Legume Research

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Research Article

Legume Research, volume 40 issue 6 (december 2017) : 974-984

Morpho-molecular characterization of landraces/wild genotypes of Cicer for Biotic/ Abiotic stresses

Rajendra Kumar, Renu Yadav, Sangeeta Soi, Srinivasan, S.S.Yadav, Ashwani Yadav, J.P.Mishra, Neha Mittal, Neelam Yadav, Ashwani Kumar, Vaishali, Hemant Yadav, Hari D Upadhyaya
1Department of Biotechnology, S.V.P. University of Agriculture & Technology, Meerut - 250110 (Uttar Pradesh) India
Cite article:- Kumar Rajendra, Yadav Renu, Soi Sangeeta, Srinivasan, S.S.Yadav, Yadav Ashwani, J.P.Mishra, Mittal Neha, Yadav Neelam, Kumar Ashwani, Vaishali, Yadav Hemant, Upadhyaya D Hari (2017). Morpho-molecular characterization of landraces/wild genotypes of Cicer for Biotic/ Abiotic stresses. Legume Research. 40(6): 974-984. doi: 10.18805/lr.v0iOF.9100.

ABSTRACT

Chickpea is one of the most important pulse crops in the world. However, chickpea productivity is not high enough to fulfill the requirements of an ever-increasing demand. One of the major constraints in the chickpea improvement is the narrow genetic base in the cultivated chickpea (Cicer arietinum L) and its sexual incompatibility with other Cicer species. Knowledge of molecular characterization, genetic diversity, and relatedness in the germplasm is a prerequisite for varietal registration, protection and overall crop improvement. The present study reports molecular characterization of 75 genotypes using 46 STMS markers. All the STMS loci were found to be highly polymorphic. A total of 132 alleles were found with an average of 2.87 per locus. The highest numbers of alleles were observed with marker TA-21. A cluster analysis arranged these 75 genotypes in 7 clusters. The genotypes ICRISAT 3073 and Pusa 212 showed remarkable genetic similarity (0.796) and the largest genetic distance was observed between C. reticulatum wild-1 and CSG 9505 (0.547). The present analysis provides an insight into the interrelationship among the genotypes and highlights the requirement for effective supplementation of morphological data with additional molecular markers to efficiently unearth the genetic inter-relationship among the genotypes.

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