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Research Article

volume 43 issue 4 (august 2020) : 586-591,   Doi: 10.18805/LR-4001

Evaluation of native Rhizobium leguminosarum bv. viceae of pea plants (Pisum sativum L.) for nodulation efficiency and yield promotion in lentil crop (Lens culnaris Medik.) under acid soil

C
Christy B.K. Sangma
D
Dwipendra Thakuria
1SNRM, College of Post Graduate Studies, Central Agricultural University-Imphal, Umiam-793 103, Meghalaya, India

  • Submitted07-02-2018|

  • Accepted09-04-2018|

  • First Online 16-07-2018|

  • doi 10.18805/LR-4001

Cite article:- Sangma B.K. Christy, Thakuria Dwipendra (2018). Evaluation of native Rhizobium leguminosarum bv. viceae of pea plants (Pisum sativum L.) for nodulation efficiency and yield promotion in lentil crop (Lens culnaris Medik.) under acid soil. Legume Research. 43(4): 586-591. doi: 10.18805/LR-4001.

ABSTRACT

Most leguminous crops including lentil require neutral to slightly acidic range of soil pH for favourable nodulation and nitrogen fixation. The quest for acid soil compatible Rhizobium leguminosarum bv. viceae strain is important for promotion of lentil crop under rice-lentil rotation in acid soil regions. In this investigation, native R. leguminosarum strains were isolated from the nodules of pea plants grown in pots using acid soil and isolated native Rhizobium strains were confirmed on CRYEMA (congo red Yeast Extract Mannitol Agar) media. Four native R. leguminosarum strains (NR1, NR2, NR3 and NR4) obtained from four acid soil types were further screened for nodulation efficiency and yield attributes of lentil crop against a reference exotic strain R. leguminosarum CK1 (ER). The results indicated that the performance of native Rhizobium strain (NR2) was the best among native isolates and performed on par with the reference strain (CK1) in acid soils of Meghalaya.

REFERENCES

  1. Baraibar, A., Frioni, L., Guedes, M.E. and Ljunggren, H. (1999). Symbiotic effectiveness and ecological characterization of indigenous Rhizobium loti populations in Uruguay. Pesquisa Agropeccuaria Brasileira, 34(6): 1011-1017. 
  2. Bray, R.H. and Kurtz, L.T. (1945). Determination of total, organic and available form of phosphorus in soil. Soil Science, 59: 39-45.
  3. Casida, L.E.Jr., Klein D.A., and Sautoro, T. (1964). Soil dehydrogenase activity. Soil Science, 98: 371-376.
  4. FAO (2012). FAOSTAT http://faostat.fao.org/, FAO, Rome, Italy. 
  5. Hanlan, T.G., Ball, R.A. and Vandenberg, A. (2006). Canopy growth and biomass partitioning to yield in short-season lentil. Canadian Journal of Plant Science, 86: 109-119.
  6. Iqbal, M.M., Mohammad, W., Shah, M.S., Nawaz, H. and Idris, M. (2002). Nitrogen fixation by lentil in farmers’ field in rainfed dry conditions. 17th WCSS, Thailand.
  7. Jida, M. and Assefa, F. (2014). Effects of acidity on growth and symbiotic performance of Rhizobium leguminosarum bv. viciae strains isolated from Faba bean producing areas of Ethiopia. Science, Technology and Arts Research Journal, 3(2): 26-33.
  8. Khanna, V. and Sharma, P. (2011). Potential for enhancing lentil (Lens culinaris) productivity by co-inoculation with PSB, plant growth-promoting rhizobacteria and Rhizobium. Indian Journal of Agricultural Science, 81(10): 932–4.
  9. Muehlbauer, F.J., Cubero, J.J., and Summerfield, R.J. (1985). Lentil (Lens culinaris Medk.). In: Grain legume crops. Summerfield, R.J. and Roberts, E.H. (eds.) Collins, London, UK. Pp. 266-311.
  10. Niste, M., Vidican, R., Puia, C., Rotar, I. and Rodica, P.O.P. (2015). Isolation and Biochemical Characterization of Rhizobium Leguminosarum bv. trifolii and Sinorhizobium Meliloti using API 20NE and API 20E. Bulletin UASVM Agriculture, 72 (1).
  11. Singh, A.K., Singh, S.S., Prakash, V., Kumar, S. and Dwivedi, S.K. (2015). Pulses Production in India: Present Status, Bottleneck and Way Forward. Journal of Agrisearch, 2(2): 75-83.
  12. Subbiah, B.V. and Asija, C.L. (1956). A rapid procedure for estimation of available nitrogen in soils. Current Science, 25: 259-260.
  13. Tabatabai, M.A. and Bremner, J.M. (1969). Use of nitrophenylophosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, 1: 301-307. 
  14. Upadhayay, S.P., Pareek, N. and Mishra, G. (2015). Isolation and biochemical characterization of Rhizobium strains from nodules of lentil and pea in Tarai agro-ecosystem, Pantnagar, India. Nusantara Bioscience, 7(2): 73-76. 
  15. Vincent, J.M. (1970). A manual of practical study of Root nodule Bacteria. Blackwell Scientific publication, Oxford, London.
  16. Waheed, A., Afzal A., Sultan, T., Rahman, I.U., Sohail, Ijaz, F., Manan, S., Asma, Zia, M.A., Khan, K. and Shah, A. (2014). Isolation and biochemical characterization of Rhizobium from pea crop at Swabi. International Journal of Biosciences, 4(8): 231-240. 
  17. Walkley, A. and Black, I.A. (1947). An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science, 37: 29-38.
  18. Yahyaabadi, M. (2008). Evaluation of nitrogen fixation potential and nutrients uptaking in some common bean symbiosis bacteria. Proceeding of 10th Iranian Congress of Crop Production and Plant Breeding, Karaj, Iran, pp. 75. 
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    Research Article

