , Sharma T. R. (2012). Construction of inter subspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers. J Genet. 91:279–287.
    8. Hamwieh, A., Udupa, S. M., Sarker, A., Jung, C. and Baum, M. (2009). Development of new microsatellite markers and their application in the analysis of genetic diversity in lentils. Breeding Sci. 59(1):77-86.
    9. Kumar J., Pratap A., Solanki R. K., Gupta D. S., Goyal A., Chaturvedi S. K. (2011). Advances in genomics resources for improving food legume crops. J. Agril. Sci. 150 289–318. 
    10. Kumar, S., Barpetel, S., Kumar, J., Gupta, P. and Sarker, A. (2013). Global Lentil Production:Constraints and Strategies. SATSA MUKHOPATRA- Annual Technical Issue, 17:1-13.
    11. Kumar S., Hamweih A., Manickavelu A., Kumar J., Sharma T.R., Baum M. (2014). “Advances in lentil genomics,” In Legumes in Omics Era [eds Gupta S., Nadarajan N., Gupta D. S., editors]. Springer Science+Business Media), New York: 111–130.
    12. Ladizinski D., Braun D., Muehlbauer F. J. (1984). The biological species of the genus Lens. Bot Gaz.145:235–261.
    13. Rahman M. M., Sarker A., Kumar S., Ali A., Yadav N. K., Rahman L. (2009). Breeding for short season environments. In: Erskine W, Muehlbauer F, Sarker A, Sharma B, editors. The Lentil: Botany, Production and Uses. Wallingford: CAB International; pp. 121–136.
    14. Reddy M. R. K., Rathour R., Kumar N., Kathoch P., Sharma T. R. (2009). Cross-genera legume SSR markers for analysis of genetic diversity in Lens species. Plant Breed. 129:514–518.
    15. Udupa S. M., Robertson L. D., Weigand F., Baum M., Kahl G. (1999) Allelic variation at (TAA)n microsatellite loci in a world collection of chickpea (Cicer arietinum L.) germplasm. Mol Gen Genet. 261:354–363
    16. Varshney R. K., Close T. J., Singh N. K., Hoisington D. A., Cook D. R. (2009) Orphan legume crops enter the genomics era! Curr Opin Plant Biol. 11:1–9.
    17. Verma P., Sharma T.R., Srivastava P.S., Abdin M.Z., Bhatia S. (2014). Exploring genetic variability within lentil (Lens culinaris Medik.) and across related legumes using a newly developed set of microsatellite markers. Mol. Biol. Rep. 41:5607–5625 
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