Evaluation of groundnut genotypes for phosphorus efficiency through leaf acid phosphatase activity

DOI: 10.18805/LR-3927    | Article Id: LR-3927 | Page : 736-742
Citation :- Evaluation of groundnut genotypes for phosphorus efficiency through leaf acid phosphatase activity.Legume Research.2019.(42):736-742
K.V. Naga Madhuri, P. Latha, R.P. Vasanthi, K. John, P.V.R.M. Reddy, G. Murali, T. Giridhara Krishna, T.C.M. Naidu and N.V. Naidu nagamadhurikv@gmail.com
Address : Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N.G Ranga Agricultural University, Tirupati- 517, 502 Andhra Pradesh, India.
Submitted Date : 11-08-2017
Accepted Date : 27-02-2018


Phosphorus (P) sources are limited around the globe making it unsustainable with present farming practices. This necessitates the development of more phosphorus efficient crops while monitoring P status. Groundnut (Arachis hypogaea L.) is a major crop in Southern Andhra Pradesh, India where soils are light textured and less fertile. Twenty groundnut genotypes were evaluated under a pot culture experiment conducted at Regional Agricultural Research Station, Tirupati, Andhra Pradesh, India during kharif, 2016 grown using standard crop recommended practices in P-sufficient (control) and in P-deficient conditions. Leaf Acid phosphatase activity and leaf P content were monitored after 60 days of sowing while kernel P and pod yields were recorded at harvest and estimated using standard protocols. Results showed that under P deficient conditions, genotypes TCGS 1616, TCGS 1622, TCGS 1624, TCGS 1517 and Greeshma had higher leaf P accumulation (between 0.20 % to 0.24 %) in comparison to others. Genotypes TCGS 1616, TCGS 1624, and TCGS 1517 reported high kernel P when compared to others under P starved conditions. Likewise under deficient soil P conditions, TCGS 1528, TCGS 1624, TCGS 1517 and Greeshma (range 2.71-4.45 µmoles hr-1g-1) demonstrated having lower leaf acid phosphatase content in leaves when compared to other genotypes. Only TCGS 1624, TCGS 1616 and Greeshma had lower leaf acid phosphatase while at the same time having higher accumulation of leaf P and highest pod yield followed by TCGS 1517 suggesting that P starvation was better coped by these genotypes and hence can be recommended in soils deficient in P. Leaf acid phosphatase activity correlated negatively with P content in leaves (r = -0.73) similar to those reported for soybean cultivars in Brazil. There is potential for breeders in accelerating identification of markers to specific traits that would be suitable for monitoring P status and manage nutrient application.


Genotypes P-efficiency P-starvation Peanut Phosphorus nutrition.


