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Research Article

volume 41 issue 4 (august 2018) : 624-628,   Doi: 10.18805/LR-399

Characterization of rhizobia from root nodule and rhizosphere of Vicia faba in Algeria

B
Benselama Amel
T
Tellah Sihem
O
Ourem Faiza
O
Ounane Sidi Mohamed
1Laboratory of Integrative Improvement of Plant Production, School National Superior of Agronomy AIPV (code C2711100). El-Harrach, Algeria.

  • Submitted15-12-2017|

  • Accepted18-03-2018|

  • First Online 22-08-2018|

  • doi 10.18805/LR-399

Cite article:- Amel Benselama, Sihem Tellah, Faiza Ourem, Mohamed Sidi Ounane (2018). Characterization of rhizobia from root nodule and rhizosphere of Vicia faba in Algeria. Legume Research. 41(4): 624-628. doi: 10.18805/LR-399.

ABSTRACT

Nitrogen fixation resulting from mutual symbiosis of rhizobia and cultivated legume plants is therefore critical to food security as it directly affects agricultural production. Biological Nitrogen Fixation (BNF) can be an important factor in sustainable agriculture. A collection of 20 isolates from fresh Nodules of the legume plant Vicia faba was isolated from five soil of Algeria. The soil from each region has undergone physical-chemical analysis : (granulometry, total carbon, organic matter, pH, and electrical conductivity, determination of available phosphorus and determination of total nitrogen). A media viz., Glucose-Peptone Agar (GPA), Congo red, Yeast Mannitol Agar (YMA) containing 2% NaCl were employed to make presumptive decisions on the recognition and classification of the isolated bacterial strains. All the isolates were found with poor absorption of dye Congo red and little or no growth on the media of GPA and without altering the pH. Almost all of the isolates exhibit good growth on 2% NaCl, poor growth on GPA, thus confirming the rhizobia. After biochemical tests like catalase test and citrate utilization test isolates were confirmed. The presence of rhizobia on root nodules of leguminous plant. 