    volume 43 issue 4 (august 2020) : 586-591,   Doi: 10.18805/LR-4001

    Evaluation of native Rhizobium leguminosarum bv. viceae of pea plants (Pisum sativum L.) for nodulation efficiency and yield promotion in lentil crop (Lens culnaris Medik.) under acid soil

    C
    Christy B.K. Sangma
    D
    Dwipendra Thakuria
    1SNRM, College of Post Graduate Studies, Central Agricultural University-Imphal, Umiam-793 103, Meghalaya, India

    • Submitted07-02-2018|

    • Accepted09-04-2018|

    • First Online 16-07-2018|

    • doi 10.18805/LR-4001

    Cite article:- Sangma B.K. Christy, Thakuria Dwipendra (2018). Evaluation of native Rhizobium leguminosarum bv. viceae of pea plants (Pisum sativum L.) for nodulation efficiency and yield promotion in lentil crop (Lens culnaris Medik.) under acid soil. Legume Research. 43(4): 586-591. doi: 10.18805/LR-4001.

    ABSTRACT

    Most leguminous crops including lentil require neutral to slightly acidic range of soil pH for favourable nodulation and nitrogen fixation. The quest for acid soil compatible Rhizobium leguminosarum bv. viceae strain is important for promotion of lentil crop under rice-lentil rotation in acid soil regions. In this investigation, native R. leguminosarum strains were isolated from the nodules of pea plants grown in pots using acid soil and isolated native Rhizobium strains were confirmed on CRYEMA (congo red Yeast Extract Mannitol Agar) media. Four native R. leguminosarum strains (NR1, NR2, NR3 and NR4) obtained from four acid soil types were further screened for nodulation efficiency and yield attributes of lentil crop against a reference exotic strain R. leguminosarum CK1 (ER). The results indicated that the performance of native Rhizobium strain (NR2) was the best among native isolates and performed on par with the reference strain (CK1) in acid soils of Meghalaya.

