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Amelioration of salt stress in chickpea (Cicer arietinum L.) by coinculation of ACC deaminase-containing rhizospheric bacteria with Mesorhizobium strains

DOI: 10.18805/lr.v0iOF.9382    | Article Id: LR-3558 | Page : 80-86
Citation :- Amelioration of salt stress in chickpea (Cicer arietinum L.) by coinculation of ACC deaminase-containing rhizospheric bacteria with Mesorhizobium strains .Legume Research.2017.(40):80-86

Deepika Chaudhary and Satyavir Singh Sindhu*

sindhuss@hau.ernet.in
Address :

Department of Microbiology, CCS Haryana Agricultural University, Hisar- 125 004, India.

Submitted Date : 9-07-2015
Accepted Date : 8-10-2015

Abstract

Chickpea is a major legume crop grown in the semi-arid tropics and its yields are adversely affected by salinity. In this study, 55 rhizobacterial isolates obtained from the chickpea rhizosphere soil were screened for their salt tolerance. At 3% NaCl concentration, 41.8% rhizobacterial isolates formed colonies varying from 0.5-10 mm size and only 10.9 per cent isolates showed growth at 4% NaCl concentration. Significant growth on ACC supplemented medium plates was observed in 32.7% rhizobacterial isolates. Coinoculation studies with ACC+ as well as ACC- Mesorhizobium and rhizobacterial isolates were made on chickpea under chillum jar conditions containing sloger’s broth with salt (EC, 4dS/m) and without salt. Coinoculation of Mesorhizobium isolate MBD26 (ACC+) and rhizobacterial isolate RHD18 (ACC+) formed 59 nodules/plant and caused 112.9% increase in plant dry weight as compared to uninoculated control plants at 50 days of plant growth, whereas in the presence of salt, only 31.2% increase in plant dry weight was observed in comparison to uninoculated plants. At 80 days of plant growth, coinoculation of Mesorhizobium isolate MBD26 (ACC+) with rhizobacterial isolate RHD18 (ACC+) further increased the nodule number (78 nodules/plant) and 141.9% increase in shoot dry weight was observed as compared to uninoculated plants. Thus, it was concluded that coinoculation of ACC+ Mesorhizobium and rhizobacterial isolates showed more stimulatory effect on nodulation and plant biomass under normal and salt amended treatments. 

Keywords

Chickpea Mesorhizobium Nodulation Rhizobacterial isolates Salinity Shoot dry weight.

