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Legume Research

Ems and gamma radiation induced mutation in grasspea (Lathyrus sativus L.)

DOI: 10.18805/LR-3981    | Article Id: LR-3981 | Page : 300-307
Citation :- Ems and gamma radiation induced mutation in grasspea (Lathyrus sativus L.).Legume Research.2019.(42):300-307
Prabhat K. Singh and R. Sadhukhan singhpk.gpb@gmail.com
Address : Department of Genetics and Plant Breeding, Bidhan Chandra Krishi Vishwavidyalaya, Mohanpur, Nadia- 741 252, West Bengal, India.
Submitted Date : 30-12-2017
Accepted Date : 26-03-2018
First Online:

Abstract

Present investigation has been undertaken to study the induction of mutation by individual and combination treatments of EMS and gamma rays in three diverged genotypes of grasspea viz. Nirmal, Biol-212 and Berhampur Local. Combination treatments caused the more biological damage (lethality, injury and sterility) followed by gamma rays and EMS alone. The LD50 dose of gamma rays for Nirmal, Biol-212 and Berhampur Local corresponded to 590 Gy, 630 Gy and 539 Gy respectively. Above 0.5% EMS for Nirmal and Biol-212 and above 1% EMS in case of Berhampur Local was found to be lethal. Average total mutation frequency of three varieties together was highest (6.32 %) in gamma radiation followed by both EMS and combination treatment (5.8%). The most efficient dose/concentration of gamma rays and EMS were found to be 400 Gy and 0.5% EMS respectively. Mutagenic efficiency increases and effectiveness decreases with increasing concentration of EMS but the consequence was showed almost inverse relationship in case of gamma rays. Overall, irrespective of genotypes, gamma rays and EMS respectively were found to be most efficient and effective mutagen.

Keywords

Effectiveness Efficiency EMS Gamma rays Grasspea LD50

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