    REFERENCES

    1. Baraibar, A., Frioni, L., Guedes, M.E. and Ljunggren, H. (1999). Symbiotic effectiveness and ecological characterization of indigenous Rhizobium loti populations in Uruguay. Pesquisa Agropeccuaria Brasileira, 34(6): 1011-1017. 
    2. Bray, R.H. and Kurtz, L.T. (1945). Determination of total, organic and available form of phosphorus in soil. Soil Science, 59: 39-45.
    3. Casida, L.E.Jr., Klein D.A., and Sautoro, T. (1964). Soil dehydrogenase activity. Soil Science, 98: 371-376.
    4. FAO (2012). FAOSTAT http://faostat.fao.org/, FAO, Rome, Italy. 
    5. Hanlan, T.G., Ball, R.A. and Vandenberg, A. (2006). Canopy growth and biomass partitioning to yield in short-season lentil. Canadian Journal of Plant Science, 86: 109-119.
    6. Iqbal, M.M., Mohammad, W., Shah, M.S., Nawaz, H. and Idris, M. (2002). Nitrogen fixation by lentil in farmers’ field in rainfed dry conditions. 17th WCSS, Thailand.
    7. Jida, M. and Assefa, F. (2014). Effects of acidity on growth and symbiotic performance of Rhizobium leguminosarum bv. viciae strains isolated from Faba bean producing areas of Ethiopia. Science, Technology and Arts Research Journal, 3(2): 26-33.
    8. Khanna, V. and Sharma, P. (2011). Potential for enhancing lentil (Lens culinaris) productivity by co-inoculation with PSB, plant growth-promoting rhizobacteria and Rhizobium. Indian Journal of Agricultural Science, 81(10): 932–4.
    9. Muehlbauer, F.J., Cubero, J.J., and Summerfield, R.J. (1985). Lentil (Lens culinaris Medk.). In: Grain legume crops. Summerfield, R.J. and Roberts, E.H. (eds.) Collins, London, UK. Pp. 266-311.
    10. Niste, M., Vidican, R., Puia, C., Rotar, I. and Rodica, P.O.P. (2015). Isolation and Biochemical Characterization of Rhizobium Leguminosarum bv. trifolii and Sinorhizobium Meliloti using API 20NE and API 20E. Bulletin UASVM Agriculture, 72 (1).
    11. Singh, A.K., Singh, S.S., Prakash, V., Kumar, S. and Dwivedi, S.K. (2015). Pulses Production in India: Present Status, Bottleneck and Way Forward. Journal of Agrisearch, 2(2): 75-83.
    12. Subbiah, B.V. and Asija, C.L. (1956). A rapid procedure for estimation of available nitrogen in soils. Current Science, 25: 259-260.
    13. Tabatabai, M.A. and Bremner, J.M. (1969). Use of nitrophenylophosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, 1: 301-307. 
    14. Upadhayay, S.P., Pareek, N. and Mishra, G. (2015). Isolation and biochemical characterization of Rhizobium strains from nodules of lentil and pea in Tarai agro-ecosystem, Pantnagar, India. Nusantara Bioscience, 7(2): 73-76. 
    15. Vincent, J.M. (1970). A manual of practical study of Root nodule Bacteria. Blackwell Scientific publication, Oxford, London.
    16. Waheed, A., Afzal A., Sultan, T., Rahman, I.U., Sohail, Ijaz, F., Manan, S., Asma, Zia, M.A., Khan, K. and Shah, A. (2014). Isolation and biochemical characterization of Rhizobium from pea crop at Swabi. International Journal of Biosciences, 4(8): 231-240. 
    17. Walkley, A. and Black, I.A. (1947). An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science, 37: 29-38.
    18. Yahyaabadi, M. (2008). Evaluation of nitrogen fixation potential and nutrients uptaking in some common bean symbiosis bacteria. Proceeding of 10th Iranian Congress of Crop Production and Plant Breeding, Karaj, Iran, pp. 75. 
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