REFERENCES

  1. Abdel,L. (2008). Effect of seed size and plant spacing on yield and yield components of Faba bean (Vicia fava L.). Res. J Agric. Biolog. Sci.,4 :146-148.
  2. Arora, D. (2003). The Text Book of Microbiology. New Delhi, P 41-48. 
  3. Brar, S., Paramjit, S. (2017). Response of cluster bean (Cyamopsis tetragonoloba L. Taub.) cultivars to dual inoculation with fixing and phosphorous solubizing bacteria. Legume Research., 40: 100-104
  4. Bra,y, R., Kurtz, L. (1945). Determination of total organic and available forms of phosphorus in soils. Soil Sci., 59 :39–45. 
  5. Bremner, J. (1965). Total nitrogen. In: Methods of Soil Analysis Black CA, editor., P 1149–1178.
  6. Bond, D. (1976). Field bean, Vicia faba. In : Evolution of Crop Plants. Longman, London, UK, P 179-182.
  7. Castro, I., Ferreira, E., McGrat, S. (2003). Survival and plasmid stability of rhizobia introduced into a contaminated soil. Soil Biol., 35 : 49-54. 
  8. Cappuccino, J., Sherman, N. (1992). Microbiology: A Laboratory Manual, New York, P 125-179. 
  9. Day, P. (1965). Methods of Soil Analysis. Agronomy., 9:545–567.
  10. Dubey, R., Maheshwari, K., Kumar, H., Choure, K. (2010). Assessment of diversity and plant growth promoting attributes of rhizobia isolated from Cajanus cajan L. African J. Biotechnology., 9: 8619-8629. 
  11. Gadrat, J. (1997). Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria. Plant Soil., 8: 15-21.
  12. Geniaux, E., Laguerre, G., Amarge, N. (1993). Comparison of geographically distant populations of Rhizobium isolated from root nodules of Phaseolus vulgaris. Mol. Ecol., 2: 295–302. 
  13. Hanelt, P., Mettin, D. (1989). Biosystematics of the genus Vicia L. (Leguminosae). Annu. Rev. Ecol. Syst, 20 :199–223.
  14. Handley, B., Hedges, A., Beringer, J. (1998). Importance of host plants for detecting the population diversity of Rhizobium leguminosarum biovar viciae in soil. Soil Biology., 3 : 241-249. 
  15. Harlan, J. (1969). Ethiopia: A centre of diversity. Econ. Bot., 23 :309-14.
  16. Hawtin, G., Hebblethpiait, P. (1983). Background and history of faba bean production, P 3-22.
  17. Hoffmann, D., Jiang, Q., Men, A., Kinkema, M., Gresshoff, P. (2007). Nodulation deficiency caused by fast neutron mutagenesis of the model legume Lotus japonicus. J. Plant Physiol., 164 :460–469. 
  18. Horst, I., Welham, T., Kelly, S., Kaneko, T., Sato, S., Tabata, S., Parniske, M., Wang, T. (2007). Tilling mutants of Lotus japonicus reveal that nitrogen assimilation and fixation can occur in the absence of noduleenhanced sucrose synthase. Plant Physiol., 144 :806–820.
  19. Jing, L., Yu-Wei, L. (2016). Soluble sugars and myo-inositol phosphates during germination and seedling growth of green and white faba bean (Vicia faba L.). Legume Research., 40 : 47-54.
  20. Jordan, D. (1982). Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria frorn legurninous plants. Int. J. Syst. Bacteriol., 32 : 136-13
  21. Kanimozhi, K., Panneerselvam, A. (2011). Growth and fertility of five stocks of field beans grown under three temperatures regimes and the effect of natural water stress on seed index of a collection of Vicia faba L. Agronomy and Crop Science., 2 :525-536.
  22. Kucuk, C., Kivanç, M., Kinaci, E. (2006). Characterization of Rhizobium Sp. isolated from Bean. Turk J Biol., 30 : 127-132. 
  23. Mahana, E. (2000). Diversity of Rhizobium-Phaseolus vulgaris symbiosis: overview and perspectives. Plant Soil., 252:11-23. 
  24. McVicar, R., Panchuk, K., Brenzil, C., Hartley, S., Pearse, P. (2005). Faba Bean in Sasktchewan. Saskatchewan Agriculture, Food and Rural Revitalization. University of Saskatchewan, Vandenberg, P11.
  25. Mihailovic, V., Mikic, A., Cupina, B., Eric, P. (2005). Field pea and vetches in serbia and Montenegro. Grain Legumes., 44 :25-26.
  26. Naqvi, H. (1984). Cultivation under the Sultans of Delhi. Indian J. History. Sci., 19 :329–340.
  27. Ngwenya, N., Zharare1, A., Basson, M., Mthembu, S. (2016). Phenotypic characterisation of rhizobia nodulating different morphotypes of Vachellia karroo. Legume Research An International Journal., 39 : 722-728.
  28. Oplinger, E. (1982). Fababeans Field Crops. Madison, P53-70.
  29. Peech, M. (1965). Methods of soil analysis Chemical and microbial properties. Madison, P 914–926.
  30. Perret, X., Staehelin, C., Broughton, W. (2000). Molecular basis of symbiotic promiscuity. Mol. Biol., 64: 180-201.
  31. Singh, A., Bhatt, B. (2012). Faba bean: unique germplasm explored and identified. Hort. Flora Res. Spectrum. Plant Soil., 1 :267-269
  32. Somasegaran, P. and Hoben, H. J. (1994). Collecting nodules and isolating rhizobia in : Handbook for Rhizobia, Methods in Legume-    Rhizobium Technology, Springer. P 7-23.
  33. Vered, Y., Grosskopf, I., Palevitch, D., Harsat, A., Charach, G., Weintraub, M., Graff, E. (1997). The influence of Vicia faba (broad bean) seedlings on urinary sodium excretion. Planta Med., 63 :237-40.
  34. Vincent, J. (1970). A manual for the practical study of root-nodule bacterial. IPB handbook, Oxford: P 64.
  35. Walkley, A., Black, (1934). A. An examination of the Degtjareff methods for determining soil organic matter and a proposed modification of the chromic and titration method. Soil Sci., 37:29–38. 
  36. Zohary, D., Hopf, M. (1973). Domestication of pulses in the old world. Science., 182 :887-894 
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    Research Article

    volume 41 issue 4 (august 2018) : 624-628,   Doi: 10.18805/LR-399

    Characterization of rhizobia from root nodule and rhizosphere of Vicia faba in Algeria

    B
    Benselama Amel
    T
    Tellah Sihem
    O
    Ourem Faiza
    O
    Ounane Sidi Mohamed
    1Laboratory of Integrative Improvement of Plant Production, School National Superior of Agronomy AIPV (code C2711100). El-Harrach, Algeria.