References

  1. Aamir, M., Aslam, A., Khan, M.Y., Jamshaid, M.U., Ahmad, M., Asghar, H.N. and Zahir, A.Z. (2013). Coinoculation with Rhizobium and plant growth promoting rhizobacteria (PGPR) for inducing salinity tolerance in mung bean under field condition of semi-arid climate. Asian J. Agri. Biol., 1: 17–22. 
  2. Ahmad, M., Zahir, Z.A. and Asghar, H.N. (2011). Inducing salinity tolerance in mung bean through rhizobia and plant growth promoting rhizobacteria containing 1-aminocyclopropane 1- carboxylate deaminase. Can. J. Microbiol., 57: 578–589.
  3. Arshad, M. and Frankenberger, Jr. W.T. (2002). Ethylene: agricultural sources and applications. Kluwer Academic Publishers, New York, USA. pp. 415–438.
  4. Arshad, M., Shaharoona, B. and Mahmood, T. (2008). Inoculation with Pseudomonas spp. containing ACC deaminase partially eliminates the effects of drought stress on growth, yield and ripening of pea (Pisum sativum L.). Pedosphere, 18: 611–620.
  5. Ashraf, M., Athar, H. R., Harris, P.J.C. and Kwon, T. R. (2008). Some prospective strategies for improving salt tolerance. Adv. Agron., 97: 232–240.
  6. Bekki, A., Trinchant, J.C. and Rigaud, J. (1987). Nitrogen fixation (C2H4 reduction) by Medicago nodules and bacteroids under sodium chloride stress. Physiol. Plant., 71: 61–67.
  7. Dahiya, J.S. and Khurana, A.L. (1981). Chillum jar, a better technique for screening of rhizobia under summer conditions. Plant Soil, 83: 299–302.
  8. Delgado, M.J., Ligero, F. and Lluch, C. (1994). Effect of salt stress on growth and nitrogen fixation by pea, faba-bean, common bean and soybean plants. Soil Biol. Biochem., 26: 371–376.
  9. Duan, J., Müller, K.M., Charles, T.C., Vesely, S. and Glick, B.R. (2009). 1-Aminocyclopropane-1-carboxylate (ACC) deaminase genes in rhizobia from southern Saskatchewan. Microbiol. Ecol., 57: 423–436.
  10. Egamberdieva, D. and Kucharova, Z. (2009). Selection for root colonising bacteria stimulating wheat growth in saline soils. Biol. Fertil. Soils, 45: 563–571.
  11. Gauri., Singh, A.K. and Bamania, M. (2012). Characterization of Mesorhizobium sp. isolated from root nodules of Cicer arietinum. Intern. J. Agril. Sci. Res., 2: 142–154.
  12. Glick, B.R. (2014). Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol. Res., 169: 30–39.
  13. Glick, B.R., Cheng, Z., Czarny, J. and Duan, J. (2007). Promotion of plant growth by ACC deaminase-containing soil bacteria. Eur. J. Plant Pathol., 119: 329–339.
  14. Jalill, F., Khavazi, K., Pazira, E., Nejati, A., Tahmani, H. A., Sadaghiani, H. R. and Miransari, M. (2009). Isolation and characterization of ACC deaminase producing fluorescent pseudomonads, to alleviate salinity stress on canola (Brassica napus L.) growth. J. Plant Physiol., 166: 667–674.
  15. Khandelwal, A. and Sindhu, S.S. (2012). Expression of 1-aminocyclopropane-1-carboxylate deaminase in rhizobia promotes nodulation and plant growth of clusterbean (Cyamopsis tetragonoloba L.). Res. J. Microbiol., 7: 158–170.
  16. Khandelwal, A. and Sindhu, S.S. (2013). ACC deaminase containing rhizobacteria enhance nodulation and plant growth in clusterbean (Cyamopsis tetragonoloba L.). J. Microbiol. Res., 3: 117–123.
  17. Lindner, R.C. (1944). Rapid analytical method for some of the more common inorganic constituents of plant tissues. Plant Physiol., 19: 76–79.
  18. Marsudi, N.D.S., Glenn, A.R. and Dilworth, M.J. (1999). Identification and characterization of fast and slow growing root nodule bacteria from south western Australian soils able to nodulate Acacia saligna. Soil Biol. Biochem., 31: 1229–1238. 
  19. Mayak, S., Tirosh, T. and Glick, B.R. (2004). Plant growth-promoting bacteria that confer resistance in tomato plants to salt stress. Plant Physiol. Biochem., 42: 565–572.
  20. Munns, R. and Tester, M. (2008). Mechanisms of salinity tolerance. Annu. Rev. Plant Biol., 59: 651–681.
  21. Nukui, N., Ezura, H., Yuhashi, K.I., Yasuta, T. and Minamisawa, K. (2000). Effects of ethylene precursor and inhibitors for ethylene biosynthesis and perception on nodulation in Lotus japonicus and Macroptillium atropurpureum. Plant Cell Physiol., 41: 893–897. 
  22. Omar, M.N.A., Osman, M.E.H., Kasim, W.A. and El-Daim, I.A. Abd (2009). Improvement of salt tolerance mechanisms of barley cultivated under salt stress using Azospirillum brasilense. Tasks Vegetation Sci., 44: 133–147. 
  23. Penrose, D.M. and Glick, B.R. (2003). Methods for isolating and characterizing ACC deaminase -containing plant growth-    promoting rhizobacteria. Physiol. Plant., 118: 10–15. 
  24. Pii, Y., Mimmo, T., Tomasi, N., Terzano, R., Cesco, S. and Crecchio, C. (2015). Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. A review. Biol. Fertil. Soils, 51: 403–415.
  25. Plaut, Z., Edelstein, M. and Ben-Hur, M. (2013). Overcoming salinity barriers to crop production using traditional methods. Crit. Rev. Plant Sci., 32: 250–291. 
  26. Roy, B., Noren, S.K., Mandal, A.B. and Basu, A.K. (2011). Genetic engineering for abiotic stress tolerance in agricultural crops. Biotechnology, 10: 1–22.
  27. Serraj, R., Roy, G. and Drevon, J. (1994). Salt stress induces a decrease in the oxygen uptake of soybean nodules and their permeability to oxygen diffusion. Physiol. Plant., 91: 61–68.
  28. Shaharoona, B., Arshad, M. and Zahir, Z.A. (2006). Effect of plant growth promoting rhizobacteria containing ACC-    deaminase on maize (Zea mays L.) growth under axenic conditions and on nodulation in mung bean (Vigna radiata L.). Lett. Appl. Microbiol., 42: 155–159. 
  29. Sindhu, S.S., Dua, S. Verma, M.K. and Khandelwal, A. (2010). Growth promotion of legumes by inoculation of rhizosphere bacteria. In: Microbes for Legume Improvement. Khan, M.S., Zaidi, A. and Musarrat, J. eds., Springer-Wien/    NewYork, Germany. pp. 195–235.
  30. Sindhu, S.S., Gupta, S.K. and Dadarwal, K.R. (1999). Antagonistic effect of Pseudomonas spp. on pathogenic fungi and enhancement of plant growth in green gram (Vigna radiata). Biol. Fertil. Soils, 29: 62–68. 
  31. Sloger, C. (1969). Symbiotic effectiveness and nitrogen fixation in nodulated soybean. Plant Physiol., 44: 1666–1668.
  32. Timmusk, S., Paalme, V.T., Bergquist, J., Vangala, A., Danilas, T. and Nevo, E. (2011). Bacterial distribution in the rhizosphere of wild barley under contrasting microclimates. PLoS ONE 23: 6(3):e17968. doi: 10.1371/journal.pone.0017968
  33. Upadhyay, S., Sanjeev, K. and Devendra, P. (2012). Salinity tolerance in free living plant growth promoting rhizobacterial. Indian J. Sci. Res., 3: 73–78.
  34. Wang, Y.R., Kang, S.Z., Li, F.S., Zhang, L. and Zhanf, J.H. (2007). Saline water irrigation through a crop-water-salinity production function and a soilwater-salinity dynamic model. Pedosphere, 17: 303–317. 
  35. Wu, C.H., Bernard, S.M., Anderson, G.L. and Chen, W. (2009). Developing microbe-plant interactions for application in plant growth promotion and disease control, production of useful compounds, remediation and carbon sequestration. Microbiol. Biotechnol., 2: 428–440.
  36. Yang, J., Kloepper, J.W. and Ryu, C.M. (2008). Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci., 14: 1–4.

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