Loading...

Genetic interrelationship among cowpea varieties elucidated through morphometric, RAPD and SSR analyses

DOI: 10.18805/lr.v0iOF.3767    | Article Id: LR-3649 | Page : 409-415
Citation :- Genetic interrelationship among cowpea varieties elucidatedthrough morphometric, RAPD and SSR analyses .Legume Research.2017.(40):409-415

T. Pradeepkumar*, Deepu Mathew, C. Varun Roch, K. Veni  and K.R. Midhila

pradeepkumar.t@kau.in
Address :

Hi-Tech seed Testing Laboratory, Department of Olericulture, Kerala Agricultural University, KAU Post, Thrissur – 680 656, India.

Submitted Date : 6-11-2015
Accepted Date : 19-03-2016

Abstract

The genetic interrelationship among all the popular vegetable cowpea varieties cultivated in Kerala was investigated through morphometric, RAPD and SSR analyses. High polymorphism was observed in RAPD (88.0 per cent of bands were polymorphic) and SSR (80.0 per cent of bands were polymorphic) analyses. Dendrogram based on morphological parameters has divided the genotypes into two main clusters which truncated at 68.0 per cent similarity, with purple and green poded varieties segregating into separate clusters. Morphometric analysis was successful to group the varieties with determinate and indeterminate growth habits whereas, RAPD and SSR markers could not make a clear division. However, distinction was apparent between purple poded and green poded types and purple poded varieties viz Vyjayanthi and Varun exhibited high similarity with each other in SSR analysis (80.0 per cent) when compared to RAPD (77.0 per cent). Dendrogram of pooled data of SSR and RAPD analyses has revealed maximum similarity between Vyjayanthi and Varun (77.0 per cent).
 

Keywords

Genetic diversity Molecular marker RAPD SSR Vigna.

References

  1. Backiyarani, S., Nadarajan, N., Rajendran, C. and Shanthi, S. (2000). Genetic divergence for physiological traits in cowpea [Vigna unguiculata. (L.) Walp.]. Legume Res., 23: 114-117.
  2. Bered, F., Terra, T.F., Spellmeier, M. and Neto, J.F.B. (2005). Genetic variation among and within sweet corn populations detected by RAPD and SSR markers. Crop Breed. Appl. Biotechnol., 5: 418-425.
  3. Demeke, T., Lynch, D.R., Kawchuck, L.M., Kozub, G.C. and Armstrong, J.D. (1996). Genetic diversity of potato determined by random amplified polymorphic DNA analysis. Plant Cell Rep., 15: 662-667.
  4. Diouf, D. and Hilu, K.W. (2005). Microsatellites and RAPD markers to study genetic relationship among cowpea breeding lines and local varieties in Senegal. Genet. Resour. Crop Evol., 52: 1057-1067.
  5. Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus., 12 : 13-15. 
  6. Fatokun, C.A., Danesh, D. and Young, N.D. (1993). Molecular taxonomic relationships in the genus Vigna based on RFLP analysis. Theor. Appl. Genet., 86: 97–104.
  7. Gopalakrishnan, T.R. and Devadas, V.S. (2014). Crop Varieties from Kerala Agricultural University, Directorate of Research, Kerala Agricultural University, Thrissur, India., pp. 146.
  8. IBPGR. (1983). International Board for Plant Genetic Resources., Descriptors for Cowpea. Rome.
  9. Khan, M.T., Reza, M.O.H., Kha, M.A., Haqu, M.S., Isla, M.S. and Kha, M.B. (2015). Genetic diversity analysis of cowpea by RAPD markers. Int. J. Innov. Appl. Stud, 10: 459-465. 
  10. Ndoye, A.L., Sadio, O. and Diou, D. (2011). Genetic variation of Casuarina equisetifolia subsp equisetifolia and C. equisetifolia subsp incana populations on the northern coast of Senegal. Genet. Mol. Res., 36-46.
  11. Ogunkanmi, L.A., Ogundipe, O.J. and Fatokun, C.A. (2014). Molecular characterization of cultivated cowpea (Vigna unguiculata (L.) Walp.]. using simple sequence repeats markers. African J. Biotechnol., 13: 3364-3472.
  12. Pandey, R.N., Dhanasekar, P. and Souframanien, J. (2004). RAPD-based DNA fingerprinting and analysis of genetic diversity in radiation-induced mutants of cowpea [Vigna unguiculata (L.) Walp.]. Indian J. Genet. Plant Breed., 64: 97- 101.
  13. Panella, L. and Gepts, P. (1992). Genetic relationships within Vigna unguiculata (L.) Walp. based on isonzyme analyses. Genet. Resour. Crop. Evol., 39: 71–88.
  14. Pasquet, R.S. (2000). Allozyme diversity of cultivated cowpea [Vigna unguiculata (L.) Walp]. Theor. Appl. Genet., 101: 211–219.
  15. Rohlf, F.J. (2000). NTSYS-pc. Numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Publ., Setauket, NY.
  16. Senior, M.L., Murphy, J.P., Goodman, M.M. and Stuber, C.W. (1998). Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system. Crop Sci., 38: 1088-1098.
  17. Sharawy, W.M. and Fiky, Z.A. (2003). Characterization of cowpea (Vigna unguiculata L.) genotypes based on yield traits and RAPD–PCR analysis. Arab J. Biotech., 6: 67-78.
  18. Vaillancourt, R.F., Weeden, N.F. and Barnard, J. (1993). Isoenzyme diversity in the cowpea species complex. Crop Sci., 33: 606–613.
  19. Webster, M.T., Smith, N.G.C. and Ellegren, H. (2002). Microsatellite evolution inferred from human-chimpanzee genomic sequence alignments. Proc. Natl. Acad. Sci. USA., 99: 8748–8753.
  20. Xu, P., Wu, X., Wang, B., Liu, Y., Qin, D., Ehlers, J.D., Close, T.J., Hu, T., Lu, Z. and Li, G. (2010). Development and polymorphism of Vigna unguiculata ssp. unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp. sesquipedialis (L.) Verdc.). Mol. Breed., 25: 675-684.
  21. Zannou, A., Kossou, D.K., Ahanchede, A., Zoundjihekpon, J., Agbicodo, E., Struik, P.C. and Sanni, A. (2008). Genetic variability of cultivated cowpea in Benin assessed by random amplified polymorphic DNA. African J. Biotechnol., 7: 4407- 4414.
     

Global Footprints