    • Submitted15-12-2017|

    • Accepted18-03-2018|

    • First Online 22-08-2018|

    • doi 10.18805/LR-399

    Cite article:- Amel Benselama, Sihem Tellah, Faiza Ourem, Mohamed Sidi Ounane (2018). Characterization of rhizobia from root nodule and rhizosphere of Vicia faba in Algeria. Legume Research. 41(4): 624-628. doi: 10.18805/LR-399.

    ABSTRACT

    Nitrogen fixation resulting from mutual symbiosis of rhizobia and cultivated legume plants is therefore critical to food security as it directly affects agricultural production. Biological Nitrogen Fixation (BNF) can be an important factor in sustainable agriculture. A collection of 20 isolates from fresh Nodules of the legume plant Vicia faba was isolated from five soil of Algeria. The soil from each region has undergone physical-chemical analysis : (granulometry, total carbon, organic matter, pH, and electrical conductivity, determination of available phosphorus and determination of total nitrogen). A media viz., Glucose-Peptone Agar (GPA), Congo red, Yeast Mannitol Agar (YMA) containing 2% NaCl were employed to make presumptive decisions on the recognition and classification of the isolated bacterial strains. All the isolates were found with poor absorption of dye Congo red and little or no growth on the media of GPA and without altering the pH. Almost all of the isolates exhibit good growth on 2% NaCl, poor growth on GPA, thus confirming the rhizobia. After biochemical tests like catalase test and citrate utilization test isolates were confirmed. The presence of rhizobia on root nodules of leguminous plant. 

