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Research Article

volume 41 issue 3 (june 2018) : 374-378,   Doi: 10.18805/LR-3878

Seed morphology, protein profiling and taxonomic relationships among certain Legume-Mimosoid taxa

H
Harikrishna Naik Lavudi
K
Kottapalli Seshagirirao
1Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Central University, Hyderabad-500 046, Telangana, India

  • Submitted14-04-2017|

  • Accepted05-10-2017|

  • First Online 16-05-2018|

  • doi 10.18805/LR-3878

Cite article:- Lavudi Naik Harikrishna, Seshagirirao Kottapalli (2018). Seed morphology, protein profiling and taxonomic relationships among certain Legume-Mimosoid taxa. Legume Research. 41(3): 374-378. doi: 10.18805/LR-3878.

ABSTRACT

Seed morphology and protein profiles were done for nine taxa of Leguminosae subfamily Mimosoideae, namely Acacia farnesiana, Adenanthera pavonina, Albizia lebbeck, A. saman, Dichrostachys cinerea, Entada rheedii, Mimosa pudica, Parkia biglandulosa and Pithecellobium dulce, using Scanning Electron Microscope and SDS-PAGE analysis. The total protein profile (electrophorogram) revealed the presence or absence of protein bands of molecular weights ranging from 11 to 100 kDa. There were considerable variations in band patterns of the taxa investigated. The phenogram has the nine taxa forming four clusters, corresponding to the four tribes of Mimosoideae. The variations in the seed morphology and biochemical aspects of the taxa were of use in the proper identification, classification and to discuss relationships among the nine Mimosoid taxa.

