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Research Article

volume 41 issue 4 (august 2018) : 526-531,   Doi: 10.18805/LR-3808

Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek
 

P
Prince Lekhi
R
R.K. Gill
S
Satinder Kaur
T
T.S. Bains
1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 004, Punjab, India

  • Submitted03-12-2016|

  • Accepted15-06-2017|

  • First Online 04-04-2018|

  • doi 10.18805/LR-3808

Cite article:- Lekhi Prince, Gill R.K., Kaur Satinder, Bains T.S. (2018). Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek. Legume Research. 41(4): 526-531. doi: 10.18805/LR-3808.

ABSTRACT

Vigna radiata genotypes viz., SML 668 and SML 832 and V. mungo genotypes viz., Mash 114 and Mash 218 were crossed in all possible combinations during summer 2015 to generate F1 hybrids. Interspecific hybridization was attempted by using V. radiata genotypes as female parent. Pod set percentage varied from 5.5 percent (SML 832 x Mash 218) to 24.1 percent (SML 832 x Mash 114). The germination percentage ranged from 14.29 to 30.56. Maximum pollen fertility was observed in cross SML 668 x Mash 114 (28.36 percent) followed by SML 668 x Mash 218 (27.03 percent), SML 832 x Mash 218 (24.32 percent) and minimum in SML 832 x Mash 114 (22.59 percent).The purity of hybrids were tested through microsatellite markers. For parental polymorphism, microsatellite markers were selected from related Vigna species such as Vigna unguiculata, Vigna radiata and Vigna mungo. Out of 84 markers used, 46 were polymorphic i.e 54.76 per cent polymorphism between parents. These polymorphic markers were used for confirmation of hybrids produced from different crosses. All the F1 plants gave resistant reaction to Mungbean yellow mosaic virus (MYMV) indicating the introgression of resistance gene(s) from V. mungo to V. radiata.

REFERENCES

  1. Anonymous. (2015-16). Project Coordinators Report, All India Coordinated Research Project on MULLARP (ICAR), Indian Institute of Pulses Research, Kanpur: 26.
  2. Bharathi, A., Selvaraj, K. S., Veerabadhiran, P. and Lakshmi, B. S. (2006). Crossability barriers in mungbean [Vigna radiata (L.) Wilczek) with its wild relatives. Indian J. Crop Sci. 1: 120-124.
  3. Boiling, M., Sander, D. A. and Matlock, R. S. (1961). Mungbean hybridization technique. Agron. J. 53: 54-55.
  4. Biswas, M. R. and Dana, S. (1976). Allopolyploid of Tetraploid Phaseolus species × P. calcaratus cross. Biologia Plantarum 18: 335-338.
  5. Doyle, J. J. and Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15.
  6. Dikshit, H. K., Singh, D., Singh, A., Jain, N., Kumari, J. and Sharma, T. R. (2012). Utility of adzuki bean [Vignaangularis (Willd.) Ohwi and Ohashi] simple sequence repeat (SSR) markers in genetic analysis of mungbean and related Vigna spp. Afri. J.Biotechnol. 11: 13261-13268.
  7. Gosal, S. S. and Bajaj, Y. P. S. (1983). Interspecific hybridization between Vigna mungo and Vigna radiata through embryo culture. Euphytica 32: 129-137.
  8. Nene, Y. L., Srivastava, S. K. and Naresh, J. S. (1972). Evaluation of urdbean (Phaseolus mungo L.) and mungbean (Phaseolus aureus L.) germplasms and cultivars against yellow mosaic virus.Indian J. Agric. Sci. 42: 251-254.
  9. Pal, S. S., Singh, J. J. and Singh, I. (2000). Transfer of YMV resistance in cultivar SML 32 of Vigna radiata from other related Vigna species. Pl. Disease Res. 15: 67-69.
  10. Pandiyan, M., Senthil, N., Ramamoorthi, N., Mithiah, A. R., Tomooka, N., Duncan, V. and Jayaraj, T. (2010) Interspecific hybridization of Vigna radiata x 13 wild Vigna species for developing MYMV donar. Electr. J. Plant Breed. 1: 600-610.
  11. Pandiyan, M., Senthil, N., Suresh, R., Chakravarthy, N., Packiaraj, D. and Jagdeep, S. (2012). Interspecific hybridization of Vigna radiata x Vigna trilobata. Wudpacker J. Agric. Res. 1: 233-34.
  12. Rashid, K. A., Smartt, J. and Haq, N. (1987). Hybridization in the genus Vigna. In: Proc. 2nd Int. Symp. (Ed. S. Shanmugasundram and B. T. Mclean).AVRDC.Shanhua, Taiwan. pp.205-214
  13. Satija, C. K. and Vikal, Y. (1993). Crossability and morphological studies among wild and cultivated species of Vigna. J. Cytol. Genet. 28: 173-179.
  14. Sehrawat, N., Yadav, M., Bhat, K. V., Sairam, R. K., and Jaiwal, P. K. (2016) Introgression of mungbean yellow mosaic virus resistance in Vigna mungo(L.) Hepper and purity testing of F1 hybrids using SSRs. Turkish J. Agri. and Forestry 40: 95-100.
  15. Shanmugam, A. S., Rathnaswamy, R. and Rangaswamy, S. R. (1983) Crossability studies between greengram and blackgram. Curr. Sci. 52: 1018-20.
  16. Shukla, G. P., Pandya, B. P. and Singh. D. P. (1978). Inheritance of resistance to yellow mosaic in mungbean. Indian J. Genet. 38: 358-360.
  17. Singh, D. P. (1990).Distant hybridization in genus Vigna–a review.Indian J. Genet.Plt. Breed. 50: 268-276.
  18. Sehrawat, N., Bhat, K. V., Kaga, A., Tomooka, N., Yadav, M. and Jaiwal, P. K. (2014). Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L.Wilczek). Span. J. Agric. Res. 12: 732-741.
  19. Somta, P., Kaga, A., Tomooka, N., Kashiwaba, K., Isemura, T.,Chaitieng, B., Srinives, P. and Vaughan, D. A. (2006). Development of an interspecific Vigna linkage map between Vigna umbellata (Thunb.) Ohwi & Ohashi and V. nakashimae (Ohwi) Ohwi & Ohashi and its use in analysis of bruchid resistance and comparative genomics. Pl. Breed. 125: 77-84.
  20. Subramanian, D. (1980). Interspecific hybridization in Vigna. Indian J. Genet. Plt. Breed. 40: 437-38.
  21. Tabbasam, N., Rahman, M. and Zafar, Y. (2006). DNA-based genotyping of sorghum hybrids.Pak. J.Bot. 38: 1599-1604.
  22. Tangphatsornruang, S., Somta, P., Uthaipaisanwong, P., Chanprasert, J., Sangsrakru, D., Seehalak, W., Sommanas, W. (2009). Characterization of microsatellites and gene contents from genome shotgun sequences of mungbean [Vigna radiata (L.) Wilczek] BMC Pl. Bio. 9: 137.
  23. Wu, M. F. (2001) Expression profile analysis of Hsc70 of Vigna radiata; Master’s Thesis. National TsinHua University, Hsinchu 
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    Research Article

