Characterization and identification of pigeonpea [Cajanus cajan (L.) Millsp.] genotypes based on quality of seed protein

DOI: 10.18805/LR-3696    | Article Id: LR-3696 | Page : 165-171
Citation :- Characterization and identification of pigeonpea [Cajanus cajan (L.) Millsp.] genotypes based on quality of seed protein.Legume Research.2020.(43):165-171
Ashish Kumar Pandey, Ajai Kumar Singh, Prakash Singh, Rajendra Prasad Vyas, Ravish Kumar Singh and Hemraj Bhandari prakash201288@gmail.com
Address : Department of Genetics and Plant Breeding,
Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208 002, Uttar Pradesh, India. 
Submitted Date : 2-03-2016
Accepted Date : 18-05-2019


Tris- and water-soluble seed protein profiling was used to characterize, identify and differentiate 13 genotypes of pigeonpea via SDS–PAGE based electrophoresis of seed protein. Electropherograms, RM-value and UPGMA based cluster (dendrograms) analysis was used to analyze tris- and water-soluble protein banding patterns of these genotypes. It revealed that RM-value of protein bands have differed from all the genotypes for both soluble proteins. Tris-soluble protein banding pattern exhibited 17 bands including 12-15 variate of RM-value (0.083 to 0.98) and two common bands with RM-value 0.083 and 0.78. However, water-soluble protein banding pattern revealed the presence of 16 bands including 8-12 variate of RM-value (0.16 to 0.95) with four common bands of RM-value 0.43, 048, 0.23 and 0.65, respectively. UPGMA cluster analysis was used to group the 13 genotypes into six distinct clusters. The banding pattern of tris-soluble protein was found to be more distinct than water-soluble protein. The result demonstrated that the electrophoretic profile of tris-soluble protein through SDS-PAGE was more effective than the water-soluble protein.


Pigeonpea genotype SDS–PAGE Varietal identification Tris- and water-soluble protein.


  1. Ahmad, F. and Slinkard, A.E. (1992). Genetic relationships in the genus Cicer L. as revealed by polyacrylamide gel electrophoresis of seed storage proteins. Theo. Appl. Genet. 84: 688-692.
  2. Anonymous, (2013). Production and productivity of pigeonpea, http//dacnet.nic.in, 20012-13.
  3. Asghar, R., Siddique, T. and Afza, M. (2003). Inter and intra-specific variation in SDS-PAGE electrophoregrams of total seed protein in chickpea (Cicer arietinum L.) germplasm. Pak. J. Bio. Sci. 6(24): 1991-1995.
  4. Dahiya AM, Nandwal BS (2005). Studies on genetic diversity in chickpea (Cicer arietinum L.) using total seed protein separation by SDS-PAGE. Seed Research, 33 (2): 172-178.
  5. Das, S.K. and Mukharjee, K. (1995). Comparative study on seed proteins of Ipomoea. Seed Sci Technol., 23: 501–509.
  6. Dasbak, M.A.D. and Asiegbu, J.E. (2009). Performance of pigeon pea genotypes intercropped with maize under humid tropical ultisol conditions. J. Animal and Plant Sciences, 4 (2): 329-340.
  7. DES, (2019). Ministry of Agriculture & Farmers Welfare (DAC&FW), Govt. of India; 2017-18,(https://farmer.gov.in/SucessReport2018- 19.pdf dated 20.04.2019).
  8. Gangwar, L.K. and Bajpai, G.C. (2006). Seed protein variation in interspecific hybrids of pigeon pea. Indian J. Crop Science, 1(2): 125-128.
  9. Gepts, P. (1989). Genetic diversity of seed storage proteins in plants population. Genetics, Breeding and Genetic Resources, 64–82.
  10. Ghafoor, A. and Arshad, M. (2008). Seed protein profiling of Pisum sativum L., germplasm using sodium dodecyl sulphate polyac    crylamide gel electrophoresis (SDS-PAGE) for investigation of bio-diversity. Pak. J. Bot. 40(6): 2315-2321. 
  11. Joshi, B.K., Bim, H.P., Gauchan, D., Bajracharya, J., Shrestha, P. and Upadhyay, M.P. (2009). Genetic diversity and population structure of pigeon pea. BSN E-Bulletin, 1: 1-15.
  12. Laemmli, U.K. (1970). Cleavage of structural proteins during assembly of the head bacteriophage T4. Nature, 227: 680-685. 
  13. Mandal, N. and Das, P.K. (2001). Seed protein characterization and isozyme diversity for cultivar identification in grass pea (Lathyrus sativus L.). Indian J. Genetics and Plant Breeding, 61:246-249.
  14. Mirali, N., El-Khouri, S. and Rizq, F. (2007). Genetic diversity and relationships in some Vicia species as determined by SDS-PAGE of seed proteins. Biologia Plantarum, 51 (4): 660-666.
  15. Moller, M. and Spoor, W. (1993). Discrimination and identification of Lolium species and cultivars by rapid SDS-PAGE electrophoresis of seed storage proteins. Seed Sci. Technol., 21:213–223.
  16. Murphy, R.W., Sites, W.J., Donald, G.B. and Christopher, H.H. (1996). Molecular systmatics: Proteins:Isozyme Electropheresis. Sinauer Associates, Inc., Publisher Sunderland, Massachusetts. U.S.A., 4: 51-67.
  17. Rani, C., Bishnoi, S.K., Kumar, B., Sheoran, I.S., Angrish, R. and Datta, K.S. (2007). Changes in protein profile under sodium chloride and boron toxicity stress in seedlings of two wheat cultivars. Indian J. Plant Physiology, 12(1):13-17.
  18. Salimi, S. (2013). Relationship of some soyabean genotypes based on morphological characters and biochemical markers. International J. Agronomy and Plant Production, 4(9): 2237-2243.
  19. Singh, H.P., Singh, P.V. and Sexena, R.P. (1991). Identification of chickpea cultivars using PAGE of storage seed proteins. Ann. Biol. 8: 167-175.
  20. Singh, M., Gautam, N.K., Rana, M.K., Dahiya, O.P., Dutta, M. and Bansal, K.C. (2014). Pigeonpea genetic resources and its utilization in India, current status and future prospects. J. Plant Sci. Res. 1(2): 1-8.
  21. Srivastava, D., Khan, N.A., Shamim, M., Yadav, P., Pandey, P. and Singh K.N. (2014). Assessment of the genetic diversity in bottle gourd (Lagenaria siceraria [Molina] Standl.) genotypes using SDS-Page and RAPD markers. Natl. Acad. Sci. Lett., DOI: 10.1007/s40009-013-0207-2. 
  22. Upadhyaya, H.D., Pundir, R.P.S., Gowda, C.L.L., Reddy, K.N. and Singh, S. (2005). Geographical patterns of diversity for qualitative and quantitative traits in the Pigeon pea germplasm collection. Plant Genetic Resources, 3: 331-352.
  23. Willey, R.W., Rao, M.R. and Natarajan, M. (1980). Traditional cropping systems with pigeonpea and their improvement. In Proceedings of the International Workshop on Pigeonpeas. Vol 1, 15-19 Dec. 1980, ICRISAT, Patancheru, AP, India, Pp. 11-25.

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