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Research Article

volume 42 issue 6 (december 2019) : 729-735,   Doi: 10.18805/LR-3935

Evaluation and molecular characterization of advanced interspecific lines for genetic improvement in mungbean [Vigna radiata (L.) Wilczek]

S
Simranjeet Kaur
T
T.S. Bains
A
Asmita Sirari
S
Satinder Kaur
1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana- 141 004, Punjab, India

  • Submitted01-09-2017|

  • Accepted28-11-2017|

  • First Online 15-02-2018|

  • doi 10.18805/LR-3935

Cite article:- Kaur Simranjeet, Bains T.S., Sirari Asmita, Kaur Satinder (2018). Evaluation and molecular characterization of advanced interspecific lines for genetic improvement in mungbean [Vigna radiata (L.) Wilczek]. Legume Research. 42(6): 729-735. doi: 10.18805/LR-3935.

ABSTRACT

The present study was undertaken to evaluate advanced interspecific lines of mungbean [Vigna radiata (L.) Wilczek] for yield and its components and to understand genetic diversity among these lines using microsatellite markers (SSRs). The experimental material consisted of 51 advanced interspecific lines developed from mungbean [Vigna radiata (L.) Wilczek] ×urdbean [Vigna mungo L. Hepper]  and mungbean [Vigna radiata (L.) Wilczek] ×ricebean [Vigna umbellata Thumb.] crosses and 13 parental lines (mungbean, urdbean and ricebean). A wide range of variability was observed for eleven yield and its component characters and many superior lines were identified. SSR markers synthesised from different Vigna species were used to assess genetic diversity in advanced interspecific lines along with their parents. The interspecific lines were converged into three main clusters while the parental lines were converged into three main and four sub clusters, depicting genetic diversity among them.

REFERENCES

  1. Dikshit H K, Shrama T R, Singh B B and Kumari J (2009) Molecular and morphological characterization of fixed lines from diverse cross in Mungbean [Vigna radaita (L.) Wilczek]. J Genetics 88: 3.
  2. Doyle J J and Doyle J L (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15.
  3. Pandiyan M, Ramamoorthi N, Ganesh S K, Jebaraj S, Pagarhjan P and Balasubramanian P (2008) Broadening the genetic base and introgression of MYMV resistance and yield improvement through unexplored genes from wild relatives in mungbean. Pl Mutation Reports 2(1): 33-38. 
  4. Pandiyan M, Senthil N, Ramamoorthi N, Mithiah A R, Tomooka N, Nuncan V and Jayaraj T (2010) ‘Interspecific hybridization in Vigna radiata x 13 wild Vigna species for developing MYMV donor. Electronic J Pl Breeding 1: 600-10.
  5. Pandiyan M, Senthil N, Suresh R, Chakravarthy N, Packiaraj D and Jagdeep S (2012) Interspecific hybridization of Vigna radiata x Vigna trilobata. Wudpacker J Agric Res 1(6): 233-34.
  6. Perrier X and Jacquemond-Collet J P (2006) DARWIN software. http:# Darwin. cirad.fr/Darwin/Singh B B and Dikshit H K (2002) Possibilities and limitations of interspecific hybridization involving green gram (Phaseolus radiatus) and black gram (Phaseolus mungo). Indian J Agric Sci 72: 676-78. 
  7. Singh S and Bains T S (2006) Wide hybridization in Vigna. In: Advances in Mungbean and Urdbean. [Eds: Masood Ali and Shiv Kumar], IIPR, Kanpur 1: 169-86.
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    Research Article

    volume 42 issue 6 (december 2019) : 729-735,   Doi: 10.18805/LR-3935

    Evaluation and molecular characterization of advanced interspecific lines for genetic improvement in mungbean [Vigna radiata (L.) Wilczek]

    S
    Simranjeet Kaur
    T
    T.S. Bains
    A
    Asmita Sirari
    S
    Satinder Kaur
    1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana- 141 004, Punjab, India

    • Submitted01-09-2017|

    • Accepted28-11-2017|

    • First Online 15-02-2018|

    • doi 10.18805/LR-3935

    Cite article:- Kaur Simranjeet, Bains T.S., Sirari Asmita, Kaur Satinder (2018). Evaluation and molecular characterization of advanced interspecific lines for genetic improvement in mungbean [Vigna radiata (L.) Wilczek]. Legume Research. 42(6): 729-735. doi: 10.18805/LR-3935.

    ABSTRACT

    The present study was undertaken to evaluate advanced interspecific lines of mungbean [Vigna radiata (L.) Wilczek] for yield and its components and to understand genetic diversity among these lines using microsatellite markers (SSRs). The experimental material consisted of 51 advanced interspecific lines developed from mungbean [Vigna radiata (L.) Wilczek] ×urdbean [Vigna mungo L. Hepper]  and mungbean [Vigna radiata (L.) Wilczek] ×ricebean [Vigna umbellata Thumb.] crosses and 13 parental lines (mungbean, urdbean and ricebean). A wide range of variability was observed for eleven yield and its component characters and many superior lines were identified. SSR markers synthesised from different Vigna species were used to assess genetic diversity in advanced interspecific lines along with their parents. The interspecific lines were converged into three main clusters while the parental lines were converged into three main and four sub clusters, depicting genetic diversity among them.

    REFERENCES

    1. Dikshit H K, Shrama T R, Singh B B and Kumari J (2009) Molecular and morphological characterization of fixed lines from diverse cross in Mungbean [Vigna radaita (L.) Wilczek]. J Genetics 88: 3.
    2. Doyle J J and Doyle J L (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15.
    3. Pandiyan M, Ramamoorthi N, Ganesh S K, Jebaraj S, Pagarhjan P and Balasubramanian P (2008) Broadening the genetic base and introgression of MYMV resistance and yield improvement through unexplored genes from wild relatives in mungbean. Pl Mutation Reports 2(1): 33-38. 
    4. Pandiyan M, Senthil N, Ramamoorthi N, Mithiah A R, Tomooka N, Nuncan V and Jayaraj T (2010) ‘Interspecific hybridization in Vigna radiata x 13 wild Vigna species for developing MYMV donor. Electronic J Pl Breeding 1: 600-10.
    5. Pandiyan M, Senthil N, Suresh R, Chakravarthy N, Packiaraj D and Jagdeep S (2012) Interspecific hybridization of Vigna radiata x Vigna trilobata. Wudpacker J Agric Res 1(6): 233-34.
    6. Perrier X and Jacquemond-Collet J P (2006) DARWIN software. http:# Darwin. cirad.fr/Darwin/Singh B B and Dikshit H K (2002) Possibilities and limitations of interspecific hybridization involving green gram (Phaseolus radiatus) and black gram (Phaseolus mungo). Indian J Agric Sci 72: 676-78. 
    7. Singh S and Bains T S (2006) Wide hybridization in Vigna. In: Advances in Mungbean and Urdbean. [Eds: Masood Ali and Shiv Kumar], IIPR, Kanpur 1: 169-86.
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