    volume 41 issue 4 (august 2018) : 526-531,   Doi: 10.18805/LR-3808

    Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek
     

    P
    Prince Lekhi
    R
    R.K. Gill
    S
    Satinder Kaur
    T
    T.S. Bains
    1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 004, Punjab, India

    • Submitted03-12-2016|

    • Accepted15-06-2017|

    • First Online 04-04-2018|

    • doi 10.18805/LR-3808

    Cite article:- Lekhi Prince, Gill R.K., Kaur Satinder, Bains T.S. (2018). Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek. Legume Research. 41(4): 526-531. doi: 10.18805/LR-3808.

    ABSTRACT

    Vigna radiata genotypes viz., SML 668 and SML 832 and V. mungo genotypes viz., Mash 114 and Mash 218 were crossed in all possible combinations during summer 2015 to generate F1 hybrids. Interspecific hybridization was attempted by using V. radiata genotypes as female parent. Pod set percentage varied from 5.5 percent (SML 832 x Mash 218) to 24.1 percent (SML 832 x Mash 114). The germination percentage ranged from 14.29 to 30.56. Maximum pollen fertility was observed in cross SML 668 x Mash 114 (28.36 percent) followed by SML 668 x Mash 218 (27.03 percent), SML 832 x Mash 218 (24.32 percent) and minimum in SML 832 x Mash 114 (22.59 percent).The purity of hybrids were tested through microsatellite markers. For parental polymorphism, microsatellite markers were selected from related Vigna species such as Vigna unguiculata, Vigna radiata and Vigna mungo. Out of 84 markers used, 46 were polymorphic i.e 54.76 per cent polymorphism between parents. These polymorphic markers were used for confirmation of hybrids produced from different crosses. All the F1 plants gave resistant reaction to Mungbean yellow mosaic virus (MYMV) indicating the introgression of resistance gene(s) from V. mungo to V. radiata.

