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Abiotic stresses alter expression of S-Adenosylmethionine synthetase gene, polyamines and antioxidant activity in pigeon pea (Cajanus cajan L.)

DOI: 10.18805/lr.v39i6.6640    | Article Id: LR-3697 | Page : 905-913
Citation :- Abiotic stresses alter expression of S-Adenosylmethionine synthetase gene, polyamines and antioxidant activity in pigeon pea (Cajanus cajan L.) .Legume Research.2016.(39):905-913

Nidhi Radadiya1, Vipul B. Parekh1, Bhavika Dobariya1, Lalit Mahatma2 and Mahesh K. Mahatma*1,3

maheshmahatma@gmail.com
Address :

Department of Plant Molecular Biology & Biotechnology, N.M. College of Agriculture, Navsari Agricultural University, Navsari-396 450, Gujarat, India.

Submitted Date : 2-03-2016
Accepted Date : 11-06-2016

Abstract

Expression of S-Adenosylmethionine synthetase (SAMS) gene in pigeon pea (Cajanus cajan L.) was analyzed by qRT PCR during abiotic stresses viz., drought, heavy metal (CdCl2) and cold.  Maximum expression of SAMS gene in the leaves were observed at 3 days after drought stress with 15% PEG. Conversely, its expression was not detected in leaves and roots at cadmium stress but transcripts were down regulated as compared to the control. After 6 days of stress expression of SAMS gene was increased in leaves and roots as compared to the control but it was lower than its expression at 3 days after stress. The activities of antioxidative enzymes like glutathione reductase, glutathione-s-transferase, ascorbate peroxidase and metabolite constituents like polyamines and glycine betaine were also analyzed. The activities of antioxidative enzymes and concentration of glycine betaine showed remarkable increase in response to all stresses, except ascorbate peroxidase in heavy metal stress.

Keywords

Heavy metal Pigeon pea Polyamines qRT PCR SAM synthase Water stress.

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