Banner

Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.32, CiteScore: 0.906

  • Impact Factor 0.8, Q3

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Legume Research, volume 42 issue 5 (october 2019) : 710-714

Molecular tagging of botrytis grey mould disease in chickpea

Ranjana, Anju Arora, R.K. Panwar, S.K. Verma
1Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture and Technology, Pantangar-263 145, Uttarakhand, India.

  • Submitted11-08-2017|

  • Accepted09-05-2018|

  • First Online 16-10-2018|

  • doi 10.18805/LR-3925

Cite article:- Ranjana, Arora Anju, Panwar R.K., Verma S.K. (2018). Molecular tagging of botrytis grey mould disease in chickpea. Legume Research. 42(5): 710-714. doi: 10.18805/LR-3925.

ABSTRACT

Validation of two primers TA144 and TR29 for their linkage with botrytis grey mould resistance gene was studied in twenty genotypes of chickpea including resistant (GL10006) and susceptible (DCP92-3) checks. The genotypes were also scored for disease reaction under natural exposure at hot spot location. Molecular analysis with primers TA144 and TR29 showed polymorphic bands in GL10006 and DCP92-3. Therefore, it can be concluded that the primers are linked with resistance gene. The genotypes that showed amplicons with both the primers at same or nearby positions of resistant check were HK-2, HK-4, BG-1003, KAK-2 and GNG-1969 and indicated as resistant or moderately resistant ones. The above genotypes were also found resistant under natural field condition. Thus both the primers TA144 and TR29 showed good correlation with phenotypic evaluation in most of the genotypes studied. In future, more number of primers nearby the resistance gene(s) can be tried for their contribution towards overall disease reaction.

REFERENCES

  1. Anbessa, Y., Taran, B., Warkentin, T.D., Tullu, A. and Vandenberg, A. (2009). Genetic analyses and conservation of QTL for ascochyta blight resistance in chickpea (Cicer arietinum L.). Theor. Appl. Genet. 119: 757-765.
  2. Chakraborthi, D., Sarkar, A. and Guptha, S. (2006). Small and large scale genomic DNA isolation protocol for chickpea (Cicer arietinum L.) suitable for molecular marker transgenic analysis. African J. Biotech. 5(8): 585-589.
  3. Chetukuri, A., Gaur, P. M., Pande, S., Gali, K. K.; Ganesh, M.,Kumar, J. and Varshney, R. K. (2011). Mapping QTL for resistance to botrytis grey mould in chickpea. Euphytica, 182: 1-9.
  4. Davidson, J. A., Pande, S., Bretag, T. W., Lindbeck, K. D. and Kishore, G. K. (2004). Biology and management of Botrytis spp. in legume crops. In: Botrytis biology, pathology and control. Kluwer Academic Publishers: The Netherlands. pp. 295–318.
  5. Grewal, J. S. and Laha, S. K. (1983). Chemical control of botrytis blight in chickpea. Indian Phytopathol, 36: 516–520.
  6. Kaur, L., Sirari, A., Kumar, D., Sandhu, J.S., Singh, S., Kapoor, K., Singh, I., Gowda, C.L.L., et al. (2013). Combining Ascochyta blight and Botrytis grey mould resistance in chickpea through interspecific hybridization. Phytopathologia Mediterranea. 52(1): 157-165.
  7. Knights, E. J. and Siddique, K. H.M. (2002). Manifestation of Botrytis cinerea on chickpeas in Australia. In: Workshop Proceedings Integrated Management of Botrytis Grey Mould of Chickpea in Bangladesh and Australia, Bangladesh Agricultural Research Institute: Joydebpur, Gazipur, Bangladesh. pp. 70-77.
  8. McIntosh, M. and Miller, C. (2001). A diet containing food rich in soluble and insoluble fiber improves glycemic control and reduces hyperlipidemia among patients with type 2 diabetes mellitus. Nutrition Reviews. 59: 52-5.
  9. Rashid, M.H., Shahadat, M.K., Hasnat, M.R. Kashem, M.A., Rafii, M.Y. and Latif, M.A. (2015). Screening of deshi chickpea (Cicer arietinum L.) germplasm resistant to Botrytis Gray Mold in Bangladesh. Legume Research. 38(6): 861-863. 
  10. Pande, S., Galloway, J., Gaur, P. M., Siddique, K. H. M., Tripathi, H. S., Taylor, P., MacLeod, M. W. J., et al . (2006). Botrytis grey mould of chickpea: a review of biology, epidemiology, and disease management. Australian Journal of Agricultural Research, 57: 1137–1150.
  11. Pande, S., Ramgopal, D., Kishore, G. K., Mallikarjuna, N., Sharma, M., Pathak, M. and Rao, N. J. et al. (2006). Evaluation of Wild Cicer Species for Resistance to Ascochyta Blight and Botrytis Gray Mold in Controlled Environment at ICRISAT, Patancheru, India. 2(1): 33-35.
  12. Pittaway, J.K. Ahuja, K.D., Cehun, M., Chronopoulos, A., Robertson, I.K., Nestel, P.J. and Ball, M.J. (2006). Dietary supplementation with chickpeas for at least 5 weeks results in small but significant reductions in serum total and low-density lipoprotein cholesterols in adult women and men. Annals of Nutrition and Metabolism. 50: 512-518. 
  13. Winter, P., Pfaff, T., Udupa, S.M., Huettel, B., Sharma, P. C., Sahi, S., Arrequin-Espinoza, R., et al. (1999). Characterization and mapping of sequence- tagged microsatellite sites in the chickpea (Cicer arietinum L.). Mol. Genet. 296: 90-101.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)