REFERENCES

  1. Ayaz, F.A., Inceer, H. and Hayirlioglu-Ayaz, S. (1999). An electrophoretic analysis of the seed proteins of some Vicia L. species from Northeast Anatolia (Turkey). Pakistan J. Biol. Sci. 2(4): 1139-1142.
  2. Baker, J.G. (1876-1878). Leguminosae, in The Flora of British India 2:56-306, Hooker, J.D. Reeve & Co., London.
  3. Bouchenak-Khelladi, Y., Maurin, O., Hurter, J. and Bank. M.V.D. (2010). The evolutionary history and biogeography of Mimosoideae (Leguminosae): An emphasis on African Acacias. Mol. Phylogenet. Evou. 57: 495-508.
  4. Brown, G.K., Murphy, D.J., Miller, J.T. and Ladiges, P.Y. (2008). Acacia s.s. and its relationships among tropical legumes, tribe Ingeae (Leguminosae: Mimosoideae). Syst. Bot. 33: 739-751.
  5. Carolin, RC. (1980). Pattern of seed surface of Godenia and related genera. Austra. J. Bot. 28: 123-137.
  6. Çelebi, A., Açýk, L. and Aytaç, Z. (2009). Biosystematics studies among Ebenus L. species based on morphological, RAPD-PCR and seed protein analyses in Turkey. Pak. J. Bot., 41(5): 2477-2486.
  7. Chen, L., Fischer, H. and Jensen, U. (1997). Accumulation of seed storage proteins and the taxonomy of poaceae. Plant Syst. Evol. 206: 243-257.
  8. Crepet, W.L. and Taylor, D.W. (1986). Primitive mimosoid flowers from the Paleocene-Eocene and their systematic and evolutionary implications. Am. J. Bot. 73: 548-563.
  9. Dahlgren, R. and Clifford, H.T. (1982). The Monocotyledons. A comparative Study. Acad. Press. London, New York.
  10. Gamble, J.S. and Fischer, C.E.C. (1915-1935). Flora of the Presidency of Madras. 3 Vols. Adlard & Sons Ltd., London.
  11. Ghafoor, A., Z. Ahmad, A.S. Qureshi and Bashir, M. (2002). Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE. Euphytica, 123: 367-378. 
  12. Hameed, A., Shah, T.M., Atta, B.M., Iqbal, N., Haq, M.A. and Ali, H. (2009). Comparative seed storage protein profiling of Kabuli chickpea genotypes. Pak. J. Bot., 41(2): 703-710.
  13. Javaid, A., Ghafoor, A. and Anwar, R. (2004). Seed storage protein electrophoresýs in groundnut for evaluating genetic diversity. Pak. J. Bot., 36(1): 25-29.
  14. Jobson, R.W. and Luckow, M. (2007). Phylogenetic study of the genus Piptadenia (Mimosoideae Leguminosae) using plastid trnL-F and trnK/matK sequence data. Syst. Bot. 32: 569-575.
  15. Kamel, E.A., Hassan, H.Z. and Ahmed, S.M. (2003). Electrophoretic characterization and the relationship between some Egyptian Cruciferae. J. Bol. Sci, 3(9): 834-842.
  16. Khurshid, H. and Rabbani, M.A. (2012). Comparison of eelectrophoretic protein profiles from seed of different oilseed Brassica cultivars. J. Pub. Health and Bio. Sc., 1: 36-42.
  17. Ladizinsky, G. and Hymowitz, T. (1979). Seed Protein Electrophoresis in Taxonomic and Evolutionary Studies. Theor. Appl. Genet., 51: 145-151.
  18. Ladizinsky, G., and Van Oss, H. (1984). Genetic Relationships Between Wild and Cultivated Vicia ervilia L. Bot. J. of Linn. Soc., 89: 97-100.
  19. Lavin, M. and Matos, A.B. (2008). The impact of ecology and biogeography on legume diversity, endemism, and phylogeny in the Caribbean region: a new direction in historical biogeography. Bot. Rev. 74: 178-196.
  20. Lavin, M., Herendeen, P.S. and Wojciechowski, M.F. (2005). Evolutionary rates analysis of Leguminosae implicates a rapid diversification of lineages during the tertiary. Syst. Biol. 54: 575-594.
  21. Lewis, G.P., Schrire, B., MacKinder, B. and Lock, M. (2005). Legumes of the World. Royal Botanic Gardens, Kew, UK.
  22. Linskens, HF. Pfahler, PL. Knuiman, EL. (1977). Identification of soybean cultivars by the surface relief of the seed-coat. Theore. Appl. Genet., 50: 147-150.
  23. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). Protein measerment with the Folin phenol reagent. J. Biol. Chem. 193: 265-271.
  24. Luckow, M., Fortunato, R.H., Sede, S. and Livshultz, T. (2005). The phylogenetic affinities of two mysterious monotypic mimosoids from Southern South America. Syst. Bot. 30: 585-602.
  25. Luckow, M., Miller, J.T., Murphy, D.J. and Livshultz, T. (2003). A phylogenetic analysis of the Mimosoideae (Leguminosae) based on chloroplast DNA sequence data. pp. 197-220. In: Adv. in Legumes Syst. [Klitgaard, B.B., Bruneau, A. (eds.)], Royal Botanic Gardens, Kew, UK.
  26. Mabberley, D. J. (1997). The Plant Book, ed. 2. - Cambridge.
  27. Miller, J.T. (2008). A review of the classification of Acacia (Leguminosae, Mimosoideae). National Herbarium of Victoria, Royal Botanic Gardens Melbourne, Birdwood Avenue, South Yarra, Victoria 3141, Muelleria, 26(1): 10–26.
  28. Miller, J.T., Grimes, J.W., Murphy, D.J., Bayer, R.J. and Ladiges, P.Y. (2003). A phylogenetic analysis of the Acacieae and Ingeae (Mimosoideae: Fabaceae) based on trnK, matK, psbA-trnH, and trnL/trnF sequence data. Syst. Bot. 28(3): 558-566.
  29. Pfosser, M., Wetschnig, W., Unger, S. and Prenner, G. (2003). Phylogenetic relationships among genera of Massonieae(Hyacinthaceae) inferred from plastid DNA and seed morphology. J. Plant Res. 116: 115-132.
  30. Polhill, R. M., Raven, P. H. and Stirton, C. H. (1981). Advances in Legume Systematics, 1: 1-26.
  31. Rao, R.R. (2002). Advances in legume Research in India, B. Singh & M.P. Singh publishers, Dehra Dun, India.
  32. Rohlf, F.J. (1993). NTSYS-PC Version 2.0. State University of New York, Exeter Software, Setauket: New York.
  33. Sanjappa, M. (1992). Legumes of India. Bishen Singh &M.P. Singh Publishers, Dehra Dun, India.
  34. Sanjeev, R., Rajesh, M., Madhuri, B. and Narendra, B.P.V.R. (2012). Comparative evolution of protein extraction methods from few Leguminous seeds. Int. J. of Adv. Biotech Res. 3(2): 558-563.
  35. Trivedi, B.S., Bagchi, G.D. and Bajpai, U. (1980). Studies on seeds and spermoderm structure of Bauhinia. Phytomorph., pp11-16.
  36. Valizadeh, M. (2001). Seed storage protein profile of grain legumes grown in iran, Using SDS-PAGE, J. Agric. Sci. Technol. 3: 287-292.
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    Research Article

    volume 41 issue 3 (june 2018) : 374-378,   Doi: 10.18805/LR-3878

    Seed morphology, protein profiling and taxonomic relationships among certain Legume-Mimosoid taxa

