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Regeneration of plantlets from immature explants culture in [Glycine max (L.) Merrill]

DOI: 10.18805/lr.v0i0.7020    | Article Id: LR-3548 | Page : 69-73
Citation :- Regeneration of plantlets from immature explantsculture in [Glycine max (L.) Merrill] .Legume Research.2017.(40):69-73

Nidhi Pathak*1, Sharad Tiwari2 and Manish Kumar Mishra1 

nidhijnkvv@gmail.com
Address :

Department of Plant Breeding and Genetics, Jawahar Lal Nehru Krishi Vishwa Vidhyalaya, Jabalpur-482 004, M.P., India.

Submitted Date : 19-06-2015
Accepted Date : 25-06-2016

Abstract

For plant regeneration through somatic embryogenesis, immature cotyledonary nodes and embryonic axes explants of soybean cultivars JS 335 were cultured on six different medium fortified with MS salts, B5 vitamins and combinations of growth regulators. Between explants, immature embryonic axes demonstrated better regeneration potential as compared to cotyledonary node. For immature embryonic axes culture, better morphogenic callus induction, embryogenesis, organogenesis and plantlet regeneration was observed on MS with 4 B1.86N, MS 10N, MS .1B1N and MS.2B1N as compared to other media formulations. Cotyledonary nodes exhibited higher callus induction, embryogenesis, organogenesis on media with MS4 B1.86N, MS .1B1N, MS 3B6N and higher plantlet regeneration on media with MS.5B1N. 

Keywords

Immature embryonic axes Immature cotyledonary nodes Regeneration Soybean Somatic embryogenesis.

References

  1. Ahloowalia B.S. (1991). Somatic embryos in monocots. Their genesis and genetic stability. Rev. Cytol. Biol. Veget. Bot. 14: 223-225.
  2. Amberger L.A., Palmer, R.G. and Shoemaker, R.C. (1992). Analysis of culture-induced variation in soybean. Crop Sci. 32: 1103-1108.
  3. Anonymous, (2014). The Soybean Processors Association of India.
  4. Bailey M.A., Boerma, H.R. and Parrott, W.A. (1993). Genotype-specific optimization of plant regeneration from somatic embryos of soybean. Plant Sci. 93: 117-120.
  5. Beversdorf W.D. and Bingham, E.T. (1977). Degrees of differentiation obtained in tissue cultures of Glycine species. Crop Sci. 17: 307-311.
  6. Cheng T.Y., Saka, H. and Voqui-Dinh, T.H. (1980). Plant regeneration from soybean cotyledonary node segment in culture. Plant Sci. Lett. 19: 91-99.
  7. Christianson M.L., Warnick, D.A. and Carlson, P.S. (1983). A morphogenetically competent soybean suspension culture. Science 222: 632-634.
  8. Gaj M.D., (2001). Direct somatic embryogenesis as a rapid and efficient system for in vitro regeneration of Arabidopsis thaliana. Plant Cell Tiss. Organ Cult. 64: 39-46.
  9. Henry R.J., Nato, A. and De Buyser, J. (1998). Genetic fidelity of plants regenerated from somatic embryos of cereals. In: Jain SM, Brar DS, Ahloowalia BS (eds) Somaclonal variation and induced mutations in crop improvement. Kluwer Academic Publishers, Dordrecht, Netherlands, pp 65-80
  10. Lazzeri P.A., Hilderbr, D.F. and Collins, G.B. (1985). Soybean somatic embryogenesis: Effects of hormones and culture manipulations Plant cell Tissue organ cult. 10:197-200.
  11. Lazzeri P.A., Hildebrand, D.F., Sunega, J., Williams, E.G. and Collins, G.B. (1988). Soybean somatic embryogenesis: Interactions between sucrose and auxin. Plant Cell Rep. 7: 517-520
  12. Lippmann B. and Lippmann, G. (1984). Induction of somatic embryos in cotyledons tissue of soybean Glycine max (L.) Merrill. Plant Cell Rep. 3: 215-218.
  13. Liu W., More, P.J. and Collins, G.B. (1992). Somatic embryogenesis in soybean via somatic embryo cycling. In vitro Cell Dev. Biol. 28: 153-160
  14. Mc Kently A.H., (1991). Direct somatic embryogenesis from axes mature preenut embryos. In vitro. cell Dev. Biol., 27: 197-200. 
  15. Murashige T. and Skoog, F. (1962). A revised medium for rapid growth and biassays with tobacco tissue cultures. Plant Physiol. 15: 477-497. 
  16. Parrot W.A., Dryden, G., Vogt, S., Hildebr, D.F., Collins, G.B. and Williams, E.G. (1988). Optimization of somatic embryogenesis and embryo germination in soybean. In vitro Cell Dev. Biol. 4: 817-820.
  17. Pathak N., (2009). Genetic transformation studies in Soybean (Glycine max L.) for yellow mosaic virus resistance. Msc. Thesis, Jawahar Lal Nehru Krishi Vishwa Vidhyalaya, JBP, MP, India.
  18. Pathak N., Tiwari, S., Mishra, M. K. and Gautam, S. S. (2014). Somatic embryogenesis studies in soybean [Glycine max (L.) Merr.] In: Proceedings of National conference on biotechnology 8,9 september 2014 Jabalpur, MP. Pp 41.
  19. Ranch J.P., Ogelshy, L. and Zielinski, A.C. (1986). Plant regeneration of somatic embryos from all suspension cultures of soybean In vitro cell Dev Biol. 21: 653-658. 
  20. Reddy R.I. and Reddy, G.M. (1993). Factors affecting direct somatic embryo genesis and plant regeneration in groundnut (Arachis hypogea L.). India J. Exp Biol, 33: 57- 60.
  21. Sato S., Newell, C., Kolacz, K., Tredo, L., Finer, J., Hinchee, M. (1993). Stable transformation via particle bombardment in two different soybean regeneration system. Plant Cell Rep. 12: 408-413.
  22. Snedecor G.W. and Cochran, W.G. (1967). Statistical Methods VI Ed. Oxford IBH Pub. Co. Delhi.
  23. Ugandhar T., Venkateshwarlu, M.,. Parvathi, D., Shekar, G. P. V., Srilatha, T. and Reddy, J. K. (2011). High frequency somatic embryogenesis and plantlet regeneration from shoot tip explants of Soybean. Science Research Rep. 1: 146-150.
  24. Tian L.N.M., Brown, P.L., Voldeng, H. and Webb, J. (1994). In vitro response and pedigree analysis for somatic embryo genesis of long day photoperiod ‘adopted soybean. Plant cell Tissue Organ cult., 36: 269-273.
  25. Tripathi M.K. and Tiwari, S. (2003). Epigenesis and High Frequency Plant Regeneration from Soybean (Glycine max (L.) Merr.) Hypocotyls. Plant Tissue Cult. 13 : 61-73.
  26. Walker D.R. and Parrott, W.A. (2001). Effect of polyethylene glycol and sugar alcohols on soybean somatic embryo germination and conversion. Plant Cell Tissue Organ Cult. 64: 55-62
  27. Wright M.S., Koehler, S.M., Hinchee, M.A. and Carnes M.G. (1986). Plant regeneration by organogenesis in Glycine max. Plant Cell Rep 5: 150-154.
  28. Wu C., Chiera, J.M., Ling, P.P. and Finer, J.J. (2008). Isoxaflutole treatment leads to reversible tissue bleaching and allows for more effective detection of GFP in transgenic soybean tissues. In vitro Cellular and Developmental Biology–    Plant, 44: 540–547.
     

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