Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Legume Research, volume 43 issue 5 (october 2020) : 627-633

A comparative biochemical evaluation of in vivo and in vitro propagated Alhagi maurorum: an important medicinal plant

Ashish Malik, Krishan Sehrawat Anil Ahlawat, Anita R. Sehrawat
1Department of Botany, Maharshi Dayanand University, Rohtak-124 001, Haryana, India.
  • Submitted08-03-2018|

  • Accepted03-04-2018|

  • First Online 16-07-2018|

  • doi 10.18805/LR-4016

Cite article:- Malik Ashish, Ahlawat Anil Sehrawat Krishan, Sehrawat R. Anita (2018). A comparative biochemical evaluation of in vivo and in vitro propagated Alhagi maurorum: an important medicinal plant. Legume Research. 43(5): 627-633. doi: 10.18805/LR-4016.
Medicinal plants are of great interest in the field of biotechnology as most of the drug industries depend in part on plants for production of pharmaceutical compounds. Biological activities of the phyoconstituents in plants could be enhanced by manipulation of the culture conditions. In the present investigation leaves and nodal segments of Alhagi maurorum were cultured on MS medium with BAP, Kinetin, NAA and other adjuvants. MS medium with BAP (1.0 and 2.0 mg/l) alone and in combination with NAA (0.1 mg/l was good for initiation of calli and bud formation. BAP (2.0 and 5.0mg/l) with NAA(0.1mg/l) plus kinetin 0.25mg/l with ascorbic acid 50mg/l and adenine sulphate, citric acid and arginine 25mg/l  each was found to be the best for bud proliferation and shoot multiplication. Best rooting was found on MS medium supplemented with IAA and IBA (0.5mg/ leach).  Cultured materials at different growth phase were evaluated for their biochemical estimation of primary metabolites quantitatively. Maximum content of carbohydrates, total proteins and amino acids were noticed in leaves of field grown plants and shoots of regenerated plantlets. It is further concluded that Alhagi maurorum serve as a rich source of primary metabolites which can be used as raw materials in industry. 
  1. Agarwal, T., Gupta, A.K., Patel, A.K., Shekhawat, N.S. (2015). Micropropagation and validation of genetic homogeneity of Alhagi maurorum using SCoT, ISSR and RAPD markers Plant Cell Tiss Organ Cult., 120:313–323
  2. Ahmad, M., Khan, M.A., Marwat, S.K. (2009). Useful medicinal flora enlisted in Holy Quran and Ahdith. Am Eurasian J Agric Environ Sci., 5(1): 126-140.
  3. Ana, M.F., and Maria, S.P. (2000). Organogenesis from internode derived nodules of Humulus lupulus Var. Nugget (Cannabinaceae): Histological studies and changes in the sxzstarch content. American Journal of Botany, 87: 971-979.
  4. Barik, D.P., Naik, S.K., Mohapatra, U., Chand, P.K. (2004). High frequency plant regeneration by in vitro shoot proliferation in cotyledonary node explants of grasspea (Lathyrus sativus L.). In Vitro Cell. Dev. Biol. Plant., 40: 467–470.
  5. Babeet, S. T., Choudhary, R. and Vergia, R.V. (2010). In vitro and In vivo comparative study of Primary Metabolites and Antioxidant Activity in Spilanthes acmella Merr. International Journal of Biotechnoligy and Biochemistry, 6:
  6. Dubois, M.K., Gilles, A., Hanito, J.K., Rebers, P.A., and Smith, F. (1956) Calorimetric Methods for determination of sugars and related Substances. Analytical Chem., 28: 350-356.
  7. Engelmann, N.J., Campbell, J.K., Rogers, R.B., Rupassara, S.I., Garlick, P.J., Lila, M.A., Erdman, J.W. (2010). Screening and selection of high carotenoid producing in vitro tomato cell culture lines for [13C]-Carotenoid Production J Agric Food Chem., 58(18): 9979–9987. 
  8. Entwistle, G., Bachlor S., Booth F., Walker K. (1998) Economics of starch production in UK. Industrial Crop Prod., 7(2-3): 175-186.