    H
    Harikrishna Naik Lavudi
    K
    Kottapalli Seshagirirao
    1Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Central University, Hyderabad-500 046, Telangana, India

    • Submitted14-04-2017|

    • Accepted05-10-2017|

    • First Online 16-05-2018|

    • doi 10.18805/LR-3878

    Cite article:- Lavudi Naik Harikrishna, Seshagirirao Kottapalli (2018). Seed morphology, protein profiling and taxonomic relationships among certain Legume-Mimosoid taxa. Legume Research. 41(3): 374-378. doi: 10.18805/LR-3878.

    ABSTRACT

    Seed morphology and protein profiles were done for nine taxa of Leguminosae subfamily Mimosoideae, namely Acacia farnesiana, Adenanthera pavonina, Albizia lebbeck, A. saman, Dichrostachys cinerea, Entada rheedii, Mimosa pudica, Parkia biglandulosa and Pithecellobium dulce, using Scanning Electron Microscope and SDS-PAGE analysis. The total protein profile (electrophorogram) revealed the presence or absence of protein bands of molecular weights ranging from 11 to 100 kDa. There were considerable variations in band patterns of the taxa investigated. The phenogram has the nine taxa forming four clusters, corresponding to the four tribes of Mimosoideae. The variations in the seed morphology and biochemical aspects of the taxa were of use in the proper identification, classification and to discuss relationships among the nine Mimosoid taxa.