  1. Ajay, B. C., Singh, A. L., Narendra, K., Dagla M. C., Bera, S. K., Abdul Fiyaz, R. (2015). Role of phosphorus efficient genotypes in increasing crop production. In: Recent Advances in Crop Physiology (ed. A. L. Singh), Astral International, New Delhi, Vol.2 pp. 19-50.
  2. Ascencio, J. (1994). Acid phosphatase as a diagnostic tool. Communications in Soil Science & Plant Analysis, 25: 1553-1564.
  3. Besford, R. (1979a). Phosphorus nutrition and acid phosphatase activity in the leaves of seven plant species. Journal of the Science of Food and Agriculture, 30: 281-285.
  4. Besford, R.T. (1979b). Nutrient imbalances in tomato plants and acid phosphatase activity in the leaves. Journal of the Science of Food and Agriculture, 30: 275-280. 
  5. Boutin, J.P., Provot, M., Roux, L. (1981). Effect of cycloheximide and renewal of phosphorus supply on surface acid phosphatase activity of phosphorus deficient tomato roots. Physiologia plantarum, 51: 353-360.
  6. Breseghlo, M.L., Oliveira, I.P., Thung, M.D.T. (1992). Resposta de cultivares de feijão ao teste de fosfatase ácida.Pesquisa Agropecuária Brasileira, 27: 647-654. 
  7. Duff, S.M., Sarath, G. and Plaxton, W.C. (1994). The role of acid phosphatases in plant phosphorus metabolism. Physiologia Plantarum, 90: 791-800.
  8. Föshe, D., Claassen, N., Junk, A. (1991). Phosphorus efficiency of plants: II. Significance of root radius, root hairs and catio-anion balance for phosphorus influx in seven plant species. Plant and Soil, 132: 261-272. 
  9. Gabelman, W.H. and Gerloff, G.C. (1983). The search for and interpretation of genetic controls that enhance plant growth under    deficiency levels of a macronutrient. Plant and Soil, 72(2): 335-350. 
  10. Gourley, C.J.P., Allan, D.L. Russelle, M.P. (1993). Defining phosphorus efficiency in plants. Plant and Soil, 155(1): 289-292.
  11. Guthrie, R.E., McLachlan, K.D., Marco, D.G.D. (1991). Acid phosphatases associated with phosphorus deficiency in wheat: partial purification and properties. Functional Plant Biology, 18: 615-626.
  12. Hammond J.P., Broadley M.R., White P.J. (2004). Genetic responses to phosphorus deficiency. Annals of Botany, 94: 223-332.
  13. Jadhav, S.S. and Gowda, M.V.C. (2012). Assessment of genetic variation and selection of most responsive lines for root traits in relation to phosphorous nutrition in groundnut (Arachis hypogaea L.). Indian Journal of Genetics and Plant Breeding, 72(4): 439-444.
  14. Krishna, K.R. (1997). Phosphorus uptake and utilization efficiency in peanut. Peanut Science, 24: 1-6.
  15. Kumar S., Kollalu S., Murali M. (2014). Nutrient use efficiency of groundnut as influence by nitrogen and phosphorus levels in northern transition zone of Karnataka. Trends in Bioscience, 7: 3461-3464
  16. Kumar A., Pandey A., Gowda M.V.C. (2015). Evaluation of groundnut genotypes for tolerance to phosphorus deficiency.Journal of Plant Nutrition, 38 687-699.
  17. Li, Y.F., Luo, A.C., Wei, X.H., Chaudry, A.K. (2009). Relationship between leaf acid phosphatase activity and either P nutritional status or P efficiency in rice. Pak J Bot, 41: 109-119.
  18. Naga Madhuri K.V., Latha P, Vasanthi RP, John K, Reddy, P.V.R.M., Lavanya Kumari P., et al (2017). Evaluation of groundnut genotypes for phosphorus efficiency through leaf acid phosphatase activity. Proceedings of International onference Inter Drought V, Hyderabad from 21-25 Feb., 2017.
  19. Ortiz N.C. (2006). Phosphorus efficiency of Arachis pintoi genotypes and possible mechanisms for tolerance to low soil Psupply. PhD Thesis. Georg-August-University of Gottingen.
  20. Pandey., M.K., Verma, A., Pramod, K. (2016). Effect of integrated phosphorus management on growth, yield and quality of lentil (Lens culinaris). Indian Journal of Agricultural Research. 50: 238-243.
  21. Raghothama K. G., (1999). Phosphate acquisition. Ann. Rev. Plant Physiol. Plant Mol. Biol., 50: 665-693.
  22. Raposo R.W.C., Muraoka T., Basso L.C., Lavres Jr J., Franzini V.I. (2004). Acid phosphatase activity and leaf phosphorus content in soybean cultivars. Sci. Agric. (Piracicaba, Braz), 61: 439-445.
  23. Rashmi., I., Biswas, A.K., Ramakrishna P.V.R. (2014). Phosphorus management in agriculture: a review. Agri. Reviews.35: 261-270.
  24. Singh, K., Manohar, R.S., Choudhary, R., Yadav, A.K., Sangwan, A. (2015a). Response of different sources and levels of phosphorus on yield, nutrient uptake and net returns on mungbean under rainfed conditions. Indian Journal of Agricultural Research. 35: 263-268.
  25. Singh, A.L., Chaudhari, V., Ajay B.C., (2015b). Screening of groundnut genotypes for phosphorus efficiency under field conditions. Indian Journal of Genetics, 75: 363-371.
  26. Tandon H.L.S. (1993). Methods of Analysis of Soils, Plants, Waters and Fertilizers. Ed. Fertilizers Development and Consultation Organisation, New Delhi, India.
  27. Trull, M.C., Guiltinan, M.J., Lynch, J.P., Deikman J. (1997). The responses of wild-type and ABA mutant Arabidopsis thaliana plants to phosphorus starvation. Plant Cell and Environment, 20: 85-92.
  28. Vance, C.P., Uhde-Stone, C., Allan D.L. (2003). Phosphorus acquisition and use: critical adaptations by plants for securing a non-    renewable resource. New Phytologist, 157: 423-447.
  29. Wang, X., Yan X., Liao, H. (2010). Genetic improvement for phosphorus efficiency in soybean: a radical approach. Annals of Botany, 106: 215-222.
  30. Zogli, P., Pingault, L., Libault, M. (2017). Physiological and Molecular Mechanisms and Adaptation Strategies in Soybean (Glycine max) Under Phosphate Deficiency. Legume Nitrogen fixation in soils with low phosphorus availability.Pp 219-242. 

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