    REFERENCES

    1. Abdel,L. (2008). Effect of seed size and plant spacing on yield and yield components of Faba bean (Vicia fava L.). Res. J Agric. Biolog. Sci.,4 :146-148.
    2. Arora, D. (2003). The Text Book of Microbiology. New Delhi, P 41-48. 
    3. Brar, S., Paramjit, S. (2017). Response of cluster bean (Cyamopsis tetragonoloba L. Taub.) cultivars to dual inoculation with fixing and phosphorous solubizing bacteria. Legume Research., 40: 100-104
    4. Bra,y, R., Kurtz, L. (1945). Determination of total organic and available forms of phosphorus in soils. Soil Sci., 59 :39–45. 
    5. Bremner, J. (1965). Total nitrogen. In: Methods of Soil Analysis Black CA, editor., P 1149–1178.
    6. Bond, D. (1976). Field bean, Vicia faba. In : Evolution of Crop Plants. Longman, London, UK, P 179-182.
    7. Castro, I., Ferreira, E., McGrat, S. (2003). Survival and plasmid stability of rhizobia introduced into a contaminated soil. Soil Biol., 35 : 49-54. 
    8. Cappuccino, J., Sherman, N. (1992). Microbiology: A Laboratory Manual, New York, P 125-179. 
    9. Day, P. (1965). Methods of Soil Analysis. Agronomy., 9:545–567.
    10. Dubey, R., Maheshwari, K., Kumar, H., Choure, K. (2010). Assessment of diversity and plant growth promoting attributes of rhizobia isolated from Cajanus cajan L. African J. Biotechnology., 9: 8619-8629. 
    11. Gadrat, J. (1997). Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria. Plant Soil., 8: 15-21.
    12. Geniaux, E., Laguerre, G., Amarge, N. (1993). Comparison of geographically distant populations of Rhizobium isolated from root nodules of Phaseolus vulgaris. Mol. Ecol., 2: 295–302. 
    13. Hanelt, P., Mettin, D. (1989). Biosystematics of the genus Vicia L. (Leguminosae). Annu. Rev. Ecol. Syst, 20 :199–223.
    14. Handley, B., Hedges, A., Beringer, J. (1998). Importance of host plants for detecting the population diversity of Rhizobium leguminosarum biovar viciae in soil. Soil Biology., 3 : 241-249. 
    15. Harlan, J. (1969). Ethiopia: A centre of diversity. Econ. Bot., 23 :309-14.
    16. Hawtin, G., Hebblethpiait, P. (1983). Background and history of faba bean production, P 3-22.
    17. Hoffmann, D., Jiang, Q., Men, A., Kinkema, M., Gresshoff, P. (2007). Nodulation deficiency caused by fast neutron mutagenesis of the model legume Lotus japonicus. J. Plant Physiol., 164 :460–469. 
    18. Horst, I., Welham, T., Kelly, S., Kaneko, T., Sato, S., Tabata, S., Parniske, M., Wang, T. (2007). Tilling mutants of Lotus japonicus reveal that nitrogen assimilation and fixation can occur in the absence of noduleenhanced sucrose synthase. Plant Physiol., 144 :806–820.
    19. Jing, L., Yu-Wei, L. (2016). Soluble sugars and myo-inositol phosphates during germination and seedling growth of green and white faba bean (Vicia faba L.). Legume Research., 40 : 47-54.
    20. Jordan, D. (1982). Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria frorn legurninous plants. Int. J. Syst. Bacteriol., 32 : 136-13
    21. Kanimozhi, K., Panneerselvam, A. (2011). Growth and fertility of five stocks of field beans grown under three temperatures regimes and the effect of natural water stress on seed index of a collection of Vicia faba L. Agronomy and Crop Science., 2 :525-536.
    22. Kucuk, C., Kivanç, M., Kinaci, E. (2006). Characterization of Rhizobium Sp. isolated from Bean. Turk J Biol., 30 : 127-132. 
    23. Mahana, E. (2000). Diversity of Rhizobium-Phaseolus vulgaris symbiosis: overview and perspectives. Plant Soil., 252:11-23. 
    24. McVicar, R., Panchuk, K., Brenzil, C., Hartley, S., Pearse, P. (2005). Faba Bean in Sasktchewan. Saskatchewan Agriculture, Food and Rural Revitalization. University of Saskatchewan, Vandenberg, P11.
    25. Mihailovic, V., Mikic, A., Cupina, B., Eric, P. (2005). Field pea and vetches in serbia and Montenegro. Grain Legumes., 44 :25-26.
    26. Naqvi, H. (1984). Cultivation under the Sultans of Delhi. Indian J. History. Sci., 19 :329–340.
    27. Ngwenya, N., Zharare1, A., Basson, M., Mthembu, S. (2016). Phenotypic characterisation of rhizobia nodulating different morphotypes of Vachellia karroo. Legume Research An International Journal., 39 : 722-728.
    28. Oplinger, E. (1982). Fababeans Field Crops. Madison, P53-70.
    29. Peech, M. (1965). Methods of soil analysis Chemical and microbial properties. Madison, P 914–926.
    30. Perret, X., Staehelin, C., Broughton, W. (2000). Molecular basis of symbiotic promiscuity. Mol. Biol., 64: 180-201.
    31. Singh, A., Bhatt, B. (2012). Faba bean: unique germplasm explored and identified. Hort. Flora Res. Spectrum. Plant Soil., 1 :267-269
    32. Somasegaran, P. and Hoben, H. J. (1994). Collecting nodules and isolating rhizobia in : Handbook for Rhizobia, Methods in Legume-    Rhizobium Technology, Springer. P 7-23.
    33. Vered, Y., Grosskopf, I., Palevitch, D., Harsat, A., Charach, G., Weintraub, M., Graff, E. (1997). The influence of Vicia faba (broad bean) seedlings on urinary sodium excretion. Planta Med., 63 :237-40.
    34. Vincent, J. (1970). A manual for the practical study of root-nodule bacterial. IPB handbook, Oxford: P 64.
    35. Walkley, A., Black, (1934). A. An examination of the Degtjareff methods for determining soil organic matter and a proposed modification of the chromic and titration method. Soil Sci., 37:29–38. 
    36. Zohary, D., Hopf, M. (1973). Domestication of pulses in the old world. Science., 182 :887-894 
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