    REFERENCES

    1. Ayaz, F.A., Inceer, H. and Hayirlioglu-Ayaz, S. (1999). An electrophoretic analysis of the seed proteins of some Vicia L. species from Northeast Anatolia (Turkey). Pakistan J. Biol. Sci. 2(4): 1139-1142.
    2. Baker, J.G. (1876-1878). Leguminosae, in The Flora of British India 2:56-306, Hooker, J.D. Reeve & Co., London.
    3. Bouchenak-Khelladi, Y., Maurin, O., Hurter, J. and Bank. M.V.D. (2010). The evolutionary history and biogeography of Mimosoideae (Leguminosae): An emphasis on African Acacias. Mol. Phylogenet. Evou. 57: 495-508.
    4. Brown, G.K., Murphy, D.J., Miller, J.T. and Ladiges, P.Y. (2008). Acacia s.s. and its relationships among tropical legumes, tribe Ingeae (Leguminosae: Mimosoideae). Syst. Bot. 33: 739-751.
    5. Carolin, RC. (1980). Pattern of seed surface of Godenia and related genera. Austra. J. Bot. 28: 123-137.
    6. Çelebi, A., Açýk, L. and Aytaç, Z. (2009). Biosystematics studies among Ebenus L. species based on morphological, RAPD-PCR and seed protein analyses in Turkey. Pak. J. Bot., 41(5): 2477-2486.
    7. Chen, L., Fischer, H. and Jensen, U. (1997). Accumulation of seed storage proteins and the taxonomy of poaceae. Plant Syst. Evol. 206: 243-257.
    8. Crepet, W.L. and Taylor, D.W. (1986). Primitive mimosoid flowers from the Paleocene-Eocene and their systematic and evolutionary implications. Am. J. Bot. 73: 548-563.
    9. Dahlgren, R. and Clifford, H.T. (1982). The Monocotyledons. A comparative Study. Acad. Press. London, New York.
    10. Gamble, J.S. and Fischer, C.E.C. (1915-1935). Flora of the Presidency of Madras. 3 Vols. Adlard & Sons Ltd., London.
    11. Ghafoor, A., Z. Ahmad, A.S. Qureshi and Bashir, M. (2002). Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE. Euphytica, 123: 367-378. 
    12. Hameed, A., Shah, T.M., Atta, B.M., Iqbal, N., Haq, M.A. and Ali, H. (2009). Comparative seed storage protein profiling of Kabuli chickpea genotypes. Pak. J. Bot., 41(2): 703-710.
    13. Javaid, A., Ghafoor, A. and Anwar, R. (2004). Seed storage protein electrophoresýs in groundnut for evaluating genetic diversity. Pak. J. Bot., 36(1): 25-29.
    14. Jobson, R.W. and Luckow, M. (2007). Phylogenetic study of the genus Piptadenia (Mimosoideae Leguminosae) using plastid trnL-F and trnK/matK sequence data. Syst. Bot. 32: 569-575.
    15. Kamel, E.A., Hassan, H.Z. and Ahmed, S.M. (2003). Electrophoretic characterization and the relationship between some Egyptian Cruciferae. J. Bol. Sci, 3(9): 834-842.
    16. Khurshid, H. and Rabbani, M.A. (2012). Comparison of eelectrophoretic protein profiles from seed of different oilseed Brassica cultivars. J. Pub. Health and Bio. Sc., 1: 36-42.
    17. Ladizinsky, G. and Hymowitz, T. (1979). Seed Protein Electrophoresis in Taxonomic and Evolutionary Studies. Theor. Appl. Genet., 51: 145-151.
    18. Ladizinsky, G., and Van Oss, H. (1984). Genetic Relationships Between Wild and Cultivated Vicia ervilia L. Bot. J. of Linn. Soc., 89: 97-100.
    19. Lavin, M. and Matos, A.B. (2008). The impact of ecology and biogeography on legume diversity, endemism, and phylogeny in the Caribbean region: a new direction in historical biogeography. Bot. Rev. 74: 178-196.
    20. Lavin, M., Herendeen, P.S. and Wojciechowski, M.F. (2005). Evolutionary rates analysis of Leguminosae implicates a rapid diversification of lineages during the tertiary. Syst. Biol. 54: 575-594.
    21. Lewis, G.P., Schrire, B., MacKinder, B. and Lock, M. (2005). Legumes of the World. Royal Botanic Gardens, Kew, UK.
    22. Linskens, HF. Pfahler, PL. Knuiman, EL. (1977). Identification of soybean cultivars by the surface relief of the seed-coat. Theore. Appl. Genet., 50: 147-150.
    23. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). Protein measerment with the Folin phenol reagent. J. Biol. Chem. 193: 265-271.
    24. Luckow, M., Fortunato, R.H., Sede, S. and Livshultz, T. (2005). The phylogenetic affinities of two mysterious monotypic mimosoids from Southern South America. Syst. Bot. 30: 585-602.
    25. Luckow, M., Miller, J.T., Murphy, D.J. and Livshultz, T. (2003). A phylogenetic analysis of the Mimosoideae (Leguminosae) based on chloroplast DNA sequence data. pp. 197-220. In: Adv. in Legumes Syst. [Klitgaard, B.B., Bruneau, A. (eds.)], Royal Botanic Gardens, Kew, UK.
    26. Mabberley, D. J. (1997). The Plant Book, ed. 2. - Cambridge.
    27. Miller, J.T. (2008). A review of the classification of Acacia (Leguminosae, Mimosoideae). National Herbarium of Victoria, Royal Botanic Gardens Melbourne, Birdwood Avenue, South Yarra, Victoria 3141, Muelleria, 26(1): 10–26.
    28. Miller, J.T., Grimes, J.W., Murphy, D.J., Bayer, R.J. and Ladiges, P.Y. (2003). A phylogenetic analysis of the Acacieae and Ingeae (Mimosoideae: Fabaceae) based on trnK, matK, psbA-trnH, and trnL/trnF sequence data. Syst. Bot. 28(3): 558-566.
    29. Pfosser, M., Wetschnig, W., Unger, S. and Prenner, G. (2003). Phylogenetic relationships among genera of Massonieae(Hyacinthaceae) inferred from plastid DNA and seed morphology. J. Plant Res. 116: 115-132.
    30. Polhill, R. M., Raven, P. H. and Stirton, C. H. (1981). Advances in Legume Systematics, 1: 1-26.
    31. Rao, R.R. (2002). Advances in legume Research in India, B. Singh & M.P. Singh publishers, Dehra Dun, India.
    32. Rohlf, F.J. (1993). NTSYS-PC Version 2.0. State University of New York, Exeter Software, Setauket: New York.
    33. Sanjappa, M. (1992). Legumes of India. Bishen Singh &M.P. Singh Publishers, Dehra Dun, India.
    34. Sanjeev, R., Rajesh, M., Madhuri, B. and Narendra, B.P.V.R. (2012). Comparative evolution of protein extraction methods from few Leguminous seeds. Int. J. of Adv. Biotech Res. 3(2): 558-563.
    35. Trivedi, B.S., Bagchi, G.D. and Bajpai, U. (1980). Studies on seeds and spermoderm structure of Bauhinia. Phytomorph., pp11-16.
    36. Valizadeh, M. (2001). Seed storage protein profile of grain legumes grown in iran, Using SDS-PAGE, J. Agric. Sci. Technol. 3: 287-292.
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