Legume Research

  • Chief EditorJ. S. Sandhu

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Legume Research, volume 40 issue 4 (august 2017) : 684-690

Enhancement of shelf life of liquid biofertilizer containing Rhizobium sp. infecting mungbean (Vigna radiata L.)

Anju Sehrawat*, Aarti Yadav, R.C. Anand, Kamlesh Kukreja, Sunita Suneja
1<p>Department of Microbiology,&nbsp;CCS Haryana Agricultural University, Hisar- 125 004, India.</p>
Cite article:- Sehrawat* Anju, Yadav Aarti, Anand R.C., Kukreja Kamlesh, Suneja Sunita (2017). Enhancement of shelf life of liquid biofertilizer containing Rhizobium sp. infecting mungbean (Vigna radiata L.) . Legume Research. 40(4): 684-690. doi: 10.18805/lr.v0i0.7648.

To solve the shorter shelf life, high contamination, poor quality, low field performance and processing solid carrier in carrier based bioinoculant formulation had necessitated the shifting of carrier based inoclants to liquid inoculants technology. Three different polymeric additives; polyvinyl pyrrolidone, gum arabic and glycerol were evaluated for their ability to support growth and promote survival ofliquid inoculants (Rhizobium sp. strain MB1503) during the storage. All liquid rhizobial inoculants prepared in amended media showed higher viable count in comparison to inoculants prepared in YEMB (control) at 180 d of storage. Maximum survival was observed in inoculants prepared in YEMB amended with2% PVP, 2% GA and1% GA. Mungbean (Vigna radiata L.) seeds treated with 90 d old liquid rhizobial inoculant of strain MB1503 amended with 1% PVP or 2% glycerol enhanced plant growth as compared to uninoculated control. The present investigation revealed that treatments inoculated with 90 d old inoculant + amendments (added before or after the growth) were significantly better with respect to nodule weight, shoot weight and total plant nitrogen as compared to uninoculated control/treatment.

  1. Albareda, M., Rodriguez-Navarro, D.N., Camacho, M. and Temprano, F.J. (2008). Alternatives to peat as a carrier for rhizobial inoculants: solid and liquid formulations. Soil Bio. &Biochem.40: 2717-2779.

  2. Anjum, M.S., Ahmed, Z.I. and Rauf, C.A. (2006). Effect of Rhizobium inoculation and nitrogen fertilizer on yield and yield components of mungbean. Int. J. Agric. Biol.2: 238-240. 

  3. Bhattacharjee, R. and Dey, U. (2014). Biofertilizer, a way towards organic agriculture: A review. Afr. J. Microbiol. Res.8: 2332-2342.

  4. Bhuiyan, M.A.H., Main, M.H. and Islam, M.S. (2008). Studies on the effects ofBradyrhizobium inoculation on yield and yield attributes of mungbean. Bangladesh J. Agri. Res.33: 449-457.

  5. Bremner, J.M. (1960). Determination of nitrogen in soil by Kjeldhal method. J. Agric. Sci.55: 11-13.

  6. Daniel, L.A.E., Venkateswarlu, B., Desai, S., Kumar, P.G., Ahmed, A.H.S.K., Sultana, U., Pinisetty, U. and Narasu, L.M. (2013). Effect of polymeric additives, adjuvants, surfactants on survival, stability and plant growth promoting ability of liquid bioinoculants. J. Plant Physiol. Pathol. 1: 1-5.

  7. Dayamani, K.J. and Brahmaprakash, G.P. (2014). Influence of form and concentration of the osmolytes in liquid inoculants formulations of plant growth promoting bacteria. Inter. J. Scientific Res. Publications.4: 1-6.

  8. Deaker, R., Roughley, R.J. and Kennedy, I.R. (2004). Legume seed inoculation technology- A review. Soil Biol. Biochem. 36: 1275-1288.

  9. FAO (1991). Legume inoculant production and quality control. Food and Agricultural organization of the United Nations. 145.

  10. Franca, C.R.R.S., Junior, M.A.L., Figueiredo, M.V.B. and Stiva, N.P.S.G.A. (2013). Feasibility of rhizobia conservation by liquid conditioners. Artigo Científico. 44: 661-668.

  11. Fred, E.B., Baldwin, I.L. and McCoy, E. (1932). Root Nodule Bacteria and Leguminous Plants, University of Wisconsin Press, Madison.

  12. Girisha, H.C., Brahmaprakash, G.P. and Mallesha B.C. (2006). Effect of osmoprotectant (PVP- 40) on survival of Rhizobium in different inoculants formulation and nitrogen fixation in cowpea. GEOBIOS. 33: 151-156.

  13. Islam, M.K., Islam, S.M.A., Rashid, M.H., Hossain, A.F.M.F.F. and Alom, M.M. (2006). Effect of biofertilizer and plant growth regulators on growth of summer mungbean. Int. J. Botany. 2: 36-41.

  14. Krumnow, A.A., Sorokulova, I.B., Olsen, E., Globa, L., Barbaree, J.M. and Vodyanoy, V.J. (2009). Preservation of bacteria in natural polymers. J. Microbiol. Methods.78: 189–194.

  15. Kumaresan, G. and Reetha, D. (2011). Survival of Azospirillum brasilense in liquid formulation amended with different chemical additives. J. Phyt. 3: 48-51.

  16. Mansoor, M. (2007). Evaluation of various agronomic management practices for increased productivity of mungbean (Vigna radiata L. Wilczek). Ph.D thesis. Department of Agronomy, Faculty of Agriculture Gomal University Dera Ismail Khan: 1-163. http://eprints.hec.gov.pk /2430/1/2285.htm.

  17. Ophir, T. and Gutnick, D. L. (1994). A role for exopolysaccharides in the protection of microorganisms from desiccation. Appl. Environ. Microbiol. 66: 740- 745.

  18. Prakash, O. (2010). Development and evaluation of liquid rhizobial inoculant technology for winter legumes. Ph.D. Thesis, CCSHAU, Hisar.

  19. Sehrawat, A., Suneja, S., Yadav, A. and Anand, R.C. (2015). Influence of different additives on shelf life of rhizobial inoculants for mungbean(Vigna radiata L.). Int. J. Recent Scientific Res. 6: 4338-4342.

  20. Singleton, P.W., Keyser, H.H. and Sande, E.S. (2002). Development and evaluation of liquid inoculants. In: “Inoculants and nitrogen fixation of legumes in Vietnam”. Eds. D. Herridge, ACIAR proceedings. Aust. Centre for Int. Res. 109: 52-66.

  21. Smith, R.S. (1992). Legume inoculant formulation and application. Can. J. Micrbiol. 38: 485-492.

  22. Swarnalakshmi, K., Singh, M. and Chaudhary, R.G. (2011). Comparison of adhesive agents on Rhizobium survival as well as nodulation and growth of urdbean under field conditions. J. Food Legumes 24: 332-334.

  23. Temprano, F.J., Albareda, M., Camacho, M., Daza, A., Santamaria, C. and Rodriguez-Navarro, D.N. (2002). Survival of several Rhizobium/Bradyrhizobium strains on different inoculants formulations and inoculated seeds. Int. Microbiol. 5: 81-86.

  24. Tittabutr, P., Payakapong, W., Teaumroong, N., Singleton, P.W. and Boonkerd, N. (2007). Growth, survival and field performance of bradyrhizobial liquid inoculants formulations with polymeric additives. Sci. Asia. 33: 69-77.

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