  9. Hansen, J., Moller, I. (1975). Percolation of starch and soluble carbohydrates from plant tissue for quantitative determination with anthrone. Anal Biochem.68(1): 87-94.
  10. Hassanein, A.M. (2004). Hormonal Requirements Induced Different Regeneration Pathways in Alhagi graecorum. Jrnl of Plant Biotech. 6(3): 171-179.
  11. Hassanein, A.M., and Mazen, A.M.A. (2001). Adventitious bud formation in Alhagi graecorum Plant Cell, Tissue and Organ Culture., 65: 31–35.
  12. Kerr, H.D., Robacker, W.C., and Muzik, T.J. (1965). Characteristics and control of camelthorn Weeds, 13:156-163.
  13. Khalafalla, M.M., Hattori, K. (1999). A combination of thidiazuron and benzyladenine promotes multiple shoot production from cotyledonary node explants of faba bean (Vicia faba L.). Plant Growth Regul., 27: 145–148.
  14. Khan, S., Ahmad, F., Ali, F., Khan, H., Khan, A., Swati, Z.A. (2011). Callus induction via different growth regulators from cotyledon explants of indigenous chick pea (Cicer arietinum L.) cultivars KK-1 and Hassan-2K. Af J of Biotech., 10(40): 7825-7830.
  15. Lichtenthaler, H.K., Wellburn, A.R., (1985) Determination of total carotenoids and chlorophylls A and B of leaf in different solvents. Biol. Soc. Trans., 11: 591-592.
  16. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with the folin phenol reagent. J Biol Chem., 193: 265–75.
  17. Mahendran, D., Srinivasan, S., Thiyagarajan, M., and Venkatachalam, P. (2012). Biochemical analysis between in vivo and in vitro regenerated plant leaves of Solanum nigrum L. Plant Cell Biotechnology and Molecular Bio., 13 (3-4):147-152.
  18. Malabadi, R.B., Nataraja, K. (2001). Shoot regeneration in leaf explants of Clitoria ternatea L. cultured in vitro. Phytomorphology., 51: 169–171.
  19. Manohari, S.D., Indra. J.S.M., and Muthuchelian, K. (2011). Biochemical characterization of mother plant and tissue cultured plants of Adenanthera pavonina (Fabaceae). J.Biosci. Res., 2(4): 225-231.
  20. Miller, G.L. (1959) Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal. Chem., 31 (3): 426–428
  21. Murashige, T., Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum., 15: 473–497.
  22. Rafi, S., Kamili, A.N., Ganai, B.A., Mir, M.Y., Parray, J.A. (2012). In vitro Culture and Biochemical Attributes of Bergenia ciliate (Haw.) Sternb. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci., DOI 10.1007/s40011-016-0797-9. 
  23. Sadasivam, S., Manickam, A. (1992). Biochemical methods for agricultural sciences. 12-13.
  24. Singh, R., Singal, H.R., Goyal, S.C. (2006). Biochemical changes during shoot differentiation in callus cultures of Chlorophytum borivilianum Sant. Et Fernand. Indian J Plant Physio. 11: 130-135.
  25. Singh, S., Tanwer, B.S., Khan, M. (2011). Callus induction and in vivo and in vitro comparative study of primary metabolites of Withania somnifera. Adv. Appl. Sci. Res., 2(3): 47-52.
  26. Sivananthan, M., and Elamaran, M. (2013). In vitro evaluation of antibacterial activity of chloroform extract Andrographis paniculata leaves and roots, Durio zibethinus wood bark and Psidium guajava leaves against selected bacterial strains. Int J Biomol Biomed., 3: 12-19.
  27. Srivastava, J., Lambert, J., and Vietmeyer, N. (1995). Medicinal plants: an expanding role in development, World Bank technical paper no. 320, Washington, DC: World Bank Agriculture and Forestry Systems.
  28. Talreja, T. (2011). Biochemical Estimation of Three Primary Metabolites from Medicinally Important Plant Moringa oleifera. Inter J of Pharml Sci Rev and Res., 7(2): 186-188.
  29. Wang, Y.M., Wang, B.J., Luo, D., Jia, F.J. (2001). Regeneration of plants from callus tissue of hairy roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi. Cell research, 11(4): 279-284. 

Editorial Board

View all (0)