    REFERENCES

    1. Anonymous. (2015-16). Project Coordinators Report, All India Coordinated Research Project on MULLARP (ICAR), Indian Institute of Pulses Research, Kanpur: 26.
    2. Bharathi, A., Selvaraj, K. S., Veerabadhiran, P. and Lakshmi, B. S. (2006). Crossability barriers in mungbean [Vigna radiata (L.) Wilczek) with its wild relatives. Indian J. Crop Sci. 1: 120-124.
    3. Boiling, M., Sander, D. A. and Matlock, R. S. (1961). Mungbean hybridization technique. Agron. J. 53: 54-55.
    4. Biswas, M. R. and Dana, S. (1976). Allopolyploid of Tetraploid Phaseolus species × P. calcaratus cross. Biologia Plantarum 18: 335-338.
    5. Doyle, J. J. and Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15.
    6. Dikshit, H. K., Singh, D., Singh, A., Jain, N., Kumari, J. and Sharma, T. R. (2012). Utility of adzuki bean [Vignaangularis (Willd.) Ohwi and Ohashi] simple sequence repeat (SSR) markers in genetic analysis of mungbean and related Vigna spp. Afri. J.Biotechnol. 11: 13261-13268.
    7. Gosal, S. S. and Bajaj, Y. P. S. (1983). Interspecific hybridization between Vigna mungo and Vigna radiata through embryo culture. Euphytica 32: 129-137.
    8. Nene, Y. L., Srivastava, S. K. and Naresh, J. S. (1972). Evaluation of urdbean (Phaseolus mungo L.) and mungbean (Phaseolus aureus L.) germplasms and cultivars against yellow mosaic virus.Indian J. Agric. Sci. 42: 251-254.
    9. Pal, S. S., Singh, J. J. and Singh, I. (2000). Transfer of YMV resistance in cultivar SML 32 of Vigna radiata from other related Vigna species. Pl. Disease Res. 15: 67-69.
    10. Pandiyan, M., Senthil, N., Ramamoorthi, N., Mithiah, A. R., Tomooka, N., Duncan, V. and Jayaraj, T. (2010) Interspecific hybridization of Vigna radiata x 13 wild Vigna species for developing MYMV donar. Electr. J. Plant Breed. 1: 600-610.
    11. Pandiyan, M., Senthil, N., Suresh, R., Chakravarthy, N., Packiaraj, D. and Jagdeep, S. (2012). Interspecific hybridization of Vigna radiata x Vigna trilobata. Wudpacker J. Agric. Res. 1: 233-34.
    12. Rashid, K. A., Smartt, J. and Haq, N. (1987). Hybridization in the genus Vigna. In: Proc. 2nd Int. Symp. (Ed. S. Shanmugasundram and B. T. Mclean).AVRDC.Shanhua, Taiwan. pp.205-214
    13. Satija, C. K. and Vikal, Y. (1993). Crossability and morphological studies among wild and cultivated species of Vigna. J. Cytol. Genet. 28: 173-179.
    14. Sehrawat, N., Yadav, M., Bhat, K. V., Sairam, R. K., and Jaiwal, P. K. (2016) Introgression of mungbean yellow mosaic virus resistance in Vigna mungo(L.) Hepper and purity testing of F1 hybrids using SSRs. Turkish J. Agri. and Forestry 40: 95-100.
    15. Shanmugam, A. S., Rathnaswamy, R. and Rangaswamy, S. R. (1983) Crossability studies between greengram and blackgram. Curr. Sci. 52: 1018-20.
    16. Shukla, G. P., Pandya, B. P. and Singh. D. P. (1978). Inheritance of resistance to yellow mosaic in mungbean. Indian J. Genet. 38: 358-360.
    17. Singh, D. P. (1990).Distant hybridization in genus Vigna–a review.Indian J. Genet.Plt. Breed. 50: 268-276.
    18. Sehrawat, N., Bhat, K. V., Kaga, A., Tomooka, N., Yadav, M. and Jaiwal, P. K. (2014). Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L.Wilczek). Span. J. Agric. Res. 12: 732-741.
    19. Somta, P., Kaga, A., Tomooka, N., Kashiwaba, K., Isemura, T.,Chaitieng, B., Srinives, P. and Vaughan, D. A. (2006). Development of an interspecific Vigna linkage map between Vigna umbellata (Thunb.) Ohwi & Ohashi and V. nakashimae (Ohwi) Ohwi & Ohashi and its use in analysis of bruchid resistance and comparative genomics. Pl. Breed. 125: 77-84.
    20. Subramanian, D. (1980). Interspecific hybridization in Vigna. Indian J. Genet. Plt. Breed. 40: 437-38.
    21. Tabbasam, N., Rahman, M. and Zafar, Y. (2006). DNA-based genotyping of sorghum hybrids.Pak. J.Bot. 38: 1599-1604.
    22. Tangphatsornruang, S., Somta, P., Uthaipaisanwong, P., Chanprasert, J., Sangsrakru, D., Seehalak, W., Sommanas, W. (2009). Characterization of microsatellites and gene contents from genome shotgun sequences of mungbean [Vigna radiata (L.) Wilczek] BMC Pl. Bio. 9: 137.
    23. Wu, M. F. (2001) Expression profile analysis of Hsc70 of Vigna radiata; Master’s Thesis. National TsinHua University, Hsinchu 
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